CA2448534A1 - Analyse peptidique au moyen d'un support solide - Google Patents

Analyse peptidique au moyen d'un support solide Download PDF

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Publication number
CA2448534A1
CA2448534A1 CA002448534A CA2448534A CA2448534A1 CA 2448534 A1 CA2448534 A1 CA 2448534A1 CA 002448534 A CA002448534 A CA 002448534A CA 2448534 A CA2448534 A CA 2448534A CA 2448534 A1 CA2448534 A1 CA 2448534A1
Authority
CA
Canada
Prior art keywords
polypeptide
ester
reagent
peptide
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002448534A
Other languages
English (en)
Inventor
Harold L. Vaughn
Robert S. Youngquist
Maria Liminga
Thomas W. Keough
Ronnie Palmgren
Ramagauri Bhikhabhai
Jean-Luc Maloisel
Ken E. Yelm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Amersham Bioscience AB
Procter and Gamble Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/863,786 external-priority patent/US7074570B2/en
Application filed by Amersham Bioscience AB, Procter and Gamble Co filed Critical Amersham Bioscience AB
Publication of CA2448534A1 publication Critical patent/CA2448534A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne une méthode d'identification d'un polypeptide. Cette méthode consiste (a) à effectuer la dérivatisation de la terminaison N du polypeptide, ou des terminaisons N d'un ou de plusieurs peptides de ce polypeptide, avec au moins un réactif acide comprenant une fraction sulfonyle couplée à une fraction acide activée en vue d'obtenir un ou plusieurs dérivés peptidiques, (b) à analyser l'un au moins de ces dérivés au moyen d'une technique spectrométrique de masse en vue de produire un modèle de fragmentation, et (c) à interpréter le modèle de fragmentation ainsi obtenu, le peptide ou le polypeptide étant immobilisé sur un support solide au moins pendant l'étape (a). Par ailleurs, la présente invention concerne une trousse destinée à identifier un polypeptide au moyen d'une technique spectrométrique de masse.
CA002448534A 2001-05-23 2002-05-20 Analyse peptidique au moyen d'un support solide Abandoned CA2448534A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/863,786 2001-05-23
US09/863,786 US7074570B2 (en) 2001-05-23 2001-05-23 Peptide fragmentation
US34301901P 2001-12-20 2001-12-20
US60/343,019 2001-12-20
PCT/US2002/016247 WO2002095419A2 (fr) 2001-05-23 2002-05-20 Analyse peptidique au moyen d'un support solide

Publications (1)

Publication Number Publication Date
CA2448534A1 true CA2448534A1 (fr) 2002-11-28

Family

ID=26993298

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002448534A Abandoned CA2448534A1 (fr) 2001-05-23 2002-05-20 Analyse peptidique au moyen d'un support solide

Country Status (5)

Country Link
EP (1) EP1434999A2 (fr)
JP (1) JP2005518521A (fr)
AU (1) AU2002311995A1 (fr)
CA (1) CA2448534A1 (fr)
WO (1) WO2002095419A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR116100A0 (en) 2000-11-01 2000-11-23 University Of Sydney, The P450 gene regulation
GB0320415D0 (en) * 2003-08-30 2003-10-01 Shimadzu Res Lab Europe Ltd Analysis of macromolecules
US7371514B2 (en) 2004-07-16 2008-05-13 Agilent Technologies, Inc. Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry
WO2008074067A1 (fr) * 2006-12-18 2008-06-26 Macquarie University Détection et quantification de polypeptides par spectrométrie de masse
HRP20100044B1 (hr) 2010-01-25 2016-11-18 Institut Ruđer Bošković Metoda identifikacije proteina spektrometrijom masa
WO2019202872A1 (fr) * 2018-04-16 2019-10-24 株式会社島津製作所 Kit de spectrométrie de masse, kit d'identification de micro-organisme, procédé de préparation d'échantillon, procédé d'analyse et procédé d'identification de micro-organisme

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3478013A (en) * 1966-08-22 1969-11-11 Abbott Lab Carbohydrate derivatives of erythromycin
SU818489A3 (ru) * 1978-10-04 1981-03-30 Шеринг Аг (Фирма) Способ получени кортикоид -21- СульфОпРОпиОНАТОВ или иХ СОлЕй
WO2000043792A2 (fr) * 1999-01-20 2000-07-27 The Procter & Gamble Company Procedes permettant de sequencer des polypeptides et kits a cet effet

Also Published As

Publication number Publication date
JP2005518521A (ja) 2005-06-23
AU2002311995A1 (en) 2002-12-03
WO2002095419A2 (fr) 2002-11-28
EP1434999A2 (fr) 2004-07-07
WO2002095419A3 (fr) 2003-04-24

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Legal Events

Date Code Title Description
FZDE Discontinued