CA2434469A1 - Appareil et procede d'electrophorese a film mince - Google Patents

Appareil et procede d'electrophorese a film mince Download PDF

Info

Publication number
CA2434469A1
CA2434469A1 CA002434469A CA2434469A CA2434469A1 CA 2434469 A1 CA2434469 A1 CA 2434469A1 CA 002434469 A CA002434469 A CA 002434469A CA 2434469 A CA2434469 A CA 2434469A CA 2434469 A1 CA2434469 A1 CA 2434469A1
Authority
CA
Canada
Prior art keywords
reservoir
axis
detecting
membrane
data points
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002434469A
Other languages
English (en)
Inventor
Andras Guttman
Bart Wanders
Phil Alei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Participations AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from US10/047,461 external-priority patent/US20030132117A1/en
Publication of CA2434469A1 publication Critical patent/CA2434469A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une électrophorèse à débit élevé dans laquelle une pluralité d'échantillons migrent à travers un milieu de séparation de très grande résistivité sous l'effet d'un champ électrique. De multiples passages d'électrophorèse sont exécutés au moyen d'un seul milieu de séparation. En utilisant un densitomètre optique ou d'autres systèmes de détection, des techniques automatiques d'analyse de données permettent d'obtenir des données quantitatives et qualitatives. Les températures de fonctionnement régulées et la géométrie réduite permettent d'atténuer l'imprécision de dispersion dans les données en bandes.
CA002434469A 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince Abandoned CA2434469A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US75998901A 2001-01-12 2001-01-12
US09/759,989 2001-01-12
US10/047,461 2002-01-14
PCT/US2002/000985 WO2002056004A2 (fr) 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince
US10/047,461 US20030132117A1 (en) 2002-01-14 2002-01-14 Thin film electrophoresis apparatus and method

Publications (1)

Publication Number Publication Date
CA2434469A1 true CA2434469A1 (fr) 2002-07-18

Family

ID=26725040

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002434469A Abandoned CA2434469A1 (fr) 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince

Country Status (3)

Country Link
AU (1) AU2002239908A1 (fr)
CA (1) CA2434469A1 (fr)
WO (2) WO2002056004A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2439233B (en) * 2003-09-22 2008-04-23 Shimadzu Corp An electrophoretic separation method
JP2007506092A (ja) * 2003-09-22 2007-03-15 株式会社島津製作所 電気泳動装置及び方法、並びに電気泳動部材及び試料分注プローブ
CN100447567C (zh) * 2004-09-22 2008-12-31 杭州生源医疗保健技术开发有限公司 构建组合离子膜微电色谱的方法
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
EP3677905A1 (fr) 2007-04-04 2020-07-08 ANDE Corporation Analyse d'acide nucléique intégrée
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
WO2010147654A2 (fr) 2009-06-15 2010-12-23 Netbio Inc. Procédés perfectionnés pour une quantification d'adn à des fins médicolégales
US8829426B2 (en) 2011-07-14 2014-09-09 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
GB2502953B (en) 2012-05-24 2017-02-08 Imp Innovations Ltd Catalytic converter substrate
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB2517951A (en) 2013-09-05 2015-03-11 Imp Innovations Ltd A substrate and a method of manufacturing a substrate
US10115577B1 (en) * 2017-09-07 2018-10-30 California Institute Of Technology Isotope ratio mass spectrometry

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588491A (en) * 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
US5137613A (en) * 1990-12-20 1992-08-11 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
FR2671290B1 (fr) * 1991-01-04 1993-04-16 Sebia Sa Dispositif pour l'application d'echantillons biologiques sur une plaque d'electrophorese.
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5990373A (en) * 1996-08-20 1999-11-23 Kansas State University Research Foundation Nanometer sized metal oxide particles for ambient temperature adsorption of toxic chemicals
US6057488A (en) * 1998-09-15 2000-05-02 Nantek, Inc. Nanoparticles for the destructive sorption of biological and chemical contaminants
WO2000065336A1 (fr) * 1999-04-26 2000-11-02 Mj Research, Inc. Ensemble d'electrophorese et procede de coulage de gels d'electrophorese
AU4592200A (en) * 1999-05-06 2000-11-21 University Of Southampton Gel electrophoresis

Also Published As

Publication number Publication date
WO2002055189A2 (fr) 2002-07-18
WO2002056004A8 (fr) 2003-01-16
WO2002055189A3 (fr) 2004-10-28
WO2002056004A3 (fr) 2004-02-19
WO2002056004A2 (fr) 2002-07-18
AU2002239908A1 (en) 2002-07-24

Similar Documents

Publication Publication Date Title
US5395502A (en) Apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems
CA2434469A1 (fr) Appareil et procede d'electrophorese a film mince
EP2669669B1 (fr) Unité capillaire pour appareil d'electrophorèse
JP4754759B2 (ja) 化合物の電気泳動分離
EP3226993B1 (fr) Appareil et procédé pour séparer des molécules
US20010023826A1 (en) Automated system for two dimensional electrophoresis
JP2003501639A (ja) 横断電気泳動および等電点電気泳動法のための微小流体デバイス
EP2062036A1 (fr) Procedes, cassettes, gels et appareils d'isolement et de collecte de biomolecules a partir de gels d'electrophorese
US5137613A (en) Horizontal gel electrophoresis apparatus
EP0397699B1 (fr) Appareil d'electrophorese capillaire automatique
Tsai et al. Application of plasma-polymerized films for isoelectric focusing of proteins in a capillary electrophoresis chip
JP3410099B2 (ja) 担体両性電解質を使用しない等電点電気泳動方法及び装置
US20040045829A1 (en) Cassette, system, and 2-D gel electrophoresis method for separating molecules
WO2002084273A1 (fr) Systeme de separation electrophoretique
US20030132117A1 (en) Thin film electrophoresis apparatus and method
EP1412733A2 (fr) Appareil et procede d'electrophorese a film mince
AU2002239906A1 (en) Thin film electrophoresis apparatus and method
JP4067441B2 (ja) キャピラリー電気泳動方法、キャピラリー電気泳動プログラム、そのプログラムを記憶した記録媒体及びキャピラリー電気泳動装置
KR20180109647A (ko) 단일 지점 검출 방식 미소유체 등전점 전기영동 및 미소유체 칩
JP4165674B2 (ja) 液中微粒子の電磁泳動分析法およびその装置
JPH0674937A (ja) 細管式電気泳動方法およびその装置並びにこれらに用いられるカラム
US5344534A (en) Gel electrophoresis measurement of dynamic reaction product by continuous sampling
WO1997033166A1 (fr) Procede d'approvisionnement en echantillons d'un appareil a electrophorese capillaire et dispositif correspondant
Hashimoto et al. Design of a pressure-mobilization system for capillary isoelectric focusing-chemiluminescence detection
Ossicini Electrophoretic methods: State of the art, trends and developments

Legal Events

Date Code Title Description
FZDE Discontinued