CA2431662A1 - A method for the manufacture of compositions containing low concentrations of salts - Google Patents
A method for the manufacture of compositions containing low concentrations of salts Download PDFInfo
- Publication number
- CA2431662A1 CA2431662A1 CA002431662A CA2431662A CA2431662A1 CA 2431662 A1 CA2431662 A1 CA 2431662A1 CA 002431662 A CA002431662 A CA 002431662A CA 2431662 A CA2431662 A CA 2431662A CA 2431662 A1 CA2431662 A1 CA 2431662A1
- Authority
- CA
- Canada
- Prior art keywords
- ligand
- ion
- substance
- liquid
- exchanger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 59
- 150000003839 salts Chemical class 0.000 title claims description 40
- 239000000203 mixture Substances 0.000 title claims description 13
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 239000003446 ligand Substances 0.000 claims abstract description 228
- 239000000126 substance Substances 0.000 claims abstract description 116
- 239000007788 liquid Substances 0.000 claims abstract description 75
- 239000011159 matrix material Substances 0.000 claims abstract description 46
- 238000011033 desalting Methods 0.000 claims abstract description 28
- 238000005342 ion exchange Methods 0.000 claims abstract description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 16
- 239000011780 sodium chloride Substances 0.000 claims abstract description 8
- 239000000872 buffer Substances 0.000 claims description 25
- 238000005341 cation exchange Methods 0.000 claims description 22
- 238000005349 anion exchange Methods 0.000 claims description 17
- 230000003139 buffering effect Effects 0.000 claims description 11
- 230000001419 dependent effect Effects 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 7
- 150000002500 ions Chemical class 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 102000015636 Oligopeptides Human genes 0.000 claims description 2
- 108010038807 Oligopeptides Proteins 0.000 claims description 2
- 125000003473 lipid group Chemical group 0.000 claims description 2
- 150000007523 nucleic acids Chemical group 0.000 claims description 2
- 239000002773 nucleotide Chemical group 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 210000004896 polypeptide structure Anatomy 0.000 claims description 2
- 125000002345 steroid group Chemical group 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 239000003125 aqueous solvent Substances 0.000 claims 1
- 125000000837 carbohydrate group Chemical group 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 238000001694 spray drying Methods 0.000 claims 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 52
- 229940098773 bovine serum albumin Drugs 0.000 description 52
- 238000011084 recovery Methods 0.000 description 51
- 125000004429 atom Chemical group 0.000 description 32
- 102000004169 proteins and genes Human genes 0.000 description 29
- 108090000623 proteins and genes Proteins 0.000 description 29
- 230000003993 interaction Effects 0.000 description 27
- 239000002585 base Substances 0.000 description 24
- 239000000047 product Substances 0.000 description 19
- 229920002684 Sepharose Polymers 0.000 description 17
- 238000001179 sorption measurement Methods 0.000 description 16
- -1 sulphopropyl groups Chemical group 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 125000003118 aryl group Chemical group 0.000 description 14
- 238000003795 desorption Methods 0.000 description 14
- 239000002253 acid Substances 0.000 description 13
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 11
- 230000002209 hydrophobic effect Effects 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 9
- 230000008878 coupling Effects 0.000 description 9
- 238000010168 coupling process Methods 0.000 description 9
- 238000005859 coupling reaction Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 150000001408 amides Chemical class 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 8
- 230000008859 change Effects 0.000 description 7
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- 125000006850 spacer group Chemical group 0.000 description 7
- 150000003568 thioethers Chemical class 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
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- 229910052757 nitrogen Inorganic materials 0.000 description 6
- VUUSTLFFRIFAIC-YFKPBYRVSA-N (2s)-3-hydroxy-2-(thiophen-2-ylamino)propanoic acid Chemical compound OC[C@@H](C(O)=O)NC1=CC=CS1 VUUSTLFFRIFAIC-YFKPBYRVSA-N 0.000 description 5
- 239000005864 Sulphur Substances 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 125000004432 carbon atom Chemical group C* 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 4
- 125000000217 alkyl group Chemical group 0.000 description 4
- 150000002430 hydrocarbons Chemical group 0.000 description 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- 239000012460 protein solution Substances 0.000 description 4
- 125000001453 quaternary ammonium group Chemical group 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- STMDPCBYJCIZOD-UHFFFAOYSA-N 2-(2,4-dinitroanilino)-4-methylpentanoic acid Chemical compound CC(C)CC(C(O)=O)NC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O STMDPCBYJCIZOD-UHFFFAOYSA-N 0.000 description 3
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 108091006522 Anion exchangers Proteins 0.000 description 3
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 125000003368 amide group Chemical group 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 3
- 229910052794 bromium Inorganic materials 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 150000001721 carbon Chemical group 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000006167 equilibration buffer Substances 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 3
- 125000001072 heteroaryl group Chemical group 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 229910001415 sodium ion Inorganic materials 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 125000001424 substituent group Chemical group 0.000 description 3
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical class C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 2
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical group C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 2
- 125000003342 alkenyl group Chemical group 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 125000000129 anionic group Chemical group 0.000 description 2
- 230000002902 bimodal effect Effects 0.000 description 2
- 235000010633 broth Nutrition 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 150000007942 carboxylates Chemical class 0.000 description 2
- 125000002091 cationic group Chemical group 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
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- 125000004122 cyclic group Chemical group 0.000 description 2
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- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical group C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
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- 229920001600 hydrophobic polymer Polymers 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
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- 229910052753 mercury Inorganic materials 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 125000004433 nitrogen atom Chemical group N* 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-M phenolate Chemical compound [O-]C1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-M 0.000 description 2
- 229940031826 phenolate Drugs 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 2
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
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- XFNJVJPLKCPIBV-UHFFFAOYSA-N trimethylenediamine Chemical compound NCCCN XFNJVJPLKCPIBV-UHFFFAOYSA-N 0.000 description 2
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- NFKMPKOGCRBAJF-UHFFFAOYSA-N $l^{1}-azanylformonitrile Chemical compound [N]C#N NFKMPKOGCRBAJF-UHFFFAOYSA-N 0.000 description 1
- KWZAXBZEPADBLS-UHFFFAOYSA-N 2-[2-[[[2-(4-bromothiophen-2-yl)-2-hydroxyethyl]amino]methyl]phenoxy]-n-ethylacetamide Chemical compound CCNC(=O)COC1=CC=CC=C1CNCC(O)C1=CC(Br)=CS1 KWZAXBZEPADBLS-UHFFFAOYSA-N 0.000 description 1
- 125000000175 2-thienyl group Chemical group S1C([*])=C([H])C([H])=C1[H] 0.000 description 1
- BWRRWBIBNBVHQF-UHFFFAOYSA-N 4-(3-pyridin-2-yl-1,2,4-oxadiazol-5-yl)butanoic acid Chemical compound O1C(CCCC(=O)O)=NC(C=2N=CC=CC=2)=N1 BWRRWBIBNBVHQF-UHFFFAOYSA-N 0.000 description 1
- ROMPPAWVATWIKR-UHFFFAOYSA-N 4-[3-(4-chlorophenyl)-1,2,4-oxadiazol-5-yl]butanoic acid Chemical compound O1C(CCCC(=O)O)=NC(C=2C=CC(Cl)=CC=2)=N1 ROMPPAWVATWIKR-UHFFFAOYSA-N 0.000 description 1
- NUKYPUAOHBNCPY-UHFFFAOYSA-N 4-aminopyridine Chemical compound NC1=CC=NC=C1 NUKYPUAOHBNCPY-UHFFFAOYSA-N 0.000 description 1
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- JGJLWPGRMCADHB-UHFFFAOYSA-N hypobromite Inorganic materials Br[O-] JGJLWPGRMCADHB-UHFFFAOYSA-N 0.000 description 1
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- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
- 239000012038 nucleophile Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- BPUBBGLMJRNUCC-UHFFFAOYSA-N oxygen(2-);tantalum(5+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[Ta+5].[Ta+5] BPUBBGLMJRNUCC-UHFFFAOYSA-N 0.000 description 1
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 description 1
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920002454 poly(glycidyl methacrylate) polymer Polymers 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical class CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 235000019423 pullulan Nutrition 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 125000006413 ring segment Chemical group 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 1
- 235000019254 sodium formate Nutrition 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 150000003440 styrenes Chemical class 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical class OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-O sulfonium Chemical compound [SH3+] RWSOTUBLDIXVET-UHFFFAOYSA-O 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 229910001936 tantalum oxide Inorganic materials 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 229930192474 thiophene Natural products 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- JSPLKZUTYZBBKA-UHFFFAOYSA-N trioxidane Chemical class OOO JSPLKZUTYZBBKA-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 150000003672 ureas Chemical class 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 229910001928 zirconium oxide Inorganic materials 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/04—Processes using organic exchangers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/26—Cation exchangers for chromatographic processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/04—Processes using organic exchangers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Water By Ion Exchange (AREA)
- Seasonings (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0004933A SE0004933D0 (sv) | 2000-07-17 | 2000-12-31 | A method for the manufacture of compositions containing low concentrations of salt |
| SE0004933-8 | 2000-12-31 | ||
| PCT/EP2001/015015 WO2002053288A2 (en) | 2000-12-31 | 2001-12-19 | A method for the manufacture of compositions containing low concentrations of salts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2431662A1 true CA2431662A1 (en) | 2002-07-11 |
Family
ID=20282508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002431662A Abandoned CA2431662A1 (en) | 2000-12-31 | 2001-12-19 | A method for the manufacture of compositions containing low concentrations of salts |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6852230B2 (https=) |
| EP (1) | EP1345694B1 (https=) |
| JP (1) | JP2004532182A (https=) |
| AT (1) | ATE334746T1 (https=) |
| AU (1) | AU2002235795A1 (https=) |
| CA (1) | CA2431662A1 (https=) |
| DE (1) | DE60121999T2 (https=) |
| ES (1) | ES2267840T3 (https=) |
| WO (1) | WO2002053288A2 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0300612D0 (sv) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing ligands for hydrophobic interaction chromatography |
| SE0300624D0 (sv) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing affinity ligands |
| DE602004018616D1 (de) * | 2003-03-05 | 2009-02-05 | Ge Healthcare Bio Sciences Ab | Verfahren zur herstellung von multimodalen anionenaustauschliganden |
| US7045059B2 (en) * | 2003-07-28 | 2006-05-16 | Sielc Technologies Corp | Universal bonded phase material for chromatographic separation |
| SE0400501D0 (sv) * | 2004-02-27 | 2004-02-27 | Amersham Biosciences Ab | Antibody purification |
| KR101150050B1 (ko) * | 2004-02-27 | 2012-07-05 | 지이 헬스케어 바이오-사이언시스 에이비 | 항체 정제 방법 |
| SE529259C2 (sv) * | 2005-08-31 | 2007-06-12 | Ge Healthcare Bio Sciences Ab | Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi |
| CN101678318B (zh) * | 2007-05-25 | 2013-09-25 | 默克专利股份公司 | 用于阳离子交换色谱法的接枝共聚物 |
| WO2010005364A1 (en) | 2008-07-08 | 2010-01-14 | Ge Healthcare Bio-Sciences Ab | Separation medium for chromatography of various biomolecules |
| US20100029371A1 (en) * | 2008-08-01 | 2010-02-04 | Gennady Medvinsky | Personal Game Services Commerce System (PGSCS) |
| EP2488295A1 (en) * | 2009-10-12 | 2012-08-22 | GE Healthcare Bio-Sciences AB | Separation matrices |
| MX341036B (es) | 2009-12-07 | 2016-08-04 | Fresenius Medical Care Holdings Inc | Cartucho para purificacion de agua que usa sorbentes de intercambio ionico de zirconio. |
| IN2012DN06243A (https=) * | 2010-02-19 | 2015-09-25 | Ge Healthcare Bio Sciences Ab | |
| JP6420817B2 (ja) * | 2013-03-15 | 2018-11-07 | バイオコンパティブルズ ユーケー リミテッド | 結像可能な塞栓性微小球 |
| DE102016004432A1 (de) | 2016-04-12 | 2017-10-12 | Sartorius Stedim Biotech Gmbh | Multimodales Adsorptionsmedium mit multimodalen Liganden, Verfahren zu dessen Herstellung und dessen Verwendung |
| JP6910033B2 (ja) | 2016-08-01 | 2021-07-28 | 株式会社カネカ | カルシプロテインパーティクルの吸着材、および吸着除去システムとその利用方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3234199A (en) * | 1958-12-18 | 1966-02-08 | Allen F Reid | Ion exchange process for separating proteins |
| JPS5927830A (ja) * | 1982-08-09 | 1984-02-14 | Cosmo Co Ltd | 抗腫瘍性糖蛋白質物質 |
| US4599197A (en) * | 1982-12-22 | 1986-07-08 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| DK114392D0 (da) * | 1992-09-17 | 1992-09-17 | Kem En Tec As | Isolering af biomolekyler |
| US5652348A (en) * | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| GB9707696D0 (en) * | 1997-04-16 | 1997-06-04 | Agner Erik | Improvements in or relating to chromatography |
| SE9802214D0 (sv) | 1998-06-18 | 1998-06-18 | Amersham Pharm Biotech Ab | Jonbytare och användning därav |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| SE9904197D0 (sv) | 1999-11-22 | 1999-11-22 | Amersham Pharm Biotech Ab | A method for anion exchange adsorption on matrices carrying mixed mode ligands |
| SE0002688D0 (sv) | 2000-07-17 | 2000-07-17 | Amersham Pharm Biotech Ab | Adsorption method and ligands |
-
2001
- 2001-12-19 WO PCT/EP2001/015015 patent/WO2002053288A2/en not_active Ceased
- 2001-12-19 EP EP01985906A patent/EP1345694B1/en not_active Expired - Lifetime
- 2001-12-19 AU AU2002235795A patent/AU2002235795A1/en not_active Abandoned
- 2001-12-19 AT AT01985906T patent/ATE334746T1/de not_active IP Right Cessation
- 2001-12-19 CA CA002431662A patent/CA2431662A1/en not_active Abandoned
- 2001-12-19 DE DE60121999T patent/DE60121999T2/de not_active Expired - Lifetime
- 2001-12-19 JP JP2002554232A patent/JP2004532182A/ja active Pending
- 2001-12-19 US US10/451,195 patent/US6852230B2/en not_active Expired - Fee Related
- 2001-12-19 ES ES01985906T patent/ES2267840T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ATE334746T1 (de) | 2006-08-15 |
| WO2002053288A2 (en) | 2002-07-11 |
| US6852230B2 (en) | 2005-02-08 |
| DE60121999D1 (de) | 2006-09-14 |
| US20040050784A1 (en) | 2004-03-18 |
| DE60121999T2 (de) | 2007-07-26 |
| JP2004532182A (ja) | 2004-10-21 |
| EP1345694B1 (en) | 2006-08-02 |
| EP1345694A2 (en) | 2003-09-24 |
| WO2002053288A3 (en) | 2002-12-12 |
| ES2267840T3 (es) | 2007-03-16 |
| AU2002235795A1 (en) | 2002-07-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |