CA2410284A1 - Nucleotide sequence of influenza a/udorn/72 (h3n2) genome - Google Patents

Abstract

The complete nucleotide sequence of each of the eight segments of the negative strand Influenza A virus strain A/Udorn/72 (H3N2) is disclosed, which provides the complete sequence of the genome in antigenomic message sense. The sequence is useful in diagnosis and may be modified by mutation to generate new influenza A variant strains.

Description

NUCELOTIDE SEQUENCE OF
INFLUENZA A/Udorn/72 (H3N2) GENOME
Field Of The Invention This invention relates to the nucleotide sequence of each of the segments of the Influenza virus strain A/Udorn/72 (H3N2).
Background Of The Invention The influenza viruses consist of subtypes designated A, B and C. Influenza A viruses possess a single negative strand RNA genome of eight segments, which encodes 10 polypeptides (proteins) that are required for the life cycle of the virus. The order of the proteins is as follows:
Segment: 1 2 3 4 5 6 7 8 Protein: PB2 PB1 PA HA NP NA M1 NS1 Spliced M2 NS2 Product:
Each of the eight RNA,segments of the influenza subtype A complete genome is encapsidated with multiple subunits of the nucleocapsid protein (NP) and associated with a few molecules of the trimeric polymerase (PB1, PB2 and PA subunits), thereby forming the ribonucleoprotein complex (RNP) (Lamb, R.A., pages 1-87 of The Influenza Viruses, R.M.
Krug, ed. (Plenum Press, 1989)). The NP protein is a structural and transcription/replication regulatory protein. Surrounding these structures is a.layer of the matrix protein (M1), which is the major structural component of the virion and appears to serve as a nexus between the core and the viral envelope. This host cell-derived envelope is studded with the two _ 2 _ major virally encoded surface glycoproteins hemagglutinin (HA) and neuraminidase (NA), which are antigenic determinants, and a much smaller amount of a nonglycosylated small protein M2 (Lamb 1989; Lamb, R.A., et al., Cell, 40, 627-633 (1985)). The M2 protein is a spliced gene product from segment #7 (which also encodes the M1 protein) which has ion channel activity; spliced genes are only present in infected cells. Similarly, the nonstructural NS2 protein is a spliced gene product from segment #8 (which also encodes the nonstructural NS1 protein);
spliced genes are only present in infected cells. The HA glycoprotein is cleaved by a protease to form HAl and HA2.
Influenza viral infection is initiated by the attachment of the surface hemagglutinin to a sialic acid-containing cellular receptor. This first virus-cell interaction induces the uptake of the viral particle into the cell by receptor-mediated endocytosis. Under the low pH conditions of the endosome, the HA undergoes a conformational change that facilitates the interaction of the hydrophobic NH2 terminal domain of HA2 and the endosomal membrane, resulting in membrane fusion and subsequent release of the core RNPs and matrix protein (M1) into the cytosol. Disassociation of the RNPs and matrix proteins occurs in the cytosol before the RNPs are translocated to the nucleus where transcription and replication of the complete genome take place (Martin, K., and Helenius, A., Cell, 67, 117-130 (1991);
Shapiro, G.I., et al., J. Virology, 61, 764-773 (1987) ) .
Following primary transcription, newly synthesized proteins initiate the replication of the viral genome which in turn increases transcription and protein synthesis. At this point of the virus life cycle, the surface glycoproteins HA and NA start to accumulate at discrete areas of the plasma membrane from where newly assembled virus will be released.
Virus assembly is assumed to begin via some sort of interaction between the cytoplasmic and/or transmembrane domains of the four virally encoded structural proteins: the membrane anchored proteins (HA, NA and M2), and the underlying matrix protein (M1), which in turn maintains a close association with the RNPs (Garoff, H., et al., Microbiology and Molecular Biology Reviews, 62, 1171-1190 (1998);
Nayak, D.P., ASM News, 62, 411-414 (1996)). The contacts between matrix protein M1 and the RNP
complexes, as well as the mechanism by which a complete set of eight RNPs gets incorporated into the mature virion particle, have not been well defined.
Specific molecular contacts among the structural components are assumed to dictate how the process of morphogenesis initiates and progresses to the point of mature particle assembly and budding from the surface of the host cell.
There are a large number of influenza A
virus subtypes and antigenic variants' that continuously evolve by mutations introduced by the RNA
polymerase during the replication of the viral genome.
Mutations also occur by genetic reassortment between the segments of two different subtypes when they happen to infect the same cellular host. This biological characteristic of influenza A virus gives rise to new virus strains, which necessitates periodic updating of the immunogenic compositions against influenza. Therefore, the availability of a complete nucleotide sequence and a full length clone of one particular strain of influenza A virus is essential for the development of new and innovative immunogenic compositions against influenza. Thus, there a.s a need to determine the complete nucleotide sequence of an influenza A virus and to construct a full length clone containing that sequence.
In particular. the influenza A strain designated Influenza A/Udorn/72 (H3N2) was first isolated in 1972. The nucleotide sequences of the polymerase genes PB2, PBl and PA have not been sequenced. The nucleotide sequences of the segments encoding HA, NP, M1, M2, NS1 and NS2 have been sequenced. However, those sequences were obtained a number of years ago using instrumentation which may not have provided accurate sequences. Thus, there is a need to repeat the sequencing of those segments in order to provide updated, accurate sequences.
Summary Of The Invention It is an object of this invention to determine the complete nucleotide sequence of the Influenza A/Udorn/72 (H3N2) viral strain and the spliced products of the segment #7 and #8 mRNAs. It is a~further object of this invention to generate a clone of each of the eight genes and spliced messages.
It is yet another object of this invention to determine the deduced amino acid sequences of all ten of the products of these genes and spliced messages.
These and other objects of this invention are achieved by the elucidation of isolated nucleic acid molecules constituting the nucleotide sequences of all of the segments of the Influenza A/Udorn/72 (H3N2) strain which together comprise the complete nucleotide sequence of that strain, as well as their spliced messages. All of these nucleotide sequences are presented in positive strand, antigenomic message sense.

In particular, the complete nucleotide sequence consists of SEQ ID N0:1, SEQ ID N0:3, SEQ ID
N0:5, SEQ ID N0:7, SEQ ID N0:9, SEQ ID N0:11, SEQ ID
N0:13 and SEQ ID N0:17, and biological equivalents thereof .
Alternatively, the complete nucleotide sequence includes a variant of the HA sequence, designated HA (P1) (SEQ ID N0:21), and consists of SEQ
ID N0:1, SEQ ID N0:3, SEQ ID N0:5, SEQ ID N0:9, SEQ ID
N0:11, SEQ ID N0:13, SEQ ID N0:17 and SEQ ID N0:21, and biological equivalents thereof.
In another embodiment of this invention, these isolated nucleic acid molecules are directed to individual isolated influenza A virus nucleic acid molecules which encode individual proteins and are selected from the group consisting of SEQ ID N0:1, SEQ
ID N0:3, SEQ ID N0:5, SEQ ID N0:7, SEQ ID N0:11 and SEQ ID N0:21 (the variant of SEQ ID N0:7), and biological equivalents thereof.
In yet another embodiment of this invention, these individual isolated nucleic acid molecules encode proteins having amino acid sequences selected from the group consisting of SEQ ID N0:2, SEQ ID N0:4 and SEQ ID N0:6, and biological equivalents thereof.
In a further embodiment of this invention, there are provided individual isolated influenza A
virus amino acid sequences, where said sequences are selected from the group consisting of SEQ ID N0:2, SEQ
ID N0:4 and SEQ ID N0:6, and biological equivalents thereof.
In a still further embodiment of this invention, there is provided an individual influenza A
virus amino acid sequence, where said sequence has the sequence of SEQ ID N0:8 or SEQ ID N0:12.
In still another embodiment of this invention, an isolated nucleic acid molecule having the nucleotide sequence of a segment of the Influenza A/Udorn/72 (H3N2) strain is used: (1) to design polymerase chain reaction (PCR) primers for use in a PCR assay to detect. the presence of the corresponding virus segment in a sample; or (2) to design and select peptides for use in an ELISA to detect the presence of the corresponding protein produced by that segment in a sample.
Detailed Description of the Invention The nucleotide sequences and deduced amino acid sequences of several of the segments of the Influenza A/Udorn/72 (H3N2) strain have been published. Yuferov et al. reported the sequence of the HA gene of segment 4 (Yuferov, V.P., et al., Proceedings of the Academy of Sciences of the USSR, 278, 738-742 (1984)). Buckler-White et al. reported the sequence of the NP gene of segment 5 (Buckler-White, A.J., and Murphy, B.R., Virology, 155, 345-355 (1986)). Markoff et al. reported the sequence of the NA gene of segment 6 (Markoff, L., and Lai, C.J., Virology, 119, 288-297 (1982)). Lamb et al. in 1981 reported the sequence of the' M1 gene and the M2 gene splice product of segment 7 (M1 in Lamb. R.A., and Lai, C.J., Virology, 112, 746-751 (1981)("Lamb Virology 1981"), and M2 in Lamb, R.A., et al., Proc.
Natl. Acad. Sci. USA, 78, 4170-4174 (1981)("Lamb PNAS
1981")). Lamb et al. in 1980 reported the sequence of the NS1 gene and the NS2 gene splice product of segment 8 (Lamb, R.A., and Lai, C.J., Cell, 21, 475-485 (1980) In order to resequence these segments, as well as to sequence those segments not previously elucidated, the Tnfluenza A/Udorn/72 (H3N2) strain was grown in and purified from Madin-Darby canine kidney _ 7 _ (MDCK) cells. The viral RNA was extracted from purified virions and amplified by RT-PCR using termini-specific primers. Gel purified genes were cloned into pGemT vectors (Promega) and sequenced using multiple sets of primers. The sequences of the termini were determined by 3' and 5' ligation and sequencing of the RT-PCR fragments crossing the.
junction (Galarza, J.M., et al., J. Virol., 70, 2360-2368 (1996)). The M2 and NS2 genes are spliced products of the mRNA of segments seven and eight, respectively. These genes were recovered from mRNA
purified from MDCK-Influenza A/Udorn-infected cells by RT-PCR using oligo-dT and gene-specific primers. The RT-PCR products were gel purified and cloned into pGemT vectors. All gene sequences were determined by using a fluorescence dye terminator with AmpliTaq DNA
polymerase (Perkin-Elmer) and an Applied Biosystems ABI 377 DNA sequencer. This procedure was repeated with multiple clones for each fragment; the sequences obtained were consistent.
The complete genome of the Influenza A/Udorn/72 (H3N2) strain totaled 13628 nucleotides.
This is the first description of the complete nucleotide sequence of the Influenza A/Udorn/72 (H3N2) strain.
The eight segments were sequenced in their coding regions, and their 5' and 3' non-coding regions, including regulatory sequences such as promoters, enhancers and polyadenylation signals. The segments, their number of nucleotides, their isolated nucleic acid molecule sequences (shown in positive strand, antigenomic, message sense, that is, in 5' to 3' orientation), their coding regions and amino acid translations are as follows:

_ g _ Segment #1: 2341 nucleotides, SEQ ID N0:1, coding region nucleotides 28-2304, encodes PB2, 759 amino acids (SEQ ID N0:2).
Segment #2: 2341 nucleotides, SEQ ID N0:3, coding region nucleotides 25-2295, encodes PB1, 757 amino acids (SEQ ID N0:4).
Segment #3: 2233 nucleotides, SEQ ID N0:5, coding region nucleotides 25-2172, encodes PA, 716 amino acids (SEQ ID N0:6).
Segment #4: 1765 nucleotides, SEQ ID N0:7, coding region nucleotides 30-1727, encodes HA, 566 amino acids (SEQ ID N0:8).
SEQ ID N0:7 and SEQ ID N0:8 differ from the published Yuferov et al. nucleotide and amino acid sequences for HA in several respects.
Yuferov SEQ ID NO: 7 or 8 Codon # Amino Acid # Codon # Amino Acid #
GAC Asp AAC Asn GGT Gly GGG Gly TAC Tyr TTC Phe GAC Asp AAC Asn _ g _ Segment #5: 1565 nucleotides, SEQ ID N0:9, coding region nucleotides 46-1539, encodes NP, 498 amino acids (SEQ ID N0:10).
SEQ ID N0:9 and SEQ ID NO:10 are identical to the published Buckler-White et al. nucleotide and amino acid sequences for NP.
Segment #6: 1466 nucleotides, SEQ ID N0:11, coding region nucleotides 20-1426, encodes NA, 469 amino acids (SEQ ID N0:12).
SEQ ID N0:11 and SEQ ID N0:12 differ from the published Markoff et al. nucleotide and amino acid sequences for NA in several respects.
Markoff SEQ ID NO: 11 or 12 Codon # Amino Acid # Codon # Amino Acid #
CAG Gln CTG Leu GGA Gly GCA Ala CAA Gln CAG Gln ACA Thr GCA Ala There are also several differences in the leader sequence upstream of the methionine at amino acid 1.
Segment #7: 1027 nucleotides, SEQ ID N0:13, coding region nucleotides 26-781, encodes M1, 252 amino acids (SEQ ID N0:14).

SEQ ID N0:13 and SEQ ID NO:14 are identical to the published Lamb Virology 1981 nucleotide and amino acid sequences for M1.
Segment #7, spliced product: 322 nucleotides, SEQ ID
N0:15, coding region nucleotides 26-316, encodes M2, 97 amino acids (SEQ ID N0:16) [first spliced amino acid at residue 10, nucleotides 53-55].
SEQ ID N0:15 and SEQ ID N0:16 are identical to the published Lamb PNAS 1981 nucleotide and amino acid sequences for M2.
Segment #8: 890 nucleotides, SEQ ID N0:17, coding region nucleotides 27-737, encodes NS1, 237 amino acids (SEQ TD NO: 18).
SEQ ID N0:17 and SEQ ID N0:18 are identical , to the published Lamb et a1. 1980 nucleotide and amino acid sequences for NS1.
Segment #8, spliced product: 402 nucleotides, SEQ ID
N0:19, coding region nucleotides 27-389, encodes NS2, 121 amino acids (SEQ ID N0:20) [first spliced amino acid at residue 11, nucleotides 57-59].
SEQ ID N0:19 and SEQ ID N0:20 are identical to the published Lamb et al. 1980 nucleotide and amino acid sequences for NS2.
As mentioned above, the is also an alternate HA sequence, designated HA (P1):
Segment #4: 1764 nucleotides, SEQ ID N0:21, coding region nucleotides 30-1727, encodes HA, 566 amino acids (SEQ ID N0:22).
Without being bound by theory, the HA (P1) sequence may incorporate minor mutations from the Influenza A/Udorn/72 (H3N2) strain which occurred over time. The HA (P1) sequence (SEQ ID N0:21) has 1764 nucleotides, which is one fewer than in the HA
sequence (SEQ ID N0:7), due to a one nucleotide deletion in the non,-coding region of segment #4 (position 1756) .
The two HA sequences of this invention differ a.n their nucleotide and amino acid sequences as follows:
Nucleotide _HA HA (P1) Amino Acid Position Change 35 G A Silent 81 A G Asn to Asp 1103 G T Silent 1486 T A Phe to Tyr 1614 A G Asn to Asp 1756 T deletion Outside coding region The HA (P1) sequence also differs slightly from the Yuferov et al. HA nucleotide sequence as follows:
Nucleotide HA (P1) Yuferov Amino Acid PO S 3. tl.On Change 35 A G Silent 1756 deletion T Outside coding region The nucleic acid sequences of the mRNAs of the segments, which are in genome, negative sense (that is, in 3' to 5' orientation), are the complements of the positive strand, antigenomic, message sense sequences set forth above.
In addition to the nucleotide sequences which encode the Influenza A/Udorn/72 (H3N2) viral proteins set forth above, the present invention further comprises isolated nucleic acid molecules containing individual influenza A virus nucleotide sequences which, by virtue of"the redundancy of the genetic code, are biologically equivalent to those sequences which encode the viral proteins, that is, these other nucleotide sequences are characterized by nucleotide sequences which differ from those set forth herein, but which encode a protein having the same amino acid sequence as that encoded by any of the nucleotide sequences in SEQ ID~N0:1, SEQ ID N0:3, SEQ
ID N0:5, SEQ ID N0:7, SEQ ID N0:11 and SEQ ID N0:21.
In particular, the invention contemplates those nucleotide sequences which are sufficiently duplicative of any of the sequences of SEQ ID N0:1, SEQ ID N0:3, SEQ ID N0:5, SEQ ID N0:7, SEQ ID N0:11 and SEQ ID NO:21, so as to permit hybridization therewith under standard high stringency Southern hybridization conditions, such as those described in Sambrook et al. (Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
(1989) ) .
This invention also comprises nucleotide sequences which encode amino acid sequences which differ from those of the Influenza A/Udorn/72 (H3N2) viral proteins, but which are biologically equivalent to those described for one of these viral proteins (SEQ ID N0:2, SEQ ID N0:4 and SEQ ID N0:6). Such amino acid sequences may be said to be biologically equivalent to any of the viral proteins if their sequences differ only by minor deletions from, insertions into or substitutions to the viral protein sequences, such that the tertiary configurations of the sequences are essentially unchanged from those of the viral proteins.

For example, a codon for the amino acid alanine, a hydrophobic amino acid, may be substituted by a codon encoding another less hydrophobic residue, such as glycine, or a more hydrophobic residue, such as valine, leucine, or isoleucine. Similarly, changes which result in substitution of one negatively charged (acidic) residue for another, such as aspartic acid for glutamic acid, or one positively charged (basic) residue for another, such as lysine for arginine or histidine, as well as changes based on similarities of residues in their hydropathic index, can also be expected to produce a biologically equivalent product.
Nucleotide changes which result in alteration of the N-terminal or C-terminal portions of the protein molecule would also not be expected to alter the activity of the protein.
Each of the proposed modifications is well within the routine skill in the art, as is determi-nation of retention of structural and biological activity of the encoded products.
This invention further comprises the isolated amino acid sequences comprising the individual influenza A virus amino acid sequences selected from the group consisting of SEQ ID N0:2, SEQ
ID N0:4 and SEQ ID N0:6.
This invention still further comprises the , individual isolated amino acid sequences of,SEQ ID
N0:8 and SEQ ID N0:12.
The fundamental biological properties of the Influenza A/Udorn/72 (H3N2) virus depend on its genome, which consists of eight segments. The segments of influenza virus consists of a single strand of negative polarity RNA, and each viral strain has its own specific nucleotide sequence.
Complete differential identification between viral strains is, therefore, necessary to determine the nucleotide sequence of the virus. Since the entire specific nucleotide sequence, consisting of 13,628 bases, has been clearly determined as described herein, the virus can be identified at the genetic level, and the identification technique can therefore provide an absolute determination.
The sequences described herein are useful to provide an identification method comprising detecting a part of the nucleotide sequence of influenza viral antigenomic DNA using the polymerase chain reaction (PCR) method, as well as to generate peptides to detect an antigen produced by that DNA using an ELISA.
Having a completely sequenced and cloned genome of one particular influenza strain is advantageous for the research and development of new and innovative immunogenic compositions based on this genetic blueprint.
Defined mutations are introduced where desired into one or more of the segments whose sequences are set forth herein. One or more mutations are introduced by using site-directed mutagenesis.
However, a mutant influenza virus cannot be directly generated from viral RNA, because neither the genomic viral RNA nor the antigenomic cDNA can serve as a direct template for synthesis. Instead, the viral RNA, after its encapsidation by NP, must be transcribed into positive-sense mRNA by the viral RNA
polymerase complex.
Palese et al. (U. S. Patent 5,166,057, which is hereby incorporated by reference) described a reverse genetics helper virus-dependent system for the "rescue" of an influenza A virus segment. Briefly, a ribonucleoprotein (RNP) complex is generated by in vitro synthesis in the presence of the three polymerase proteins and NP. The RNP complex is then used to transfect eukaryotic cells. Subsequent infection with influenza A helper virus results in the generation of viruses possessing a gene derived from the cloned cDNA segment. A selection method is then used to separate the desired transfectants from the larger number of helper viruses.
A different system is used if an entire mutant influenza A strain is to be rescued. Using the system described by Neumann et al. (Neumann, G., et al., Proc. Natl. Acad. Sci. USA, 96, 9345-9350 (1999), which is hereby incorporated by reference), a modified Influenza virus strain A/Udorn/72 (H3N2) is generated entirely from cloned cDNAs. This plasmid-based system does not require the use of helper virus infection.
In summary, a cell line, such as human embryonic kidney cell line 293T, is transfected with eight plasmids, each encoding a viral RNA segment of the strain and flanked by a suitable RNA polymerase promoter and terminator, together with another four plasmids encoding the viral NP, PB2, PB1 and PA
proteins (which serve to synthesize RNPs intracellularly in vivo). Yields are increased substantially by the addition of five further plasmids expressing the viral structural proteins HA, NA, M1, M2 and NS2.
In a variation of this system, only eight plasmids are required (Hoffmann, E., et al., Proc.
Natl. Acad. Sci. USA, 97, 6108-6113 (2000), which is hereby incorporated by reference). Each plasmid contains two promoters, a human RNA polymerase I (pol I) promoter and a human RNA polymerase II (pol II) promoter. After transfection of eukaryotic cells with the eight expression plasmids, the human pol I and II
promoters each transcribe the plasmid template. This results in the synthesis of both viral mRNAs and vRNAs, ultimately leading to the generation of infectious influenza A virus.

Because helper virus is not required in any of the 8, 12 or 17 plasmid transfection systems, the transfectant viruses are recovered without plaque purification. This system facilitates the production of live attenuated influenza A viruses for use in combating epidemics involving new HA or NA subtypes, where the corresponding segment of the Influenza A/Udorn/72 (H3N2) strain is mutated to match the sequence of the new subtypes.
Predetermined mutations to the segment encoding HA or NA in the Influenza A/Udorn/72 (H3N2) strain which correspond to. the sequence of a new circulating subtype are made by using site-directed mutagenesis. Such mutations are introduced by standard recombinant DNA methods into a DNA copy of the viral genome.
If desired, reassortant viruses are generated by substituting for the segment encoding the HA or NA of the Influenza A/Udorn/72 (H3N2) strain (or mutant thereof) the corresponding segment from a different influenza virus strain.
In another embodiment of this invention, an isolated nucleic acid molecule having the nucleotide sequence of a segment of the Influenza A/Udorn/72 (H3N2) strain is used to generate oligonucleotide probes (from either positive strand antigenomic message sense or negative strand complementary genomic sense) and to express peptides (from positive strand antigenomic message sense only), which are used to detect the presence of a segment of that influenza A
strain (or a mutant thereof) in samples of body fluids and tissues. The nucleotide sequences are used to design.highly specific and sensitive diagnostic tests to detect the presence of the viral segment in a sample.

PCR primers are synthesized with sequences based on the viral sequences described herein. The test sample is subjected to reverse transcription of RNA, followed by PCR amplification, of selected cDNA
regions corresponding to the nucleotide sequence described herein which have nucleotides which are distinct for a defined segment of that viral strain.
Amplified PCR products are identified on gels and their specificity confirmed by hybridization with specific nucleotide probes.
ELISA tests are~used to detect the presence of a protein produced by a viral segment. Peptides are designed and selected to contain one or more distinct residues based on the viral sequences described herein. These peptides are then coupled to a hapten (e.g., keyhole limpet hemocyanin (KLH) and used to immunize animals (e.g., rabbits) for the production of monospecific polyclonal antibody. A
selection of these polyclonal antibodies, or a combination of polyclonal and monoclonal antibodies can then be used in a "capture ELISA" to detect a protein produced by that viral segment.
All references cited herein are hereby incorporated by reference.
In order that this invention may be better understood, the following examples are set forth. The examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention.

Examples Example 1 Sequencing of the Influenza A/Udorn/72 (H3N2) Strain The sequencing of each of the eight segments was carried out as follows:
RT-PCR Protocol Influenza A/Udorn/72 (H3N2) was grown in Madin-Darby canine kidney (MDCK) cells and concentrated from clarified culture supernatant.
Genomic viral RNA (vRNA) was prepared from 100,u1 of virus stock using a RNAeasy extraction kit (Qiagen).
The spliced products of mRNAs of segment numbers 7 and 8 (M2 and NS2 respectively) were. isolated from total mRNA of influenza/Udorn-infected MDCK cells using an Oligotex mRNA kit (Qiagen). Approximately l~,g of either vRNA or mRNA was added to an eppendorf tube containing 2pmol of gene-specific primer and diethyl pyrocarbonate-treated water (to inhibit RNAses) to a final volume of 12~C1. Oligo-dT primers were used to reverse transcribe the M2 and NS2 spliced messages.
The RNA-primer mixture was heated at 70°C for 10 minutes and quickly chilled on ice. The following reagents were added to this tube: 4~C1 of 5X first strand buffer (250mM Tris-HC1 pH 8.3, 375mM KC1, lSmM
MgClz), 2~1 of 0.1M DTT, and 1~C1 of dNTP mix (10 mM
each dATP, dTTP, dGTP, dCTP) and incubated at 42°C for two minutes. Subsequently, 1~,1 of Superscript II
(Gibco) reverse transcriptase was added and re-incubated at 42°C for an additional 50 minutes. The RT reaction was stopped by incubation at 70°C for 15 minutes. PCR reactions were set up by adding 2~C1 of the previous RT to a mixture of the following reagents: 50mM .Tris-HC1 (pH 8.0), l.5mM MgCl2, 50mM
KCl, 1~,M each primer, 0.2mM each dNTP and 2 units of Taq polymerase. DNA was amplified in a Perkin-Elmer 9600 PCR machine as follows: One cycle of denaturation at 94°C for two minutes, followed by 30 cycles of denaturation at 94°C for 30 seconds, annealing at 42°C
for 30 seconds, and. elongation at 72°C for one minute.
This was followed by one cycle of elongation at 72°C
for seven minutes. PCR-amplified DNA was analyzed on a 1% agarose gel and purified using a Qiagen purification kit.
Sequencing Protocol Approximately 500ng of plasmid pGEM-T
containing the corresponding DNA segment generated by RT-PCR was added to a 0.2m1 tube containing 3.2pmol of sequencing primer and 8 ~Cl of Terminator ready reaction mix (Perkin-Elmer) and placed into a Perkin-Elmer 9600 PCR machine. The reaction was amplified by cycles at 96°C for 10 seconds, 50°C for five seconds, and 60°C for four minutes. The products were then purified over a G50 Sephadex spin column, lyophilized, and resuspended in 3~C1 of 25mM EDTA
25 (pH8.0) with 50 mg/ml Blue dextran loading dye. The samples were run on an ABI 377 automated sequencer and the sequence analyzed using Sequencher (sequence analysis program from Gene Codes Corporation).
Determination of the nucleotide sequence of the 3' and 5' termini Purified genomic vRNA (2.5~g) (see above) was treated with 10U of tobacco acid pyrophosphatase (Epicenter Technologies, Madison, Wis.) at 37°C for 30 minutes to remove phosphate groups from the 5' terminus. Treated RNA was phenol-chloroform extracted and precipitated with ethanol. Subsequently, the RNA
5' and 3' termini were ligated with 50U of T4 RNA-ligase (Pharmacia Biotech.) at 37°C for one hour, extracted with phenol-chloroform and precipitated with ethanol. Ligated RNA was reverse transcribed and PCR
in a single reaction with a GeneAmp Gold RNA PCR
Reagent Kit ( PE Biosystems) using a forward and reverse pair of primers (see below). Sequencing reactions were carried out as described above, using the primers specific for each RNA segment (Galarza, J.
M., et al., 1996, J of Virol. 70:2360-2368).
Primers for sequencing influenza A/Udorn/72 (H3N2) Primers in the forward direction are marked "F" and are in antigenomic, plus sense. Primers in the reverse direction are marked "R" and are in genomic negative sense.
PB2 (Segment 1) Primers ' Primer F/R Primer sequence by spanned ~ RT Primer 1107 F 5'-TATGGAAAGAATAAA.AGAACTACGGAA-3' 27-53 (SEQ ID N0:23) ~ PCR Primers 1108 R 5'-TCGTTTTTAA.ACTATTCAACAT-3' 2307-2328 (SEQ ID N0:24) 1107 F 5'-TATGGAAAGAATAAA.AGAACTACGGAA-3' 27-53 (SEQ ID N0:23) Sequencing Primers 1101 F 5'-AGCAGGTCAATTATATTCAATATG-3' 1-24 (SEQ ID N0:25) The primer sequence differs from the PB2 gene sequence as follows:
Position PB2 Gene 1101 F

7 ~ A T

18 T . C

1102 R 5'-AACAAGGTCGTTTTTAAACTATTC-3' 2312-2335 (SEQ ID N0:26) 1103 F 5'-AGAACTCTATTCCAACAAATG-3' 1816-1836 (SEQ ID N0:27) 1104 R 5'-AATCGGATATTTCATTGCCAT-3' 178-198 (SEQ ID N0:28; the primer sequence has a C at nucleotide 195, while the PB2 gene sequence has a T) 1105 F 5'-AGAACTCTATTCCAACAAATGAGGGATGTAGTT-3' (SEQ ID N0:29; the primer sequence has a G at nucleotide 1846, while the PB2 gene sequence has a C) 1106 R 5'-AATCGGATATTTCATTGCCATCATCCATTTCAT-3' (SEQ ID N0:30; the primer sequence has a C at nucleotide 195, while the PB2 gene sequence has a T) 1107 F 5'-TATGGAAAGAATAA.A.AGAACTACGGAA-3' 27-53 (SEQ ID N0:23) 1108 R 5'-TCGTTTTTAAA,CTATTCAACAT-3' 2307-2328 (SEQ ID N0:24) 1109 F 5'-AGACGTGGTGTTGGTAATGAA-3' 2214-2234 (SEQ ID N0:31) 1110 F 5'-CGAAGAGTTGACATAAACCCT-3' 454-474 (SEQ ID N0:32) 1111 R 5'-TCATCCCTCATCCCCTCACAT-3' 1943-1963 (SEQ ID N0:33; the primer'sequence has a C at nucleotide 1955, while the PB2 gene sequence has a G) 1112 R 5'-ATTTTCTGTTATCCTCTTGTCA-3' 204-224 (SEQ ID N0:34; this primer has an extra nucleotide (a T) after nucleotide 220 compared to the actual PB2 gene sequence) 1113 F 5' -GTGGAGTCCGCTGTTTTGAG-3' 2083-2102 (SEQ ID N0:35) 1114 F 5' -AGGATGGTGGACATTCTTAGG-3' 904-924 (SEQ ID N0:36) 1115 R 5' -CCGATCAATGCTAACCACTAC-3' 1507-1527 (SEQ ID N0:37) 1116 F 5' -AGCAAAAGCAGGTCAATTATATTCA-3' 1-25 (SEQ ID N0:38) 1117 R 5' -AGTAGAAACAAGGTCGTTTTTAAAC-3' 2317-2341 (SEQ ID N0:39) Primers used to determine terminal sequences 1104 R 5' -AATCGGATATTTCATTGCCAT-3' 17$-198' (SEQ ID N0:28) 1109 F 5' -AGACGTGGTGTTGGTAATGAA-3' 2214-2234 (SEQ ID N0:31) 1112 R 5' -ATTTTCTGTTATCCTCTTGTCA-3' 204-224 (SEQ ID N0:34) 1113 F 5' -GTGGAGTCCGCTGTTTTGAG-3' 2083-2102 (SEQ ID N0:35) PB1 (Segment 2) Primers Primer F/R Primer sequence by spanned ~ RT primer 1215 F 5'-AGCAAAAGCAGGCAAACCAT-3' 1-20 (SEQ ID N0:40; the primer sequence has an A at nucleotide 4, while the PB1 gene sequence has a G) ~ PCR primers 1216 R 5' -AGTAGAA.ACAAGGCATTTTTT-3' 2321-2341 (SEQ ID N0:41) 1215 F 5' -AGCAAAAGCAGGCAAACCAT-3' 1-20 (SEQ ID N0:40) Sequenc ing primers 1201 F 5' -TCGAGCTGAAGAAGCTATGG-3' 1745-1764 (SEQ ID N0:42; the primer sequence has a G at nucleotide 1752, while the PB1 gene sequence has an A, and the primer sequence has an A at nucleotide 1761, while the PB1 gene sequence has a G) 1202 R 5' -GTTCTGTTGACTGTGTCCAT-3' 142-161 (SEQ ID N0:43) 1203 F 5' -TCGAGCTGAAGAAGCTATGGGAGCAGACCCGT-3' (SEQ ID N0:44) The primer sequence differs from the PB1 gene sequence as follows:

Position PB1 Gene 1204 R 5' -GTTCTGTTGACTGTGTCCATGGTGTATCC-3' 133-161 (SEQ ID N0:45) 1205 F 5' -AGCGAAAGCAGGCAA.ACCATTTGAATGGATGTCAA-3' (SEQ ID N0:46) 1206 R 5' -ATTAAAAACAAGGCATTTTTTCATGAAGGAC-3' (SEQ ID N0:47; the primer sequence has an A at nucleotide 2337, while the PB1 gene sequence has a G, and the primer sequence has a T at nucleotide 2340, while the PB1 gene sequence has a G) 1207 F 5' -AATTTCCAGCATGGTGGAGGCCATGGTG-3' 2154-2181 (SEQ ID N0:48) 1208 F 5' -GGGATCTTTGAAAACTCGTG-3' 325-344 (SEQ ID N0:49) 1209 R 5' -CAGCATTGTTTACAGACTC-3' 1936-1954 (SEQ ID N0:50) 1210 F 5' -ACCAAAGATGCAGAAAGAGG-3' 706-725 (SEQ ID N0:51) 1211 R 5' -CTCATATTGATTCCGACTAA-3' 1438-1457 (SEQ ID N0:52) 1212 R 5' -TGTCCACTTCCCTTTTTCTGA-3' 172-192 (SEQ ID N0:53) 1213 F 5' -ATTTTTCCCCAGTAGTTCATAC-3' 2118-2139 (SEQ ID N0:54) 1214 F 5' -ACGCTGTTGCAACTACACACTCCTG-3' 1997-2021 (SEQ ID N0:55) 1215 F 5' -AGCAAAAGCAGGCAAACCAT-3' 1-20 (SEQ ID N0:40) 1216 R 5' -AGTAGAAACAAGGCATTTTTT-3' 2321-2341 (SEQ ID N0:41) Primers used to determine terminal sequences 1204 R 5' -GTTCTGTTGACTGTGTCCATGGTGTATCC-3'133-161 (SEQ ID N0:45) 1212 R 5' -TGTCCACTTCCCTTTTTCTGA-3' 172-192 (SEQ ID N0:53) 1213 F 5' -ATTTTTCCCCAGTAGTTCATAC-3' 2118-2139 (SEQ ID N0:54) 1214 F 5' -ACGCTGTTGCAACTACACACTCCTG-3' 1997-2021 (SEQ ID N0:55) PA (Segment 3) primers Primer F/R Primer sequence by spanned t RT Primer 1315 F 5'-AGCAAAAGCAGGTACTGATTCGAGA-3' 1-25 (SEQ ID N0:56) PCR Primers 1315 F 5'-AGCAAA.AGCAGGTACTGATTCGAGA-3' 1-25 (SEQ ID N0:56) 1316 R 5'-AGTAGAAACAAGGTACTTTTTTGGA-3' 2209-2233 (SEQ ID N0:57) t Sequencing primers 1301 F 5'-GAACCTGGAACCTTTGATCTT-3' 2053-2073 (SEQ ID N0:58) 1302 R 5'-ATTCACCACTGTCCAGGCCAT-3' 280-300 (SEQ ID N0:59) The primer sequence differs from the PA

gene sequence as follows:

Position PA Gene 1302 F

1303 F 5'-GAACCTGGAACCTTTGATCTTGAGGGGCTA-3' (SEQ ID N0:60; the primer sequence has an A at nucleotide 2075, while the PA
gene sequence has a G) 1304 R 5'-ATTCACCACTGTCCAGGCCATTGACGCGTC-3' (SEQ ID N0:61) The primer sequence differs from the PA
gene sequence as follows:
Position PA Gene 1304 F

1305 F 5'-AGCAAAAGCAGGTAGTGATAG-3' 1-21 (SEQ ID N0:62) The primer sequence differs from the PA
gene sequence as follows:
Position PA Gene 1302 F
C G
T A

1306 R 5'-AGTAGAA.ACAAGGTA-3' 2219-2233 (SEQ ID N0:63) 1307 F 5'-AGCGAAAGCAGGTAGTGATTCGAGATGGA-3' 1-29 (SEQ ID N0:64; the primer sequence has a G at nucleotide 4, while the PA

gene sequence has an A) 1308 R 5' -AGTAGAA.ACAAGGTACTTTTTTGGACAG-3'2206-2233 (SEQ ID N0:65) s 1309 R 5' -TCGATTTGTTGGAGTGACTGA-3' 1782-1802 (SEQ ID N0:66) 1310 F 5' -GCCGAACTTCTCCTGCCTTGA-3' 684-704 (SEQ ID N0:67) 1311 F 5' -GTATTCAATAGCCTGTATG-3' 1957-1975 (SEQ ID N0:68) 1312 R 5' -GACTTCTCTCCTTGTCACTC-3' 386-405 (SEQ ID N0:69) 1313 F 5' -ACGAGTCAGCTAAAGTGGGCA-3' 1111-1131 (SEQ ID N0:70) 1314 R 5' -GACACCTCTGCTGTGAAGTAA-3' 1356-1376 ' (SEQ ID N0:71) 1315 F 5' -AGCGAAAGCAGGTAGTGATTCGAGA-3' 1-25 (SEQ ID NO:56) 1316 R 5' -AGTAGAAACAAGGTACTTTTTTGGA-3' 2209-2233 (SEQ ID N0:57) 1317 F 5' -GGAGCTGAGAAACCGAAGTTT-3' 319-339 (SEQ ID N0:72) 1318 R 5' -GACTTGGCCAATAAAGTCCTA-3' 1935-1955 (SEQ ID N0:73) 1319 R 5' -GCCTGCGCATAGTTCCTGTGA-3' 644-664 (SEQ ID N0:74) 1320 R 5' -TCTACCACTATTGACTCGCC-3' 196-215 (SEQ ID N0:75) Primers used to determine terminal sequences 1301 F 5' -GAACCTGGAACCTTTGATCTT-3' 2053-2073 (SEQ ID N0:58) 1303 F 5' -GAACCTGGAACCTTTGATCTTGAGGGGGCTA-3' (SEQ ID N0:60) 1311 F 5' -GTATTCAATAGCCTGTATG-3' 1957-1975 (SEQ ID N0:68) 1312 R 5' -GACTTCTCTCCTTGTCACTC-3' 386-405 (SEQ ID N0:69) 1320 R 5' -TCTACCACTATTGACTCGCC-3' 196-215 (SEQ ID N0:75) HA (Segment 4) Primers Primer F/R Primer sequence by spanned RTprimer 1401 F 5' -AGCAAAAGCAGGGGATAATTCTA-3' 1-23 (SEQ ID N0:76) PCR
primers 1401 F 5' -AGCAAAAGCAGGGGATAATTCTA-3' 1-23 (SEQ ID N0:76) 1402 R 5' -AGTAGAA.ACAAGGGTGTTTTTAA-3' 1743-1765 (SEQ ID N0:77) Sequex~,cix~,g Primers 1401 F 5' -AGCAAAAGCAGGGGATATTCTA-3' 1-23 (SEQ ID N0:76) 1402 R 5' -AGTAGAAACAAGGGTGTTTTTAA-3' 1743-1765 (SEQ ID N0:77) 1403 R 5' -ACAGTTTGTTCATTTCCGAG-3' 1400-1419 (SEQ ID N0:78) 1404 R 5' -ACTTCAGGGTGTTTTGCTTA-3'. 1004-1023 (SEQ ID N0:79) 1405 R 5' -GAACCCCCCAAATGTATAGT-3' 605-624 (SEQ ID N0:80) 1406 R 5' -TGAGGAACTCTGAACCAGCT-3' 199-218 (SEQ ID N0:81) 1407 F 5' -GTCACTAGTTGCCTCGTCAG-3' 404-423 (SEQ ID N0:82) 1408 F 5' -CCGGGAGACATACTGGTAAT-3' 792-811 (SEQ ID N0:83) 1409 F 5' -CAAATCAATGGGAAACTGAA-3' 1203-1222 (SEQ ID N0:84) 1410 F 5' -TGAACTGAAGTCAGGATACA-3' 1592-1611 (SEQ ID N0:85) Primersused to determine terminal sequences 1405 R 5' -GAACCCCCCAAATGTATAGT-3' 605-624 (SEQ ID N0:80) 1406 R 5' -TGAGGAACTCTGAACCAGCT-3' 199-218 (SEQ ID N0:81) 1409 F 5' -CAAATCAATGGGAAACTGAA-3' 1203-1222 (SEQ ID N0:84) 1410 F 5' -TGAACTGAAGTCAGGATACA-3' 1592-1611 (SEQ ID N0:85) NP (Segment 5) Primers Primer F/R Primer sequence by spanned ~ RT Primer 1509 F 5'-AGCAAAAGCAGGGTTAATAATCAC-3' 1-24 (SEQ ID N0:86) ~ PCR Primers 1509 F 5'-AGCA.AAAGCAGGGTTAATAATCAC-3' 1-24 (SEQ ID N0:86) 1510 R .5'-AGTAGAAACAAGGGTATTTTTCCT-3' 1542-1565 (SEQ ID N0:87) ~ Sequencing primers 1501 R 5'-TTGCACCTTCCATCATCCTT-3' 1380-1399 (SEQ ID N0:88) 1502 R 5' -GTCTATTCCCACTAAAGAGT-3' 932-951 (SEQ ID N0:89) 1503 R 5' -TGCATCAGAGAGCACATCCT-3' 529-548 (SEQ ID N0:90) 1504 F 5' -GAACTCGTCCTTTATGACAA-3' 364-383 (SEQ ID N0:91) 1505 F 5' -AGAGCAATGGATCAAGT-3' 751-770 (SEQ ID N0:92; there is a deletion of the three bases ATG at nucleotides 763) 1506 F 5' -TACTATGGAATCAAGTACTC-3' 1161-1180 (SEQ ID N0:93) 1507 R 5' -ATCAATCATCTTCCCGAC-3' 130-147 (SEQ ID N0:94) 1508 F 5' -AGTGTCCTTCCGTGGGCG-3' 1410-1427 (SEQ ID N0:95) 1509 F 5' -AGCAAAAGCAGGGTTAATAATCAC-3'1-24 (SEQ ID N0:86) 1510 R 5' -AGTAGAAACAAGGGTATTTTTCCT-3'1542-1565 (SEQ ID N0:87) t Primers used to determine terminal sequences 1503 R 5' -TGCATCAGAGAGCACATCCT-3' 529-548 (SEQ ID N0:90) 1506 F 5'-TACTATGGAATCAAGTACTC-3' 1161-1180 (SEQ ID N0:93) NA (Segment 6) primers Primer Primer sequence by spanned F/R

t RT Primer 1601 F 5' -AGCAAAAGCAGGAGTGAAGATGA-3' 1-23 (SEQ ID N0:96) t PCR mers pri 1601 F 5' -AGCAAAAGCAGGAGTGAAGATGA-3' 1-23 (SEQ ID N0:96) 1602 R 5' -AGTAGAAACAAGGAGTTTTTTCTA-3' 1443-1466 (SEQ ID N0:97) Sequencing primers 1601 F 5' -AGCAAAAGCAGGAGTGAAGATGA-3' 1-23 (SEQ ID N0:96) 1602 R 5' -AGTAGAAACAAGGAGTTTTTTCTA-3' 1443-1466 (SEQ ID N0:97) 1603 R 5' -TCGTTGTTTCTGGGTGTGTC-3' 989-1008 (SEQ ID N0:98; the primer sequence has a T at nucleotide 992, while the NA

gene sequence has an C) 1604 R 5' -TTATCATACCCAGTGACACA-3' 596-615 (SEQ ID N0:99) 1605 R 5' -ATTATTATTGGTTCACACGG-3' 173-192 (SEQ ID N0:100) 1606 F 5' -TTATGTGTCATGCGATCCTG-3' 379-398 (SEQ ID N0:101) 1607 F 5' -ATTGTTCATATTAGCCCATT-3' 803-822 (SEQ ID N0:102) 1608 F 5' -ATAGGTCAGGTTATTCTGGT-3' 1224-1243 (SEQ ID N0:.103) Primers used to determine terminal sequences 1608 F 5' -ATAGGTCAGGTTATTCTGGT-3' 1224-1243 (SEQ ID N0:103) 1605 R 5' -ATTATTATTGGTTCACACGG-3' 173-192 (SEQ ID N0:100) M1 (Segment 7) Primers Primer F/R Primer sequence by spanned ~ RT Primer *1706 F 5'-AGCAAAAGCAGGTAG-3' 1-15 (SEQ ID N0:104) ~ PCR primers 1707 R 5'-AGTAGAA.ACAAGGTA-3' 1013-1027 (SEQ ID N0:105) 1706 F 5' -AGCAAAAGCAGGTAG-3' 1-15 (SEQ ID N0:104) *1701 R 5' -TTTACTCCAGCTCTATGCTGACAA-3' 985-1008 (SEQ ID N0:106) * The se primers and M2 genes.
will amplify both the t Sequenc ing primers 1701 R 5' -TTTACTCCAGCTCTATGCTGACAA-3' 985-1008 (SEQ ID N0:106) 1702 R 5' -GATCCAGCCATTTGCTCCAT-3' 590-609 (SEQ ID N0:107) 1703 R 5' -GAGGTGACAGGATTGGTCTT-3' 169-188 (SEQ ID N0:108) 1704 F 5' -CATGGACAGAGCAGTTAAAC-3' 301-320 (SEQ ID N0:109) 1705F 5'-GCGAGTATCATTGGGATCTT-3' 801-820 (SEQ ID N0:110) Common to M1 and M2 sequence.
Primers used to determine terminal sequences 1705 F 5'-GCGAGTATCATTGGGATCTT-3' 801-820 ' (SEQ ID N0:110) 1702 R 5'-GATCCAGCCATTTGCTCCAT-3' 590-609 (SEQ ID N0:107) 1703 R 5'-GAGGTGACAGGATTGGTCTT-3 169-188 (SEQ ID N0:108) Primers 1701, 1705 and 1706 are sufficient to sequence M2.
NS1 (Segment 8) Primers Primer F/R Primer sequence by spanned RT Primer *1801 F 5'-AGCAAAAGCAGGGTGACAAAGACA-3' 1-24 (SEQ ID N0:111) PCR Primers 1801 F 5' -AGCAAAAGCAGGGTGACAAAGACA-3' 1-24 (SEQ ID N0:111) 1802 R 5' -AGTAGAAACAAGGGTGTTTTTTAT-3' 867-890 (SEQ ID N0:112) *1803 R 5' -TTTTTTATCATTAAATAAGCTGAA-3' 857.-874 (SEQ ID~ N0:113) * These primers and NS2 will amplify both the genes.

~ Sequenc ing Primers 1801 F 5' -AGCAAAAGCAGGGTGACAAAGACA-3' 1-24 (SEQ ID N0:111) 1802 R 5' -AGTAGAAACAAGGGTGTTTTTTAT-3' 867-890 (SEQ ID N0:112) 1803 R 5'-TTTTTTATCATTAAATAAGCTGAA-3' 851-874 (SEQ ID N0:1I3) 1804 R 5'-AATTGCATTTTTGACATCCT-3' 541-560 (SEQ ID N0:114) 1805 R 5'-ATCTTCTCTACTATCTGCTT-3' 210-229 (SEQ ID N0:115) 1806 F 5'-CAAGCAATCATGGATAAGAA-3' 387-406 (SEQ ID N0:116) 1807 F 5'-GGCGAGAACAGCTAGGTCAA-3' 692-711 (SEQ ID N0:117) Common to NS1 and NS2 sequence t Pri mers used to determine terminal sequences 1807 F 5'-GGCGAGAACAGCTAGGTCAA-3' 692-711 (SEQ ID N0:117) 1804 R 5'-AATTGCATTTTTGACATCCT-3' 541-560 (SEQ ID N0:114) 1806 F 5'-CAAGCAATCATGGATAAGAA-3' 387-406 (SEQ ID N0:116) 1805 R 5'-ATCTTCTCTACTATCTGCTT-3' 210-229 (SEQ ID N0:115) Primers 1801, 1803 and 1807 are sufficient to sequence NS2.

Example 2 PCR Assay to Detect a Segment of the Influenza A/Udorn/72 (H3N2) Strain A PCR assay is used to detect the presence of a segment of the Influenza A/Udorn/72 (H3N2) strain. PCR primers are designed and selected based on homologies to the viral sequences described herein.
The assay is conducted by subjecting the sample to reverse transcription of RNA, followed by PCR
amplification of selected cDNA regions corresponding to the specific nucleotide sequence described herein.
Amplified PCR products are identified on gels and their specificity confirmed by hybridization with specific nucleotide probes.
Example 3 ELISA to Detect an Antigen Produced by a Segment of the Influenza A/Udorn/72 (H3N2) Strain An ELISA test is used to detect the presence of an antigen produced by a segment of the Influenza A/Udorn/72 (H3N2) strain. Peptides are designed and selected based on homologies to the viral sequences described herein. These peptides are then coupled to KLH and used to immunize rabbits for the production of monospecific polyclonal antibody. A selection of these polyclonal antibodies, or a combination of polyclonal and monoclonal antibodies is then used in a "capture ELISA" to detect the presence of a protein produced by that viral segment.

SEQUENCE LISTING
<110> American Cyanamid Company <120> Nucleotide Sequence of Influenza A/Udorn/72 (H3N2) Genome <130> AM100289PCT
<140>
<141>
<150> 60/213,650 <151> 2000-06-23 <160> 117 <170> PatentIn Ver. 2.1 <210> 1 <211> 2341 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (28) .. (2304) <400> 1 agcaaaagca ggtcaattat attcaat atg gaa aga ata aaa gaa cta cgg aat 54 Met Glu Arg Ile Lys Glu Leu Arg Asn ctg atg tcg cag tct cgc act cgc gag ata cta aca aaa acc aca gtg 102 Leu Met Ser Gln Ser Arg Thr Arg Glu Ile Leu Thr Lys Thr Thr Val gac cat atg gcc ata att aag aag tac aca tca ggg aga cag gaa aag 150 Asp His Met Ala Ile Ile Lys Lys Tyr Thr Ser Gly Arg Gln Glu Lys aac ccg tca ctt agg atg aaa tgg atg atg gca atg aaa tat cca att 198 Asn Pro Ser Leu Arg Met Lys Trp Met Met Ala Met Lys Tyr Pro Ile aca get gac aag agg ata aca gaa atg gtt cct gag aga aat gag caa 246 Thr Ala Asp Lys Arg Ile Thr Glu Met Val Pro Glu Arg Asn Glu Gln gga caa acc cta tgg agt aaa atg agt gat gcc ggg tca gat cga gtg 294 Gly Gln Thr Leu Trp Ser Lys Met Ser Asp Ala Gly Ser Asp Arg Val atg gta tca cct ttg gcg gtg aca tgg tgg aat aga aat gga cca gtg 342 Met Val Ser Pro Leu Ala Val Thr Trp Trp Asn Arg Asn Gly Pro Val aca agt acg gtt cat tat cca aaa gtc tac aag act tat ttt gat aaa 390 Thr Ser Thr Va1 His Tyr Pro Lys Va1 Tyr Lys Thr Tyr Phe Asp Lys gtc gaa agg tta aaa cat gga acc ttt ggc cct gtc cat ttt aga aac 438 Val Glu Arg Leu Lys His Gly Thr Phe Gly Pro Val His Phe Arg Asn 125 . 130 135 caa gtc aaa ata cgc cga aga gtt gac ata aac cct ggt cat gca gac 486 Gln Val Lys Ile Arg Arg Arg Val Asp Ile Asn Pro Gly His Ala Asp ctc agt gcc aag gag gca caa gat gta atc atg gaa gtt gtt ttc ccc 534 Leu Ser Ala Lys Glu Ala Gln Asp Val Ile Met Glu Val Val Phe Pro aat gaa gtg ggg gcc agg ata cta acg tcg gaa tca caa tta aca ata 582 Asn Glu Val Gly Ala Arg Ile Leu Thr Ser Glu Ser Gln Leu Thr Ile acc aaa gag aaa aaa gaa gaa ctc caa gat tgc aaa att tct cct ttg 630 Thr Lys Glu Lys Lys Glu Glu Leu Gln Asp Cys Lys Ile Ser Pro Leu atg gtt gca tac atg tta gag aga gaa ctt gtc cga aaa acg aga ttt 678 Met Val Ala Tyr Met Leu Glu Arg Glu Leu Val Arg Lys Thr Arg Phe ctc cca gtt get ggt gga aca agc agt gtg tac att gaa gtg tta cac 726 Leu Pro Val Ala Gly Gly Thr Ser Ser Val Tyr Ile Glu Val Leu His ttg act caa gga acg tgt tgg gaa cag atg tac act cca ggt gga gaa 774 Leu Thr Gln Gly Thr Cys Trp Glu Gln Met Tyr Thr Pro Gly Gly Glu gtg agg aat gac gat gtt gac caa agc cta att att gca gcc agg aac 822 Val Arg Asn Asp Asp Val Asp Gln Ser Leu Ile Ile Ala Ala Arg Asn ata gtg aga aga gca gca gta tca gca gat cca cta gca tct tta ttg 870 Ile Val Arg Arg Ala Ala Val Ser Ala Asp Pro Leu Ala Ser Leu Leu gag atg tgc cac agc aca ctg att ggc ggg aca agg atg gtg gac att 918 Glu Met Cys His Ser Thr Leu Ile Gly Gly Thr Arg Met Val Asp Ile ctt agg cag aac ccg acg gaa gaa caa get gtg gat ata tgc aag get 966 Leu Arg Gln Asn Pro Thr Glu Glu Gln Ala Val Asp Ile Cys Lys Ala gca atg gga ctg agg atc agc tca tcc ttc agt ttt ggt ggg ttc aca 1014 Ala Met Gly Leu Arg Ile Ser Ser Ser Phe Ser Phe Gly Gly Phe Thr ttt aag aga aca agc ggg tca tca atc aaa aga gag gaa gaa gtg ctt 2062 Phe Lys Arg Thr Ser Gly Ser Ser Ile Lys Arg Glu Glu Glu Val Leu acg ggc aat ctc caa aca ttg aaa ata agg gtg cat gag ggg tac gag 1110 Thr Gly Asn Leu Gln Thr Leu Lys Ile Arg Val His Glu Gly Tyr Glu gag ttc aca atg gtg ggg aaa agg gca aca get ata ctc aga aaa gca 1158 Glu Phe Thr Met Val Gly Lys Arg Ala Thr Ala Ile Leu Arg Lys Ala acc agg aga ttg gtt cag ctt ata gtg agt gga agg gac gag cag tca 1206 Thr Arg Arg Leu Val Gln Leu Ile Val Ser Gly Arg Asp Glu Gln Ser ata gcc gaa gcg ata att gta gcc atg gtg ttt tca caa gag gat tgc 1254 Ile Ala Glu Ala Ile Ile Val Ala Met Val Phe Ser Gln Glu Asp Cys atg ata aaa gca gtt aga ggt gac ctg aat ttc gtt aac agg gca aat 1302 Met Ile Lys Ala Val Arg Gly Asp Leu Asn Phe Val Asn Arg Ala Asn cag cgg ttg aat ccc atg cat caa ctt tta agg cat ttt cag aaa gat 1350 Gln Arg Leu Asn Pro Met His Gln Leu Leu Arg His Phe Gln Lys Asp gcg aaa gtg ctt ttt cag aat tgg gga att gaa cat atc gac aat gtg 1398 Ala Lys Val Leu Phe Gln Asn Trp Gly Ile Glu His Ile Asp Asn Val atg gga atg gtt gga gta tta cca gac atg act cca agc aca gag atg ' 1446 Met Gly Met Val Gly Val Leu Pro Asp Met Thr Pro Ser Thr Glu Met tca atg aga gga ata aga gtc agc aaa atg ggc gtg gat gaa tac tcc 1494 Ser Met Arg Gly Ile Arg Val Ser Lys Met Gly Val Asp Glu Tyr Ser agc aca gag agg gta gtg gtt agc att gat cgg ttt ttg aga gtt cga 1542 Ser Thr Glu Arg Val Val Val Ser Ile Asp Arg Phe Leu Arg Val Arg gac caa cgt ggg aat gta tta cta tct cct gag gag gtc agt gaa aca 1590 Asp Gln Arg Gly Asn Val Leu Leu Ser Pro Glu Glu Val Ser Glu Thr cag ggg aca gag aga ctg aca ata act tac tca tcg tca atg atg tgg 1638 Gln Gly Thr Glu Arg Leu Thr Ile Thr Tyr Ser Ser Ser Met Met Trp gag att aat ggc cct gag tca gtg ttg gtc aat acc tat caa tgg atc 1686 Glu Ile Asn Gly Pro Glu Ser Val Leu Val Asn Thr Tyr Gln Trp Ile atc aga aac tgg gaa act gtt aaa att caa tgg tct cag aat cct aca 1734 Ile Arg Asn Trp Glu Thr Val Lys Ile Gln Trp Ser Gln Asn Pro Thr atg ttg tac aac aaa atg gaa ttt gag cca ttt cag tct tta gtt cct 1782 Met Leu Tyr Asn Lys Met Glu Phe Glu Pro Phe Gln Ser Leu Val Pro aag gcc att aga ggc caa tac agt gga ttt gtc aga act cta ttc caa 1830 Lys Ala Ile Arg Gly Gln Tyr Ser Gly Phe Val Arg Thr Leu Phe Gln caa atg agg gat gta ctt ggg aca ttt gat acc acc cag ata ata aag 1878 Gln Met Arg Asp Val Leu Gly Thr Phe Asp Thr Thr Gln Ile Ile Lys ctt ctc ccc ttt gca gcc gcc cca cca aag caa agt aga atg cag ttc 1926 Leu Leu Pro Phe Ala Ala Ala Pro Pro Lys Gln Ser Arg Met Gln Phe tct tca ttg act gtg aat gtg agg gga tca ggg atg aga ata ctt gta 1974 Ser Ser Leu Thr Val Asn Val Arg Gly Ser Gly Met Arg Ile Leu Val agg ggc aat tct cct gta ttc aac tac aac aag acc act aaa aga cta 2022 Arg Gly Asn Ser Pro Val Phe Asn Tyr Asn Lys Thr Thr Lys Arg Leu aca att ctc gga aaa gat get ggc act tta att gaa gac cca gat gaa 2070 Thr Ile Leu Gly Lys Asp Ala Gly Thr Leu'Ile Glu Asp Pro Asp Glu agc aca tcc gga gtg gag tcc get gtt ttg aga gga ttt ctc att cta 2118 Ser Thr Ser Gly Val Glu Ser Ala Val Leu Arg Gly Phe Leu Ile Leu ggt aag gaa gat aga aga tac gga cca gca tta agc atc aat gaa ctg 2166 Gly Lys Glu Asp Arg Arg Tyr Gly Pro Ala Leu Ser Ile Asn Glu Leu agt aac ctt gca aaa gga gaa aag get aat gtg cta att ggg caa gga 2214 Ser Asn Leu Ala Lys Gly Glu Lys Ala Asn Va1 Leu Ile Gly Gln Gly gac gtg gtg ttg gta atg aaa cga aaa cgg gac tct agc ata ctt act 2262 Asp Val Val Leu Val Met Lys Arg Lys Arg Asp Ser Ser Ile Leu Thr gac agc cag aca gcg acc aaa aga att cgg atg gcc atc aat 2304 Asp Ser Gln Thr Ala Thr Lys Arg Tle Arg Met Ala Ile Asn taatgttgaa tagtttaaaa acgaccttgt ttctact 2341 <210> 2 <211> 759 <212> PRT
<213> Influenza A virus <400> 2 Met Glu Arg Ile Lys Glu Leu Arg Asn Leu Met Ser Gln Ser Arg Thr Arg Glu Ile Leu Thr Lys Thr Thr Val Asp His Met Ala Ile Ile Lys Lys Tyr Thr Ser Gly Arg Gln Glu Lys Asn Pro Ser Leu Arg Met Lys Trp Met Met Ala Met Lys Tyr Pro Ile Thr Ala Asp Lys Arg Ile Thr Glu Met Val Pro Glu Arg Asn Glu Gln Gly Gln Thr Leu Trp Ser Lys Met Ser Asp Ala Gly Ser Asp Arg Val Met Val Ser Pro Leu Ala Val Thr Trp Trp Asn Arg Asn Gly Pro Val Thr Ser Thr Val His Tyr Pro Lys Val Tyr Lys Thr Tyr Phe Asp Lys Val Glu Arg Leu Lys His Gly Thr Phe Gly Pro Val His Phe Arg Asn Gln Val Lys Ile Arg Arg Arg Val Asp Ile Asn Pro Gly His Ala Asp Leu Ser Ala Lys Glu Ala Gln Asp Val Ile Met Glu Val Val Phe Pro Asn Glu Val Gly Ala Arg Ile Leu Thr Ser Glu Ser Gln Leu Thr Ile Thr Lys Glu Lys Lys Glu Glu Leu Gln Asp Cys Lys Ile Ser Pro Leu Met Val Ala Tyr Met Leu Glu Arg Glu Leu Val Arg Lys Thr Arg Phe Leu Pro Val Ala Gly Gly Thr Ser Ser Val Tyr Ile Glu Val Leu His Leu Thr Gln Gly Thr Cys Trp Glu Gln Met Tyr Thr Pro Gly Gly Glu Val Arg Asn Asp Asp Val Asp Gln Ser Leu Ile Ile Ala Ala Arg Asn Ile Val Arg Arg Ala Ala Val Ser Ala Asp Pro Leu Ala Ser Leu Leu Glu Met Cys His Ser Thr Leu Ile Gly Gly Thr Arg Met Val Asp Ile Leu Arg Gln Asn Pro Thr Glu Glu Gln Ala Val Asp Ile Cys Lys Ala Ala Met Gly Leu Arg Ile Ser Ser Ser Phe Ser Phe Gly Gly Phe Thr Phe Lys Arg Thr Ser Gly Ser Ser Ile Lys Arg Glu Glu Glu Val Leu Thr Gly Asn Leu Gln Thr Leu Lys Ile Arg Val His Glu Gly Tyr Glu Glu Phe Thr Met Val Gly Lys Arg Ala Thr Ala Ile Leu Arg Lys Ala Thr Arg Arg Leu Val Gln Leu Ile Val Ser Gly Arg Asp Glu Gln Ser Ile Ala Glu Ala Ile Ile Val Ala Met Val Phe Ser Gln Glu Asp Cys Met Ile Lys Ala Val Arg Gly Asp Leu Asn Phe Val Asn Arg Ala Asn Gln Arg Leu Asn Pro Met His Gln Leu Leu Arg His Phe Gln Lys Asp Ala Lys Val Leu Phe Gln Asn Trp Gly Ile Glu His Ile Asp Asn Val Met Gly Met Val Gly Val Leu 450 455 ' 460 Pro Asp Met Thr Pro Ser Thr G1u Met Ser Met Arg Gly Ile Arg Val 465 470 ~ 475 480 Ser Lys Met Gly Val Asp Glu Tyr Ser Ser Thr Glu Arg Val Val Val Ser Ile Asp Arg Phe Leu Arg Val Arg Asp Gln Arg Gly Asn Val Leu Leu Ser Pro Glu Glu Val Ser Glu Thr Gln Gly Thr Glu Arg Leu Thr Ile Thr Tyr Ser Ser Ser Met Met Trp Glu Ile Asn Gly Pro Glu Ser Val Leu Val Asn Thr Tyr Gln Trp Ile Ile Arg Asn Trp Glu Thr Val Lys Ile Gln Trp Ser Gln Asn Pro Thr Met Leu Tyr Asn Lys Met Glu Phe Glu Pro Phe Gln Ser Leu Val Pro Lys Ala Ile Arg Gly Gln Tyr Ser Gly Phe Val Arg Thr Leu Phe Gln Gln Met Arg Asp Val Leu Gly Thr Phe Asp Thr Thr Gln Ile Ile Lys Leu Leu Pro Phe Ala Ala Ala Pro Pro Lys Gln Ser Arg Met Gln Phe Ser Ser Leu Thr Val Asn Val 625 630 635 ' 640 Arg Gly Ser Gly Met Arg Ile Leu Val Arg Gly Asn Ser Pro Val Phe Asn Tyr Asn Lys Thr Thr Lys Arg Leu Thr Ile Leu Gly Lys Asp Ala Gly Thr Leu Ile Glu Asp Pro Asp Glu Ser Thr Ser Gly Val Glu Ser Ala Val Leu Arg Gly Phe Leu Ile Leu Gly Lys Glu Asp Arg Arg Tyr Gly Pro Ala Leu Ser Ile Asn Glu Leu Ser Asn Leu Ala Lys Gly Glu Lys Ala Asn Val Leu Ile Gly Gln Gly Asp Val Val Leu Val Met Lys Arg Lys Arg Asp Ser Ser Ile Leu Thr Asp Ser Gln Thr Ala Thr Lys Arg Ile Arg Met Ala Ile Asn <210> 3 <211> 2341 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (25)..(2295) <400> 3 agcgaaagca ggcaaaccat ttga atg gat gtc aac ccg act tta ctt ttc 51 Met Asp Val Asn Pro Thr Leu Leu Phe ttg aaa gtt cca gcg caa aat gcc ata agc acc aca ttc cct tat act 99 Leu Lys Val Pro Ala Gln Asn Ala Ile Ser Thr Thr Phe Pro Tyr Thr gga gat cct cca tac agc cat gga aca gga aca gga tac acc atg gac 147 Gly Asp Pro Pro Tyr Ser His Gly Thr Gly Thr Gly Tyr Thr Met Asp aca gtc aac aga aca cat caa tat tca gaa aaa ggg aag tgg aca aca 195 Thr Val Asn Arg Thr His Gln Tyr Ser Glu Lys Gly Lys Trp Thr Thr aac aca gaa act ggg gcg CCC Caa Ctt aac cca att gat gga cca cta 243 Asn Thr Glu Thr Gly Ala Pro Gln Leu Asn Pro Ile Asp Gly Pro Leu cct gag gat aat gag cca agt gga tat gca caa aca gac tgt gtc ctg 291 Pro Glu Asp Asn Glu Pro Ser Gly Tyr Ala Gln Thr Asp Cys Val Leu gaa gca atg get ttc ctt gaa gaa tcc cac cca ggg atc ttt gaa aac 339 Glu Ala Met Ala Phe Leu Glu Glu Ser His Pro Gly Ile Phe Glu Asn tcg tgc ctt gaa acg atg gaa gtc gtt caa caa aca agg gtg gac aga 387 Ser Cys Leu Glu Thr Met Glu Val Val Gln Gln Thr Arg Val Asp Arg ctg acc caa ggt cgt cag acc tat gat tgg aca tta aac aga aat caa 435 Leu Thr Gln Gly Arg Gln Thr Tyr Asp Trp Thr Leu Asn Arg Asn Gln f CCg gCC gca act gca tta gcc aac act ata gaa gtc ttc aga tcg aat 483 Pro Ala Ala Thr Ala Leu Ala Asn Thr Ile Glu Val Phe Arg Ser Asn ggt cta aca get aat gag tcg gga agg cta ata gat ttc ctc aag gat 531 Gly Leu Thr Ala Asn Glu Ser Gly Arg Leu Ile Asp Phe Leu Lys Asp gtg atg gaa tca atg gat aaa gag gaa atg gag ata aca aca cac ttc 579 Val Met Glu Ser Met Asp Lys Glu Glu Met Glu Ile Thr Thr His Phe caa aga aaa aga aga gta aga gac aac atg acc aag aaa atg gtc aca 627 Gln Arg Lys Arg Arg Val Arg Asp Asn Met Thr Lys Lys Met Val Thr caa aga aca ata gga aag aag aag cag aga gtg aac aag aga agc tat 675 Gln Arg Thr Ile Gly Lys Lys Lys Gln Arg Val Asn Lys Arg Ser Tyr cta ata aga gca tta aca ttg aac aca atg acc aaa gat gca gaa aga 723 Leu Ile Arg Ala Leu Thr Leu Asn Thr Met Thr Lys Asp Ala Glu Arg ggt aaa tta aag aga aga get att gca aca ccc ggg atg caa atc aga 771 Gly Lys Leu Lys Arg Arg Ala Ile Ala Thr Pro Gly Met Gln Ile Arg ggg ttc gtg tac ttt gtt gaa act cta get agg agc att tgt gag aag 819 Gly Phe Val Tyr Phe Val Glu Thr Leu Ala Arg Ser Ile Cys Glu Lys ctt gaa cag tct gga ctt cca gtt gga ggt aat gaa aag aag gcc aaa 867 Leu Glu Gln Ser Gly Leu Pro Val Gly Gly Asn Glu Lys Lys Ala Lys ctg gca aat gtt gtg aga aag atg atg act aat tca caa gac aca gag 915 Leu Ala Asn Val Val Arg Lys Met Met Thr Asn Ser Gln Asp Thr Glu ctt tct ttc aca att act gga gac aat act aag tgg aat gaa aat caa 963 Leu Ser Phe Thr Ile Thr Gly Asp Asn Thr Lys Trp Asn Glu Asn Gln aat cct cga atg ttc ctg gcg atg att aca tat atc aca aaa aat.caa 1011 Asn Pro Arg Met Phe Leu Ala Met Ile Thr Tyr Ile Thr Lys Asn Gln cct gaa tgg ttc aga aac att ctg agc ate gca ccc ata atg ttc tca 1059 Pro Glu Trp Phe Arg Asn Ile Leu Ser Ile Ala Pro I1e Met Phe Ser aac aaa atg gcg aga cta ggg aaa gga tac atg ttc gaa agt aag aga 1107 Asn Lys Met Ala Arg Leu Gly Lys Gly Tyr Met Phe Glu Ser Lys Arg atg aag ctc cga aca caa ata cca gca gaa atg cta gca agc att gac 1155 Met Lys Leu Arg Thr Gln Ile Pro Ala Glu Met Leu Ala Ser Ile Asp cta aag tat ttc aat gaa tca aca aga aag aaa att gag aaa ata aag 1203 Leu Lys Tyr Phe Asn Glu Ser Thr Arg Lys Lys Ile Glu Lys Ile Lys cct ctt cta ata gat ggc aca gcg tca ttg agt cct gga atg atg atg 1251 Pro Leu Leu Ile Asp Gly Thr Ala Ser Leu Ser Pro Gly Met Met Met ggc atg ttc aac atg cta agt acg gtt tta gga gtc tca atc ctg aat 1299 Gly Met Phe Asn Met Leu Ser Thr Val Leu Gly Val Ser Ile Leu Asn ctt ggg caa aag aaa tac acc aaa aca aca tac tgg tgg gat gga ctc 1347 Leu Gly Gln Lys Lys Tyr Thr Lys Thr Thr Tyr Trp Trp Asp Gly Leu caa tcc tct gat gat ttt get ctc ata gtg aat gca cca aat cat gag 1395 Gln Ser Ser Asp Asp Phe Ala Leu Ile Val Asn Ala Pro Asn His Glu gga ata caa gca gga gtg gat aga ttc tac agg acc tgc aag tta gtc 1443 Gly Ile Gln Ala Gly Val Asp Arg Phe Tyr Arg Thr Cys Lys Leu Val gga atc aat atg agc aag aag aag tcc tat ata aat agg aca gga aca 1491 Gly Ile Asn Met Ser Lys Lys Lys Ser Tyr Ile Asn Arg Thr Gly Thr ttt gaa ttc aca agc ttt ttt tat cgc tat gga ttt gta gcc aat ttt 1539 Phe Glu Phe Thr Ser Phe Phe Tyr Arg Tyr Gly Phe Val Ala Asn Phe agc atg gag ctg ccc agt ttt gga gtg tct ggg att aat gag tca get 1587 Ser Met Glu Leu Pro Ser Phe Gly Val Ser Gly Ile Asn Glu Ser Ala gat atg agc att gga gta aca gtg ata aag aac aac atg ata aac aat 1635 Asp Met Ser Ile Gly Val Thr Val Ile Lys Asn Asn Met Ile Asn Asn gac ctt gga cca gca aca gcc cag atg get ctt caa ctg ttc ate aag 1683 Asp Leu Gly Pro Ala Thr Ala Gln Met Ala Leu Gln Leu Phe Ile Lys gac tac aga tat aca tat cgg tgc cac aga gga gac aca caa att cag 1731 Asp Tyr Arg Tyr Thr Tyr Arg Cys His Arg Gly Asp Th'r Gln Ile Gln acg agg aga tca ttc gag cta aag aag ctg tgg gag caa acc cgc tca 1779 Thr Arg Arg Ser Phe Glu Leu Lys Lys Leu Trp Glu Gln Thr Arg Ser aag gca gga cta ttg gtt tca gat gga gga cca aac tta tac aat atc 1827 Lys Ala Gly Leu Leu Val Ser Asp Gly Gly Pro Asn Leu Tyr Asn Ile cgg aat ctt cac atc cct gaa gtc tgc tta aag tgg gag cta atg gat 1875 Arg Asn Leu His Ile Pro Glu Val Cys Leu Lys Trp Glu Leu Met Asp gag gac tat cag gga aga ctt tgt aat ccc ctg aat cca ttt gtc agc 1923 Glu Asp Tyr Gln Gly Arg Leu Cys Asn Pro Leu Asn Pro Phe Val Ser cat aag gag att gag tct gta aac aat get gtg gta atg cca get cat 1971 His Lys Glv. Ile Glu Ser Val Asn Asn Ala Val Val Met Pro Ala His ggt cca gcc aag agc atg gaa tat gac get gtt gca act aca cac tcc 2019 Gly Pro Ala Lys Ser Met Glu Tyr Asp Ala Val Ala Thr Thr His Ser 650 655 660 ' 665 tgg att ccc aag agg aac cgc tct att ctc aac aca agc caa agg gga 2067 Trp Ile Pro Lys Arg Asn Arg Ser Ile Leu Asn Thr Ser Gln Arg Gly att ctt gag gat gaa cag atg tat cag aag tgc tgc aac ctg ttc gag 2115 Ile Leu Glu Asp Glu Gln Met Tyr Gln Lys Cys Cys Asn Leu Phe Glu aaa ttt ttc ccc agt agt tca tac agg aga ccg gtt gga att tcc agc 2163 Lys Phe Phe Pro Ser Ser Ser Tyr Arg Arg Pro Val Gly Ile Ser Ser atg gtg gag gcc atg gtg tct agg gcc cgg att gat gcc aga att gac 2211 Met Val Glu Ala Met Val Ser Arg Ala Arg Ile Asp Ala Arg Ile Asp ttc gaa tct gga cgg att aag aaa gaa gag ttc gcc gag atc atg aag 2259 Phe Glu Ser Gly Arg Ile Lys Lys Glu Glu Phe Ala G1u Ile Met Lys atc tgt tcc acc att gaa gag ctc aga cgg caa aaa taatgaattt 2305 Ile Cys Ser Thr Ile Glu Glu Leu Arg Arg Gln Lys agcttgtcct tcatgaaaaa atgccttgtt tctact 2341 <210> 4 <211> 757 <212> PRT
<213> Influenza A virus <400> 4 Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His Gly Thr Gly Thr Gly Tyr Thr Met Asp Thr Val Asn Arg Thr His Gln 35 40 ~ 45 Tyr Ser Glu Lys Gly Lys Trp Thr Thr Asn Thr Glu Thr Gly Ala Pro Gln Leu Asn Pro Ile Asp Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser Gly Tyr Ala Gln Thr Asp Cys Val Leu Glu Ala Met Ala Phe Leu Glu Glu Ser His Pro Gly Ile Phe Glu Asn Ser Cys Leu Glu Thr Met Glu Val Val Gln Gln Thr Arg Val Asp Arg Leu Thr'Gln Gly Arg Gln Thr Tyr Asp Trp Thr Leu Asn Arg Asn Gln Pro Ala Ala Thr Ala Leu Ala A5n Thr Ile Glu Val Phe Arg Ser Asn Gly Leu Thr Ala Asn Glu Ser Gly Arg Leu Ile Asp Phe Leu Lys Asp Val Met Glu Ser Met Asp Lys Glu Glu Met Glu Ile Thr Thr His Phe Gln Arg Lys Arg Arg Val Arg Asp Asn Met Thr Lys Lys Met Val Thr Gln Arg Thr Ile Gly Lys Lys Lys Gln Arg Val Asn Lys Arg Ser Tyr Leu Ile Arg Ala Leu Thr Leu Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala Ile Ala Thr Pro Gly Met Gln Ile Arg Gly Phe Val Tyr Phe Val Glu Thr Leu Ala Arg Ser Ile Cys Glu Lys Leu Glu Gln Ser Gly Leu Pro Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ala Asn Val Val Arg Lys Met Met Thr Asn Ser Gln Asp Thr Glu Leu Ser Phe Thr Ile Thr Gly Asp Asn Thr Lys Trp Asn Glu Asn Gln Asn Pro Arg Met Phe Leu Ala Met Ile Thr Tyr Ile Thr Lys Asn Gln Pro Glu Trp Phe Arg Asn Ile Leu Ser Ile Ala Pro Ile Met Phe Ser Asn Lys Met Ala Arg Leu Gly Lys Gly Tyr Met Phe Glu Ser Lys Arg Met Lys Leu Arg Thr Gln Ile Pro Ala Glu Met Leu Ala Ser Ile Asp Leu Lys Tyr Phe Asn Glu Ser Thr Arg Lys Lys Ile Glu Lys Ile Lys Pro Leu Leu Ile Asp Gly Thr Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu Ser Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Lys Tyr Thr Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly Val Asp Arg Phe Tyr Arg Thr Cys Lys Leu Val Gly Ile Asn Met Ser Lys Lys Lys Ser Tyr Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser Phe Phe Tyr Arg Tyr Gly Phe Val Ala Asn Phe Ser Met Glu Leu Pro Ser Phe Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Met Ser Ile Gly Val Thr Val Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala Thr Ala Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr Tyr Arg Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe Glu Leu Lys Lys Leu Trp Glu Gln Thr Arg Ser Lys Ala Gly Leu Leu Val Ser Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile Pro Glu Va1 Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly Arg Leu 610' 615 620 Cys Asn Pro Leu Asn Pro Phe Val Ser His Lys Glu Ile Glu Ser Val Asn Asn Ala Val Val Met Pro Ala His Gly Pro Ala Lys Ser Met Glu Tyr Asp Ala Val Ala Thr Thr His Ser Trp Ile Pro Lys Arg Asn Arg Ser Ile Leu Asn Thr Ser Gln Arg Gly Ile Leu Glu Asp Glu Gln Met Tyr Gln Lys Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser Ser Ser Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met Val Ser Arg Ala Arg Ile Asp Ala Arg Ile Asp Phe Glu Ser Gly Arg Ile Lys Lys Glu Glu Phe Ala Glu Ile Met Lys Ile Cys Ser Thr Ile Glu Glu Leu Arg Arg Gln Lys <210> 5 <211> 2233 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (25)..(2172) <400> 5 agcaaaagca ggtactgatt cgag atg gaa gat ttt gtg cga caa tgc ttc 51 Met Glu Asp Phe Val Arg Gln Cys Phe aat ccg atg att gtc gag ctt gca gaa aag gca atg aaa gag tat gga 99 Asn Pro Met Ile Val Glu Leu Ala Glu Lys Ala Met Lys Glu Tyr Gly gag gat ctg aaa atc gaa aca aac aaa ttt gca gca ata tgc act cac 147 Glu Asp Leu Lys Ile Glu Thr Asn Lys Phe Ala Ala Ile Cys Thr His ttg gag ata tgt ttc atg tat tca gat ttt cat ttc atc aat gaa caa 195 Leu Glu Ile Cys Phe Met Tyr Ser Asp Phe His Phe Ile Asn Glu Gln ggc gag tca ata gtg gta gag ctt gat gat cca aat gca ctg tta aag 243 Gly Glu Ser Ile Val Val Glu Leu Asp Asp Pro Asn Ala Leu Leu Lys cac aga ttt gaa ata ata gag gga aga gac cgc aca atg gcc tgg aca 291 His Arg Phe Glu Ile Ile Glu Gly Arg Asp Arg Thr Met Ala Trp Thr gta gta aac agt att tgc aac act act gga get gag aaa ccg aag ttt 339 Val Val Asn Ser Ile Cys Asn Thr Thr Gly Ala Glu Lys Pro Lys Phe 90~ 95 100 105 ctg cca gat ttg tat gat tac aag gag aat aga ttc atc gag att gga 387 Leu Pro Asp Leu Tyr Asp Tyr Lys Glu Asn Arg Phe Ile Glu Ile Gly gtg aca agg aga gaa gtc cac ata tac tac ctt gaa aag gcc aat aaa 435 Val Thr Arg Arg Glu Val His Ile Tyr Tyr Leu Glu Lys Ala Asn Lys att aaa tct gag aat aca cac atc cac att ttc tca ttc act ggg gag 483 Ile Lys Ser Glu Asn Thr His Ile His Ile Phe Ser Phe Thr Gly Glu gaa atg gcc aca aag gcc gac tac act ctt gat gat gaa agc agg get 531 Glu Met Ala Thr Lys Ala Asp Tyr Thr Leu Asp Asp Glu Ser Arg Ala agg atc aaa acc agg cta ttt acc ata aga caa gaa atg gcc aac aga 579 Arg Ile Lys Thr Arg Leu Phe Thr Ile Arg Gln Glu Met Ala Asn Arg ggC CtC tgg gat tcc ttt cgt cag tcc gaa aga ggc gaa gaa aca att 627 Gly Leu Trp Asp Ser Phe Arg Gln Ser Glu Arg Gly Glu Glu Thr Ile gaa gaa aga ttt gaa atc aca gga act atg cgc agg ctt gcc gac caa 675 Glu Glu Arg Phe Glu Ile Thr Gly Thr Met Arg Arg Leu Ala Asp Gln 205 210 ' 215 agt ctc ccg ccg aac ttc tcc tgc ctt gag aat ttt aga gcc tat gtg 723 Ser Leu Pro Pro Asn Phe Ser Cys Leu Glu Asn Phe Arg Ala Tyr Val gat gga ttc gaa ccg aac ggc tgc att gag ggc aag ctt tct caa atg 771 Asp Gly Phe Glu Pro Asn Gly Cys Ile Glu Gly Lys Leu Ser Gln Met tcc aaa gaa gtg aat gca aga att gaa cct ttt ctg aag aca aca cca 819 Ser Lys Glu Val Asn Ala Arg Ile Glu Pro Phe Leu Lys Thr Thr Pro aga cca atc aaa ctt ccg gat ggg cct cct tgt ttt cag cgg tcc aaa 867 Arg Pro Ile Lys Leu Pro Asp Gly Pro Pro Cys Phe Gln Arg Ser Lys ttc ctt ctg atg gat get tta aaa tta agc att gaa gac cca agt cac 915 Phe Leu Leu Met Asp Ala Leu Lys Leu Ser Ile Glu Asp Pro Ser His gaa gga gag gga ata cca cta tat gat gcg atc aag tgc atg aga aca 963 Glu Gly Glu Gly Ile Pro Leu Tyr Asp Ala Ile Lys Cys Met Arg Thr ttc ttt gga tgg aaa gaa ccc tat atc gtc aaa cca cac gaa aag gga 1011 Phe Phe Gly Trp Lys Glu Pro Tyr Ile Val Lys Pro His Glu Lys Gly ata aat cca aat tat ctg ctg tca tgg aag caa gta ctg gca gaa cta 1059 Ile Asn Pro Asn Tyr Leu Leu Ser Trp Lys Gln Val Leu Ala Glu Leu cag gac att gaa aat gag gag aag att cca aga act aaa aac atg aag 1107 Gln Asp Ile Glu Asn Glu Glu Lys Ile Pro Arg Thr Lys Asn Met Lys aaa acg agt cag cta aag tgg gca ctt ggt gag aac atg gca cct gag 1155 Lys Thr Ser Gln Leu Lys Trp Ala Leu Gly Glu Asn Met Al.a Pro Glu aaa gta gac ttt gac aac tgt aga gac ata agc gat ttg aag caa tat 1203 Lys Val Asp Phe Asp Asn Cys Arg Asp Ile Ser Asp Leu Lys Gln Tyr gat agt gac gaa cct gaa tta agg tca ctt tca agc tgg atc cag aat 1251 Asp Ser Asp Glu Pro Glu Leu Arg Ser Leu Ser Ser Trp Ile Gln Asn gag ttc aac aag gca tgc gag ctg acc gat tca atc tgg ata gag ctc 1299 Glu Phe Asn Lys Ala Cys Glu Leu Thr Asp Ser Ile Trp Ile Glu Leu gat gag att gga gaa gac gtg get cca att gaa tac att gca agc atg 1347 Asp Glu Ile Gly Glu Asp Val Ala Pro Ile Glu Tyr Ile Ala Ser Met agg agg aat tac ttc aca gca gag gtg tcc cat tgc aga gcc aca gaa 1395 Arg Arg Asn Tyr Phe Thr Ala Glu Val Ser His Cys Arg Ala ~'hr Glu tac ata atg aag ggg gta tac att aat act gcc ttg ctt aat gca tcc 1443 Tyr Ile Met Lys Gly Val Tyr Ile Asn Thr Ala Leu Leu Asn Ala Ser tgt gca gca atg gac gat ttc caa cta att ccc atg ata agc aag tgc 1491 Cys Ala Ala Met Asp Asp Phe Gln Leu Ile Pro Met Ile Ser Lys Cys aga act aaa gag gga agg cga aaa acc aat tta tat gga ttc atc ata 1539 Arg Thr Lys Glu Gly Arg Arg Lys Thr Asn Leu Tyr Gly Phe Il'e Ile 490 ' 495 500 505 aaa gga aga tct cac tta agg aat gac acc gac gtg gta aac ttt gtg 1587 Lys Gly Arg Ser His Leu Arg Asn Asp Thr Asp Val Val Asn Phe Val agc atg gag ttt tct ctc act gac ccg aga ctt gag cca cat aaa tgg 1635 Ser Met Glu Phe Ser Leu Thr Asp Pro Arg Leu Glu Pro His Lys Trp gag aaa tac tgt gtc ctt gag ata gga gat atg cta cta aga agt gcc 1683 Glu Lys Tyr Cys Val Leu Glu Ile Gly Asp Met Leu Leu Arg Ser Ala ata ggc cag atg tca agg cct atg ttc ttg tat gtg agg aca aat gga 1731 Ile Gly Gln Met Ser Arg Pro Met Phe Leu Tyr Val Arg Thr Asn Gly aca tca aag att aaa atg aaa tgg gga atg gag atg aga cgt tgc ctc 1779 Thr Ser Lys Ile Lys Met Lys Trp Gly Met Glu Met Arg Arg Cys Leu ctt cag tca ctc caa caa atc gag agc atg att gaa gcc gag tct tct 1827 Leu Gln Ser Leu Gln Gln Ile Glu Ser Met Ile Glu Ala Glu Ser Ser gtc aaa gag aaa gac atg acc aaa gag ttt ttt gag aat aaa tca gaa 1875 Val Lys Glu Lys Asp Met Thr Lys Glu Phe Phe Glu Asn Lys Ser Glu aca tgg ccc att ggg gag tcc ccc aag gga gtg gaa gaa ggt tcc att 1923 Thr Trp Pro Ile Gly Glu Ser Pro Lys Gly Val Glu Glu Gly Ser Ile gga aag gtc tgt agg act tta ttg gcc aag tcg gta ttc aat agc ctg 1971 Gly Lys Val Cys Arg Thr Leu Leu Ala Lys Ser Val Phe Asn Ser Leu tat gca tcc cca caa ttg gaa gga ttt tca gcg gag tca aga aaa ctg 2019 Tyr Ala Ser Pro Gln Leu Glu Gly Phe Ser Ala Glu Ser Arg Lys Leu 650 655 660 ~ 665 ctt ctt gtc gtt cag get ctt agg gac aac ctt gaa cct gga acc ttt 2067 Leu Leu Val Val Gln Ala Leu Arg Asp Asn Leu Glu Pro Gly Thr Phe gat ctt ggg ggg cta tat gaa gca att gag gag tgc ctg att aat gat 2115 Asp Leu Gly Gly Leu Tyr Glu Ala Ile Glu Glu Cys Leu Ile Asn Asp ccc tgg gtt ttg ctt aat gcg tct tgg ttc aac tcc ttc cta aca cat 2163 Pro Trp Val Leu Leu Asn Ala Ser Trp Phe Asn Ser Phe Leu Thr His gca tta aga tagttgtggc aatgctacta tttgctatcc atactgtcca 2212 Ala Leu Arg aaaaagtacc ttgtttctac t 2233 <210> 6 <211> 716 <212> PRT
<213> Influenza A virus <400> 6 Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Ile Cys Phe Met Tyr Ser Asp Phe His Phe Ile Asn Glu Gln Gly Glu Ser Ile Val Val Glu Leu Asp Asp Pro Asn A1a Leu Leu Lys His Arg Phe Glu Ile Ile Glu Gly Arg Asp Arg Thr Met Ala Trp Thr Val Val Asn Ser Ile Cys Asn Thr Thr Gly Ala Glu Lys-Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr Lys Glu Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Asn Thr His Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp Tyr Thr Leu Asp Asp Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe Thr Ile Arg Gln Glu Met Ala Asn Arg Gly Leu Trp Asp Ser Phe Arg Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr Gly Thr Met Arg Arg Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser Cys Leu Glu Asn Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly Cys Ile Glu Gly Lys Leu Ser Gln Met Ser Lys.Glu Val Asn Ala Arg Ile Glu Pro Phe Leu Lys Thr Thr Pro Arg Pro Ile Lys Leu Pro Asp Gly Pro Pro Cys Phe Gln Arg Ser Lys Phe Leu Leu Met Asp Ala Leu Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro Tyr Ile Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Asn Cys Arg Asp Ile Ser Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu Arg Ser Leu Ser Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu Leu Thr Asp Ser Ile Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Val Ala Pro Ile Glu Tyr Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg Lys Thr, Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu Ile Gly Asp Met Leu Leu Arg Ser Ala Ile Gly Gln Met Ser Arg Pro Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Ile Gly Glu Ser Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Val Val Gln Ala Leu Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg <210> 7 <211> 1765 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (30)..(1727) <400> 7 agcaaaagca ggggataatt ctattaacc atg aag act atc att get ttg agc 53 Met Lys Thr Ile Ile Ala Leu Ser tac att ttc tgt ctg gtt ctc ggc caa aac ttt cca gga aat gac aac 101 Tyr Ile Phe Cys Leu Val Leu Gly Gln Asn Phe Pro Gly Asn Asp Asn agc aca gca acg ctg tgc ctg gga cat cat gcg gtg cca aac gga aca 149 Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr cta gtg aaa aca atc aca aat gat cag att gaa gtg act aat get act 197 Leu Val Lys Thr Ile Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr gag ctg gtt cag agt tcc tca acg ggg aaa ata tgc aac aat cct cat 245 Glu Leu Val Gln Ser Ser Ser Thr Gly Lys Ile Cys Asn Asn Pro His cga atc ctt gat gga ata gac tgc aca ctg ata gat get cta ttg ggg 293 Arg Ile Leu Asp Gly Ile Asp Cys Thr Leu Ile Asp Ala Leu Leu Gly gac cct cat tgt gat ggc ttt caa aat gag aca tgg gac ctt ttc gtt 341 Asp Pro His Cys Asp Gly Phe Gln Asn Glu Thr Trp Asp Leu Phe Val gaa cgc agc aaa get ttc agc aac tgt tac cct tat gat gtg cca gat 389 Glu Arg Ser Lys Ala Phe Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp tat gcc tcc ctt agg tca cta gtt gcc tcg tca ggc act ctg gag ttt 437 Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe atc agt gaa ggc ttc act tgg act ggg gtc act cag aat ggg gga agc 485 Ile Ser Glu Gly Phe Thr Trp Thr Gly Val Thr Gln Asn Gly Gly Ser aat get tgc aaa agg gga cct gat agc ggt ttt ttc agt aga ctg aac 533 Asn Ala Cys Lys Arg Gly Pro Asp Ser Gly Phe Phe Ser Arg Leu Asn tgg ttg tac aaa tca gga agc aca tat cca gtg ctg aac gtg act atg 581 Trp Leu Tyr Lys Ser Gly Ser Thr Tyr Pro Val Leu Asn Val Thr Met cca aac aat gac aat ttt gac aaa cta tac att tgg ggg gtt cac cac 629 Pro Asn Asn Asp Asn Phe Asp Lys Leu Tyr Ile Trp Gly Val His His ceg agc acg gac caa gaa caa acc agc cta tat gtt caa gca tca ggg 677 Pro Ser Thr Asp Gln Glu Gln Thr Ser Leu Tyr Val Gln Ala Ser Gly aga gtc aca gtc tct acc aag aga agc cag caa act ata atc ccg aat 725 Arg Val Thr Val Ser Thr Lys Arg Ser Gln Gln Thr Ile Ile Pro Asn atc ggg tct aga ccc tgg gta agg ggt ctg tct agt aga ata agc atc 773 Ile Gly Ser Arg Pro Trp Val Arg Gly Leu Ser Ser Arg Ile Ser Ile tat tgg aca ata gtt aaa ccg gga gac ata ctg gta att aat agt aat 821 Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Val Ile Asn Ser Asn ggg aac cta att get cct cgg ggt tat ttt aaa atg cgc act ggg aaa 869 Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Met Arg Thr Gly Lys agc tca ata atg agg tca gat gca cct att ggc acc tgc att tct gaa 917 Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Thr Cys Ile Ser Glu tgc atc act cca aat gga agc att ccc aat gac aag ccc ttt caa aac 965 Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn gta aac aag atc aca tat ggg gca tgt ccc aag tat gtt aag caa aac 1013 Val Asn Lys Ile Thr Tyr Gly Ala Cys Pro Lys Tyr Val Lys Gln Asn acc ctg aag ttg gca aca ggg atg cgg aat gta cca gag aaa caa act 1061 Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr aga ggc cta ttc agc gca ata gca ggt ttc ata gaa aat ggg tgg gag 1109 Arg Gly Leu Phe Ser Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu gga atg ata gac ggt tgg tac ggt ttc agg cat caa aat tct gag ggc 1157 Gly Met Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly aca gga caa gca gca gat ctt aaa agc act caa gca gcc atc gac caa 1205 Thr Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln atc aat ggg aaa ctg aat agg gta atc gag aag acg aac gag aaa ttc 1253 Ile Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Phe cat caa atc gaa aag gaa ttc tca, gaa gta gaa ggg aga att cag gac 1301 His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp ctc gag aaa tac gtt gaa gac act aaa ata gat ctc tgg tct tac aat 1349 Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn gcg gag ctt ctt gtc get ctg gag aac caa cat aca att gat ctg act 1397 Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr gac tcg gaa atg aac aaa ctg ttt gaa aaa aca agg agg caa ctg agg 1445 Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Arg Gln Leu Arg gaa aat get gag gac atg ggc aat ggt tgc ttc aaa ata ttc cac aaa 1493 Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Phe His Lys tgt gac aat get tgc ata ggg tca atc aga aat ggg act tat gac cat 1541 Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His gat gta tac aga gac gaa gca tta aac aac cgg ttt cag atc aaa ggt 1589 Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly gtt gaa ctg aag tca gga tac aaa aac tgg atc ctg tgg att tcc ttt 1637 Val Glu Leu Lys Ser Gly Tyr Lys Asn Trp Ile Leu Trp Ile Ser Phe gcc ata tca tgc ttt ttg ctt tgt gtt gtt ttg ctg ggg ttc atc atg 1685 Ala Ile Ser Cys Phe Leu Leu Cys Val Val Leu Leu Gly Phe Ile Met tgg gcc tgc cag aaa ggc aac att agg tgc aac att tgc att 1727 Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile tgagtgtatt agtaattaaa aacacccttg tttctact 1765 <210> 8 <211> 566 <212> PRT
<213> Influenza A virus <400> 8 Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Leu Gly Gln Asn Phe Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr Gly Lys Ile Cys Asn Asn Pro His Arg Ile Leu Asp Gly Ile Asp Cys Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln Asn Glu Thr Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Phe~Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe Ile Ser Glu Gly Phe Thr Trp Thr Gly Val Thr Gln Asn Gly Gly Ser Asn Ala Cys Lys Arg Gly Pro Asp Ser Gly Phe Phe Ser Arg Leu Asn Trp Leu Tyr Lys Ser Gly Ser Thr Tyr Pro Val Leu Asn Val Thr Met Pro Asn Asn Asp Asn Phe Asp Lys Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Gln Glu Gln Thr Ser Leu Tyr Val Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg Ser Gln Gln Thr Ile Ile Pro Asn Ile Gly Ser Arg Pro Trp Val Arg Gly Leu Ser Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly 245 250 ~ 255 Asp Ile Leu Val Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Met Arg Thr Gly Lys Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Thr Cys Ile Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala Cys Pro Lys Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Leu Phe Ser Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser 405 4l0 415 Glu Val Glu Gly Arg I1e Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Arg Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Phe His Lys Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys Asn Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile <210> 9 <211> 1565 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (46)..(1539) <400> 9 agcaaaagca gggttaataa tcactcactg agtgacatca aaatc atg gcg tcc caa 57 Met Ala Ser Gln ggc acc aaa cgg tct tat gaa cag atg gaa act gat ggg gaa cgc cag 105 Gly Thr Lys Arg Ser Tyr Glu Gln Met Glu Thr Asp Gly Glu Arg Gln aat gca act gag atc agg gca tcc gtc ggg aag atg att gat gga att 153 Asn Ala Thr Glu Ile Arg Ala Ser Val Gly Lys Met Ile Asp Gly Ile gga cga ttc tac atc caa atg tgc act gaa ctt aaa ctc agt gat tat 201 Gly Arg Phe Tyr Ile Gln Met Cys Thr Glu Leu Lys Leu Ser Asp Tyr gag ggg cga ttg atc cag aac agc tta aca ata gag aga atg gtg ctc 249 Glu Gly Arg Leu Ile Gln Asn Ser Leu Thr Ile Glu Arg Met Val Leu tct get ttt gat gag aga agg aat aga tat ctg gaa gaa cat ccc agc 297 Ser Ala Phe Asp Glu Arg Arg Asn Arg Tyr Leu Glu Glu His Pro Ser gcg ggg aaa gat cct aag aaa act gga ggg ccc ata tac aag aga gta 345 Ala Gly Lys Asp Pro Lys Lys Thr Gly Gly Pro Ile Tyr Lys Arg Val gat aga aag tgg atg agg gaa ctc gtc ctt tat gac aaa gaa gaa ata 393 Asp Arg Lys Trp Met Arg Glu Leu Val Leu Tyr Asp Lys Glu Glu Ile agg cga atc tgg cgc caa gcc aat aat ggt gat gat gcg aca get ggt 441 Arg Arg Ile Trp Arg Gln Ala Asn Asn Gly Asp Asp Ala Thr Ala Gly cta acc cac atg atg atc tgg cat tcc aat ttg aat gat aca aca tac 489 Leu Thr His Met Met Ile Trp His Ser Asn Leu Asn Asp Thr Thr Tyr cag agg aca aga get ctt gtt cgc acc gga atg gat ccc agg atg tgc 537 Gln Arg Thr Arg Ala Leu Val Arg Thr Gly Met Asp Pro Arg Met Cys tct ctg atg cag ggt tcg act ctc cct agg agg tct gga get gca ggc~ 585 Ser Leu Met Gln Gly Ser Thr Leu Pro Arg Arg Ser Gly Ala Ala Gly get gca gtc aaa gga gtc ggg aca atg gtg atg gag ctg atc aga atg 633 Ala Ala Val Lys Gly Val Gly Thr Met Val Met Glu Leu Ile Arg Met atc aaa cgt ggg atc aac gat cgg aac ttc tgg aga ggt gag aat gga 681 Ile Lys Arg Gly Ile Asn Asp Arg Asn Phe Trp Arg Gly Glu Asn Gly cga aaa aca agg ggt get tat gag aga atg tgc aac att ctc aaa gga 729 Arg Lys Thr Arg Gly Ala Tyr Glu Arg Met Cys Asn Ile Leu Lys Gly aaa ttt caa aca get gca caa aga gca atg atg gat caa gtg aga gaa 777 Lys Phe Gln Thr Ala Ala Gln Arg Ala Met Met Asp Gln Val Arg Glu 230 ' 235 240 agc cgg aac cca gga aat get gag atc gaa gat ctc ata ttt ttg gca 825 Ser Arg Asn Pro Gly Asn Ala Glu Ile Glu Asp Leu Ile Phe Leu Ala cgg tct gca cta ata ttg aga ggg tca gtt get cac aaa tct tgt ctg 873 Arg Ser Ala Leu Ile Leu Arg Gly Ser Val Ala His Lys Ser Cys Leu cct gcc tgt gtg tat gga cct gcc gta gcc agt ggg tac gac ttc gaa 921 Pro Ala Cys Val Tyr Gly Pro Ala Val Ala Ser Gly Tyr Asp Phe Glu aaa gag gga tac tct tta gtg gga ata gac cct ttc aaa cta ctt caa 969 Lys Glu Gly Tyr Ser Leu Val Gly Ile Asp Pro Phe Lys Leu Leu Gln aac agc caa gta tac agc cta atc aga ccg aac gag aat cca gca cac 1017 Asn Ser Gln Val Tyr Ser Leu Ile Arg Pro Asn Glu Asn Pro Ala His aag agt cag ctg gtg tgg atg gca tgc aat tct get gca ttt gaa gat 1065 Lys Ser Gln Leu Val Trp Met Ala Cys Asn Ser Ala Ala Phe Glu Asp cta aga tta tta agc ttc atc aga ggg acc aaa gta tct cca agg ggg 1113 Leu Arg Leu Leu Ser Phe Ile Arg Gly Thr Lys Val Ser Pro Arg Gly aaa ctt tca act aga gga gta caa att get tca aat gaa aac atg gat 1161 Lys Leu Ser Thr Arg Gly Va1 Gln Ile Ala Ser Asn Glu Asn Met Asp act atg gaa tca agt act ctt gaa ctg aga agc agg tac tgg gcc ata 1209 Thr Met Glu Ser Ser Thr Leu Glu Leu Arg Ser Arg Tyr Trp Ala Ile agg acc aga agt gga gga aac act aat caa cag agg gcc tcc gca ggc 1257 Arg Thr Arg Ser Gly Gly Asn Thr Asn Gln Gln Arg Ala Ser Ala Gly caa atc agt gtg caa cct gca ttt tct gtg caa aga aac ctc cca ttt 1305 Gln Ile Ser Val Gln Pro Ala Phe Ser Val Gln Arg Asn Leu Pro Phe gac aaa tca acc atc atg gca gca ttc act ggg aat acg gag gga aga 1353 Asp Lys Ser Thr Ile Met Ala Ala Phe Thr Gly Asn Thr Glu Gly Arg acc tca gac atg agg gca gaa atc ata agg atg atg gaa ggt gca aaa 1401 Thr Ser Asp Met Arg Ala Glu Ile Ile Arg Met Met Glu Gly Ala Lys cca gaa gaa gtg tcc ttc cgt ggg cgg gga gtc ttc gag ctc tcg gac 1449 Pro Glu Glu Val Ser Phe Arg Gly Arg Gly Val Phe Glu Leu Ser Asp gaa aag gca acg aac ccg atc gtg ccc tct ttt gac atg agt aat gaa 1497 Glu Lys Ala Thr Asn Pro Ile Val Pro Ser Phe Asp Met Ser Asn Glu gga tct tat ttc ttc gga gac aat gca gag gag tac gac aat 1539 Gly Ser Tyr Phe Phe Gly Asp Asn Ala Glu Glu Tyr Asp Asn taaggaaaaa tacccttgtt tctact 1565 <210> 10 <211> 498 <212> PRT
<213> Influenza A virus <400> 10 Met Ala Ser Gln Gly Thr Lys Arg Ser Tyr Glu Gln Met Glu Thr Asp 1 5 10 . 15 Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val Gly Lys Met Ile Asp Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr Glu Leu Lys Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu Thr Ile Glu Arg Met Va1 Leu Ser Ala Phe Asp Glu Arg Arg Asn Arg Tyr Leu Glu Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly Gly Pro Ile Tyr Lys Arg Val Asp Arg Lys Trp Met Arg Glu Leu Val Leu Tyr Asp Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn Gly Asp Asp Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser Asn Leu Asn Asp Thr Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr Gly Met Asp Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro Arg Arg Ser Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met Val Met Glu Leu Ile Arg Met Ile Lys Arg Gly Ile Asn Asp Arg Asn Phe Trp Arg Gly Glu Asn Gly Arg Lys Thr Arg Gly Ala Tyr Glu Arg Met Cys Asn Ile Leu Lys Gly Lys Phe Gln Thr Ala Ala Gln Arg Ala Met Met Asp Gln Val Arg Glu Ser Arg Asn Pro Gly Asn Ala Glu Tle Glu Asp Leu Ile Phe Leu Ala Arg Ser Ala Leu Ile Leu Arg Gly Ser Val Ala His Lys Ser Cys Leu Pro Ala Cys Val Tyr Gly Pro Ala Val Ala Ser Gly Tyr Asp Phe Glu Lys Glu Gly Tyr Ser Leu Val Gly Ile Asp Pro Phe Lys Leu Leu Gln Asn Ser Gln Val Tyr Ser Leu Ile Arg Pro Asn Glu Asn Pro Ala His Lys Ser Gln Leu Val Trp Met Ala Cys Asn Ser Ala Ala Phe Glu Asp Leu Arg Leu Leu Ser Phe Ile Arg Gly Thr Lys Val Ser Pro Arg Gly Lys Leu Ser Thr Arg Gly Val Gln Ile Ala Ser Asn Glu Asn Met Asp Thr Met Glu Ser Ser Thr Leu Glu Leu Arg Ser Arg Tyr Trp Ala Ile Arg Thr Arg Ser Gly Gly Asn Thr Asn Gln Gln Arg Ala Ser Ala Gly Gln Ile Ser Val Gln Pro Ala Phe Ser Val Gln Arg Asn Leu Pro Phe Asp Lys Ser Thr Ile Met Ala Ala Phe Thr Gly Asn Thr Glu Gly Arg Thr Ser Asp Met Arg Ala Glu Ile Ile Arg Met Met Glu Gly Ala Lys Pro Glu Glu Val Ser Phe Arg Gly Arg Gly Val Phe Glu Leu Ser Asp Glu Lys Ala Thr Asn Pro Ile Val Pro Ser Phe Asp Met Ser Asn Glu Gly Ser Tyr Phe Phe Gly Asp Asn Ala Glu Glu Tyr Asp Asn <210> 11 <211> 1466 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (20)..(1426) <400> 11 agcaaaagca ggagtgaag atg aat cca aat caa aag ata ata aca att ggc 52 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly tct gtc tct ctc acc att gca aca ata tgc ttc ctc atg cag att gcc 100 Ser Val Ser Leu Thr Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala atc ctg gta act act gta aca ttg cat ttc aag caa tat gag tgc gac 148 Ile Leu Val Thr Thr Val Thr Leu His Phe Lys Gln Tyr Glu Cys Asp tcc ccc gcg aac aac caa gta atg ccg tgt gaa cca ata ata ata gaa 196 Ser Pro Ala Asn Asn Gln Val Met Pro Cys Glu Pro Ile Ile Ile Glu aggaac ataacagag atagtgtat ttgactaac accaccata gagaaa 244 ArgAsn IleThrGlu IleValTyr LeuThrAsn ThrThrIle GluLys.

gagata tgccccaaa ttagtggaa tacaggaat tggtcaaag ccgcaa 292 GluIle CysProLys LeuValGlu TyrArgAsn TrpSerLys ProGln tgtaaa attacagga tttgcacct ttttctaag gacaattca attcgg 340 CysLys IleThrGly PheAlaPro PheSerLys AspAsnSer IleArg ctttct getggtggg gacatttgg gtgacgaga gaaccttat gtgtca 388 LeuSer AlaGlyG1y AspIleTrp ValThrArg GluProTyr ValSer tgcgat cctggcaag tgttatcaa tttgcactc gggcagggg accaca 436 CysAsp ProGlyLys CysTyrGln PheAlaLeu GlyGlnGly ThrThr ctagac aacaaacat tcaaatgac acaatacat gatagaacc cctcat 484 LeuAsp AsnLysHis SerAsnAsp ThrIleHis AspArgThr ProHis cgaacc ctattgatg aatgagttg ggtgttcca tttcatttg ggaacc 532 ArgThr LeuLeuMet AsnGluLeu GlyValPro PheHisLeu GlyThr aggcaa gtgtgtata gcatggtcc agctcaagt tgtcacgat ggaaaa 580 ArgGln ValCysIle AlaTrpSer SerSerSer CysHisAsp GlyLys gca tgg ctg cat gtt tgt gtc act ggg tat gat aaa aat gca act get 628 Ala Trp Leu His Val Cys Val Thr Gly Tyr Asp Lys Asn Ala Thr Ala agc ttc att tac gat ggg agg ctt gta gac agt att ggt tca tgg tct 676 Ser Phe Ile Tyr Asp Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser caa aat atc ctc agg acc cag gag tcg gaa tgc gtt tgt atc aat ggg 724 Gln Asn Ile Leu Arg Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly act tgt aca gta gta atg act gat gga agt get tca gga aga get gat 772 Thr Cys Thr Val Val Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp act aaa ata cta ttc att gaa gag ggg aaa att gtt cat att agc cca 820 Thr Lys Ile Leu Phe Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro ttg tca gga agt get cag cat gta gag gag tgt tcc tgt tat cct cga 868 Leu Ser Gly Ser Ala Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg tat'cct ggt gtc aga tgt atc tgc aga gac aac tgg aaa ggc tct aat 916 Tyr Pro Gly Val Arg Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn agg ccc gtc gta gat ata aat gtg aaa gat tat agc att gat tcc agt 964 Arg Pro Val Val Asp Ile Asn Val Lys Asp Tyr Ser Ile Asp Ser Ser tat gtg tgc tca ggg ctt gtt ggc gac gca ccc aga aac aac gac aga 1012 Tyr Val Cys Ser Gly Leu Val Gly Asp Ala Pro Arg Asn Asn Asp Arg tct agc aat agc tat tgc cgg aat cct aac aat gag aaa ggg aat cac 1060 Ser Ser Asn Ser Tyr Cys Arg Asn Pro Asn Asn Glu Lys Gly Asn His gga gtg aaa ggc tgg gcc ttt gac gat gga aat gac gtg tgg atg gga 1108 Gly Val Lys Gly Trp Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly aga acg atc agc gag ga.t tca cgc tca ggt tat gaa acc ttc aaa gtc 1156 Arg Thr Ile Ser Glu Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val att ggt ggt tgg tcc aca cct aat tcc aaa ttg cag ata aat agg caa 1204 Ile Gly Gly Trp Ser Thr Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln gtc ata gtt gac agc gat aat agg tca ggt tat tct ggt att ttc tct 1252 Val Ile Val Asp Ser Asp Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser gtt gag ggc aaa agc tgc atc aat agg tgc ttt tat gtg gag ttg ata 1300 Val Glu Gly Lys Ser Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile agg gga agg gaa cag gaa act aga gta tgg tgg acc tca aac agt att 1348 Arg Gly Arg Glu Gln Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile gtt gtg ttt tgt ggc act tca ggt acc tat ggg aca ggc tca tgg cct 1396 Val Val Phe Cys Gly Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro gat ggg gcg gac atc aat ctc atg cct ata taagctttcg caattttaga 1446 Asp Gly Ala Asp Ile Asn Leu.Met Pro Ile aaaaactcct tgtttctact 1466 <210> 12 <211> 469 <212> PRT
<213> Influenza A virus <400> 12 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr Val Thr Leu His Phe Lys Gln Tyr Glu Cys Asp Ser Pro Ala Asn Asn Gln Va1 Met Pro Cys Glu Pro Ile Ile Ile Glu Arg Asn Ile Thr Glu Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys Leu Val Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Lys Ile Thr Gly Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly Asp.Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Gly Lys Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Lys His Ser Asn Asp Thr Ile His Asp Arg Thr Pro His Arg Thr Leu Leu Met Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val Cys Val Thr Gly Tyr Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asp Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Ser Gly Ser Ala Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Val Val Asp Ile Asn Val Lys Asp Tyr Ser Ile Asp Ser Ser Tyr Val Cys Ser Gly Leu Val Gly Asp Ala Pro Arg Asn Asn Asp Arg Ser Ser Asn Ser Tyr Cys Arg Asn Pro Asn Asn Glu Lys Gly Asn His Gly Val Lys Gly Trp Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Glu Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Gly Gly Trp Ser Thr Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Ser Asp Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Glu Gln Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile Asn Leu Met Pro Ile <210> 13 <211> 1027 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (26)..(781) <400> 13 agcaaaagca ggtagatatt gaaag atg agc ctt ctg acc gag gtc gaa acg 52 Met Ser Leu Leu Thr Glu Val Glu Thr tat gtt ctc tct atc gtt ccg tca ggc ccc ctc aaa gcc gag atc gcg 100 Tyr Val Leu Ser Ile Val Pro Ser Gly Pro Leu Lys Ala Glu Ile Ala cag aga ctt gaa gat gtc ttt get gga aag aac aca gat ctt gag get 148 Gln Arg Leu Glu Asp Val Phe Ala Gly Lys Asn Thr Asp Leu Glu Ala ctc atg gaa tgg cta aag aca aga cca atc ctg tca cct ctg act aaa 196 Leu Met Glu Trp Leu Lys Thr Arg Pro Ile Leu Ser Pro Leu Th.r Lys ggg att ttg gga ttt gta ttc acg ctc acc gtg ccc agt gag cga gga 244 Gly Ile Leu Gly Phe Val Phe Thr Leu Thr Val Pro Ser Glu Arg Gly ctg cag cgt aga cgc ttt gtc caa aat gcc ctc aat ggg aat ggg gat 292 Leu Gln Arg Arg Arg Phe Val Gln Asn Ala Leu Asn Gly Asn Gly Asp cca aat aac atg gac aga gca gtt aaa ctg tat aga aaa ctt aag agg 340 Pro Asn Asn Met Asp Arg Ala Val Lys Leu Tyr Arg Lys Leu Lys Arg gag ata aca ttc cat ggg gcc aaa gaa ata gca ctc agt tat tct gct, 388 Glu Ile Thr Phe His Gly Ala Lys Glu Ile Ala Leu Ser Tyr Ser Ala ggt gca ctt gcc agt tgc atg ggc ctc ata tac aac agg atg ggg get 436 Gly Ala Leu Ala Ser Cys Met Gly Leu Ile Tyr Asn Arg Met Gly Ala gtg acc act gaa gtg gcc ttt ggc ctg gtt tgt gca acc tgt gaa cag 484 Val Thr Thr Glu Val Ala Phe Gly Leu Val Cys Ala Thr Cys Glu Gln att get gac tcc cag cac agg tct cat agg caa atg gtg gca aca acc 532 Ile Ala Asp Ser Gln His Arg Ser His Arg Gln Met Val Ala Thr Thr aat cca cta ata aga cat gag aac aga atg gtt ctg gcc agc act aca 580 Asn Pro Leu Ile Arg His Glu Asn Arg Met Val Leu Ala Ser Thr Thr get aag get atg gag caa atg get gga tca agt gag cag gca gca gag 628 Ala Lys Ala Met Glu Gln Met Ala Gly Ser Ser Glu Gln Ala Ala Glu gcc atg gag gtt get agt cag gcc agg caa atg gtg cag gca atg aga 676 Ala Met Glu Val Ala Ser Gln Ala Arg Gln Met Val Gln Ala Met Arg gcc att ggg act cat cct agc tcc agt get ggt cta aaa gat gat ctt 724 Ala Ile Gly Thr His Pro Ser Ser Ser Ala Gly Leu Lys Asp Asp Leu ctt gaa aat ttg cag gcc tat cag aaa cga atg ggg gtg cag atg caa 772 Leu Glu Asn Leu Gln Ala Tyr Gln Lys Arg Met Gly Val Gln Met Gln cga ttc aag tgaccctctt gttgttgctg cgagtatcat tgggatcttg 821 Arg Phe Lys cacttgatat tgtggattct tgatcgtctt tttttcaaat gcatctatcg attctttgaa 881 cacggtctga aaagagggcc ttctacggaa ggagtacctg agtctatgag ggaagaatat 941 cgaaaggaac agcagagtgc tgtggatgct gacgacagtc attttgtcag catagagctg 1001 gagtaaaaaa ctaccttgtt tctact 1027 <210> 14 <211> 252 <212> PRT
<213> Influenza A virus <400> 14 Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Val Pro Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe Ala Gly Lys Asn Thr Asp Leu Glu Ala Leu Met Glu Trp Leu Lys Thr Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Arg Arg Arg Phe Val Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Arg Ala Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala Lys Glu Ile Ala Leu Ser Tyr Ser Ala Gly Ala Leu Ala Ser Cys Met Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg Ser His Arg Gln Met Val Ala Thr Thr Asn Pro Leu Ile Arg His Glu Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Glu Val Ala Ser Gln Ala Arg Gln Met Val Gln Ala Met Arg Ala Ile Gly Thr His Pro Ser Ser Ser Ala Gly Leu Lys Asp Asp Leu Leu Glu Asn Leu Gln Ala Tyr Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys <210> 15 <211> 322 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (26)..(316) <400> 15 agcaaaagca ggtagatatt gaaag atg agc ctt ctg acc gag gtc gaa acg 52 Met Ser Leu Leu Thr Glu Val Glu Thr cct atc aga aac gaa tgg ggg tgc aga tgc aac gat tca agt gac cct 100 Pro Ile Arg Asn Glu Trp Gly Cys Arg Cys Asn Asp Ser Ser Asp Pro ctt gtt gtt get gcg agt atc att ggg atc ttg cac ttg ata ttg tgg 148 Leu Val Va1 Ala Ala Ser Ile Ile Gly Ile Leu His Leu Ile Leu Trp att ctt gat cgt ctt ttt ttc aaa tgc atc tat cga ttc ttt gaa cac 196 Ile Leu Asp Arg Leu Phe Phe Lys Cys Ile Tyr Arg Phe Phe Glu His ggt ctg aaa aga ggg cct tct acg gaa gga gta cct gag tct atg agg 244 Gly Leu Lys Arg Gly Pro Ser Thr Glu Gly Val Pro Glu Ser Met Arg gaa gaa tat cga aag gaa cag cag agt get gtg gat get gac gac agt 292 Glu Glu Tyr Arg Lys Glu Gln Gln Ser Ala Val Asp Ala Asp Asp Ser cat ttt gtc agc ata gag ctg gag taaaaa 322 His Phe Val Ser Ile Glu Leu Glu <210> 16 <211> 97 <212> PRT
<213> Influenza A virus <400> 16 Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Cys Arg Cys Asn Asp Ser Ser Asp Pro Leu Val Val Ala Ala Ser Ile 20 ~ 25 30 Ile Gly Ile Leu His Leu Ile Leu Trp Ile Leu Asp Arg Leu Phe Phe Lys Cys Ile Tyr Arg Phe Phe Glu His Gly Leu Lys Arg Gly Pro Ser Thr Glu Gly Val Pro Glu Ser Met Arg Glu Glu Tyr Arg Lys Glu Gln Gln Ser Ala Val Asp Ala Asp Asp Ser His Phe Val Ser Ile Glu Leu Glu <210> 17 <211> 890 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (27)..(737) <400> 17 agcaaaagca gggtgacaaa gacata atg gat tcc aac act gtg tca agt ttt 53 Met Asp Ser Asn Thr Val Ser Ser Phe cag gta gac tgc ttc ctt tgg cat gtc cga aaa caa gtt gta gac caa 101 Gln Val Asp Cys Phe Leu Trp His Val Arg Lys Gln Val Val Asp Gln gaa cta ggt gat gcc cca ttc ctt gat cgg ctt cgc cga gat cag aag 149 Glu Leu Gly Asp Ala Pro Phe Leu Asp Arg Leu Arg Arg Asp Gln Lys tcc cta agg gga aga ggc agc act ctc ggt cta aac atc gaa gca gcc 197 Ser Leu Arg Gly Arg Gly Ser Thr Leu Gly Leu Asn Ile Glu Ala Ala acc cat gtt gga aag cag ata gta gag aag att ctg aag gaa gaa tct 245 Thr His Val Gly Lys Gln Ile Val Glu Lys Ile Leu Lys Glu Glu Ser gat gag gca ctt aaa atg acc atg gcc tcc aca cct get tcg cga tac 293 Asp Glu Ala Leu Lys Met Thr Met Ala Ser Thr Pro Ala Ser Arg Tyr '75 80 85 ata act gac atg act att gag gaa ttg tca agg gac tgg ttc atg cta 341 Ile Thr Asp Met Thr Ile Glu Glu Leu Ser Arg Asp Trp Phe Met Leu atg ccc aag cag aaa gtg gaa gga cct ctt tgc atc aga ata gac caa 389 Met Pro Lys Gln Lys Val Glu Gly Pro Leu Cys Ile Arg Ile Asp Gln gca atc atg gat aag aac atc atg ttg aaa gcg aat ttc agt gtg att 437 Ala Ile Met Asp Lys Asn Ile Met Leu Lys Ala Asn Phe Ser Val Ile ttt gac cgg cta gag acc cta ata tta cta agg get ttc acc gaa gag 485 Phe Asp Arg Leu Glu Thr Leu Ile Leu Leu Arg Ala Phe Thr Glu Glu gga gca att gtt ggc gaa atc tca cca ttg cct tct ttt cca gga cat 533 Gly Ala Ile Val Gly Glu Ile Ser Pro Leu Pro Ser Phe Pro Gly His act att gag gat gtc aaa aat gca att ggg gtc ctc atc gga gga ctt 581 Thr Ile Glu Asp Val Lys Asn Ala Ile Gly Val Leu Ile Gly Gly Leu gaa tgg aat gat aac aca gtt cga gtc tct aaa act cta cag aga ttc 629 Glu Trp Asn Asp Asn Thr Val Arg Val Ser Lys Thr Leu Gln Arg Phe get tgg gga agc agt aat gag aat ggg aga cct cca ctt act cca aaa 677 Ala Trp Gly Ser Ser Asn Glu Asn Gly Arg Pro Pro Leu Thr Pro Lys cag aaa cgg aaa atg gcg aga aca get agg tca aaa gtt cga aga gat 725 Gln Lys Arg Lys Met Ala Arg Thr Ala Arg Ser Lys Val Arg Arg Asp aag atg get gat tgaagaagtg agacacagac tgaagacaac agagaatagt 777 Lys Met Ala Asp tttgagcaaa taacattcat gcaagcctta cagctactat ttgaagtgga acaggagata 837 agaactttct cgtttcagct tatttaatga taaaaaacac ccttgtttct act 890 <210> 18 <211> 237 <212> PRT
<213> Influenza A virus <400> 18 Met Asp Ser Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp His Val Arg Lys Gln Val Val Asp Gln Glu Leu Gly Asp Ala Pro Phe Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser Thr Leu Gly Leu Asn Ile Glu Ala Ala Thr His Val Gly Lys Gln Ile Val Glu Lys Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr Met Ala Ser Thr Pro Ala Ser Arg Tyr Ile Thr Asp Met Thr Ile Glu Glu Leu Ser Arg Asp Trp Phe Met Leu Met Pro Lys Gln Lys Val Glu G1y Pro Leu Cys Ile Arg Ile Asp Gln Ala Ile Met Asp Lys Asn Ile Met Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile Ser Pro Leu Pro Ser Phe Pro Gly His Thr Ile Glu Asp Val Lys Asn Ala Ile Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val Arg Val Ser Lys Thr Leu Gln Arg Phe Ala Trp Gly Ser Ser Asn Glu Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Lys Met Ala Arg Thr Ala Arg Ser Lys Val Arg Arg Asp Lys Met Ala Asp <210> 19 <211> 402 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (27)..(389) <400> 19 agcaaaagca gggtgacaaa gacata atg gat tcc aac act gtg tca agt ttt 53 Met Asp Ser Asn Thr Va1 Ser Ser Phe cag gac ata cta ttg agg atg tca aaa atg caa ttg ggg tcc tca tcg 101 Gln Asp Ile Leu Leu Arg Met Ser Lys Met Gln Leu Gly Ser Ser Ser gag gac ttg aat gga atg ata aca cag ttc gag tct cta aaa ctc tac 149 Glu Asp Leu Asn Gly Met Ile Thr Gln Phe Glu Ser Leu Lys Leu Tyr aga gat tcg ctt ggg gaa gca gta atg aga atg gga gac ctc cac tta 197 Arg Asp Ser Leu Gly Glu Ala Val Met Arg Met Gly Asp Leu His Leu ctc caa aac aga aac gga aaa tgg cga gaa cag cta ggt caa aag ttc 245 Leu Gln Asn Arg Asn Gly Lys Trp Arg Glu Gln Leu Gly Gln Lys Phe gaa gag ata aga tgg ctg att gaa gaa gtg aga cac aga ctg aag aca 293 Glu Glu Ile Arg Trp Leu Ile Glu Glu Val Arg His Arg Leu Lys Thr aca gag aat agt ttt gag caa ata aca ttc atg caa gcc tta cag cta 341 Thr Glu Asn Ser Phe Glu Gln Ile Thr Phe Met Gln Ala Leu Gln Leu cta ttt gaa gtg gaa cag gag ata aga act ttc tcg ttt cag ctt att 389 Leu Phe Glu Val Glu Gln Glu Ile Arg Thr Phe Ser Phe Gln Leu Ile taatgataaa aaa 402 <210> 20 <211> 121 <212> PRT
<213> Influenza A virus <400> 20 Met Asp Ser Asn Thr Val Ser Ser Phe Gln Asp Ile Leu Leu Arg Met Ser Lys Met Gln Leu Gly Ser Ser Ser Glu Asp Leu Asn Gly Met Ile Thr Gln Phe Glu Ser Leu Lys Leu Tyr Arg Asp Ser Leu Gly Glu Ala Val Met Arg Met Gly Asp Leu His Leu Leu Gln Asn Arg Asn Gly Lys 50. 55 60 Trp Arg Glu Gln Leu Gly Gln Lys Phe Glu Glu Ile Arg Trp Leu Ile Glu Glu Val Arg His Arg Leu Lys Thr Thr Glu Asn Ser Phe Glu Gln Ile Thr Phe Met Gln Ala Leu Gln Leu Leu Phe Glu Val Glu Gln Glu Ile Arg Thr Phe Ser Phe Gln Leu Ile <210> 21 <211> 1764 <212> DNA
<213> Influenza A virus <220>
<221> CDS
<222> (30)..(1727) <400> 21 agcaaaagca ggggataatt ctattaacc atg aaa act atc att get ttg agc 53 Met Lys Thr Ile Ile Ala Leu Ser tac att ttc tgt ctg gtt ctc ggc caa gac ttt cca gga aat gac aac 101 Tyr Ile Phe Cys Leu Val Leu Gly Gln Asp Phe Pro Gly Asn Asp Asn agc aca gca acg ctg tgc ctg gga cat cat gcg gtg cca aac gga aca 149 Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr cta gtg aaa aca atc aca aat gat cag att gaa gtg act aat get act 197 Leu Val Lys Thr Ile Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr gag ctg gtt cag agt tcc tca acg ggg aaa ata tgc aac aat cct cat 245 Glu Leu Val Gln Ser Ser Ser Thr Gly Lys Ile Cys Asn Asn Pro His cga atc ctt gat gga ata gac tgc aca ctg ata gat get cta ttg ggg 293 Arg Ile Leu Asp Gly Ile Asp Cys Thr Leu Ile Asp Ala Leu Leu Gly gac cct cat tgt gat ggc ttt caa aat gag aca tgg gac ctt ttc gtt 341 Asp Pro His Cys Asp Gly Phe Gln Asn Glu Thr Trp Asp Leu Phe Val gaa cgc agc aaa get ttc agc aac tgt tac cct tat gat gtg cca gat 389 Glu Arg Ser Lys Ala Phe Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp tat gcc tCC Ctt agg tca cta gtt gcc tcg tca ggc act ctg gag ttt 437 Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe atc agt gaa ggc ttc act tgg act ggg gtc act cag aat ggg gga agc 485 Ile Ser Glu Gly Phe Thr Trp Thr Gly Val Thr Gln Asn Gly Gly Ser aat get tgc aaa agg gga cct gat agc ggt ttt ttc agt aga ctg aac 533 Asn Ala Cys Lys Arg Gly Pro Asp Ser Gly Phe Phe Ser Arg Leu Asn tgg ttg tac aaa tca gga agc aca tat cca gtg ctg aac gtg act atg 581 Trp Leu Tyr Lys Ser Gly Ser Thr Tyr Pro Val Leu Asn Val Thr Met cca aac aat gac aat ttt gac aaa cta tac att tgg ggg gtt cac cac 629 Pro Asn Asn Asp Asn Phe Asp Lys Leu Tyr Ile Trp Gly Val His His ccg agc acg gac caa gaa caa acc agc cta tat gtt caa gca tca ggg 677 Pro Ser Thr Asp Gln Glu Gln Thr Ser Leu Tyr Val Gln Ala Ser Gly aga gtc aca gtc tct acc aag aga agc cag caa act ata atc ccg aat 725 Arg Val Thr Val Ser Thr Lys Arg Ser Gln Gln Thr Ile Ile Pro Asn atc ggg tct aga ccc tgg gta agg ggt ctg tct agt aga ata agc atc 773 Ile Gly Ser Arg Pro Trp Val Arg Gly Leu Ser Ser Arg Ile Ser Ile tat tgg aca ata gtt aaa ccg gga gac ata ctg .gta att aat agt aat 821 Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Val Ile Asn Ser Asn ggg aac cta att get cct cgg ggt tat ttt aaa atg cgc act ggg aaa 869 Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Met Arg Thr Gly Lys agc tca ata atg agg tca gat gca cct att ggc acc tgc att tct gaa 917 Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Thr Cys Ile Ser Glu tgc atc act cca aat gga agc att ccc aat gac aag ccc ttt caa aac 965 Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn gta aac aag atc aca tat ggg gca tgt ccc aag tat gtt aag caa aac 1013 Val Asn Lys Ile Thr Tyr Gly Ala Cys Pro Lys Tyr Val Lys Gln Asn acc ctg aag ttg gca aca ggg atg cgg aat gta cca gag aaa caa act 1061 Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr aga ggc cta ttc agc gca ata gca ggt ttc ata gaa aat ggt tgg gag 1109 Arg Gly Leu Phe Ser Ala lle Ala Gly Phe Ile Glu Asn Gly Trp Glu gga atg ata gac ggt tgg tac ggt ttc agg cat caa aat tct gag ggc 1157 Gly Met Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly aca gga caa gca gca gat ctt aaa agc act caa gca gcc atc gac caa 1205 Thr Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln atc aat ggg aaa ctg aat agg gta atc gag aag acg aac gag aaa ttc 1253 Ile Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Phe cat caa atc gaa aag gaa ttc tca gaa gta gaa ggg aga att cag gac 1301 His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp ctc gag aaa tac gtt gaa gac act aaa ata gat ctc tgg tct tac aat 1349 Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn gcg gag ctt ctt gtc get ctg gag aac caa cat aca att gat ctg act 1397 Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr gac tcg gaa atg aac aaa ctg ttt gaa aaa aca agg agg caa ctg agg 1445 Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Arg Gln Leu Arg gaa aat get gag gac atg ggc aat ggt tgc ttc aaa ata tac cac aaa 1493 Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys tgt gac aat get tgc ata ggg tca atc aga aat ggg act tat gac cat 1541 Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His gat gta tac aga gac gaa gca tta aac aac cgg ttt cag atc aaa ggt 1589 Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 505 510 5'15 520 gtt gaa ctg aag tca gga tac aaa gac tgg atc ctg tgg att tcc ttt 1637 Val Glu Leu.Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe gcc ata tca tgc ttt ttg ctt tgt gtt gtt ttg ctg ggg ttc atc atg 1685 Ala Bile Ser Cys Phe Leu Leu Cys Val Val Leu Leu Gly Phe Ile Met tgg gcc tgc cag aaa ggc aac att agg tgc aac att tgc att 1727 Trp~Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile tgagtgtatt agtaattaaa aacaccctgt ttctact 1764 ..
<210> 22 <211> 566 <212> PRT
<213> Influenza A virus <400> 22 Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Leu Gly Gln Asp Phe Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr Gly Lys Ile Cys Asn Asn Pro His Arg Ile Leu Asp Gly Ile Asp Cys Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln Asn Glu Thr Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Phe Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe Ile Ser Glu Gly Phe Thr Trp Thr Gly Val Thr Gln Asn Gly Gly Ser Asn Ala Cys Lys Arg Gly Pro Asp Ser Gly Phe Phe Ser Arg Leu Asn Trp Leu Tyr Lys Ser Gly Ser Thr Tyr Pro Val Leu Asn Val Thr Met Pro Asn Asn Asp Asn Phe Asp Lys Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Gln Glu Gln Thr Ser Leu Tyr Val Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg Ser Gln Gln Thr Ile Ile Pro Asn Ile Gly Ser Arg Pro Trp Val Arg Gly Leu Ser Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Val Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Met Arg Thr Gly Lys Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Thr Cys Ile Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala Cys Pro Lys Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Leu Phe Ser Ala,Ile Ala Gly Phe Ile G1u Asn Gly Trp Glu Gly Met Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Arg Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile <210> 23 <211> 27 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 23 tatggaaaga ataaaagaac tacggaa 27 <210> 24 <211> 22 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 24 tcgtttttaa actattcaac at 22 <210> 25 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 25 agcaggtcaa ttatattcaa tatg 24 <210> 26 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence:Primer , <400> 26 aacaaggtcg tttttaaact attc 24 <210> 27 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 27 agaactctat tccaacaaat g 21 <210> 28 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 28 aatcggatat ttcattgcca t 21 <210> 29 <211> 33 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 29 agaactctat tccaacaaat gagggatgta gtt 33 <210> 30 <211> 33 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 30 aatcggatat ttcattgcca tcatccattt cat 33 <210> 31 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 31 agacgtggtg ttggtaatga a 21 <210> 32 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 32 cgaagagttg acataaaccc t 21 <210> 33 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 33 tcatccctca tcccctcaca t 21 <210> 34 <211> 22 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 34 attttctgtt atcctcttgt ca 22 <210> 35 <211> 20 <212> DNA a <213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 35 gtggagtccg ctgttttgag 20 <210> 36 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 36 aggatggtgg acattcttag g 21 <210> 37 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 37 ccgatcaatg ctaaccacta c 21 <210> 38 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 38 agcaaaagca ggtcaattat attca 25 <210> 39 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 39 agtagaaaca aggtcgtttt taaac , 25 <210> 40 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 40 agcaaaagca ggcaaaccat 20 <210> 41 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 41 agtagaaaca aggcattttt t 21 <210> 42 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 42 tcgagctgaa gaagctatgg 20 <210> 43 <211> 20 <2l2> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 43 gttctgttga ctgtgtccat 20 <210> 44 <211> 32 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 44 tcgagctgaa gaagctatgg gagcagaccc gt 32 <210> 45 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 45 gttctgttga ctgtgtccat ggtgtatcc 29 <210> 46 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 46 agcgaaagca ggcaaaccat ttgaatggat gtcaa 35 <210> 47 <211> 31 <212> DNA

<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 47 attaaaaaca aggcattttt tcatgaagga c 31 <210> 48 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 48 aatttccagc atggtggagg ccatggtg 28 <210> 49 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 49 gggatctttg aaaactcgtg 20 <210> 50 <211> 19 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 50 cagcattgtt tacagactc 19 <210> 51 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 51 accaaagatg cagaaagagg 20 <210> 52 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 52 ctcatattga ttccgactaa 20 <210> 53 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 53 tgtCClCttC CCtttttCtg a 21 <210> 54 <211> 22 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 54 atttttcccc agtagttcat ac 22 <210> 55 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 55 acgctgttgc aactacacac tcctg 25 <210> 56 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 56 agcaaaagca ggtactgatt cgaga 25 <210> 57 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 57 agtagaaaca aggtactttt ttgga 25 <210> 58 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 58 gaacctggaa cctttgatct t 21 <210> 59 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 59 attcaccact gtccaggcca t 21 <210> 60 <211> 30 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 60 gaacctggaa cctttgatct tgaggggcta 30 <210> 61 <211> 30 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 61 attcaccact gtccaggcca ttgacgcgtc 30 <210> 62 <211> 21 <212> DNA
<213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Primer <400> 62 agcaaaagca ggtagtgata g 21 <220> 63 <211> 15 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 63 agtagaaaca aggta 15 <210> 64 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: primer <400> 64 agcgaaagca ggtagtgatt cgagatgga 29 <210> 65 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 65 agtagaaaca aggtactttt ttggacag 28 <210> 66 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 66 tcgatttgtt ggagtgactg a 21 <210> 67 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 67 gccgaacttc tcctgccttg a 21 <210> 68 <211> 19 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 68 gtattcaata gcctgtatg 19 <210> 69 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 69 gacttctctc cttgtcactc 20 <210> 70 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 70 acgagtcagc taaagtgggc a 21 <210> 71 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 71 gacacctctg ctgtgaagta a 21 <210> 72 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 72 ggagctgaga aaccgaagtt t 21 <210> 73 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 73 gacttggcca ataaagtcct a 21 <210> 74 <211> 21 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 74 gcctgcgcat agttcctgtg a ' 21 <210> 75 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 75 tctaccacta ttgactcgcc 20 <210> 76 <211> 23 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 76 agcaaaagca ggggataatt cta 23 <210> 77 <211> 23 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 77 agtagaaaca agggtgtttt taa 23 <210> 78 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 78 acagtttgtt catttccgag 20 <210> 79 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 79 acttcagggt gttttgctta ~ 20 <210> 80 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 80 gaacccccca aatgtatagt 20 <210> 81 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 81 tgaggaactc tgaaccagct 20 <210> 82 ' <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 82 gtcactagtt gcctcgtcag 20 <210> 83 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 83 ccgggagaca tactggtaat 20 <210> 84 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 84 caaatcaatg ggaaactgaa 20 <210> 85 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 85 tgaactgaag tcaggataca 20 <210> 86 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 86 agcaaaagca gggttaataa tcac 24 <210> 87 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 87 agtagaaaca agggtatttt tcct 24 <210> 88 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 88 ttgcaccttc catcatcctt 20 <210> 89 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 89 gtctattccc actaaagagt 20 <210> 90 <211> 20 <212 > DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 90 tgcatcagag agcacatcct 20 <210> 91 <211> 20 <212> DNA ' <213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 91 gaactcgtcc tttatgacaa 20 <210> 92 <211> 17 _ <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 92 agagcaatgg atcaagt 17 <210> 93 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 93 tactatggaa tcaagtactc . 20 <210> 94 <211> 18 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 94 atcaatcatc ttcccgac 18 <210> 95 <211> 18 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 95 agtgtccttc cgtgggcg ~ 18 <210> 96 <211> 23 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 96 agcaaaagca ggagtgaaga tga 23 <210> 97 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 97 agtagaaaca aggagttttt tcta 24 <210> 98 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 98 tcgttgtttc tgggtgtgtc 20 <210> 99 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 99 ttatcatacc cagtgacaca 20 <210> 100 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 100 attattattg gttcacacgg 20 <210> 101 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence:Primer <400> 101 ttatgtgtca tgcgatcctg 20 <210> 102 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 102 attgttcata ttagcccatt 20 <210> 103 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 103 ataggtcagg ttattctggt 20 <210> 104 <211> 15 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 104 agcaaaagca ggtag 15 <210> 105 <211> 15 ' <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 105 agtagaaaca aggta 15 <210> 106 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 106 tttactccag ctctatgctg acaa 24 <210> 107 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 107 gatccagcca tttgctccat 20 <210> 108 <211> 20 <212> DNA

<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 108 gaggtgacag gattggtctt 20 <210> 109 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 109 catggacaga gcagttaaac 20 <210> 110 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 110 gcgagtatca ttgggatctt 20 <210> 111 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 111 agcaaaagca gggtgacaaa gaca 24 <210> 112 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 112 agtagaaaca agggtgtttt ttat 24 <210> 113 <211> 24 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 113 ttttttatca ttaaataagc tgaa 24 <210> 114 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 114 aattgcattt ttgacatcct 20 <210> 115 <211> 20 <212> DNA
<213> Artificial Sequence <220> , <223> Description of Artificial Sequence: Primer <400> 115 atcttctcta ctatctgctt 20 <210> 116 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 116 caagcaatca tggataagaa 20 <210> 117 <211> 20 <212> DNA
<213> Artificial Sequence <220>
<223> Description of Artificial Sequence: Primer <400> 117 ggcgagaaca gctaggtcaa 20

Claims (20)

What is claimed is:

1. Isolated nucleic acid molecules which comprise the complete nucleotide sequence of the Influenza A/Udorn/72 (H3N2) strain in positive strand, antigenomic message sense, where said complete sequence consists of: (a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:17, or (b) SEQ ID NO:1, SEQ
ID NO:3, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ
ID NO:13, SEQ ID NO:17 and SEQ ID NO:21.

2. Isolated nucleic acid molecules of Claim 1 which comprise the complete nucleotide sequence of the Influenza A/Udorn/72 (H3N2) strain, where said complete sequence consists of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:17.

3. Isolated nucleic acid molecules of Claim 1 which comprise the complete nucleotide sequence of the Influenza A/Udorn/72 (H3N2) strain, where said complete sequence consists of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17 and SEQ ID NO:21.

4. An isolated nucleic acid molecule comprising an individual influenza A virus nucleotide sequence in positive strand, antigenomic message sense, where said sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11 and SEQ ID NO:21.

5. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:1.

6. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:3.
cattgccttt ttcttcatca tcaccacaac ca

7. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:5.

8. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:7.

9. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:11.

10. An isolated nucleic acid molecule of Claim 4, wherein the influenza A virus nucleotide sequence is SEQ ID NO:21.

11. An isolated nucleic acid molecule comprising an individual influenza A virus nucleotide sequence in positive strand, antigenomic message sense, where said sequence encodes a protein having a sequence selected from the group consisting of SEQ ID
NO:2, SEQ ID NO:4 and SEQ ID NO:6.

12. An isolated nucleic acid molecule of Claim 11, wherein the influenza A virus nucleotide sequence encodes a protein whose sequence is SEQ ID
NO:2.

13. An isolated nucleic acid molecule of Claim 11, wherein the influenza A virus nucleotide sequence encodes a protein whose sequence is SEQ ID
NO:4.

14. An isolated nucleic acid molecule of Claim 11, wherein the influenza A virus nucleotide sequence encodes a protein whose sequence is SEQ ID
NO:6.

15. An isolated amino acid sequence comprising an individual influenza A virus amino acid sequence, where said sequence is selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4 and SEQ
ID NO:6.

16. An isolated amino acid sequence of Claim 15, wherein the influenza A virus amino acid sequence is SEQ ID NO:2.

17. An isolated amino acid sequence of Claim 15, wherein the influenza A virus amino acid sequence is SEQ ID NO:4.

18. An isolated amino acid sequence of Claim 15, wherein the influenza A virus amino acid sequence is SEQ ID NO:6.

19. An isolated amino acid sequence comprising an individual influenza A virus sequence, where said sequence is SEQ ID NO:8.

20. An isolated amino acid sequence comprising an individual influenza A virus sequence, where said sequence is SEQ ID NO:12.