CA2426035A1 - Peptides de liaison a la streptavidine et leurs utilisations - Google Patents

Peptides de liaison a la streptavidine et leurs utilisations Download PDF

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Publication number
CA2426035A1
CA2426035A1 CA002426035A CA2426035A CA2426035A1 CA 2426035 A1 CA2426035 A1 CA 2426035A1 CA 002426035 A CA002426035 A CA 002426035A CA 2426035 A CA2426035 A CA 2426035A CA 2426035 A1 CA2426035 A1 CA 2426035A1
Authority
CA
Canada
Prior art keywords
peptide
streptavidin
fusion protein
protein
dissociation constant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002426035A
Other languages
English (en)
Inventor
Jack W. Szostak
David S. Wilson, Jr.
Anthony D. Keefe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2426035A1 publication Critical patent/CA2426035A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur des peptides présentant une grande affinité pour la streptavidine. Ces peptides peuvent être exprimés en tant qu'éléments de protéines hybrides pouvant faciliter la détection, le dosage et la purification de protéines d'intérêt.
CA002426035A 2000-10-31 2000-10-31 Peptides de liaison a la streptavidine et leurs utilisations Abandoned CA2426035A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/041717 WO2002038580A1 (fr) 2000-10-31 2000-10-31 Peptides de liaison a la streptavidine et leurs utilisations

Publications (1)

Publication Number Publication Date
CA2426035A1 true CA2426035A1 (fr) 2002-05-16

Family

ID=21742173

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002426035A Abandoned CA2426035A1 (fr) 2000-10-31 2000-10-31 Peptides de liaison a la streptavidine et leurs utilisations

Country Status (4)

Country Link
EP (1) EP1337543A4 (fr)
JP (1) JP2004537965A (fr)
CA (1) CA2426035A1 (fr)
WO (1) WO2002038580A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10113776B4 (de) 2001-03-21 2012-08-09 "Iba Gmbh" Isoliertes streptavidinbindendes, kompetitiv eluierbares Peptid, dieses umfassendes Fusionspeptid, dafür codierende Nukleinsäure, Expressionsvektor, Verfahren zur Herstellung eines rekombinanten Fusionsproteins und Verfahren zum Nachweis und/oder zur Gewinnung des Fusionsproteins
JP2005536184A (ja) * 2002-03-01 2005-12-02 エルドマン，ヴォルカー，エー． ストレプトアビジン結合ペプチド
JP5691278B2 (ja) * 2010-07-26 2015-04-01 パナソニック株式会社 蛋白質固定化用アンカーペプチドを提供する方法
CN103267841B (zh) * 2013-05-15 2014-03-12 湖南农业大学 一种利用亲和素结合肽与亲和素结合的原理制备elisa酶标抗原的方法
WO2023222890A1 (fr) 2022-05-20 2023-11-23 Ciloa Chargement réversible de protéines dans la lumière de vésicules extracellulaires

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4237113B4 (de) * 1992-11-03 2006-10-12 "Iba Gmbh" Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins
DE19641876B4 (de) * 1996-10-10 2011-09-29 Iba Gmbh Streptavidinmuteine

Also Published As

Publication number Publication date
EP1337543A1 (fr) 2003-08-27
EP1337543A4 (fr) 2004-11-17
JP2004537965A (ja) 2004-12-24
WO2002038580A1 (fr) 2002-05-16

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Legal Events

Date Code Title Description
FZDE Discontinued