CA2443978A1 - Electrophoretic separation system - Google Patents
Electrophoretic separation system Download PDFInfo
- Publication number
- CA2443978A1 CA2443978A1 CA002443978A CA2443978A CA2443978A1 CA 2443978 A1 CA2443978 A1 CA 2443978A1 CA 002443978 A CA002443978 A CA 002443978A CA 2443978 A CA2443978 A CA 2443978A CA 2443978 A1 CA2443978 A1 CA 2443978A1
- Authority
- CA
- Canada
- Prior art keywords
- separation
- fluid
- separation medium
- analytes
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 248
- 239000012530 fluid Substances 0.000 claims abstract description 175
- 230000004888 barrier function Effects 0.000 claims abstract description 65
- 238000007789 sealing Methods 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims abstract description 41
- 238000004891 communication Methods 0.000 claims abstract description 19
- 230000005684 electric field Effects 0.000 claims description 46
- 239000012491 analyte Substances 0.000 claims description 41
- 238000001962 electrophoresis Methods 0.000 claims description 38
- 239000000203 mixture Substances 0.000 claims description 23
- 238000013508 migration Methods 0.000 claims description 9
- 230000005012 migration Effects 0.000 claims description 9
- 238000011144 upstream manufacturing Methods 0.000 claims description 8
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 238000003860 storage Methods 0.000 claims description 5
- 239000000499 gel Substances 0.000 description 88
- 239000000523 sample Substances 0.000 description 66
- 239000007788 liquid Substances 0.000 description 30
- 239000000872 buffer Substances 0.000 description 20
- 239000000463 material Substances 0.000 description 13
- 239000007853 buffer solution Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 229920000936 Agarose Polymers 0.000 description 9
- 238000001816 cooling Methods 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 238000009826 distribution Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 5
- 239000012809 cooling fluid Substances 0.000 description 5
- 238000001502 gel electrophoresis Methods 0.000 description 5
- 229920002401 polyacrylamide Polymers 0.000 description 5
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 239000000110 cooling liquid Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 239000004411 aluminium Substances 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
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- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000012146 running buffer Substances 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 229920005439 Perspex® Polymers 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000003999 initiator Substances 0.000 description 2
- 238000001155 isoelectric focusing Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 229920003051 synthetic elastomer Polymers 0.000 description 2
- 239000005061 synthetic rubber Substances 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- 101100443249 Caenorhabditis elegans dig-1 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002943 EPDM rubber Polymers 0.000 description 1
- 241001547070 Eriodes Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- -1 for instance Substances 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 239000012216 imaging agent Substances 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229920003052 natural elastomer Polymers 0.000 description 1
- 229920001194 natural rubber Polymers 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 239000003566 sealing material Substances 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0109351.7A GB0109351D0 (en) | 2001-04-17 | 2001-04-17 | Analyte separation system |
| GB0109351.7 | 2001-04-17 | ||
| GB0203853.7 | 2002-02-19 | ||
| GBGB0203853.7A GB0203853D0 (en) | 2001-04-17 | 2002-02-19 | Analyte separation system |
| PCT/GB2002/001749 WO2002084273A1 (en) | 2001-04-17 | 2002-04-16 | Electrophoretic separation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2443978A1 true CA2443978A1 (en) | 2002-10-24 |
Family
ID=26245980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002443978A Abandoned CA2443978A1 (en) | 2001-04-17 | 2002-04-16 | Electrophoretic separation system |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20040129567A1 (enExample) |
| EP (1) | EP1379864A1 (enExample) |
| JP (1) | JP2004524540A (enExample) |
| CA (1) | CA2443978A1 (enExample) |
| WO (1) | WO2002084273A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5079509B2 (ja) * | 2004-09-02 | 2012-11-21 | ライフインド・イーエイチエフ | 核酸フラグメントの二次元鎖数依存及び長さ依存分離 |
| ITPD20040301A1 (it) * | 2004-11-26 | 2005-02-26 | Dimensional Srl P | Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale |
| EP1712903B1 (en) | 2005-04-11 | 2015-07-15 | Roche Diagnostics GmbH | Method for Integrated 2D gel electrophoresis |
| US20070017808A1 (en) * | 2005-05-27 | 2007-01-25 | Intel Corporation | Linear valve-coupled two-dimensional separation device and separation matrix and method |
| EP1801573A1 (en) | 2005-12-21 | 2007-06-27 | Boehringer Mannheim Gmbh | Method and apparatus for parallel two-dimensional electrophoresis |
| EP1804058A1 (en) * | 2005-12-28 | 2007-07-04 | Roche Diagnostics GmbH | Integrated two-dimensional gel electrophoresis |
| GB2440749B (en) * | 2006-05-26 | 2011-04-06 | Marc Baumann | Multi-dimensional analysis |
| EP2159573A1 (en) | 2008-08-28 | 2010-03-03 | Koninklijke Philips Electronics N.V. | 2D electrophoresis device and method of manufacturing |
| US9005417B1 (en) * | 2008-10-01 | 2015-04-14 | Sandia Corporation | Devices, systems, and methods for microscale isoelectric fractionation |
| US20100126863A1 (en) * | 2008-11-21 | 2010-05-27 | Stewart Jr Eugene W | System and method for fully automated two dimensional gel electrophoresis |
| GB201102385D0 (en) | 2011-02-10 | 2011-03-30 | Biocule Scotland Ltd | Two-dimensional gel electrophoresis apparatus and method |
| KR101246216B1 (ko) | 2011-05-18 | 2013-03-21 | 단국대학교 산학협력단 | 원전 냉각수 분석 장치 및 원전 냉각수 분석 방법 |
| US12479884B2 (en) | 2019-08-12 | 2025-11-25 | Yale University | Intragel well sample loading system |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2365284C2 (de) * | 1973-12-31 | 1975-10-23 | Medac Gesellschaft Fuer Klinische Spezialpraeparate Mbh, 2000 Hamburg | Kammer und Verfahren für die Durchführung des zweiten Kreuz-Elektrophoreseschrittes |
| US4483885A (en) * | 1982-09-30 | 1984-11-20 | E. I. Du Pont De Nemours & Company | Method and device for electrophoresis |
| JPS62168044A (ja) * | 1986-01-20 | 1987-07-24 | Shimadzu Corp | 二次元電気泳動装置 |
| US4874490A (en) * | 1988-11-04 | 1989-10-17 | Bio-Rad Laboratories, Inc. | Pre-cast gel systems for two-dimensional electrophoresis |
| US4954236A (en) * | 1989-06-28 | 1990-09-04 | Bio-Rad Laboratories, Inc. | Apparatus and method for gel casting and electrophoresis in a single enclosure |
| DE4244082C2 (de) * | 1992-12-24 | 1994-11-03 | Etc Elektrophorese Technik Wes | Verfahren zur hochauflösenden Zweidimensional-Elektrophorese und Vorrichtung zur Durchführung des Verfahrens |
| US5534121A (en) * | 1994-05-16 | 1996-07-09 | The United States Of America As Represented By The Department Of Health And Human Services | Preparative two dimensional gel electrophoresis system |
| US5525202A (en) * | 1994-09-20 | 1996-06-11 | E-C Apparatus Corporation | Assembly method of and glass plate cassette for electrophoresis |
| US5837116A (en) * | 1996-07-12 | 1998-11-17 | California Institute Of Technology | Two dimensional electrophoresis apparatus |
| US5773645A (en) * | 1997-05-05 | 1998-06-30 | Bio-Rad Laboratories, Inc. | Two-dimensional electrophoresis device |
| US5993627A (en) * | 1997-06-24 | 1999-11-30 | Large Scale Biology Corporation | Automated system for two-dimensional electrophoresis |
| US6013165A (en) * | 1998-05-22 | 2000-01-11 | Lynx Therapeutics, Inc. | Electrophoresis apparatus and method |
| DE19831210A1 (de) * | 1998-07-03 | 2000-01-05 | Wita Gmbh Wittmann Inst Of Tec | Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen |
| EP1117992A1 (en) * | 1998-08-31 | 2001-07-25 | C.B.S. Scientific Co., Inc. | Two dimensional gel electrophoresis system |
| DE19930253A1 (de) * | 1999-06-25 | 2000-12-28 | Wita Gmbh Wittmann Inst Of Tec | Kombinationskammer und Verfahren zur Trennung von Biomolekülen |
-
2002
- 2002-04-16 WO PCT/GB2002/001749 patent/WO2002084273A1/en not_active Ceased
- 2002-04-16 CA CA002443978A patent/CA2443978A1/en not_active Abandoned
- 2002-04-16 US US10/475,113 patent/US20040129567A1/en not_active Abandoned
- 2002-04-16 JP JP2002581976A patent/JP2004524540A/ja active Pending
- 2002-04-16 EP EP02720223A patent/EP1379864A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1379864A1 (en) | 2004-01-14 |
| JP2004524540A (ja) | 2004-08-12 |
| US20040129567A1 (en) | 2004-07-08 |
| WO2002084273A1 (en) | 2002-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |