CA2433354A1 - Affinity selection-based screening of hydrophobic proteins - Google Patents

Affinity selection-based screening of hydrophobic proteins Download PDF

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Publication number
CA2433354A1
CA2433354A1 CA002433354A CA2433354A CA2433354A1 CA 2433354 A1 CA2433354 A1 CA 2433354A1 CA 002433354 A CA002433354 A CA 002433354A CA 2433354 A CA2433354 A CA 2433354A CA 2433354 A1 CA2433354 A1 CA 2433354A1
Authority
CA
Canada
Prior art keywords
protein
seq
tag
hydrophobic
cooh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002433354A
Other languages
English (en)
French (fr)
Inventor
Jason S. Felsch
David Allen Annis Jr.
Krishna Kalghatgi
Huw M. Nash
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neogenesis Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2433354A1 publication Critical patent/CA2433354A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99001—Prostaglandin-endoperoxide synthase (1.14.99.1), i.e. cyclooxygenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Neurology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA002433354A 2000-12-29 2001-12-19 Affinity selection-based screening of hydrophobic proteins Abandoned CA2433354A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25897000P 2000-12-29 2000-12-29
US60/258,970 2000-12-29
PCT/US2001/050088 WO2002057792A2 (en) 2000-12-29 2001-12-19 Affinity selection-based screening of hydrophobic proteins

Publications (1)

Publication Number Publication Date
CA2433354A1 true CA2433354A1 (en) 2002-07-25

Family

ID=22982920

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002433354A Abandoned CA2433354A1 (en) 2000-12-29 2001-12-19 Affinity selection-based screening of hydrophobic proteins

Country Status (5)

Country Link
US (1) US20020164617A1 (https=)
EP (1) EP1379878A2 (https=)
JP (1) JP2005506039A (https=)
CA (1) CA2433354A1 (https=)
WO (1) WO2002057792A2 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099774A2 (en) * 2003-05-07 2004-11-18 Cellzome Ag Processes for the identification of medical targets by screening an active agent for binding interactions
US20080097084A1 (en) * 2003-09-25 2008-04-24 Mendez Michael J Rho Antibody And Tag To Purify Cell Surface Proteins
CA2633790C (en) 2005-12-22 2016-08-09 Henry M. Krause Methods and compositions for the detection and isolation of ligands
US8468002B2 (en) * 2007-03-22 2013-06-18 Infosys Limited Annotating descriptions of chemical compounds
US9783585B2 (en) * 2012-09-10 2017-10-10 Johns Hopkins University Compositions and methods for increasing the expression and signalling of proteins on cell surfaces
WO2017209990A1 (en) * 2016-05-31 2017-12-07 Exxonmobil Upstream Research Company METHODS FOR lSOLATING NUCLEIC ACIDS FROM SAMPLES
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
CN115851686A (zh) * 2022-07-29 2023-03-28 复旦大学附属中山医院 Sirt6 h133y蛋白及其富集豆蔻酰化修饰肽段的方法和应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5543297A (en) * 1992-12-22 1996-08-06 Merck Frosst Canada, Inc. Human cyclooxygenase-2 cDNA and assays for evaluating cyclooxygenase-2 activity
WO1994021665A1 (en) * 1993-03-24 1994-09-29 Amylin Pharmaceuticals, Inc. Cloned receptors and methods for screening
WO1995002611A1 (en) * 1993-07-16 1995-01-26 Schering Corporation Cell surface protein present on nk (natural killer cells) named dx1
US6017768A (en) * 1994-05-06 2000-01-25 Pharmacopeia, Inc. Combinatorial dihydrobenzopyran library
US5962337A (en) * 1995-06-29 1999-10-05 Pharmacopeia, Inc. Combinatorial 1,4-benzodiazepin-2,5-dione library
EP0914608A1 (en) * 1996-05-09 1999-05-12 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
GB9610813D0 (en) * 1996-05-23 1996-07-31 Pharmacia Spa Combinatorial solid phase synthesis of a library of benzufuran derivatives
GB9610811D0 (en) * 1996-05-23 1996-07-31 Pharmacia Spa Combinatorial solid phase synthesis of a library of indole derivatives
US6025371A (en) * 1996-10-28 2000-02-15 Versicor, Inc. Solid phase and combinatorial library syntheses of fused 2,4-pyrimidinediones
US6114309A (en) * 1997-11-21 2000-09-05 Incara Research Laboratories Combinatorial library of moenomycin analogs and methods of producing same
US6207861B1 (en) * 1998-01-05 2001-03-27 Neogenesis, Inc. Method for producing and screening mass coded combinatorial libraries for drug discovery and target validation
DE69935905D1 (de) * 1998-01-15 2007-06-06 Whitehead Biomedical Inst Fettsäure transportproteine
JP2002517466A (ja) * 1998-06-08 2002-06-18 アドバンスド メディスン インコーポレーテッド 新規カリウムチャンネル薬剤およびそれらの用途
US6147344A (en) * 1998-10-15 2000-11-14 Neogenesis, Inc Method for identifying compounds in a chemical mixture

Also Published As

Publication number Publication date
WO2002057792A2 (en) 2002-07-25
JP2005506039A (ja) 2005-03-03
WO2002057792A3 (en) 2003-11-06
US20020164617A1 (en) 2002-11-07
EP1379878A2 (en) 2004-01-14

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Legal Events

Date Code Title Description
FZDE Discontinued