CN1437612A

CN1437612A - Nucleotide sequence of influenza A/Udorn/72(H3N2) genome

Info

Publication number: CN1437612A
Application number: CN01811649A
Authority: CN
Inventors: J·M·加拉尔萨; T·E·莱瑟姆
Original assignee: American Cyanamid Co
Current assignee: Wyeth Holdings LLC
Priority date: 2000-06-23
Filing date: 2001-06-21
Publication date: 2003-08-20
Also published as: WO2002000884A3; JP2004514415A; BR0111819A; MXPA02012255A; EP1292614A2; KR20030013451A; IL153005A0; AU2001270033A1; CA2410284A1; WO2002000884A2

Abstract

The complete nucleotide sequence of each of the eight segments of the negative strand Influenza A virus strain A/Udorn/72 (H3N2) is disclosed, which provides the complete sequence of the genome in antigenomic message sense. The sequence is useful in diagnosis and may be modified by mutation to generate new influenza A variant strains.

Description

The genomic nucleotide sequence of influenza A/Udorn/72 (H3N2)

Invention field

The present invention relates to the nucleotide sequence of each sections of influenza virus strains A/Udorn/72 (H3N2).

Background of invention

Influenza virus is made of the hypotype of called after A, B and C.Influenza A virus has single strand RNA genome of 8 sections, 10 peptide species (protein) that its viral life cycle of encoding is required.These proteinic orders are as follows: sections 12345678 protein PB2 PB1 PA HA NP NA M1 NS1 montage product M2 NS2

8 RNA sections of influenza A hypotype complete genome group each with the multiple subunit capsidation of nucleocapsid protein (NP), and combine with some molecules (PB1, PB2 and PA subunit) of three Polypolymerases, thereby form ribonucleoprotein mixture (RNP) (Lamb, R.A., " influenza virus " (The Influenza Viruses), 1-87 page or leaf, R.M.Krug are edited (Plenum Press, 1989)).NP albumen is structure and transcribes/duplicate adjusting albumen.As if these structure peripheries are round one deck stromatin (M1), and this albumen is the primary structure composition of virion and plays the nexus effect between nucleoid and peplos.This host cell deutero-coating is covered with the surface glycoprotein hemagglutinin (HA) of two kinds of main encoding virals and neuraminidase (NA) (these two kinds of surface glycoproteins are antigenic determinants) and a lot of less not glycosylated small protein M2 of quantity, and (Lamb 1989; Lamb, R.A. etc., Cell, 40,627-633 (1985)).M2 albumen is the montage gene product of 7# sections (the M1 albumen of also encoding), and it has ion channel activity; The montage gene only exists in the cell that infects.Similarly, unstructuredness NS2 albumen is the montage gene product of 8# sections (the unstructuredness NS1 albumen of also encoding); The montage gene only exists in the cell that infects.Proteolytic enzyme cutting HA glycoprotein forms HA1 and HA2.

Influenza infection is attached to by surperficial hemagglutinin and contains sialic cell receptor initiation.Reacting to each other for the first time of this virus-cell absorbs virion in the cell by receptor-mediated endocytosis.Under the low pH condition of endosome, HA has experienced hydrophobic NH2 stub area and the intermembranous interactional conformational change of endosome that promotes HA2, causes film to merge and subsequently core RNP and stromatin (M1) is discharged in the tenuigenin.Be transported to before the core of transcribing and duplicating that the complete genome group takes place at RNP, (Martin, K., and Helenius, A., Cell, 67, the 117-130 (1991) of dissociating of RNP and stromatin in tenuigenin, occur; Shapiro, G.I., etc., J.Virology, 61,764-773 (1987)).

After transcribing for the first time, new synthetic protein causes virus genomic duplicating, and synthesizes thereby increase to transcribe with albumen.In this stage of viral life cycle, surface glycoprotein HA and NA begin to assemble in the discrete areas of plasma membrane, and newly assembled virus then will discharge from plasma membrane.The assembling of supposing virus is that the tenuigenin of the structural protein by four kinds of encoding virals and/or some interaction of striding between diaphragm area are initial, these four kinds of albumen are: membrane anchor albumen (HA, NA and M2) and following stromatin (M1), stromatin is kept the tight (Garoff that is connected with RNP, H., Deng, " microbiology and molecular biology summary ", 62,1171-1190 (1998); Nayak, D.P., ASM News, 62,411-414 (1996)).So far also do not define contacting between matrix prote m1 and RNP mixture well, and the mechanism of mixing ripe virion of 8 kinds of RNP the whole series.How specific molecule contact domination form generating process starts and the process of ripe virion assembling and sprout from the surface of host cell between the putative structure composition.

In the process that viral genome is duplicated, there are a large amount of influenza A virus hypotypes and antigenicity variant to separate out continuously by the introducing sudden change of RNA polymerase.When they infect identical cell host, between the sections of two kinds of different subtypes, also undergo mutation by gene resortment.This biological characteristics of influenza A virus has produced new virus strains, thereby people just must be updated periodically the immunogenic composition of opposing influenza.Therefore, for the exploitation of novelty, the complete nucleotide sequence of a certain specific bacterial strain of influenza A virus and the validity of full-length clone must be arranged with the immunogenic composition of the opposing influenza of innovation.So, need to determine the complete nucleotide sequence of influenza A virus and make up the full-length clone that contains this sequence.

Specifically, separated the influenza A bacterial strain that is named as influenza A/Udorn/72 (H3N2) in 1972 first.So far do not measure the nucleotide sequence of pol gene PB2, PB1 and PA as yet.The nucleotide sequence of sections of coding HA, NP, M1, M2, NS1 and NS2 has checked order.Yet those that adopt many years ago that instrument obtains can not provide accurate sequence.Therefore, need repeat to check order so that up-to-date accurate sequence to be provided to these sections.

Summary of the invention

The objective of the invention is to determine the complete nucleotide sequence of influenza A/Udorn/72 (H3N2) virus strains and the montage product of 7# and 8# mRNA sections.Another object of the present invention is each clone and montage information of 8 kinds of genes of preparation.

Another object of the present invention is to determine the aminoacid sequence of deduction of all 10 kinds of products of these genes and montage information.

The nucleic acid molecule of the nucleotide sequence by illustrating all sections of isolating formation influenza A/Udorn/72 (H3N2) bacterial strain is finished these and other objects of the present invention, and these nucleic acid molecule comprise the complete nucleotide sequence of this bacterial strain and their montage information together.All these nucleotide sequences all show with normal chain, anti-genome (antigenomic) message sense.

Specifically, constitute complete nucleotide sequence by SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:17 and biology equivalent thereof.

Perhaps, complete nucleotide sequence comprises the variant of HA sequence, called after HA (P1) (SEQ IDNO:21), and described complete nucleotide sequence is made of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17 and SEQ ID NO:21 and biology equivalent thereof.

In another embodiment of the present invention, these isolating nucleic acid molecules are oriented to the nucleic acid molecule of each proteinic independent isolated influenza A virus of coding, and are selected from: SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11 and SEQ ID NO:21 (variant of SEQ ID NO:7) and biology equivalent thereof.

In another embodiment of the present invention, these independent isolated nucleic acid molecule codings have the protein of the aminoacid sequence that is selected from SEQ IDNO:2, SEQ ID NO:4 and SEQ ID NO:6 and biology equivalent thereof.

Another embodiment of the present invention provides the aminoacid sequence of independent isolated influenza A virus, and described sequence is selected from SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6 and biology equivalent thereof.

Another embodiment of the present invention provides independent influenza A virus aminoacid sequence, and described sequence has the sequence of SEQ ID NO:8 or SEQ ID NO:12.

In another embodiment of the present invention, use has the isolated nucleic acid molecule of the nucleotide sequence of influenza A/Udorn/72 (H3N2) bacterial strain sections: the primer of (1) design polymerase chain reaction (PCR), be used for pcr analysis, with existing of corresponding viral sections in the detection sample; Or (2) design and select to be used for the peptide of ELISA, with existing of the corresponding proteins matter that produces by this sections in the test sample.

Detailed Description Of The Invention

The nucleotide sequence of some sections of influenza A/Udorn/72 (H3N2) bacterial strain and the aminoacid sequence of deduction have been published.Yuferov etc. reported the HA gene of the 4th sections sequence (Yuferov, V.P., etc., Proceedings of the Academy of Sciences of the USSR, 278,738-742 (1984)).Buckler-White etc. have reported the sequence (Buckler-White, A.J., and Murphy, B.R., Virology, 155,345-355 (1986)) of the NP gene of the 5th sections.Markoff etc. have reported the sequence (Markoff, L., and Lai, C.J., Virology, 119,288-297 (1982)) of the NA gene of the 6th sections.Lamb etc. have reported the sequence of the M1 gene and the M2 gene splicing product of the 7th sections, and (M1 was at Lamb, R.A., and Lai, C.J.Virology, 112,746-751 (1981) (" Lamb Virology1981 ") in 1981; M2 is at Lamb, Proc.Natl.Acad.Sci.USA such as R.A., 78,4170-4174 (1981) (" Lamb PNAS 1981 ")).Lamb etc. have reported the sequence (Lamb, R.A., and Lai, C.J., Cell, 21,475-485 (1980)) of the NS1 gene and the NS2 gene splicing product of the 8th sections in 1980.

For these sections being resurveyed preface and those sections of not illustrating are as yet checked order, in Madin-Darby dog kidney (MDCK) cell, cultivate influenza A/Udorn/72 (H3N2) bacterial strain and purifying therefrom.Extract viral RNA from the virion of purifying, and carry out the RT-PCR amplification with terminal Auele Specific Primer.PGemT carrier (Promega) is gone in the gene clone of gel-purified, and check order with many cover primers.By 3 ' the segmental order-checking of RT-PCR that is connected and exchanges joint with 5 ', determine terminal sequence (Galarza, J.M. etc., J.Virol., 70,2360-2368 (1996)).M2 and NS2 gene are respectively the montage products of the mRNA of the 7th and the 8th sections.The mRNA of the cell purification that the MDCK-influenza A/Udorn that obtains from the RT-PCR that carries out with widow-dT and gene-specific primer infects reclaims these genes.RT-PCR product gel-purified, and be cloned into the pGemT carrier.Also determine all gene orders with fluorescence dye terminator and AmpliTaq archaeal dna polymerase (Perkin-Elmer) with Applied Biosystems ABI 377 dna sequencing instrument.Repeat this step and be used for each segmental a plurality of clone; The sequence that obtains is consistent.

The complete genome group of influenza A/Udorn/72 (H3N2) bacterial strain is 13628 Nucleotide altogether.This is a complete nucleotide sequence of describing influenza A/Udorn/72 (H3N2) bacterial strain first.

Measure (comprise and regulate sequence such as promotor, enhanser and polyadenylation signal) sequence of 8 sections at coding region, 5 ' and 3 ' non-coding region.Below be these sections, their Nucleotide quantity, their isolated nucleic acid molecule sequences (showing with normal chain, anti-genomic information chain, i.e. from 5 ' to 3 ' direction), their coding region and amino acid translation:

A sections 1#:2341 Nucleotide, SEQ ID NO:1, coding region Nucleotide 28-2304, coding PB2,759 amino acid (SEQ ID NO:2).

A sections 2#:2341 Nucleotide, SEQ ID NO:3, coding region Nucleotide 25-2295, coding PB1,757 amino acid (SEQ ID NO:4).

A sections 3#:2233 Nucleotide, SEQ ID NO:5, coding region Nucleotide 25-2172, coding PA, 716 amino acid (SEQ ID NO:6).

A sections 4#:1765 Nucleotide, SEQID NO:7, coding region Nucleotide 30-1727, coding HA, 566 amino acid (SEQ ID NO:8).

In some respects, Nucleotide and the aminoacid sequence of the HA that publishes such as SEQ ID NO:7 and SEQ ID NO:8 and Yuferov are different.

Yuferov SEQ ID NO:7 or 8

Codon # amino acid # codon # amino acid #

GAC????????????????Asp????????????????AAC???????????????Asn

81-83???????????????18????????????????81-83??????????????18

GGT????????????????Gly????????????????GGG???????????????Gly

1101-1103????????????358?????????????1101-1103????????????358

TAC????????????????Tyr????????????????TTC???????????????Phe

1485-1487????????????486?????????????1485-1487????????????486

GAC????????????????Asp????????????????AAC???????????????Asn

1614-1616????????????529?????????????1614-1616????????????529

A sections 5#:1565 Nucleotide, SEQ ID NO:9, coding region Nucleotide 46-1539, coding NP, 498 amino acid (SEQ ID NO:10).

Nucleotide and the aminoacid sequence of the NP that SEQ ID NO:9 and SEQ ID NO:10 and Buckler-White etc. publish are identical.

A sections 6#:1466 Nucleotide, SEQ ID NO:11, coding region Nucleotide 20-1426, coding NA, 469 amino acid (SEQ ID NO:12).

In some respects, Nucleotide and the aminoacid sequence of the NA that publishes such as SEQ ID NO:11 and SEQ ID NO:12 and Markoff are different.

Markoff SEQ ID NO:11 or 12

Codon # amino acid # codon # amino acid #

CAG??????????????Gln??????????????????CTG??????????????Leu

104-106?????????????29????????????????104-106?????????????29

GGA??????????????Gly??????????????????GCA??????????????Ala

546-548????????????177????????????????546-548????????????177

CAA??????????????Gln??????????????????CAG??????????????Gln

695-697????????????226????????????????695-697????????????226

ACA??????????????Thr??????????????????GCA??????????????Ala

992-994????????????325????????????????992-994????????????325

In amino acid/11, in the leader sequence upstream of methionine(Met) some differences are arranged also.

A sections 7#:1027 Nucleotide, SEQ ID NO:13, coding region Nucleotide 26-781, coding M1,252 amino acid (SEQ ID NO:14).

Nucleotide and the aminoacid sequence of M1 among SEQ ID NO:13 and SEQ ID NO:14 and the published Lamb Virology 1981 are identical.

Sections 7#, montage product: 322 Nucleotide, SEQ ID NO:15, coding region Nucleotide 26-316, coding M2,97 amino acid (SEQ ID NO:16) [amino acid of first montage at residue 10, Nucleotide 53-55].

Nucleotide and the aminoacid sequence of M2 among SEQ ID NO:15 and SEQ ID NO:16 and the published Lamb PNAS 1981 are identical.

A sections 8#:890 Nucleotide, SEQ ID NO:17, coding region Nucleotide 27-737, coding NS1,237 amino acid (SEQ ID NO:18).

Nucleotide and the aminoacid sequence of 1980 NS1 that publish such as SEQ ID NO:17 and SEQ ID NO:18 and Lamb are identical.

Sections 8#, montage product: 402 Nucleotide, SEQ ID NO:19, coding region Nucleotide 27-389, coding NS2,121 amino acid (SEQ ID NO:20) [amino acid of first montage at residue 11, Nucleotide 57-59].

Nucleotide and the aminoacid sequence of 1980 NS2 that publish such as SEQ ID NO:19 and SEQ ID NO:20 and Lamb are identical.

As mentioned above, another kind of HA sequence is arranged, called after HA (P1):

A sections 4#:1764 Nucleotide, SEQ ID NO:21, coding region Nucleotide 30-1727, coding HA, 566 amino acid (SEQ ID NO:22).

Do not wish to be bound by theory, As time goes on, may be in HA (P1) sequence in conjunction with a small amount of sudden change from influenza A/Udorn/72 (H3N2) bacterial strain.HA (P1) sequence (SEQ ID NO:21) has 1764 Nucleotide, and (SEQ ID NO:7) lacks a Nucleotide than the HA sequence, because a nucleotide deletion (1756) is arranged in the non-coding region of sections 4#.

The Nucleotide of two kinds of HA sequences of the present invention and aminoacid sequence have following difference:

Nucleotide site HA HA (P1) amino acid changes

35 G A silences

81 A G Asn become Asp

1103 G T silences

1486 T A Phe become Tyr

1614 A G Asn become Asp

1756 T disappearance is in the coding region outside

HA (P1) sequence also has following a little different with people's such as Yuferov HA nucleotide sequence: nucleotide site HA (P1) Yuferov amino acid changes

35 A G silences

1756 disappearance T coding region outsides

The nucleotide sequence of the mRNA of these sections, minus strand (promptly with 3 ' to 5 ' direction) are the complementations of above-mentioned normal chain, anti-genomic information chain-ordering in the genome.

Except that the nucleotide sequence of above-mentioned coding influenza A/Udorn/72 (H3N2) viral protein, the present invention also comprises the isolated nucleic acid molecule that contains each influenza A virus nucleotide sequence, because the Feng Yu of genetic code biologically is being equivalent to the sequence of those coding viral proteins, the feature that is other nucleotide sequence is different with nucleotide sequence as herein described, but the aminoacid sequence of its encoded protein matter with at SEQ ID NO:1, SEQ IDNO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11 is identical with arbitrary amino acid sequence coded among the SEQ ID NO:21.

Specifically, the present invention estimates that those are enough to Nucleotide and repeat arbitrary SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, the sequence of SEQ ID NO:11 and SEQ ID NO:21, thereby can under the strict Southern hybridization conditions of calibrated altitude, hybridize, as by described (Sambrook such as Sambrook, J., Deng, " molecular cloning: laboratory manual " (Molecular Cloning:A LabortoryManul), the 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)).

The present invention also comprises the nucleotide sequence of the coding aminoacid sequence different with the aminoacid sequence of influenza A/Udorn/72 (H3N2) viral protein, but its coded aminoacid sequence biologically with these viral proteins (SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6) in a kind of equivalence.If the difference of these aminoacid sequences and viral protein sequence only is the insertion in a spot of disappearance, the protein sequence or substitutes, thereby three grades of configurations comparing these sequences with the sequence of viral protein substantially change, just this aminoacid sequence is called with arbitrary viral protein be biologically equivalent.

For example, the codon of amino acid alanine (a kind of hydrophobic amino acid) the stronger residue (as Xie Ansuan, leucine or Isoleucine) of the more weak residue of another kind of hydrophobicity (as glycine) or hydrophobicity that can be encoded substitutes.Similarly, to cause a kind of electronegative (acidity) residue to substitute another kind of (substituting L-glutamic acid) as aspartic acid, or a kind of positively charged (alkalescence) residue substitutes another kind of (as Methionin place of arginine or Histidine), and based on the substituting of the similarity of residue in the hydrophilic index, expectation can produce the product of equivalence biologically.Expectation will cause the N-end of protein molecule or the nucleotide substitution of C-end parts variation also can not change activity of proteins.

The various variations of being advised are well known to those skilled in the art, and have determined the structure and the bioactive reservation of coded product.

The invention still further relates to isolating aminoacid sequence, it comprises each influenza A virus aminoacid sequence that is selected from SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6.

The invention still further relates to the aminoacid sequence of independent isolating SEQ ID NO:8 and SEQ ID NO:12.

The basic biological characteristics of influenza A/Udorn/72 (H3N2) virus depends on the genome that it is made of 8 sections.The sections of influenza virus is made of the strand of negative polarity RNA, and each viral chain has the specific nucleotide sequence of himself.

Therefore, complete difference is identified the nucleotide sequence that just must determine virus between virus strains.Owing to clearly determined that complete specificity nucleotide sequence is (as described herein) that is made of 13,628 bases, just can be on gene level identifying virus, therefore this authentication method provides absolute definite.

Sequence as herein described can be used for providing authentication method, and this method comprises a part that adopts polymerase chain reaction (PCR) method to detect the nucleotide sequence of the anti-genomic dna of influenza virus, and the preparation peptide is used for the antigen that ELISA detects the DNA generation.

Order-checking fully of concrete a kind of influenza virus and cloned genes group are favourable to the research and development based on the immunogenic composition of the novelty of this gene blueprint and innovation.

The sudden change of determining is introduced in required one or more sections of above-mentioned sequence.Introduce one or more sudden changes by directed mutagenesis.Yet, can not directly produce the mutant influenza virus from viral RNA, because genome virus RNA or anti-genome cDNA all can not be as the direct templates of synthetic.On the contrary, after with NP capsidation virus, must viral RNA be transcribed among the just mRNA by the viral rna polymerase mixture.

People such as Pales (U.S. Patent No. 5,166,057, this paper includes it as a reference in) have described the reverse genetics helper-dependent system that is used for " rescue " influenza A virus sections.Briefly, in the presence of three kinds of polymerase proteins and NP, by external synthetic preparation ribonucleoprotein (RNP) mixture.Then this RNP mixture is used for transfecting eukaryotic cells.Infect with influenza A helper virus subsequently, produce the virus that has derived from the gene of the cDNA sections of cloning.From a large amount of helper viruses, isolate required transfectant with system of selection then.

If save complete mutant influenza A bacterial strain, then use a kind of different system.Use Neumann etc. (Neumann, G., etc., Proc.Natl.Acad.Sci.USA, 96,9345-9350 (1999), this paper includes it as a reference in) described system, can prepare the influenza virus strains A/Udorn/72 (H3N2) of modified from clone's cDNA.This system based on plasmid does not need to use helper virus to infect.Briefly, with eight kinds of plasmids (the viral RNA sections of the bacterial strain of respectively encoding and flank are in suitable R NA polymerase promoter and terminator) and other four kinds of coding virus N P, PB2, PB1 and the proteic plasmids of PA (being used for synthesizing RNP in the cell in vivo) transfectional cell series, as human embryonic kidney cell line 293T.Adding in addition, five kinds of yield plasmids of expressing virus structural protein HA, NA, M1, M2 and NS2 significantly increase.

In the change of this system, only need eight kinds of plasmids (etc., Proc.Natl.Acad.Sci.USA, 97,6108-6113 (2000), this paper includes it as a reference in for Hoffmann, E.).Each plasmid comprises two kinds of promotors, human RNA polymerase I (pol I) promotor and human RNA polymerase II (pol II) promotor.After with eight kinds of expression plasmid transfecting eukaryotic cells, people pol I and II promotor are transcribed plasmid template respectively.This has caused the synthetic of virus mRNA and vRNA, thereby finally produces communicable influenza A virus.

Because any of 8,12 or 17 plasmid transfection systems do not need helper virus, so need not plaque purification with regard to recyclable transfectant virus.This system promotes the generation of attenuation influenza A virus alive, is used to resist the prevailing disease that comprises new HA or NA hypotype, wherein makes the sequence of the corresponding sections sudden change of influenza A/Udorn/72 (H3N2) bacterial strain with the coupling new subtype.

The sections (it is corresponding to the sequence of new circulation hypotype) of encode in influenza A/Udorn/72 (H3N2) bacterial strain HA or NA is introduced in the sudden change of adopting directed mutagenesis to be scheduled to.By the standard recombinant dna method this sudden change is introduced in the virus genomic DNA copy.

If desired, the corresponding sections with the susceptible toxic bacterial strain of various flows substitutes the HA of coding influenza A/Udorn/72 (H3N2) bacterial strain (or its mutant) or the sections of NA, generation permutatation virus.

In another embodiment of the present invention, with isolating nucleic acid molecule with nucleotide sequence of influenza A/Udorn/72 (H3N2) bacterial strain sections, prepare oligonucleotide probe (from the anti-genomic information of normal chain or from minus strand complementary gene group information) and express these peptides (only from the anti-genomic information of normal chain), can be used for detecting the existence of the sections of influenza A bacterial strain (or its mutant) in body fluid or the tissue sample.With the diagnostic test of nucleotide sequence design height specificity and susceptibility, with existing of viral sections in the test sample.

Based on virus sequence as herein described, with these sequence synthetic pcr primer things.Specimen is carried out the RNA reverse transcription, subsequently the selected cDNA zone of pcr amplification corresponding to nucleotide sequence described herein (have with this virus strains and determine the Nucleotide that sections is identical).On gel, identify amplification PCR products, and verify their specificity by hybridization with the specificity nucleotide probe.

Detect the proteinic existence that viral sections produces with the ELISA test.Design is also selected the peptide that comprises one or more different residues (based on virus sequence as herein described).Then these peptides are coupled to haptens (as keyhole limpet hemocyanin (KLH)) and are used for vaccine animal (as rabbit), to produce the monospecific polyclonal antibody.The combination of the selection of these polyclonal antibodies or polyclone and monoclonal antibody can be used for " catching ELISA " to detect the protein that this virus sections produces then.

This paper all includes the reference of being quoted as a reference in.

In order to allow the present invention be more readily understood, list following examples.These embodiment only are used for explanation, and scope of the present invention is not had any restriction.

Embodiment

Embodiment 1

The order-checking of influenza A/Udorn/72 (H3N2) bacterial strain

The following order-checking respectively of carrying out 8 sections:

The RT-PCR method

With Madin-Darby dog kidney (MDCK) cell culture influenza A/Udorn/72 (H3N2), and from clarifying culture supernatants, concentrate.Extract test kit (Qiagen) with RNAeasy and prepare genome virus RNA (vRNA) from 100 μ l virus stock solution useds.With Oligotex mRNA test kit (Qiagen), isolate the montage product (being respectively M2 and NS2) of the mRNA of sections 7 and 8 total mRNA of the mdck cell that infects from influenza A/Udorn.About 1 μ g vRNA or mRNA are added in the Eppendorf pipe of the water (to suppress the RNA enzyme) that 2pmol gene-specific primer and the processing of diethyl coke hydrochlorate are housed, to 12 μ l final volume.With widow-dT primer reverse transcription M2 and NS2 montage information.Heated the RNA-primer mixtures 10 minutes at 70 ℃, freezing rapidly on ice then.In this pipe, add following reagent: damping fluid (250mMTris-HCl pH 8.3,375mM KCl, the 15mM MgCl of 4 μ l 5X article one chains ₂), 2 μ l 0.1M DTT and 1 μ l dNTP mixture (respectively being 10mM dATP, dTTP, dGTP and dCTP), and 42 ℃ of cultivations 2 minutes.Subsequently, add 1 μ l SuperScript II (Gibco) reversed transcriptive enzyme, and cultivated again 50 minutes at 42 ℃.Cultivate 15 minutes termination RT reaction at 70 ℃.The above-mentioned RT of 2 μ l is added to beginning PCR reaction in the mixture of following reagent: 50mM Tris-HCl (pH 8.0), 1.5mM MgCl ₂, 50mM KCl, 1 each primer of μ M, each dNTP of 0.2mM and 2 Taq of unit polysaccharases.Following with Perkin-Elmer 9600 PCR instrument DNA amplification: 1 takes turns, 94 ℃ of sex change 2 minutes; 30 take turns, 94 ℃ of sex change 30 seconds; Annealed 30 seconds and extended 1 minute for 42 ℃ at 72 ℃.Carry out taking turns the extension some minutes at 72 ℃ then.On 1% sepharose, analyze the DNA of pcr amplification, and with Qiagen purification kit purifying.

Sequence measurement

About 500ng plasmid pGEM-T (containing the DNA sections that is produced by RT-PCR accordingly) is added in the test tube that O.2ml 3.2pmol sequencing primer and 8 μ l termination preparatory response mixed solutions (Perkin-Elmer) are housed, and this test tube is placed Perkin-Elmer 9600 PCR instrument.Take turns by 25: 96 ℃ 10 seconds, 50 ℃ of 5 seconds and 60 ℃ 4 minutes, amplified reaction.Purified product on G50 Sepadex spin post then, and with its lyophilize, be resuspended to then in 3 μ l 25mM EDTA (pH8.0) and the 50mg/ml dextran blueness carried dye.Specimen on ABI 377 automatic sequencers, and with Sequencher (the sequential analysis program of Gene CodesCorporation) analytical sequence.

Determining of the nucleotide sequence of 3 ' and 5 ' end

At 37 ℃, (Wis.) the genome vRNA (2.5 μ g) (as above) of processing purifying is 30 minutes, to remove the phosphate group of 5 ' end for Epicenter Technologies, Madison with the 10U tobacco acid pyrophosphatase.The RNA that handled is carried out phenol-chloroform extraction, and use ethanol sedimentation.Subsequently, connect 5 ' and 3 ' of RNA at 37 ℃ with 50U T4 RNA-ligase enzyme (Pharmaci Biotech) and held 1 hour,, use ethanol sedimentation with phenol-chloroform extraction.The RNA that reverse transcription connects, and, in single reacts, carry out PCR with GeneAmp GoldRNA PCR test kit (PE Biosystems) with the just opposition (as follows) of primer.As mentioned above, carry out sequencing reaction (Galarza, J.M. is etc., 1996, J of Virol.70:2360-2368) with the Auele Specific Primer of each RNA sections.

The primer that is used for influenza A/Udron/72 (H3N2) order-checking

With " F " mark forward primer, and be added with justice for anti-genome.Reverse primer is with " R " mark, and bears justice for genome.

BpRT primer 1107 F 5 '-TATGGAAAGAATAAAAGAACTACGGAA-3 ' 27-53 that PB2 (sections 1) primer primers F/R primer sequence is crossed over

(SEQ ID NO:23) PCR primer 1108 R 5 '-TCGTTTTTAAACTATTCAACAT-3 ' 2307-2328

(SEQ?ID?NO：24)1107?F????????5′-TATGGAAAGAATAAAAGAACTACGGAA-3′??????????27-53

(SEQ ID NO:23) sequencing primer 1101 F 5 '-AGCAGGTCAATTATATTCAATATG-3 ' 1-24

(SEQ?ID?NO：25)

This primer sequence and PB2 gene order have following difference: site PB2 gene 1101 F5 A G6 A G7 A T8 G C9 C A11 G T12 G T13 T A14 C T16 A T18 T C20 T A21 A T22 T A24 C G1102 R 5 '-AACAAGGTCGTTTTTAAACTATTC-3 ' 2312-2335

(SEQ?ID?NO：26)1103?F?????????5′-AGAACTCTATTCCAACAAATG-3′?????????????1816-1836

(SEQ?ID?NO：27)1104?R?????????5′-AATCGGATATTTCATTGCCAT-3′?????????????178-198

(SEQ ID NO:28; This primer sequence is C at Nucleotide 195, and PB2

Gene order is T) 1105 F 5 '-AGAACTCTATTCCAACAAATGAGGGATGTAGTT-3 ' 1816-1848

(SEQ ID NO:29; This primer sequence is G at Nucleotide 1846, and PB2

Gene order is C) 1106 R 5 '-AATCGGATATTTCATTGCCATCATCCATTTCAT-3 ' 166-198

(SEQ ID NO:30; This primer sequence is C at Nucleotide 195, and PB2

Gene order is T) 1107 F 5 '-TATGGAAAGAATAAAAGAACTACGGAA-3 ' 27-53

(SEQ?ID?NO：23)1108?R?????????5′-TCGTTTTTAAACTATTCAACAT-3′?????????????2307-2328

(SEQ?ID?NO：24)1109?F?????????5′-AGACGTGGTGTTGGTAATGAA-3′??????????????2214-2234

(SEQ?ID?NO：31)1110?F?????????5′-CGAAGAGTTGACATAAACCCT-3′??????????????454-474

(SEQ?ID?NO：32)1111?R????????????????????????5′-TCATCCCTCAICCCCTCACAT-3′?????????????????1943-1963

(SEQ ID NO:33; This primer sequence is C at Nucleotide 1955; And PB2

Gene order is G) 1112 R 5 '-ATTTTCTGTTATCCTCTTGTCA-3 ' 204-224

(SEQ ID NO:34; Compare with actual PB2 gene order, this primer is at nuclear

Also have an additional Nucleotide (T) 1113 F 5 '-GTGGAGTCCGCTGTTTTGAG-3 ' 2083-2102 behind the thuja acid 220

(SEQ?ID?NO：35)1114?F????????????????????????5′-AGGATGGTGGACATTCTTAGG-3′?????????????????904-924

(SEQ?ID?NO：36)1115?R????????????????????????5′-CCGATCAATGCTAACCACTAC-3′?????????????????1507-1527

(SEQ?ID?NO：37)1116?F????????????????????????5′-AGCAAAAGCAGGTCAATTATATTCA-3′?????????????1-25

(SEQ?ID?NO：38)1117?R????????????????????????5′-AGTAGAAACAAGGTCGTTTTTAAAC-3′?????????????2317-2341

(SEQ ID NO:39) is used for determining the primer 1104 R 5 '-AATCGGATATTTCATTGCCAT-3 ' 178-198 of end sequence

(SEQ?ID?NO：28)1109?F????????????????????????5′-AGACGTGGTGTTGGTAATGAA-3′?????????????????2214-2234

(SEQ?ID?NO：31)1112?R????????????????????????5′-ATTTTCTGTTATCCTCTTGTCA-3′????????????????204-224

(SEQ?ID?NO：34)1113?F????????????????????????5′-GTGGAGTCCGCTGTTTTGAG-3′??????????????????2083-2102

BpRT primer 1215 F 5 '-AGCAAAAGCAGGCAAACCAT-3 ' 1-20 that (SEQ ID NO:35) PB1 (sections 2) primer primers F/R primer sequence is crossed over

(SEQ ID NO:40; This primer sequence is A at Nucleotide 4, and the PB1 base

Because of sequence is G) PCR primer 1216 R 5 '-AGTAGAAACAAGGCATTTTTT-3 ' 2321-2341

(SEQ?ID?NO：41)1215?F???????????????5′-AGCAAAAGCAGGCAAACCAT-3′?????????????????????????1-20

(SEQ ID NO:40) sequencing primer 1201 F 5 '-TCGAGCTGAAGAAGCTATGG-3 ' 1745-1764

(SEQ ID NO:42; This primer sequence is G at Nucleotide 1752, and PB1

Gene order is A, and this primer sequence is A at Nucleotide 1761, and PB1

Gene order is G) 1202 R 5 '-GTTCTGTTGACTGTGTCCAT-3 ' 142-161

(SEQ?ID?NO：43)1203?F????5′-TCGAGCTGAAGAAGCTATGGGAGCAGACCCGT-3′???????????????????????1745-1776

(SEQ?ID?NO：44)

This primer sequence and PB1 gene order have following difference: site PB2 gene 1203 F1752 A G1761 G A1770 A G1776 C T1204 R 5 '-GTTCTGTTGACTGTGTCCATGGTGTATCC-3 ' 133-161

(SEQ?ID?NO：45)1205?F???????5′-AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAA-3′???????????1-35

(SEQ?ID?NO：46)1206?R???????5′-ATTAAAAACAAGGCATTTTTTCATGAAGGAC-3′????????????2341-2311

(SEQ ID NO:47; This primer sequence is A at Nucleotide 2337, and PB1

Gene order is G, and this primer sequence is T at Nucleotide 2340, and PB1

Gene order is G) 1207 F 5 '-AATTTCCAGCATGGTGGAGGCCATGGTG-3 ' 2154-2181

(SEQ?ID?NO：48)1208?F?????????????5′-GGGATCTTTGAAAACTCGTG-3′??????????????????325-344

(SEQ?ID?NO：49)1209?R?????????????5′-CAGCATTGTTTACAGACTC-3′?????????????1936-1954

(SEQ?ID?NO：50)1210?F?????????????5′-ACCAAAGATGCAGAAAGAGG-3′????????????706-725

(SEQ?ID?NO：51)1211?R?????????????5′-CTCATATTGATTCCGACTAA-3′????????????1438-1457

(SEQ?ID?NO：52)1212?R?????????????5′-TGTCCACTTC?CCTTTTTCTG?A-3′?????????172-192

(SEQ?ID?NO：53)1213?F?????????????5′-ATTTTTCCCC?AGTAGTTCAT?AC-3′????????2118-2139

(SEQ?ID?NO：54)1214?F?????????????5′-ACGCTGTTGC?AACTACACAC?TCCTG-3′?????1997-2021

(SEQ?ID?NO：55)1215?F?????????????5′-AGCAAAAGCAGGCAAACCAT-3′????????????1-20

(SEQ?ID?NO：40)1216?R?????????????5′-AGTAGAAACAAGGCATTTTTT-3′???????????2321-2341

(SEQ ID NO:41) is used for determining the primer 1204 R 5 '-GTTCTGTTGACTGTGTCCATGGTGTATCC-3 ' 133-161 of end sequence

(SEQ?ID?NO：45)1212?R?????????????5′-TGTCCACTTC?CCTTTTTCTG?A-3′?????????172-192

BpRT primer 1315 F 5 '-AGCAAAAGCA GGTACTGATT CGAGA-3 ' 1-25 that (SEQ ID NO:55) PA (sections 3) primer primers F/R primer sequence is crossed over

(SEQ ID NO:56) PCR primer 1315 F 5 '-AGCAAAAGCA GGTACTGATT CGAGA-3 ' 1-25

(SEQ?ID?NO：56)1316?R????????????????5′-AGTAGAAACA?AGGTACTTTT?TTGGA-3′???????????2209-2233

(SEQ ID NO:57) sequencing primer 1301 F 5 '-GAACCTGGAA CCTTTGATCT t-3 ' 2053-2073

(SEQ?ID?NO：58)1302?R????????????????5′-ATTCACCACT?GTCCAGGCCA?T-3′???????????????280-300

(SEQ?ID?NO：59)

This primer sequence and PA gene order have following difference: site PA gene 1302 F294 T C297 T C300 G A1303 F 5 '-GAACCTGGAA CCTTTGATCT TGAGGGGCTA-3 ' 2053-2082

(SEQ ID NO:60; This primer sequence is A at Nucleotide 2075, and PA

Gene order is G) 1304 R 5 '-ATTCACCACT GTCCAGGCCA TTGACGCGTC-3 ' 271-300

(SEQ?ID?NO：61)

This primer sequence and PA gene order have following difference: site PA gene 1304 F274 G C275 C G276 G C277 T A294 T C297 T C300 G A1305 F 5 '-AGCAAAAGCA GGTAGTGATA G-3 ' 1-21

(SEQ?ID?NO：62)

This primer sequence and PA gene order have following difference: site PA gene 1302 F15 C G20 T A21 C G1306 R 5 '-AGTAGAAACA AGGTA-3 ' 2219-2233

(SEQ?ID?NO：63)1307?F?????5′-AGCGAAAGCA?GGTAGTGATT?CGAGATGGA-3′???????????????????1-29

(SEQ ID NO:64; This primer sequence is G at Nucleotide 4, and the PA gene

Sequence is A) 1308 R 5 '-AGTAGAAACA AGGTACTTTT TTGGACAG-3 ' 2206-2233

(SEQ?ID?NO：65)1309?R???????????5′-TCGATTTGTT?GGAGTGACTG?A-3′???????????????????1782-1802

(SEQ?ID?NO：66)1310?F???????????5′-GCCGAACTTC?TCCTGCCTTG?A-3′????????????????????684-704

(SEQ?ID?NO：67)1311?F????????????5′-GTATTCAATA?GCCTGTATG-3′?????????????????????1957-1975

(SEQ?ID?NO：68)1312?R????????????5′-GACTTCTCTC?CTTGTCACTC-3′?????????????????????386-405

(SEQ?ID?NO：69)1313?F???????????5′-ACGAGTCAGC?TAAAGTGGGC?A-3′???????????????????1111-1131

(SEQ?ID?NO：70)1314?R???????????5′-GACACCTCTG?CTGTGAAGTA?A-3′???????????????????1356-1376

(SEQ?ID?NO：71)1315?F?????????5′-AGCAAAAGCA?GGTACTGATT?CGAGA-3′????????????????????1-25

(SEQ?ID?NO：56)1316?R?????????5′-AGTAGAAACA?AGGTACTTTT?TTGGA-3′?????????????????2209-2233

(SEQ?ID?NO：57)1317?F???????????5′-GGAGCTGAGA?AACCGAAGTT?T-3′????????????????????319-339

(SEQ?ID?NO：72)1318?R??????????5′-GACTTGGCCA?ATAAAGTCCT?A-3′????????????????????1935-1955

(SEQ?ID?NO：73)1319?R???????????????5′-GCCTGCGCAT?AGTTCCTGTG?A-3′???????????644-664

(SEQ?ID?NO：74)1320?R???????????????5′-TCTACCACTA?TTGACTCGCC-3′?????????????196-215

(SEQ ID NO:75) is used for determining the primer 1301 F 5 '-GAACCTGGAA CCTTTGATCT t-3 ' 2053-2073 of end sequence

(SEQ?ID?NO：58)1303?F???????????5′-GAACCTGGAA?CCTTTGATCT?TGAGGGGCTA-3′??????2053-2082

(SEQ?ID?NO：60)1311?F????????????????5′-GTATTCAATA?GCCTGTATG-3′?????????????1957-1975

(SEQ?ID?NO：68)1312?R???????????????5′-GACTTCTCTC?CTTGTCACTC-3′??????????????386-405

(SEQ?ID?NO：69)1320?R???????????????5′-TCTACCACTA?TTGACTCGCC-3′??????????????196-215

BpRT primer 1401 F 5 '-AGCAAAAGCA GGGGATAATT CTA-3 ' 1-23 that (SEQ ID NO:75) HA (sections 4) primer primers F/R primer sequence is crossed over

(SEQ ID NO:76) PCR primer 1401 F 5 '-AGCAAAAGCA GGGGATAATT CTA-3 ' 1-23

(SEQ?ID?NO：76)1402?R???????????????5′-AGTAGAAACA?AGGGTGTTTT?TAA-3′??????????1743-1765

(SEQ ID NO:77) sequencing primer 1401 F 5 '-AGCAAAAGCA GGGGATAATT CTA-3 ' 1-23

(SEQ?ID?NO：77)1403?R???????????????5′-ACAGTTTGTT?CATTTCCGAG-3′??????????????1400-1419

(SEQ?ID?NO：78)1404?R??????????????????????????5′-ACTTCAGGGT?GTTTTGCTTA-3′??????????????1004-1023

(SEQ?ID?NO：79)1405?R??????????????????????????5′-GAACCCCCCA?AATGTATAGT-3′??????????????605-624

(SEQ?ID?NO：80)1406?R??????????????????????????5′-TGAGGAACTC?TGAACCAGCT-3′??????????????199-218

(SEQ?ID?NO：81)1407?F??????????????????????????5′-GTCACTAGTT?GCCTCGTCAG-3′??????????????404-423

(SEQ?ID?NO：82)1408?F??????????????????????????5′-CCGGGAGACA?TACTGGTAAT-3′??????????????792-811

(SEQ?ID?NO：83)1409?F??????????????????????????5′-CAAATCAATG?GGAAACTGAA-3′??????????????1203-1222

(SEQ?ID?NO：84)1410?F??????????????????????????5′-TGAACTGAAG?TCAGGATACA-3′??????????????1592-1611

(SEQ ID NO:85) is used for determining the primer 1405 R 5 '-GAACCCCCCA AATGTATAGT-3 ' 605-624 of end sequence

(SEQ?ID?NO：81)1409?F??????????????????????????5′-CAAATCAATG?GGAAACTGAA-3′??????????????1203-1222

BpRT primer 1509 F 5 '-AGCAAAAGCA GGGTTAATAA TCAC-3 ' 1-24 that (SEQ ID NO:85) NP (sections 5) primer primers F/R primer sequence is crossed over

(SEQ ID NO:86) PCR primer 1509 F 5 '-AGCAAAAGCA GGGTTAATAA TCAC-3 ' 1-24

(SEQ?ID?NO：86)1510?R???????????????????5′-AGTAGAAACA?AGGGTATTTT?TCCT-3′??????????1542-1565

(SEQ ID NO:87) sequencing primer 1501 R 5 '-TTGCACCTTC CATCATCCTT-3 ' 1380-1399

(SEQ?ID?NO：88)1502?R???????????????????5′-GTCTATTCCC?ACTAAAGAGT-3′???????????????932-951

(SEQ?ID?NO：89)1503?R???????????????????5′-TGCATCAGAG?AGCACATCCT-3′???????????????529-548

(SEQ?ID?NO：90)1504?F???????????????????5′-GAACTCGTCC?TTTATGACAA-3′???????????????364-383

(SEQ?ID?NO：91)1505?F???????????????????5′-AGAGCAATGG?ATCAAGT-3′??????????????????751-770

(SEQ ID NO:92; On Nucleotide 761-763, have three base ATG's

Disappearance) 1506 F 5 '-TACTATGGAA TCAAGTACTC-3 ' 1161-1180

(SEQ?ID?NO：93)1507?R???????????????????5′-ATCAATCATC?TTCCCGAC-3′??????????????????130-147

(SEQ?ID?NO：94)1508?F???????????????????5′-AGTGTCCTTC?CGTGGGCG-3′??????????????????1410-1427

(SEQ?ID?NO：95)1509?F???????????????????5′-AGCAAAAGCA?GGGTTAATAA?TCAC-3′????????????1-24

(SEQ?ID?NO：86)1510?R???????????????????5′-AGTAGAAACA?AGGGTATTTT?TCCT-3′???????????1542-1565

(SEQ ID NO:87) is used for determining the primer 1503 R 5 '-TGCATCAGAG AGCACATCCT-3 ' 529-548 of end sequence

(SEQ?ID?NO：90)1506?F???????????????????5′-TACTATGGAA?TCAAGTACTC-3′????????????????1161-1180

BpRT primer 1601 F 5 '-AGCAAAAGCA GGAGTGAAGA TGA-3 ' 1-23 that (SEQ ID NO:93) NA (sections 6) primer primers F/R primer sequence is crossed over

(SEQ ID NO:96) PCR primer 1601 F 5 '-AGCAAAAGCA GGAGTGAAGA TGA-3 ' 1-23

(SEQ?ID?NO：96)1602?R??????????????????5′-AGTAGAAACA?AGGAGTTTTT?TCTA-3′???????????1443-1466

(SEQ ID NO:97) sequencing primer 1601 F 5 '-AGCAAAAGCA GGAGTGAAGA TGA-3 ' 1-23

(SEQ?ID?NO：97)1603?R??????????????????5’-TCGTTGTTTC?TGGGTGTGTC-3′?????????????????989-1008

(SEQ ID NO:98; This primer sequence is T at Nucleotide 992, and the NA base

Because of sequence is C) 1604 R 5 '-TTATCATACC CAGTGACACA-3 ' 596-615

(SEQ?ID?NO：99)1605?R??????????????????5’-ATTATTATTG?GTTCACACGG-3′?????????????????173-192

(SEQ?ID?NO：100)1606?F??????????????????5’-TTATGTGTCA?TGCGATCCTG-3′?????????????????379-398

(SEQ?ID?NO：101)1607?F??????????????????5’-ATTGTTCATA?TTAGCCCATT-3′?????????????????803-822

(SEQ?ID?NO：102)1608?F??????????????????5’-ATAGGTCAGG?TTATTCTGGT-3′????????????????1224-1243

(SEQ ID NO:103) is used for determining primer 1608 the F 5 '-ATAGGTCAGG TTATTCTGGT-3 ' 1224-1243 of end sequence

(SEQ?ID?NO：103)1605?R??????????????????5’-ATTATTATTG?GTTCACACG-3′??????????????????173-192

BpRT primer * 1706 F 5 '-AGCAAAAGCA GGTAG-3 ' 1-15 that (SEQ ID NO:100) M1 (sections 7) primer primers F/R primer sequence is crossed over

(SEQ ID NO:104) PCR primer 1707 R 5 '-AGTAGAAACA AGGTA-3 ' 1013-1027

(SEQ?ID?NO：105)1706?F???????????????????????5’-AGCAAAAGCA?GGTAG-3′????????????????1-15

(SEQ?ID?NO：104)*1701R???????????????????????5’-TTTACTCCAG?CTCTATGCTG?ACAA-3′??????985-1008

(SEQ ID NO:106) these primers of * will increase M1 and M2 gene sequencing primer 1701 R 5 '-TTTACTCCAG CTCTATGCTG ACAA-3 ' 985-1008

(SEQ?ID?NO：106)1702?R???????????????????????5’-GATCCAGCCA?TTTGCTCCAT-3′???????????590-609

(SEQ?ID?NO：107)1703?R???????????????????????5’-GAGGTGACAG?GATTGGTCTT-3′???????????169-188

(SEQ?ID?NO：108)1704?F???????????????????????5’-CATGGACAGA?GCAGTTAAAC-3′???????????301-320

(SEQ?ID?NO：109)°1705F??????????????????????5’-GCGAGTATCA?TTGGGATCTT-3′???????????801-820

(SEQ ID NO:110) ° is M1 and total primer 1705 the F 5 '-GCGAGTATCA TTGGGATCTT-3 ' 801-820 that is used for determining end sequence of M2 sequence

(SEQ?ID?NO：110)1702?R???????????????????????5’-GATCCAGCCA?TTTGCTCCAT-3′???????????590-609

(SEQ ID NO:108) for sequence M2, primer 1701,1705 and 1706 is enough.BpRT primer * 1801F 5 '-AGCAAAAGCA GGGTGACAAA GACA-3 ' 1-24 that NS1 (sections 8) primer primers F/R primer sequence is crossed over

(SEQ ID NO:111) PCR primer 1801 F 5 '-AGCAAAAGCA GGGTGACAAA GACA-3 ' 1-24

(SEQ?ID?NO：111)1802?R???????????????5’-AGTAGAAACA?AGGGTGTTTT?TTAT-3′?????????????????867-890

(SEQ?ID?NO：112)??*1803R???????????????5’-TTTTTTATCA?TTAAATAAGC?TGAA-3′?????????????????851-874

(SEQ ID NO:113) * these primer amplifications NS1 and NS2 gene.Sequencing primer ° 1801F 5 '-AGCAAAAGCA GGGTGACAAA GACA-3 ' 1-24

(SEQ?ID?NO：112)°1803R??????????????5’-TTTTTTATCA?TTAAATAAGC?TGAA-3′?????????????????851-874

(SEQ?ID?NO：113)1804?R???????????????5’-AATTGCATTT?TTGACATCCT-3′??????????????????????541-560

(SEQ?ID?NO：114)1805?R???????????????5’-ATCTTCTCTA?CTATCTGCTT-3′??????????????????????210-229

(SEQ?ID?NO：115)1806?F???????????????5’-CAAGCAATCA?TGGATAAGAA-3′??????????????????????387-406

(SEQ?ID?NO：116)°1807F??????????????5’-GGCGAGAACA?GCTAGGTCAA-3′??????????????????????692-711

(SEQ ID NO:117) ° is NS1 and total primer 1807 the F 5 '-GGCGAGAACA GCTAGGTCAA-3 ' 692-711 that is used for determining end sequence of NS2 sequence

(SEQ?ID?NO：117)1804?R???????????????5’-AATTGCATTT?TTGACATCCT-3′??????????????????????541-560

(SEQ?ID?NO：114)1806?F?????????????????????????5’-CAAGCAATCA?TGGATAAGAA-3′????????????387-406

(SEQ?ID?NO：116)1805?R?????????????????????????5’-ATCTTCTCTA?CTATCTGCTT-3′????????????210-229

(SEQ?ID?NO：115)

For sequence NS2, primer 1801,1803 and 1807 is enough.

Embodiment 2

The PCR test detects the sections of influenza A/Udorn/72 (H3N2) bacterial strain

Detect the existence of the sections of influenza A/Udorn/72 (H3N2) bacterial strain with the PCR test.Homology design and selection PCR primer according to virus sequence as herein described.Followingly carry out this test: sample is carried out the RNA reverse transcription, the selected cDNA zone of pcr amplification corresponding to specificity nucleotide sequence as herein described.On gel, identify amplification PCR products, and hybridize their specificity of checking with the specificity nucleotide probe.

Embodiment 3

ELISA detects the antigen by the sections generation of influenza A/Udorn/72 (H3N2) bacterial strain

With the antigenic existence of ELISA detection by the sections generation of influenza A/Udorn/72 (H3N2) bacterial strain.Homology design and selection peptide according to virus sequence as herein described.Then these peptides are coupled to KLH, and are used for the immunization rabbit, to produce the monospecific polyclonal antibody.In " catching ELISA ", use the selection of these polyclonal antibodies or the combination of polyclone and monoclonal antibody then, to detect proteic existence that produces by this virus sections.

Sequence table

Sequence table＜110〉American Cyanamid Company＜120〉the genomic nucleotide sequence of influenza A/Udorn/72 (H3N2)＜130〉AM100289PCT＜140〉＜141＜150〉60/213,650＜151〉2000-06-23＜160〉117＜170〉PatentIn Ver.2.1 version＜210〉1＜211〉2341＜212〉DNA＜213〉influenza A virus (Influenza A virus)＜220〉＜221〉CDS＜222〉(28) .. (2304)＜400〉1agcaaaagca ggtcaattat attcaat atg gaa aga ata aaa gaa cta cgg aat 54

Met?Glu?Arg?Ile?Lys?Glu?Leu?Arg?Asn

1???????????????5ctg?atg?tcg?cag?tct?cgc?act?cgc?gag?ata?cta?aca?aaa?acc?aca?gtg???102Leu?Met?Ser?Gln?Ser?Arg?Thr?Arg?Glu?Ile?Leu?Thr?Lys?Thr?Thr?Val?10??????????????????15??????????????????20??????????????????25gac?cat?atg?gcc?ata?att?aag?aag?tac?aca?tca?ggg?aga?cag?gaa?aag???150Asp?His?Met?Ala?Ile?Ile?Lys?Lys?Tyr?Thr?Set?Gly?Arg?Gln?Glu?Lys

30??????????????????35??????????????????40aac?ccg?tca?ctt?agg?atg?aaa?tgg?atg?atg?gca?atg?aaa?tat?cca?att???198Asn?Pro?Ser?Leu?Arg?Met?Lys?Trp?Met?Met?Ala?Met?Lys?Tyr?Pro?Ile

45??????????????????50??????????????????55aca?gct?gac?aag?agg?ata?aca?gaa?atg?gtt?cct?gag?aga?aat?gag?caa???246Thr?Ala?Asp?Lys?Arg?Ile?Thr?Glu?Met?Val?Pro?Glu?Arg?Asn?Glu?Gln

60??????????????????65??????????????????70gga?caa?acc?cta?tgg?agt?aaa?atg?agt?gat?gcc?ggg?tca?gat?cga?gtg???294Gly?Gln?Thr?Leu?Trp?Ser?Lys?Met?Ser?Asp?Ala?Gly?Ser?Asp?Arg?Val

75??????????????????80??????????????????85atg?gta?tca?cct?ttg?gcg?gtg?aca?tgg?tgg?aat?aga?aat?gga?cca?gtg???342Met?Val?Ser?Pro?Leu?Ala?Val?Thr?Trp?Trp?Asn?Arg?Asn?Gly?Pro?Val?90??????????????????95?????????????????100?????????????????105aca?agt?acg?gtt?cat?tat?cca?aaa?gtc?tac?aag?act?tat?ttt?gat?aaa???390Thr?Ser?Thr?Val?His?Tyr?Pro?Lys?Val?Tyr?Lys?Thr?Tyr?Phe?Asp?Lys

110?????????????????115?????????????????120gtc?gaa?agg?tta?aaa?cat?gga?acc?ttt?ggc?cct?gtc?cat?ttt?aga?aac???438Val?Glu?Arg?Leu?Lys?His?Gly?Thr?Phe?Gly?Pro?Val?His?Phe?Arg?Asn

125?????????????????130?????????????????135caa?gtc?aaa?ata?cgc?cga?aga?gtt?gac?ata?aac?cct?ggt?cat?gca?gac???486Gln?Val?Lys?Ile?Arg?Arg?Arg?Val?Asp?Ile?Asn?Pro?Gly?His?Ala?Asp

140?????????????????145?????????????????150ctc?agt?gcc?aag?gag?gca?caa?gat?gta?atc?atg?gaa?gtt?gtt?ttc?ccc???534Leu?Ser?Ala?Lys?Glu?Ala?Gln?Asp?Val?Ile?Met?Glu?Val?Val?Phe?Pro

155?????????????????160?????????????????165aat?gaa?gtg?ggg?gcc?agg?ata?cta?acg?tcg?gaa?tca?caa?tta?aca?ata???582Asn?Glu?Val?Gly?Ala?Arg?Ile?Leu?Thr?Ser?Glu?Ser?Gln?Leu?Thr?Ile170?????????????????175?????????????????180?????????????????185acc?aaa?gag?aaa?aaa?gaa?gaa?ctc?caa?gat?tgc?aaa?att?tct?cct?ttg???630Thr?Lys?Glu?Lys?Lys?Glu?Glu?Leu?Gln?Asp?Cys?Lys?Ile?Ser?Pro?Leu

190?????????????????195?????????????????200atg?gtt?gca?tac?atg?tta?gag?aga?gaa?ctt?gtc?cga?aaa?acg?aga?ttt???678Met?Val?Ala?Tyr?Met?Leu?Glu?Arg?Glu?Leu?Val?Arg?Lys?Thr?Arg?Phe

205?????????????????210?????????????????215ctc?cca?gtt?gct?ggt?gga?aca?agc?agt?gtg?tac?att?gaa?gtg?tta?cac???726Leu?Pro?Val?Ala?Gly?Gly?Thr?Ser?Ser?Val?Tyr?Ile?Glu?Val?Leu?His

220?????????????????225?????????????????230ttg?act?caa?gga?acg?tgt?tgg?gaa?cag?atg?tac?act?cca?ggt?gga?gaa???774Leu?Thr?Gln?Gly?Thr?Cys?Trp?Glu?Gln?Met?Tyr?Thr?Pro?Gly?Gly?Glu

235?????????????????240?????????????????245gtg?agg?aat?gac?gat?gtt?gac?caa?agc?cta?att?att?gca?gcc?agg?aac???822Val?Arg?Asn?Asp?Asp?Val?Asp?Gln?Ser?Leu?Ile?Ile?Ala?Ala?Arg?Asn250?????????????????255?????????????????260?????????????????265ata?gtg?aga?aga?gca?gca?gta?tca?gca?gat?cca?cta?gca?tct?tta?ttg???870Ile?Val?Arg?Arg?Ala?Ala?Val?Ser?Ala?Asp?Pro?Leu?Ala?Ser?Leu?Leu

270?????????????????275?????????????????280gag?atg?tgc?cac?agc?aca?ctg?att?ggc?ggg?aca?agg?atg?gtg?gac?att???918Glu?Met?Cys?His?Ser?Thr?Leu?Ile?Gly?Gly?Thr?Arg?Met?Val?Asp?Ile

285?????????????????290?????????????????295ctt?agg?cag?aac?ccg?acg?gaa?gaa?caa?gct?gtg?gat?ata?tgc?aag?gct???966Leu?Arg?Gln?Asn?Pro?Thr?Glu?Glu?Gln?Ala?Val?Asp?Ile?Cys?Lys?Ala

300?????????????????305?????????????????310gca?atg?gga?ctg?agg?atc?agc?tca?tcc?ttc?agt?ttt?ggt?ggg?ttc?aca???1014Ala?Met?Gly?Leu?Arg?Ile?Ser?Ser?Ser?Phe?Ser?Phe?Gly?Gly?Phe?Thr

315?????????????????320?????????????????325ttt?aag?aga?aca?agc?ggg?tca?tca?atc?aaa?aga?gag?gaa?gaa?gtg?ctt???1062Phe?Lys?Arg?Thr?Ser?Gly?Ser?Ser?Ile?Lys?Arg?Glu?Glu?Glu?Val?Leu330?????????????????335?????????????????340?????????????????345acg?ggc?aat?ctc?caa?aca?ttg?aaa?ata?agg?gtg?cat?gag?ggg?tac?gag???1110Thr?Gly?Asn?Leu?Gln?Thr?Leu?Lys?Ile?Arg?Val?His?Glu?Gly?Tyr?Glu

350?????????????????355?????????????????360gag?ttc?aca?atg?gtg?ggg?aaa?agg?gca?aca?gct?ata?ctc?aga?aaa?gca???1158Glu?Phe?Thr?Met?Val?Gly?Lys?Arg?Ala?Thr?Ala?Ile?Leu?Arg?Lys?Ala

365?????????????????370?????????????????375acc?agg?aga?ttg?gtt?cag?ctt?ata?gtg?agt?gga?agg?gac?gag?cag?tca???1206Thr?Arg?Arg?Leu?Val?Gln?Leu?Ile?Val?Ser?Gly?Arg?Asp?Glu?Gln?Ser

380?????????????????385?????????????????390ata?gcc?gaa?gcg?ata?att?gta?gcc?atg?gtg?ttt?tca?caa?gag?gat?tgc???1254Ile?Ala?Glu?Ala?Ile?Ile?Val?Ala?Met?Val?Phe?Ser?Gln?Glu?Asp?Cys

395?????????????????400?????????????????405atg?ata?aaa?gca?gtt?aga?ggt?gac?ctg?aat?ttc?gtt?aac?agg?gca?aat???1302Met?Ile?Lys?Ala?Val?Arg?Gly?Asp?Leu?Asn?Phe?Val?Asn?Arg?Ala?Asn410?????????????????415?????????????????420?????????????????425cag?cgg?ttg?aat?ccc?atg?cat?caa?ctt?tta?agg?cat?ttt?cag?aaa?gat???1350Gln?Arg?Leu?Asn?Pro?Met?His?Gln?Leu?Leu?Arg?His?Phe?Gln?Lys?Asp

430?????????????????435?????????????????440gcg?aaa?gtg?ctt?ttt?cag?aat?tgg?gga?att?gaa?cat?atc?gac?aat?gtg???1398Ala?Lys?Val?Leu?Phe?Gln?Asn?Trp?Gly?Ile?Glu?His?Ile?Asp?Asn?Val

445?????????????????450?????????????????455atg?gga?atg?gtt?gga?gta?tta?cca?gac?atg?act?cca?agc?aca?gag?atg???1446Met?Gly?Met?Val?Gly?Val?Leu?Pro?Asp?Met?Thr?Pro?Ser?Thr?Glu?Met

460?????????????????465?????????????????470tca?atg?aga?gga?ata?aga?gtc?agc?aaa?atg?ggc?gtg?gat?gaa?tac?tcc???1494Ser?Met?Arg?Gly?Ile?Arg?Val?Ser?Lys?Met?Gly?Val?Asp?Glu?Tyr?Ser

475?????????????????480?????????????????485agc?aca?gag?agg?gta?gtg?gtt?agc?att?gat?cgg?ttt?ttg?aga?gtt?cga???1542Ser?Thr?Glu?Arg?Val?Val?Val?Ser?Ile?Asp?Arg?Phe?Leu?Arg?Val?Arg490?????????????????495?????????????????500?????????????????505gac?caa?cgt?ggg?aat?gta?tta?cta?tct?cct?gag?gag?gtc?agt?gaa?aca???1590Asp?Gln?Arg?Gly?Asn?Val?Leu?Leu?Ser?Pro?Glu?Glu?Val?Ser?Glu?Thr

510?????????????????515?????????????????520cag?ggg?aca?gag?aga?ctg?aca?ata?act?tac?tca?tcg?tca?atg?atg?tgg???1638Gln?Gly?Thr?Glu?Arg?Leu?Thr?Ile?Thr?Tyr?Ser?Ser?Set?Met?Met?Trp

525?????????????????530?????????????????535gag?att?aat?ggc?cct?gag?tca?gtg?ttg?gtc?aat?acc?tat?caa?tgg?atc???1686Glu?Ile?Asn?Gly?Pro?Glu?Ser?Val?Leu?Val?Asn?Thr?Tyr?Gln?Trp?Ile

540?????????????????545?????????????????550atc?aga?aac?tgg?gaa?act?gtt?aaa?att?caa?tgg?tct?cag?aat?cct?aca???1734Ile?Arg?Asn?Trp?Glu?Thr?Val?Lys?Ile?Gln?Trp?Ser?Gln?Asn?Pro?Thr

555?????????????????560?????????????????565atg?ttg?tac?aac?aaa?atg?gaa?ttt?gag?cca?ttt?cag?tct?tta?gtt?cct???1782Met?Leu?Tyr?Asn?Lys?Met?Glu?Phe?Glu?Pro?Phe?Gln?Ser?Leu?Val?Pro570?????????????????575?????????????????580?????????????????585aag?gcc?att?aga?ggc?caa?tac?agt?gga?ttt?gtc?aga?act?cta?ttc?caa???1830Lys?Ala?Ile?Arg?Gly?Gln?Tyr?Ser?Gly?Phe?Val?Arg?Thr?Leu?Phe?Gln

590?????????????????595?????????????????600caa?atg?agg?gat?gta?ctt?ggg?aca?ttt?gat?acc?acc?cag?ata?ata?aaa???1878Gln?Met?Arg?Asp?Val?Leu?Gly?Thr?Phe?Asp?Thr?Thr?Gln?Ile?Ile?Lys

605?????????????????610?????????????????615ctt?ctc?ccc?ttt?gca?gcc?gcc?cca?cca?aag?caa?agt?aga?atg?cag?ttc???1926Leu?Leu?Pro?Phe?Ala?Ala?Ala?Pro?Pro?Lys?Gln?Ser?Arg?Met?Gln?Phe

620?????????????????625?????????????????630tct?tca?ttg?act?gtg?aat?gtg?agg?gga?tca?ggg?atg?aga?ata?ctt?gta???1974Ser?Ser?Leu?Thr?Val?Asn?Val?Arg?Gly?Ser?Gly?Met?Arg?Ile?Leu?Val

635?????????????????640?????????????????645agg?ggc?aat?tct?cct?gta?ttc?aac?tac?aac?aag?acc?act?aaa?aga?cta???2022Arg?Gly?Asn?Ser?Pro?Val?Phe?Asn?Tyr?Asn?Lys?Thr?Thr?Lys?Arg?Leu650?????????????????655?????????????????660?????????????????665aca?att?ctc?gga?aaa?gat?gct?ggc?act?tta?att?gaa?gac?cca?gat?gaa???2070Thr?Ile?Leu?Gly?Lys?Asp?Ala?Gly?Thr?Leu?Ile?Glu?Asp?Pro?Asp?Glu

670?????????????????675?????????????????680agc?aca?tcc?gga?gtg?gag?tcc?gct?gtt?ttg?aga?gga?ttt?ctc?att?cta???2118Ser?Thr?Ser?Gly?Val?Glu?Ser?Ala?Val?Leu?Arg?Gly?Phe?Leu?Ile?Leu

685?????????????????690?????????????????695ggt?aag?gaa?gat?aga?aga?tac?gga?cca?gca?tta?agc?atc?aat?gaa?ctg???2166Gly?Lys?Glu?Asp?Arg?Arg?Tyr?Gly?Pro?Ala?Leu?Ser?Ile?Asn?Glu?Leu

700?????????????????705?????????????????710agt?aac?ctt?gca?aaa?gga?gaa?aag?gct?aat?gtg?cta?att?ggg?caa?gga???2214Ser?Asn?Leu?Ala?Lys?Gly?Glu?Lys?Ala?Asn?Val?Leu?Ile?Gly?Gln?Gly

715?????????????????720?????????????????725gac?gtg?gtg?ttg?gta?atg?aaa?cga?aaa?cgg?gac?tct?agc?ata?ctt?act???2262Asp?Val?Val?Leu?Val?Met?Lys?Arg?Lys?Arg?Asp?Ser?Ser?Ile?Leu?Thr730?????????????????735?????????????????740?????????????????745gac?agc?cag?aca?gcg?acc?aaa?aga?att?cgg?atg?gcc?atc?aat???????????2304Asp?Ser?Gln?Thr?Ala?Thr?Lys?Arg?Ile?Arg?Met?Ala?Ile?Asn

750 755taatgttgaa tagtttaaaa acgaccttgt ttctact 2341＜210〉2＜211〉759＜212〉PRT＜213〉influenza A virus＜400〉2Met Glu Arg Ile Lys Glu Leu Arg Asn Leu Met Ser Gln Sar Arg Thr 15 10 15Arg Glu Ile Leu Thr Lys Thr Thr Val Asp His Met Ala Ile Ile Lys

20??????????????????25??????????????????30Lys?Tyr?Thr?Ser?Gly?Arg?Gln?Glu?Lys?Asn?Pro?Ser?Leu?Arg?Met?Lys

35??????????????????40??????????????????45Trp?Met?Met?Ala?Met?Lys?Tyr?Pro?Ile?Thr?Ala?Asp?Lys?Arg?Ile?Thr

50??????????????????55??????????????????60Glu?Met?Val?Pro?Glu?Arg?Asn?Glu?Gln?Gly?Gln?Thr?Leu?Trp?Ser?Lys?65??????????????????70??????????????????75??????????????????80Met?Ser?Asp?Ala?Gly?Ser?Asp?Arg?Val?Met?Val?Ser?Pro?Leu?Ala?Val

85??????????????????90??????????????????95Thr?Trp?Trp?Asn?Arg?Asn?Gly?Pro?Val?Thr?Ser?Thr?Val?His?Tyr?Pro

100?????????????????105?????????????????110Lys?Val?Tyr?Lys?Thr?Tyr?Phe?Asp?Lys?Val?Glu?Arg?Leu?Lys?His?Gly

115?????????????????120?????????????????125Thr?Phe?Gly?Pro?Val?His?Phe?Arg?Asn?Gln?Val?Lys?Ile?Arg?Arg?Arg

130?????????????????135?????????????????140Val?Asp?Ile?Asn?Pro?Gly?His?Ala?Asp?Leu?Ser?Ala?Lys?Glu?Ala?Gln145?????????????????150?????????????????155?????????????????160Asp?Val?Ile?Met?Glu?Val?Val?Phe?Pro?Asn?Glu?Val?Gly?Ala?Arg?Ile

165?????????????????170?????????????????175Leu?Thr?Ser?Glu?Ser?Gln?Leu?Thr?Ile?Thr?Lys?Glu?Lys?Lys?Glu?Glu

180?????????????????185?????????????????190Leu?Gln?Asp?Cys?Lys?Ile?Ser?Pro?Leu?Met?Val?Ala?Tyr?Met?Leu?Glu

195?????????????????200?????????????????205Arg?Glu?Leu?Val?Arg?Lys?Thr?Arg?Phe?Leu?Pro?Val?Ala?Gly?Gly?Thr

210?????????????????215?????????????????220Ser?Ser?Val?Tyr?Ile?Glu?Val?Leu?His?Leu?Thr?Gln?Gly?Thr?Cys?Trp225?????????????????230?????????????????235?????????????????240Glu?Gln?Met?Tyr?Thr?Pro?Gly?Gly?Glu?Val?Arg?Asn?Asp?Asp?Val?Asp

245?????????????????250?????????????????255Gln?Ser?Leu?Ile?Ile?Ala?Ala?Arg?Asn?Ile?Val?Arg?Arg?Ala?Ala?Val

260?????????????????265?????????????????270Ser?Ala?Asp?Pro?Leu?Ala?Ser?Leu?Leu?Glu?Met?Cys?His?Ser?Thr?Leu

275?????????????????280?????????????????285Ile?Gly?Gly?Thr?Arg?Met?Val?Asp?Ile?Leu?Arg?Gln?Asn?Pro?Thr?Glu

290?????????????????295?????????????????300Glu?Gln?Ala?Val?Asp?Ile?Cys?Lys?Ala?Ala?Met?Gly?Leu?Arg?Ile?Ser305?????????????????310?????????????????315?????????????????320Ser?Ser?Phe?Ser?Phe?Gly?Gly?Phe?Thr?Phe?Lys?Arg?Thr?Ser?Gly?Ser

325?????????????????330?????????????????335Ser?Ile?Lys?Arg?Glu?Glu?Glu?Val?Leu?Thr?Gly?Asn?Leu?Gln?Thr?Leu

340?????????????????345?????????????????350Lys?Ile?Arg?Val?His?Glu?Gly?Tyr?Glu?Glu?Phe?Thr?Met?Val?Gly?Lys

355?????????????????360?????????????????365Arg?Ala?Thr?Ala?Ile?Leu?Arg?Lys?Ala?Thr?Arg?Arg?Leu?Val?Gln?Leu

370?????????????????375?????????????????380Ile?Val?Ser?Gly?Arg?Asp?Glu?Gln?Ser?Ile?Ala?Glu?Ala?Ile?Ile?Val385?????????????????390?????????????????395?????????????????400Ala?Met?Val?Phe?Ser?Gln?Glu?Asp?Cys?Met?Ile?Lys?Ala?Val?Arg?Gly

405?????????????????410?????????????????415Asp?Leu?Asn?Phe?Val?Asn?Arg?Ala?Asn?Gln?Arg?Leu?Asn?Pro?Met?His

420?????????????????425?????????????????430Gln?Leu?Leu?Arg?His?Phe?Gln?Lys?Asp?Ala?Lys?Val?Leu?Phe?Gln?Asn

435?????????????????440?????????????????445Trp?Gly?Ile?Glu?His?Ile?Asp?Asn?Val?Met?Gly?Met?Val?Gly?Val?Leu

450?????????????????455?????????????????460Pro?Asp?Met?Thr?Pro?Ser?Thr?Glu?Met?Ser?Met?Arg?Gly?Ile?Arg?Val465?????????????????470?????????????????475?????????????????480Ser?Lys?Met?Gly?Val?Asp?Glu?Tyr?Ser?Ser?Thr?Glu?Arg?Val?Val?Val

485?????????????????490?????????????????495Ser?Ile?Asp?Arg?Phe?Leu?Arg?Val?Arg?Asp?Gln?Arg?Gly?Asn?Val?Leu

500?????????????????505?????????????????510Leu?Ser?Pro?Glu?Glu?Val?Ser?Glu?Thr?Gln?Gly?Thr?Glu?Arg?Leu?Thr

515?????????????????520?????????????????525Ile?Thr?Tyr?Ser?Ser?Ser?Met?Met?Trp?Glu?Ile?Asn?Gly?Pro?Glu?Ser

530?????????????????535?????????????????540Val?Leu?Val?Asn?Thr?Tyr?Gln?Trp?Ile?Ile?Arg?Asn?Trp?Glu?Thr?Val545?????????????????550?????????????????555?????????????????560Lys?Ile?Gln?Trp?Ser?Gln?Asn?Pro?Thr?Met?Leu?Tyr?Asn?Lys?Met?Glu

565?????????????????570?????????????????575Phe?Glu?Pro?Phe?Gln?Ser?Leu?Val?Pro?Lys?Ala?Ile?Arg?Gly?Gln?Tyr

580?????????????????585?????????????????590Ser?Gly?Phe?Val?Arg?Thr?Leu?Phe?Gln?Gln?Met?Arg?Asp?Val?Leu?Gly

595?????????????????600?????????????????605Thr?Phe?Asp?Thr?Thr?Gln?Ile?Ile?Lys?Leu?Leu?Pro?Phe?Ala?Ala?Ala

610?????????????????615?????????????????620Pro?Pro?Lys?Gln?Ser?Arg?Met?Gln?Phe?Ser?Ser?Leu?Thr?Val?Asn?Val625?????????????????630?????????????????635?????????????????640Arg?Gly?Ser?Gly?Met?Arg?Ile?Leu?Val?Arg?Gly?Asn?Ser?Pro?Val?Phe

645?????????????????650?????????????????655Asn?Tyr?Asn?Lys?Thr?Thr?Lys?Arg?Leu?Thr?Ile?Leu?Gly?Lys?Asp?Ala

660?????????????????665?????????????????670Gly?Thr?Leu?Ile?Glu?Asp?Pro?Asp?Glu?Ser?Thr?Ser?Gly?Val?Glu?Ser

675?????????????????680?????????????????685Ala?Val?Leu?Arg?Gly?Phe?Leu?Ile?Leu?Gly?Lys?Glu?Asp?Arg?Arg?Tyr

690?????????????????695?????????????????700Gly?Pro?Ala?Leu?Ser?Ile?Asn?Glu?Leu?Ser?Asn?Leu?Ala?Lys?Gly?Glu705?????????????????710?????????????????715?????????????????720Lys?Ala?Asn?Val?Leu?Ile?Gly?Gln?Gly?Asp?Val?Val?Leu?Val?Met?Lys

725?????????????????730?????????????????735Arg?Lys?Arg?Asp?Ser?Ser?Ile?Leu?Thr?Asp?Ser?Gln?Thr?Ala?Thr?Lys

740?????????????????745?????????????????750Arg?Ile?Arg?Met?Ala?Ile?Asn

755＜210〉3＜211〉2341＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(25) .. (2295)＜400〉3agcgaaagca ggcaaaccat ttga atg gat gtc aac ccg act tta ctt ttc 51

Met?Asp?Val?Asn?Pro?Thr?Leu?Leu?Phe

1???????????????5ttg?aaa?gtt?cca?gcg?caa?aat?gcc?ata?agc?acc?aca?ttc?cct?tat?act???99Leu?Lys?Val?Pro?Ala?Gln?Asn?Ala?Ile?Ser?Thr?Thr?Phe?Pro?Tyr?Thr?10??????????????????15??????????????????20??????????????????25gga?gat?cct?cca?tac?agc?cat?gga?aca?gga?aca?gga?tac?acc?atg?gac???147Gly?Asp?Pro?Pro?Tyr?Ser?His?Gly?Thr?Gly?Thr?Gly?Tyr?Thr?Met?Asp

30??????????????????35??????????????????40aca?gtc?aac?aga?aca?cat?caa?tat?tca?gaa?aaa?ggg?aag?tgg?aca?aca???195Thr?Val?Asn?Arg?Thr?His?Gln?Tyr?Ser?Glu?Lys?Gly?Lys?Trp?Thr?Thr

45??????????????????50??????????????????55aac?aca?gaa?act?ggg?gcg?ccc?caa?ctt?aac?cca?att?gat?gga?cca?cta???243Asn?Thr?Glu?Thr?Gly?Ala?Pro?Gln?Leu?Asn?Pro?Ile?Asp?Gly?Pro?Leu

60??????????????????65??????????????????70cct?gag?gat?aat?gag?cca?agt?gga?tat?gca?caa?aca?gac?tgt?gtc?ctg???291Pro?Glu?Asp?Asn?Glu?Pro?Ser?Gly?Tyr?Ala?Gln?Thr?Asp?Cys?Val?Leu

75??????????????????80??????????????????85gaa?gca?atg?gct?ttc?ctt?gaa?gaa?tcc?cac?cca?ggg?atc?ttt?gaa?aac???339Glu?Ala?Met?Ala?Phe?Leu?Glu?Glu?Ser?His?Pro?Gly?Ile?Phe?Glu?Asn?90??????????????????95?????????????????100?????????????????105tcg?tgc?ctt?gaa?acg?atg?gaa?gtc?gtt?caa?caa?aca?agg?gtg?gac?aga???387Ser?Cys?Leu?Glu?Thr?Met?Glu?Val?Val?Gln?Gln?Thr?Arg?Val?Asp?Arg

110?????????????????115?????????????????120ctg?acc?caa?ggt?cgt?cag?acc?tat?gat?tgg?aca?tta?aac?aga?aat?caa???435Leu?Thr?Gln?Gly?Arg?Gln?Thr?Tyr?Asp?Trp?Thr?Leu?Asn?Arg?Asn?Gln

125?????????????????130?????????????????135ccg?gcc?gca?act?gca?tta?gcc?aac?act?ata?gaa?gtc?ttc?aga?tcg?aat???483Pro?Ala?Ala?Thr?Ala?Leu?Ala?Asn?Thr?Ile?Glu?Val?Phe?Arg?Ser?Asn

140?????????????????145?????????????????150ggt?cta?aca?gct?aat?gag?tcg?gga?agg?cta?ata?gat?ttc?ctc?aag?gat???531Gly?Leu?Thr?Ala?Asn?Glu?Ser?Gly?Arg?Leu?Ile?Asp?Phe?Leu?Lys?Asp

155?????????????????160?????????????????165gtg?atg?gaa?tca?atg?gat?aaa?gag?gaa?atg?gag?ata?aca?aca?cac?ttc???579Val?Met?Glu?Ser?Met?Asp?Lys?Glu?Glu?Met?Glu?Ile?Thr?Thr?His?Phe170?????????????????175?????????????????180?????????????????185caa?aga?aaa?aga?aga?gta?aga?gac?aac?atg?acc?aag?aaa?atg?gtc?aca???627Gln?Arg?Lys?Arg?Arg?Val?Arg?Asp?Asn?Met?Thr?Lys?Lys?Met?Val?Thr

190?????????????????195?????????????????200caa?aga?aca?ata?gga?aag?aag?aag?cag?aga?gtg?aac?aag?aga?agc?tat???675Gln?Arg?Thr?Ile?Gly?Lys?Lys?Lys?Gln?Arg?Val?Asn?Lys?Arg?Ser?Tyr

205?????????????????210?????????????????215cta?ata?aga?gca?tta?aca?ttg?aac?aca?atg?acc?aaa?gat?gca?gaa?aga???723Leu?Ile?Arg?Ala?Leu?Thr?Leu?Asn?Thr?Met?Thr?Lys?Asp?Ala?Glu?Arg

220?????????????????225?????????????????230ggt?aaa?tta?aag?aga?aga?gct?att?gca?aca?ccc?ggg?atg?caa?atc?aga???771Gly?Lys?Leu?Lys?Arg?Arg?Ala?Ile?Ala?Thr?Pro?Gly?Met?Gln?Ile?Arg

235?????????????????240?????????????????245ggg?ttc?gtg?tac?ttt?gtt?gaa?act?cta?gct?agg?agc?att?tgt?gag?aag???819Gly?Phe?Val?Tyr?Phe?Val?Glu?Thr?Leu?Ala?Arg?Ser?Ile?Cys?Glu?Lys250?????????????????255?????????????????260?????????????????265ctt?gaa?cag?tct?gga?ctt?cca?gtt?gga?ggt?aat?gaa?aag?aag?gcc?aaa???867Leu?Glu?Gln?Ser?Gly?Leu?Pro?Val?Gly?Gly?Asn?Glu?Lys?Lys?Ala?Lys

270?????????????????275?????????????????280ctg?gca?aat?gtt?gtg?aga?aag?atg?atg?act?aat?tca?caa?gac?aca?gag???915Leu?Ala?Asn?Val?Val?Arg?Lys?Met?Met?Thr?Asn?Ser?Gln?Asp?Thr?Glu

285?????????????????290?????????????????295ctt?tct?ttc?aca?att?act?gga?gac?aat?act?aag?tgg?aat?gaa?aat?caa???963Leu?Ser?Phe?Thr?Ile?Thr?Gly?Asp?Asn?Thr?Lys?Trp?Asn?Glu?Asn?Gln

300?????????????????305?????????????????310aat?cct?cga?atg?ttc?ctg?gcg?atg?att?aca?tat?atc?aca?aaa?aat?caa???1011Asn?Pro?Arg?Met?Phe?Leu?Ala?Met?Ile?Thr?Tyr?Ile?Thr?Lys?Asn?Gln

315?????????????????320?????????????????325cct?gaa?tgg?ttc?aga?aac?att?ctg?agc?atc?gca?ccc?ata?atg?ttc?tca???1059Pro?Glu?Trp?Phe?Arg?Asn?Ile?Leu?Ser?Ile?Ala?Pro?Ile?Met?Phe?Ser330?????????????????335?????????????????340?????????????????345aac?aaa?atg?gcg?aga?cta?ggg?aaa?gga?tac?atg?ttc?gaa?agt?aag?aga???1107Asn?Lys?Met?Ala?Arg?Leu?Gly?Lys?Gly?Tyr?Met?Phe?Glu?Ser?Lys?Arg

350?????????????????355?????????????????360atg?aag?ctc?cga?aca?caa?ata?cca?gca?gaa?atg?cta?gca?agc?att?gac???1155Met?Lys?Leu?Arg?Thr?Gln?Ile?Pro?Ala?Glu?Met?Leu?Ala?Ser?Ile?Asp

365?????????????????370?????????????????375cta?aag?tat?ttc?aat?gaa?tca?aca?aga?aag?aaa?att?gag?aaa?ata?aag???1203Leu?Lys?Tyr?Phe?Asn?Glu?Ser?Thr?Arg?Lys?Lys?Ile?Glu?Lys?Ile?Lys

380?????????????????385?????????????????390cct?ctt?cta?ata?gat?ggc?aca?gcg?tca?ttg?agt?cct?gga?atg?atg?atg???1251Pro?Leu?Leu?Ile?Asp?Gly?Thr?Ala?Ser?Leu?Ser?Pro?Gly?Met?Met?Met

395?????????????????400?????????????????405ggc?atg?ttc?aac?atg?cta?agt?acg?gtt?tta?gga?gtc?tca?atc?ctg?aat???1299Gly?Met?Phe?Asn?Met?Leu?Ser?Thr?Val?Leu?Gly?Val?Ser?Ile?Leu?Asn410?????????????????415?????????????????420?????????????????425ctt?ggg?caa?aag?aaa?tac?acc?aaa?aca?aca?tac?tgg?tgg?gat?gga?ctc???1347Leu?Gly?Gln?Lys?Lys?Tyr?Thr?Lys?Thr?Thr?Tyr?Trp?Trp?Asp?Gly?Leu

430?????????????????435?????????????????440caa?tcc?tct?gat?gat?ttt?gct?ctc?ata?gtg?aat?gca?cca?aat?cat?gag???1395Gln?Ser?Ser?Asp?Asp?Phe?Ala?Leu?Ile?Val?Asn?Ala?Pro?Asn?His?Glu

445?????????????????450?????????????????455gga?ata?caa?gca?gga?gtg?gat?aga?ttc?tac?agg?acc?tgc?aag?tta?gtc???1443Gly?Ile?Gln?Ala?Gly?Val?Asp?Arg?Phe?Tyr?Arg?Thr?Cys?Lys?Leu?Val

460?????????????????465?????????????????470gga?atc?aat?atg?agc?aag?aag?aag?tcc?tat?ata?aat?agg?aca?gga?aca???1491Gly?Ile?Asn?Met?Ser?Lys?Lys?Lys?Ser?Tyr?Ile?Asn?Arg?Thr?Gly?Thr

475?????????????????480?????????????????485ttt?gaa?ttc?aca?agc?ttt?ttt?tat?cgc?tat?gga?ttt?gta?gcc?aat?ttt???1539Phe?Glu?Phe?Thr?Ser?Phe?Phe?Tyr?Arg?Tyr?Gly?Phe?Val?Ala?Asn?Phe490?????????????????495?????????????????500?????????????????505agc?atg?gag?ctg?ccc?agt?ttt?gga?gtg?tct?ggg?att?aat?gag?tca?gct???1587Ser?Met?Glu?Leu?Pro?Ser?Phe?Gly?Val?Ser?Gly?Ile?Asn?Glu?Ser?Ala

510?????????????????515?????????????????520gat?atg?agc?att?gga?gta?aca?gtg?ata?aag?aac?aac?atg?ata?aac?aat???1635Asp?Met?Ser?Ile?Gly?Val?Thr?Val?Ile?Lys?Asn?Asn?Met?Ile?Asn?Asn

525?????????????????530?????????????????535gac?ctt?gga?cca?gca?aca?gcc?cag?atg?gct?ctt?caa?ctg?ttc?atc?aag???1683Asp?Leu?Gly?Pro?Ala?Thr?Ala?Gln?Met?Ala?Leu?Gln?Leu?Phe?Ile?Lys

540?????????????????545?????????????????550gac?tac?aga?tat?aca?tat?cgg?tgc?cac?aga?gga?gac?aca?caa?att?cag???1731Asp?Tyr?Arg?Tyr?Thr?Tyr?Arg?Cys?His?Arg?Gly?Asp?Thr?Gln?Ile?Gln

555?????????????????560?????????????????565acg?agg?aga?tca?ttc?gag?cta?aag?aag?ctg?tgg?gag?caa?acc?cgc?tca???1779Thr?Arg?Arg?Ser?Phe?Glu?Leu?Lys?Lys?Leu?Trp?Glu?Gln?Thr?Arg?Ser570?????????????????575?????????????????580?????????????????585aag?gca?gga?cta?ttg?gtt?tca?gat?gga?gga?cca?aac?tta?tac?aat?atc???1827Lys?Ala?Gly?Leu?Leu?Val?Ser?Asp?Gly?Gly?Pro?Asn?Leu?Tyr?Asn?Ile

590?????????????????595?????????????????600cgg?aat?ctt?cac?atc?cct?gaa?gtc?tgc?tta?aag?tgg?gag?cta?atg?gat???1875Arg?Asn?Leu?His?Ile?Pro?Glu?Val?Cys?Leu?Lys?Trp?Glu?Leu?Met?Asp

605?????????????????610?????????????????615gag?gac?tat?cag?gga?aga?ctt?tgt?aat?ccc?ctg?aat?cca?ttt?gtc?agc???1923Glu?Asp?Tyr?Gln?Gly?Arg?Leu?Cys?Asn?Pro?Leu?Asn?Pro?Phe?Val?Ser

620?????????????????625?????????????????630cat?aag?gag?att?gag?tct?gta?aac?aat?gct?gtg?gta?atg?cca?gct?cat???1971His?Lys?Glu?Ile?Glu?Ser?Val?Asn?Asn?Ala?Val?Val?Met?Pro?Ala?His

635?????????????????640?????????????????645ggt?cca?gcc?aag?agc?atg?gaa?tat?gac?gct?gtt?gca?act?aca?cac?tcc???2019Gly?Pro?Ala?Lys?Ser?Met?Glu?Tyr?Asp?Ala?Val?Ala?Thr?Thr?His?Ser650?????????????????655?????????????????660?????????????????665tgg?att?ccc?aag?agg?aac?cgc?tct?att?ctc?aac?aca?agc?caa?agg?gga???2067Trp?Ile?Pro?Lys?Arg?Asn?Arg?Ser?Ile?Leu?Asn?Thr?Ser?Gln?Arg?Gly

670?????????????????675?????????????????680att?ctt?gag?gat?gaa?cag?atg?tat?cag?aag?tgc?tgc?aac?ctg?ttc?gag???2115Ile?Leu?Glu?Asp?Glu?Gln?Met?Tyr?Gln?Lys?Cys?Cys?Asn?Leu?Phe?Glu

685?????????????????690?????????????????695aaa?ttt?ttc?ccc?agt?agt?tca?tac?agg?aga?ccg?gtt?gga?att?tcc?agc???2163Lys?Phe?Phe?Pro?Ser?Ser?Ser?Tyr?Arg?Arg?Pro?Val?Gly?Ile?Ser?Ser

700?????????????????705?????????????????710atg?gtg?gag?gcc?atg?gtg?tct?agg?gcc?cgg?att?gat?gcc?aga?att?gac???2211Met?Val?Glu?Ala?Met?Val?Ser?Arg?Ala?Arg?Ile?Asp?Ala?Arg?Ile?Asp

715?????????????????720?????????????????725ttc?gaa?tct?gga?cgg?att?aag?aaa?gaa?gag?ttc?gcc?gag?atc?atg?aag???2259Phe?Glu?Ser?Gly?Arg?Ile?Lys?Lys?Glu?Glu?Phe?Ala?Glu?Ile?Met?Lys730?????????????????735?????????????????740?????????????????745atc?tgt?tcc?acc?att?gaa?gag?ctc?aga?cgg?caa?aaa?taatgaattt????????2305Ile?Cys?Ser?Thr?Ile?Glu?Glu?Leu?Arg?Arg?Gln?Lys

750 755agcttgtcct tcatgaaaaa atgccttgtt tctact 2341＜210〉4＜211〉757＜212〉PRT＜213〉influenza A virus＜400〉4Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn 15 10 15Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His

20??????????????????25??????????????????30Gly?Thr?Gly?Thr?Gly?Tyr?Thr?Met?Asp?Thr?Val?Asn?Arg?Thr?His?Gln

35??????????????????40??????????????????45Tyr?Ser?Glu?Lys?Gly?Lys?Trp?Thr?Thr?Asn?Thr?Glu?Thr?Gly?Ala?Pro

50??????????????????55??????????????????60Gln?Leu?Asn?Pro?Ile?Asp?Gly?Pro?Leu?Pro?Glu?Asp?Asn?Glu?Pro?Ser?65??????????????????70??????????????????75??????????????????80Gly?Tyr?Ala?Gln?Thr?Asp?Cys?Val?Leu?Glu?Ala?Met?Ala?Phe?Leu?Glu

85??????????????????90??????????????????95Glu?Ser?His?Pro?Gly?Ile?Phe?Glu?Asn?Ser?Cys?Leu?Glu?Thr?Met?Glu

100?????????????????105?????????????????110Val?Val?Gln?Gln?Thr?Arg?Val?Asp?Arg?Leu?Thr?Gln?Gly?Arg?Gln?Thr

115?????????????????120?????????????????125Tyr?Asp?Trp?Thr?Leu?Asn?Arg?Asn?Gln?Pro?Ala?Ala?Thr?Ala?Leu?Ala

130?????????????????135?????????????????140Asn?Thr?Ile?Glu?Val?Phe?Arg?Ser?Asn?Gly?Leu?Thr?Ala?Asn?Glu?Ser145?????????????????150?????????????????155?????????????????160Gly?Arg?Leu?Ile?Asp?Phe?Leu?Lys?Asp?Val?Met?Glu?Ser?Met?Asp?Lys

165?????????????????170?????????????????175Glu?Glu?Met?Glu?Ile?Thr?Thr?His?Phe?Gln?Arg?Lys?Arg?Arg?Val?Arg

180?????????????????185?????????????????190Asp?Asn?Met?Thr?Lys?Lys?Met?Val?Thr?Gln?Arg?Thr?Ile?Gly?Lys?Lys

195?????????????????200?????????????????205Lys?Gln?Arg?Val?Asn?Lys?Arg?Ser?Tyr?Leu?Ile?Arg?Ala?Leu?Thr?Leu

210?????????????????215?????????????????220Asn?Thr?Met?Thr?Lys?Asp?Ala?Glu?Arg?Gly?Lys?Leu?Lys?Arg?Arg?Ala225?????????????????230?????????????????235?????????????????240Ile?Ala?Thr?Pro?Gly?Met?Gln?Ile?Arg?Gly?Phe?Val?Tyr?Phe?Val?Glu

245?????????????????250?????????????????255Thr?Leu?Ala?Arg?Ser?Ile?Cys?Glu?Lys?Leu?Glu?Gln?Ser?Gly?Leu?Pro

260?????????????????265?????????????????270Val?Gly?Gly?Asn?Glu?Lys?Lys?Ala?Lys?Leu?Ala?Asn?Val?Val?Arg?Lys

275?????????????????280?????????????????285Met?Met?Thr?Asn?Ser?Gln?Asp?Thr?Glu?Leu?Ser?Phe?Thr?Ile?Thr?Gly

290?????????????????295?????????????????300Asp?Asn?Thr?Lys?Trp?Asn?Glu?Asn?Gln?Asn?Pro?Arg?Met?Phe?Leu?Ala305?????????????????310?????????????????315?????????????????320Met?Ile?Thr?Tyr?Ile?Thr?Lys?Asn?Gln?Pro?Glu?Trp?Phe?Arg?Asn?Ile

325?????????????????330?????????????????335Leu?Ser?Ile?Ala?Pro?Ile?Met?Phe?Ser?Asn?Lys?Met?Ala?Arg?Leu?Gly

340?????????????????345?????????????????350Lys?Gly?Tyr?Met?Phe?Glu?Ser?Lys?Arg?Met?Lys?Leu?Arg?Thr?Gln?Ile

355?????????????????360?????????????????365Pro?Ala?Glu?Met?Leu?Ala?Ser?Ile?Asp?Leu?Lys?Tyr?Phe?Asn?Glu?Ser

370?????????????????375?????????????????380Thr?Arg?Lys?Lys?Ile?Glu?Lys?Ile?Lys?Pro?Leu?Leu?Ile?Asp?Gly?Thr385?????????????????390?????????????????395?????????????????400Ala?Ser?Leu?Ser?Pro?Gly?Met?Met?Met?Gly?Met?Phe?Asn?Met?Leu?Ser

405?????????????????410?????????????????415Thr?Val?Leu?Gly?Val?Ser?Ile?Leu?Asn?Leu?Gly?Gln?Lys?Lys?Tyr?Thr

420?????????????????425?????????????????430Lys?Thr?Thr?Tyr?Trp?Trp?Asp?Gly?Leu?Gln?Ser?Ser?Asp?Asp?Phe?Ala

435?????????????????440?????????????????445Leu?Ile?Val?Asn?Ala?Pro?Asn?His?Glu?Gly?Ile?Gln?Ala?Gly?Val?Asp

450?????????????????455?????????????????460Arg?Phe?Tyr?Arg?Thr?Cys?Lys?Leu?Val?Gly?Ile?Asn?Met?Ser?Lys?Lys465?????????????????470?????????????????475?????????????????480Lys?Ser?Tyr?Ile?Asn?Arg?Thr?Gly?Thr?Phe?Glu?Phe?Thr?Ser?Phe?Phe

485?????????????????490?????????????????495Tyr?Arg?Tyr?Gly?Phe?Val?Ala?Asn?Phe?Ser?Met?Glu?Leu?Pro?Ser?Phe

500?????????????????505?????????????????510Gly?Val?Ser?Gly?Ile?Asn?Glu?Ser?Ala?Asp?Met?Ser?Ile?Gly?Val?Thr

515?????????????????520?????????????????525Val?Ile?Lys?Asn?Asn?Met?Ile?Asn?Asn?Asp?Leu?Gly?Pro?Ala?Thr?Ala

530?????????????????535?????????????????540Gln?Met?Ala?Leu?Gln?Leu?Phe?Ile?Lys?Asp?Tyr?Arg?Tyr?Thr?Tyr?Arg545?????????????????550?????????????????555?????????????????560Cys?His?Arg?Gly?Asp?Thr?Gln?Ile?Gln?Thr?Arg?Arg?Ser?Phe?Glu?Leu

565?????????????????570?????????????????575Lys?Lys?Leu?Trp?Glu?Gln?Thr?Arg?Ser?Lys?Ala?Gly?Leu?Leu?Val?Ser

580?????????????????585?????????????????590Asp?Gly?Gly?Pro?Asn?Leu?Tyr?Asn?Ile?Arg?Asn?Leu?His?Ile?Pro?Glu

595?????????????????600?????????????????605Val?Cys?Leu?Lys?Trp?Glu?Leu?Met?Asp?Glu?Asp?Tyr?Gln?Gly?Arg?Leu

610?????????????????615?????????????????620Cys?Asn?Pro?Leu?Asn?Pro?Phe?Val?Ser?His?Lys?Glu?Ile?Glu?Ser?Val625?????????????????630?????????????????635?????????????????640Asn?Asn?Ala?Val?Val?Met?Pro?Ala?His?Gly?Pro?Ala?Lys?Ser?Met?Glu

645?????????????????650?????????????????655Tyr?Asp?Ala?Val?Ala?Thr?Thr?His?Ser?Trp?Ile?Pro?Lys?Arg?Asn?Arg

660?????????????????665?????????????????670Ser?Ile?Leu?Asn?Thr?Ser?Gln?Arg?Gly?Ile?Leu?Glu?Asp?Glu?Gln?Met

675?????????????????680?????????????????685Tyr?Gln?Lys?Cys?Cys?Asn?Leu?Phe?Glu?Lys?Phe?Phe?Pro?Ser?Ser?Ser

690?????????????????695?????????????????700Tyr?Arg?Arg?Pro?Val?Gly?Ile?Ser?Ser?Met?Val?Glu?Ala?Met?Val?Ser705?????????????????710?????????????????715?????????????????720Arg?Ala?Arg?Ile?Asp?Ala?Arg?Ile?Asp?Phe?Glu?Ser?Gly?Arg?Ile?Lys

725?????????????????730?????????????????735Lys?Glu?Glu?Phe?Ala?Glu?Ile?Met?Lys?Ile?Cys?Ser?Thr?Ile?Glu?Glu

740?????????????????745?????????????????750Leu?Arg?Arg?Gln?Lys

755＜210〉5＜211〉2233＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(25) .. (2172)＜400〉5agcaaaagca ggtactgatt cgag atg gaa gat ttt gtg cga caa tgc ttc 51

Met?Glu?Asp?Phe?Val?Arg?Gln?Cys?Phe

1???????????????5aat?ccg?atg?att?gtc?gag?ctt?gca?gaa?aag?gca?atg?aaa?gag?tat?gga???99Asn?Pro?Met?Ile?Val?Glu?Leu?Ala?Glu?Lys?Ala?Met?Lys?Glu?Tyr?Gly?10??????????????????15??????????????????20??????????????????25gag?gat?ctg?aaa?atc?gaa?aca?aac?aaa?ttt?gca?gca?ata?tgc?act?cac???147Glu?Asp?Leu?Lys?Ile?Glu?Thr?Asn?Lys?Phe?Ala?Ala?Ile?Cys?Thr?His

30??????????????????35??????????????????40ttg?gag?ata?tgt?ttc?atg?tat?tca?gat?ttt?cat?ttc?atc?aat?gaa?caa???195Leu?Glu?Ile?Cys?Phe?Met?Tyr?Ser?Asp?Phe?His?Phe?Ile?Asn?Glu?Gln

45??????????????????50??????????????????55ggc?gag?tca?ata?gtg?gta?gag?ctt?gat?gat?cca?aat?gca?ctg?tta?aag???243Gly?Glu?Ser?Ile?Val?Val?Glu?Leu?Asp?Asp?Pro?Asn?Ala?Leu?Leu?Lys

60??????????????????65??????????????????70cac?aga?ttt?gaa?ata?ata?gag?gga?aga?gac?cgc?aca?atg?gcc?tgg?aca???291His?Arg?Phe?Glu?Ile?Ile?Glu?Gly?Arg?Asp?Arg?Thr?Met?Ala?Trp?Thr

75??????????????????80??????????????????85gta?gta?aac?agt?att?tgc?aac?act?act?gga?gct?gag?aaa?ccg?aag?ttt???339Val?Val?Asn?Ser?Ile?Cys?Asn?Thr?Thr?Gly?Ala?Glu?Lys?Pro?Lys?Phe?90??????????????????95?????????????????100?????????????????105ctg?cca?gat?ttg?tat?gat?tac?aag?gag?aat?aga?ttc?atc?gag?att?gga???387Leu?Pro?Asp?Leu?Tyr?Asp?Tyr?Lys?Glu?Asn?Arg?Phe?Ile?Glu?Ile?Gly

110?????????????????115?????????????????120gtg?aca?agg?aga?gaa?gtc?cac?ata?tac?tac?ctt?gaa?aag?gcc?aat?aaa???435Val?Thr?Arg?Arg?Glu?Val?His?Ile?Tyr?Tyr?Leu?Glu?Lys?Ala?Asn?Lys

125?????????????????130?????????????????135att?aaa?tct?gag?aat?aca?cac?atc?cac?att?ttc?tca?ttc?act?ggg?gag???483Ile?Lys?Ser?Glu?Asn?Thr?His?Ile?His?Ile?Phe?Ser?Phe?Thr?Gly?Glu

140?????????????????145?????????????????150gaa?atg?gcc?aca?aag?gcc?gac?tac?act?ctt?gat?gat?gaa?agc?agg?gct???531Glu?Met?Ala?Thr?Lys?Ala?Asp?Tyr?Thr?Leu?Asp?Asp?Glu?Ser?Arg?Ala

155?????????????????160?????????????????165agg?atc?aaa?acc?agg?cta?ttt?acc?ata?aga?caa?gaa?atg?gcc?aac?aga???579Arg?Ile?Lys?Thr?Arg?Leu?Phe?Thr?Ile?Arg?Gln?Glu?Met?Ala?Asn?Arg170?????????????????175?????????????????180?????????????????185ggc?ctc?tgg?gat?tcc?ttt?cgt?cag?tcc?gaa?aga?ggc?gaa?gaa?aca?att???627Gly?Leu?Trp?Asp?Ser?Phe?Arg?Gln?Ser?Glu?Arg?Gly?Glu?Glu?Thr?Ile

190?????????????????195?????????????????200gaa?gaa?aga?ttt?gaa?atc?aca?gga?act?atg?cgc?agg?ctt?gcc?gac?caa???675Glu?Glu?Arg?Phe?Glu?Ile?Thr?Gly?Thr?Met?Arg?Arg?Leu?Ala?Asp?Gln

205?????????????????210?????????????????215agt?ctc?ccg?ccg?aac?ttc?tcc?tgc?ctt?gag?aat?ttt?aga?gcc?tat?gtg???723Ser?Leu?Pro?Pro?Asn?Phe?Ser?Cys?Leu?Glu?Asn?Phe?Arg?Ala?Tyr?Val

220?????????????????225?????????????????230gat?gga?ttc?gaa?ccg?aac?ggc?tgc?att?gag?ggc?aag?ctt?tct?caa?atg???771Asp?Gly?Phe?Glu?Pro?Asn?Gly?Cys?Ile?Glu?Gly?Lys?Leu?Ser?Gln?Met

235?????????????????240?????????????????245tcc?aaa?gaa?gtg?aat?gca?aga?att?gaa?cct?ttt?ctg?aag?aca?aca?cca???819Ser?Lys?Glu?Val?Asn?Ala?Arg?Ile?Glu?Pro?Phe?Leu?Lys?Thr?Thr?Pro250?????????????????255?????????????????260?????????????????265aga?cca?atc?aaa?ctt?ccg?gat?ggg?cct?cct?tgt?ttt?cag?cgg?tcc?aaa???867Arg?Pro?Ile?Lys?Leu?Pro?Asp?Gly?Pro?Pro?Cys?Phe?Gln?Arg?Ser?Lys

270?????????????????275?????????????????280ttc?ctt?ctg?atg?gat?gct?tta?aaa?tta?agc?att?gaa?gac?cca?agt?cac???915Phe?Leu?Leu?Met?Asp?Ala?Leu?Lys?Leu?Ser?Ile?Glu?Asp?Pro?Ser?His

285?????????????????290?????????????????295gaa?gga?gag?gga?ata?cca?cta?tat?gat?gcg?atc?aag?tgc?atg?aga?aca???963Glu?Gly?Glu?Gly?Ile?Pro?Leu?Tyr?Asp?Ala?Ile?Lys?Cys?Met?Arg?Thr

300?????????????????305?????????????????310ttc?ttt?gga?tgg?aaa?gaa?ccc?tat?atc?gtc?aaa?cca?cac?gaa?aag?gga???1011Phe?Phe?Gly?Trp?Lys?Glu?Pro?Tyr?Ile?Val?Lys?Pro?His?Glu?Lys?Gly

315?????????????????320?????????????????325ata?aat?cca?aat?tat?ctg?ctg?tca?tgg?aag?caa?gta?ctg?gca?gaa?cta???1059Ile?Asn?Pro?Asn?Tyr?Leu?Leu?Set?Trp?Lys?Gln?Val?Leu?Ala?Glu?Leu330?????????????????335?????????????????340?????????????????345cag?gac?att?gaa?aat?gag?gag?aag?att?cca?aga?act?aaa?aac?atg?aag???1107Gln?Asp?Ile?Glu?Asn?Glu?Glu?Lys?Ile?Pro?Arg?Thr?Lys?Asn?Met?Lys

350?????????????????355?????????????????360aaa?acg?agt?cag?cta?aag?tgg?gca?ctt?ggt?gag?aac?atg?gca?cct?gag???1155Lys?Thr?Ser?Gln?Leu?Lys?Trp?Ala?Leu?Gly?Glu?Asn?Met?Ala?Pro?Glu

365?????????????????370?????????????????375aaa?gta?gac?ttt?gac?aac?tgt?aga?gac?ata?agc?gat?ttg?aag?caa?tat???1203Lys?Val?Asp?Phe?Asp?Asn?Cys?Arg?Asp?Ile?Ser?Asp?Leu?Lys?Gln?Tyr

380?????????????????385?????????????????390gat?agt?gac?gaa?cct?gaa?tta?agg?tca?ctt?tca?agc?tgg?atc?cag?aat???1251Asp?Ser?Asp?Glu?Pro?Glu?Leu?Arg?Ser?Leu?Ser?Ser?Trp?Ile?Gln?Asn

395?????????????????400?????????????????405gag?ttc?aac?aag?gca?tgc?gag?ctg?acc?gat?tca?atc?tgg?ata?gag?ctc???1299Glu?Phe?Asn?Lys?Ala?Cys?Glu?Leu?Thr?Asp?Ser?Ile?Trp?Ile?Glu?Leu410?????????????????415?????????????????420?????????????????425gat?gag?att?gga?gaa?gac?gtg?gct?cca?att?gaa?tac?att?gca?agc?atg???1347Asp?Glu?Ile?Gly?Glu?Asp?Val?Ala?Pro?Ile?Glu?Tyr?Ile?Ala?Ser?Met

430?????????????????435?????????????????440agg?agg?aat?tac?ttc?aca?gca?gag?gtg?tcc?cat?tgc?aga?gcc?aca?gaa???1395Arg?Arg?Asn?Tyr?Phe?Thr?Ala?Glu?Val?Ser?His?Cys?Arg?Ala?Thr?Glu

445?????????????????450?????????????????455tac?ata?atg?aag?ggg?gta?tac?att?aat?act?gcc?ttg?ctt?aat?gca?tcc???1443Tyr?Ile?Met?Lys?Gly?Val?Tyr?Ile?Asn?Thr?Ala?Leu?Leu?Asn?Ala?Ser

460?????????????????465?????????????????470tgt?gca?gca?atg?gac?gat?ttc?caa?cta?att?ccc?atg?ata?agc?aag?tgc???1491Cys?Ala?Ala?Met?Asp?Asp?Phe?Gln?Leu?Ile?Pro?Met?Ile?Ser?Lys?Cys

475?????????????????480?????????????????485aga?act?aaa?gag?gga?agg?cga?aaa?acc?aat?tta?tat?gga?ttc?atc?ata???1539Arg?Thr?Lys?Glu?Gly?Arg?Arg?Lys?Thr?Asn?Leu?Tyr?Gly?Phe?Ile?Ile490?????????????????495?????????????????500?????????????????505aaa?gga?aga?tct?cac?tta?agg?aat?gac?acc?gac?gtg?gta?aac?ttt?gtg???1587Lys?Gly?Arg?Ser?His?Leu?Arg?Asn?Asp?Thr?Asp?Val?Val?Asn?Phe?Val

510?????????????????515?????????????????520agc?atg?gag?ttt?tct?ctc?act?gac?ccg?aga?ctt?gag?cca?cat?aaa?tgg???1635Ser?Met?Glu?Phe?Ser?Leu?Thr?Asp?Pro?Arg?Leu?Glu?Pro?His?Lys?Trp

525?????????????????530?????????????????535gag?aaa?tac?tgt?gtc?ctt?gag?ata?gga?gat?atg?cta?cta?aga?agt?gcc???1683Glu?Lys?Tyr?Cys?Val?Leu?Glu?Ile?Gly?Asp?Met?Leu?Leu?Arg?Ser?Ala

540?????????????????545?????????????????550ata?ggc?cag?atg?tca?agg?cct?atg?ttc?ttg?tat?gtg?agg?aca?aat?gga???1731Ile?Gly?Gln?Met?Ser?Arg?Pro?Met?Phe?Leu?Tyr?Val?Arg?Thr?Asn?Gly

555?????????????????560?????????????????565aca?tca?aag?att?aaa?atg?aaa?tgg?gga?atg?gag?atg?aga?cgt?tgc?ctc???1779Thr?Ser?Lys?Ile?Lys?Met?Lys?Trp?Gly?Met?Glu?Met?Arg?Arg?Cys?Leu570?????????????????575?????????????????580?????????????????585ctt?cag?tca?ctc?caa?caa?atc?gag?agc?atg?att?gaa?gcc?gag?tct?tct???1827Leu?Gln?Ser?Leu?Gln?Gln?Ile?Glu?Ser?Met?Ile?Glu?Ala?Glu?Ser?Ser

590?????????????????595?????????????????600gtc?aaa?gag?aaa?gac?atg?acc?aaa?gag?ttt?ttt?gag?aat?aaa?tca?gaa???1875Val?Lys?Glu?Lys?Asp?Met?Thr?Lys?Glu?Phe?Phe?Glu?Asn?Lys?Ser?Glu

605?????????????????610?????????????????615aca?tgg?ccc?att?ggg?gag?tcc?ccc?aag?gga?gtg?gaa?gaa?ggt?tcc?att???1923Thr?Trp?Pro?Ile?Gly?Glu?Ser?Pro?Lys?Gly?Val?Glu?Glu?Gly?Ser?Ile

620?????????????????625?????????????????630gga?aag?gtc?tgt?agg?act?tta?ttg?gcc?aag?tcg?gta?ttc?aat?agc?ctg???1971Gly?Lys?Val?Cys?Arg?Thr?Leu?Leu?Ala?Lys?Ser?Val?Phe?Asn?Ser?Leu

635?????????????????640?????????????????645tat?gca?tcc?cca?caa?ttg?gaa?gga?ttt?tea?gcg?gag?tca?aga?aaa?ctg???2019Tyr?Ala?Ser?Pro?Gln?Leu?Glu?Gly?Phe?Ser?Ala?Glu?Ser?Arg?Lys?Leu650?????????????????655?????????????????660?????????????????665ctt?ctt?gtc?gtt?cag?gct?ctt?agg?gac?aac?ctt?gaa?cct?gga?acc?ttt???2067Leu?Leu?Val?Val?Gln?Ala?Leu?Arg?Asp?Asn?Leu?Glu?Pro?Gly?Thr?Phe

670?????????????????675?????????????????680gat?ctt?ggg?ggg?cta?tat?gaa?gca?att?gag?gag?tgc?ctg?att?aat?gat???2115Asp?Leu?Gly?Gly?Leu?Tyr?Glu?Ala?Ile?Glu?Glu?Cys?Leu?Ile?Asn?Asp

685?????????????????690?????????????????695ccc?tgg?gtt?ttg?ctt?aat?gcg?tct?tgg?ttc?aac?tcc?ttc?cta?aca?cat???2163Pro?Trp?Val?Leu?Leu?Asn?Ala?Ser?Trp?Phe?Asn?Ser?Phe?Leu?Thr?His

700?????????????????705?????????????????710gca?tta?aga?tagttgtggc?aatgctacta?tttgctatcc?atactgtcca???????????2212Ala?Leu?Arg

715aaaaagtacc ttgtttctac t 2233＜210〉6＜211〉716＜212〉PRT＜213〉influenza A virus＜400〉6Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 15 10 15Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr

20??????????????????25??????????????????30Asn?Lys?Phe?Ala?Ala?Ile?Cys?Thr?His?Leu?Glu?Ile?Cys?Phe?Met?Tyr

35??????????????????40??????????????????45Ser?Asp?Phe?His?Phe?Ile?Asn?Glu?Gln?Gly?Glu?Ser?Ile?Val?Val?Glu

50??????????????????55??????????????????60Leu?Asp?Asp?Pro?Asn?Ala?Leu?Leu?Lys?His?Arg?Phe?Glu?Ile?Ile?Glu?65??????????????????70??????????????????75??????????????????80Gly?Arg?Asp?Arg?Thr?Met?Ala?Trp?Thr?Val?Val?Asn?Ser?Ile?Cys?Asn

85??????????????????90??????????????????95Thr?Thr?Gly?Ala?Glu?Lys?Pro?Lys?Phe?Leu?Pro?Asp?Leu?Tyr?Asp?Tyr

100?????????????????105?????????????????110Lys?Glu?Asn?Arg?Phe?Ile?Glu?Ile?Gly?Val?Thr?Arg?Arg?Glu?Val?His

115?????????????????120?????????????????125Ile?Tyr?Tyr?Leu?Glu?Lys?Ala?Asn?Lys?Ile?Lys?Ser?Glu?Asn?Thr?His

130?????????????????135?????????????????140Ile?His?Ile?Phe?Ser?Phe?Thr?Gly?Glu?Glu?Met?Ala?Thr?Lys?Ala?Asp145?????????????????150?????????????????155?????????????????160Tyr?Thr?Leu?Asp?Asp?Glu?Ser?Arg?Ala?Arg?Ile?Lys?Thr?Arg?Leu?Phe

165?????????????????170?????????????????175Thr?Ile?Arg?Gln?Glu?Met?Ala?Asn?Arg?Gly?Leu?Trp?Asp?Ser?Phe?Arg

180?????????????????185?????????????????190Gln?Ser?Glu?Arg?Gly?Glu?Glu?Thr?Ile?Glu?Glu?Arg?Phe?Glu?Ile?Thr

195?????????????????200?????????????????205Gly?Thr?Met?Arg?Arg?Leu?Ala?Asp?Gln?Ser?Leu?Pro?Pro?Asn?Phe?Ser

210?????????????????215?????????????????220Cys?Leu?Glu?Asn?Phe?Arg?Ala?Tyr?Val?Asp?Gly?Phe?Glu?Pro?Asn?Gly225?????????????????230?????????????????235?????????????????240Cys?Ile?Glu?Gly?Lys?Leu?Ser?Gln?Met?Ser?Lys?Glu?Val?Asn?Ala?Arg

245?????????????????250?????????????????255Ile?Glu?Pro?Phe?Leu?Lys?Thr?Thr?Pro?Arg?Pro?Ile?Lys?Leu?Pro?Asp

260?????????????????265?????????????????270Gly?Pro?Pro?Cys?Phe?Gln?Arg?Ser?Lys?Phe?Leu?Leu?Met?Asp?Ala?Leu

275????????????????280?????????????????285Lys?Leu?Ser?Ile?Glu?Asp?Pro?Ser?His?Glu?Gly?Glu?Gly?Ile?Pro?Leu

290?????????????????295?????????????????300Tyr?Asp?Ala?Ile?Lys?Cys?Met?Arg?Thr?Phe?Phe?Gly?Trp?Lys?Glu?Pro305?????????????????310?????????????????315?????????????????320Tyr?Ile?Val?Lys?Pro?His?Glu?Lys?Gly?Ile?Asn?Pro?Asn?Tyr?Leu?Leu

325?????????????????330?????????????????335Ser?Trp?Lys?Gln?Val?Leu?Ala?Glu?Leu?Gln?Asp?Ile?Glu?Asn?Glu?Glu

340?????????????????345?????????????????350Lys?Ile?Pro?Arg?Thr?Lys?Asn?Met?Lys?Lys?Thr?Ser?Gln?Leu?Lys?Trp

355?????????????????360?????????????????365Ala?Leu?Gly?Glu?Asn?Met?Ala?Pro?Glu?Lys?Val?Asp?Phe?Asp?Asn?Cys

370?????????????????375?????????????????380Arg?Asp?Ile?Ser?Asp?Leu?Lys?Gln?Tyr?Asp?Ser?Asp?Glu?Pro?Glu?Leu385?????????????????390?????????????????395?????????????????400Arg?Ser?Leu?Ser?Ser?Trp?Ile?Gln?Asn?Glu?Phe?Asn?Lys?Ala?Cys?Glu

405?????????????????410?????????????????415Leu?Thr?Asp?Ser?Ile?Trp?Ile?Glu?Leu?Asp?Glu?Ile?Gly?Glu?Asp?Val

420?????????????????425?????????????????430Ala?Pro?Ile?Glu?Tyr?Ile?Ala?Ser?Met?Arg?Arg?Asn?Tyr?Phe?Thr?Ala

435?????????????????440?????????????????445Glu?Val?Ser?His?Cys?Arg?Ala?Thr?Glu?Tyr?Ile?Met?Lys?Gly?Val?Tyr

450?????????????????455?????????????????460Ile?Asn?Thr?Ala?Leu?Leu?Asn?Ala?Ser?Cys?Ala?Ala?Met?Asp?Asp?Phe465?????????????????470?????????????????475?????????????????480Gln?Leu?Ile?Pro?Met?Ile?Ser?Lys?Cys?Arg?Thr?Lys?Glu?Gly?Arg?Arg

485?????????????????490?????????????????495Lys?Thr?Asn?Leu?Tyr?Gly?Phe?Ile?Ile?Lys?Gly?Arg?Ser?His?Leu?Arg

500?????????????????505?????????????????510Asn?Asp?Thr?Asp?Val?Val?Asn?Phe?Val?Ser?Met?Glu?Phe?Ser?Leu?Thr

515?????????????????520?????????????????525Asp?Pro?Arg?Leu?Glu?Pro?His?Lys?Trp?Glu?Lys?Tyr?Cys?Val?Leu?Glu

530?????????????????535?????????????????540Ile?Gly?Asp?Met?Leu?Leu?Arg?Ser?Ala?Ile?Gly?Gln?Met?Ser?Arg?Pro545?????????????????550?????????????????555?????????????????560Met?Phe?Leu?Tyr?Val?Arg?Thr?Asn?Gly?Thr?Ser?Lys?Ile?Lys?Met?Lys

565?????????????????570?????????????????575Trp?Gly?Met?Glu?Met?Arg?Arg?Cys?Leu?Leu?Gln?Ser?Leu?Gln?Gln?Ile

580?????????????????585?????????????????590Glu?Ser?Met?Ile?Glu?Ala?Glu?Ser?Ser?Val?Lys?Glu?Lys?Asp?Met?Thr

595?????????????????600?????????????????605Lys?Glu?Phe?Phe?Glu?Asn?Lys?Ser?Glu?Thr?Trp?Pro?Ile?Gly?Glu?Ser

610?????????????????615?????????????????620Pro?Lys?Gly?Val?Glu?Glu?Gly?Ser?Ile?Gly?Lys?Val?Cys?Arg?Thr?Leu625?????????????????630?????????????????635?????????????????640Leu?Ala?Lys?Ser?Val?Phe?Asn?Ser?Leu?Tyr?Ala?Ser?Pro?Gln?Leu?Glu

645?????????????????650?????????????????655Gly?Phe?Ser?Ala?Glu?Ser?Arg?Lys?Leu?Leu?Leu?Val?Val?Gln?Ala?Leu

660?????????????????665?????????????????670Arg?Asp?Asn?Leu?Glu?Pro?Gly?Thr?Phe?Asp?Leu?Gly?Gly?Leu?Tyr?Glu

675?????????????????680?????????????????685Ala?Ile?Glu?Glu?Cys?Leu?Ile?Asn?Asp?Pro?Trp?Val?Leu?Leu?Asn?Ala

690 695 700Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg705 710 715＜210〉7＜211〉1765＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(30) .. (1727)＜400〉7agcaaaagca ggggataatt ctattaacc atg aag act atc att gct ttg agc 53

Met?Lys?Thr?Ile?Ile?Ala?Leu?Ser

1???????????????5tac?att?ttc?tgt?ctg?gtt?ctc?ggc?caa?aac?ttt?cca?gga?aat?gac?aac???101Tyr?Ile?Phe?Cys?Leu?Val?Leu?Gly?Gln?Asn?Phe?Pro?Gly?Asn?Asp?Asn

10??????????????????15??????????????????20agc?aca?gca?acg?ctg?tgc?ctg?gga?cat?cat?gcg?gtg?cca?aac?gga?aca???149Ser?Thr?Ala?Thr?Leu?Cys?Leu?Gly?His?His?Ala?Val?Pro?Asn?Gly?Thr?25??????????????????30??????????????????35??????????????????40cta?gtg?aaa?aca?atc?aca?aat?gat?cag?att?gaa?gtg?act?aat?gct?act???197Leu?Val?Lys?Thr?Ile?Thr?Asn?Asp?Gln?Ile?Glu?Val?Thr?Asn?Ala?Thr

45??????????????????50??????????????????55gag?ctg?gtt?cag?agt?tcc?tca?acg?ggg?aaa?ata?tgc?aac?aat?cct?cat???245Glu?Leu?Val?Gln?Ser?Ser?Ser?Thr?Gly?Lys?Ile?Cys?Asn?Asn?Pro?His

60??????????????????65??????????????????70cga?atc?ctt?gat?gga?ata?gac?tgc?aca?ctg?ata?gat?gct?cta?ttg?ggg???293Arg?Ile?Leu?Asp?Gly?Ile?Asp?Cys?Thr?Leu?Ile?Asp?Ala?Leu?Leu?Gly

75??????????????????80??????????????????85gac?cct?cat?tgt?gat?ggc?ttt?caa?aat?gag?aca?tgg?gac?ctt?ttc?gtt???341Asp?Pro?His?Cys?Asp?Gly?Phe?Gln?Asn?Glu?Thr?Trp?Asp?Leu?Phe?Val

90??????????????????95?????????????????100gaa?cgc?agc?aaa?gct?ttc?agc?aac?tgt?tac?cct?tat?gat?gtg?cca?gat???389Glu?Arg?Ser?Lys?Ala?Phe?Ser?Asn?Cys?Tyr?Pro?Tyr?Asp?Val?Pro?Asp105?????????????????110?????????????????115?????????????????120tat?gcc?tcc?ctt?agg?tca?cta?gtt?gcc?tcg?tca?ggc?act?ctg?gag?ttt???437Tyr?Ala?Ser?Leu?Arg?Ser?Leu?Val?Ala?Ser?Ser?Gly?Thr?Leu?Glu?Phe

125?????????????????130?????????????????135atc?agt?gaa?ggc?ttc?act?tgg?act?ggg?gtc?act?cag?aat?ggg?gga?agc???485Ile?Ser?Glu?Gly?Phe?Thr?Trp?Thr?Gly?Val?Thr?Gln?Asn?Gly?Gly?Ser

140?????????????????145?????????????????150aat?gct?tgc?aaa?agg?gga?cct?gat?agc?ggt?ttt?ttc?agt?aga?ctg?aac???533Asn?Ala?Cys?Lys?Arg?Gly?Pro?Asp?Ser?Gly?Phe?Phe?Ser?Arg?Leu?Asn

155?????????????????160?????????????????165tgg?ttg?tac?aaa?tca?gga?agc?aca?tat?cca?gtg?ctg?aac?gtg?act?atg???581Trp?Leu?Tyr?Lys?Ser?Gly?Ser?Thr?Tyr?Pro?Val?Leu?Asn?Val?Thr?Met

170?????????????????175?????????????????180cca?aac?aat?gac?aat?ttt?gac?aaa?cta?tac?att?tgg?ggg?gtt?cac?cac???629Pro?Asn?Asn?Asp?Asn?Phe?Asp?Lys?Leu?Tyr?Ile?Trp?Gly?Val?His?His185?????????????????190?????????????????195?????????????????200ccg?agc?acg?gac?caa?gaa?caa?acc?agc?cta?tat?gtt?caa?gca?tca?ggg???677Pro?Ser?Thr?Asp?Gln?Glu?Gln?Thr?Ser?Leu?Tyr?Val?Gln?Ala?Ser?Gly

205?????????????????210?????????????????215aga?gtc?aca?gtc?tct?acc?aag?aga?agc?cag?caa?act?ata?atc?ccg?aat???725Arg?Val?Thr?Val?Ser?Thr?Lys?Arg?Ser?Gln?Gln?Thr?Ile?Ile?Pro?Asn

220?????????????????225?????????????????230atc?ggg?tct?aga?ccc?tgg?gta?agg?ggt?ctg?tct?agt?aga?ata?agc?atc???773Ile?Gly?Ser?Arg?Pro?Trp?Val?Arg?Gly?Leu?Ser?Ser?Arg?Ile?Ser?Ile

235?????????????????240?????????????????245tat?tgg?aca?ata?gtt?aaa?ccg?gga?gac?ata?ctg?gta?att?aat?agt?aat???821Tyr?Trp?Thr?Ile?Val?Lys?Pro?Gly?Asp?Ile?Leu?Val?Ile?Asn?Ser?Asn

250?????????????????255?????????????????260ggg?aac?cta?att?gct?cct?cgg?ggt?tat?ttt?aaa?atg?cgc?act?ggg?aaa???869Gly?Asn?Leu?Ile?Ala?Pro?Arg?Gly?Tyr?Phe?Lys?Met?Arg?Thr?Gly?Lys265?????????????????270?????????????????275?????????????????280agc?tca?ata?atg?agg?tca?gat?gca?cct?att?ggc?acc?tgc?att?tct?gaa???917Ser?Ser?Ile?Met?Arg?Ser?Asp?Ala?Pro?Ile?Gly?Thr?Cys?Ile?Ser?Glu

285?????????????????290?????????????????295tgc?atc?act?cca?aat?gga?agc?att?ccc?aat?gac?aag?ccc?ttt?caa?aac???965Cys?Ile?Thr?Pro?Asn?Gly?Ser?Ile?Pro?Asn?Asp?Lys?Pro?Phe?Gln?Asn

300?????????????????305?????????????????310gta?aac?aag?atc?aca?tat?ggg?gca?tgt?ccc?aag?tat?gtt?aag?caa?aac???1013Val?Asn?Lys?Ile?Thr?Tyr?Gly?Ala?Cys?Pro?Lys?Tyr?Val?Lys?Gln?Asn

315?????????????????320?????????????????325acc?ctg?aag?ttg?gca?aca?ggg?atg?cgg?aat?gta?cca?gag?aaa?caa?act???1061Thr?Leu?Lys?Leu?Ala?Thr?Gly?Met?Arg?Asn?Val?Pro?Glu?Lys?Gln?Thr

330?????????????????335?????????????????340aga?ggc?cta?ttc?agc?gca?ata?gca?ggt?ttc?ata?gaa?aat?ggg?tgg?gag???1109Arg?Gly?Leu?Phe?Ser?Ala?Ile?Ala?Gly?Phe?Ile?Glu?Asn?Gly?Trp?Glu345?????????????????350?????????????????355?????????????????360gga?atg?ata?gac?ggt?tgg?tac?ggt?ttc?agg?cat?caa?aat?tct?gag?ggc???1157Gly?Met?Ile?Asp?Gly?Trp?Tyr?Gly?Phe?Arg?His?Gln?Asn?Ser?Glu?Gly

365?????????????????370?????????????????375aca?gga?caa?gca?gca?gat?ctt?aaa?agc?act?caa?gca?gcc?atc?gac?caa???1205Thr?Gly?Gln?Ala?Ala?Asp?Leu?Lys?Ser?Thr?Gln?Ala?Ala?Ile?Asp?Gln

380?????????????????385?????????????????390atc?aat?ggg?aaa?ctg?aat?agg?gta?atc?gag?aag?acg?aac?gag?aaa?ttc???1253Ile?Asn?Gly?Lys?Leu?Asn?Arg?Val?Ile?Glu?Lys?Thr?Asn?Glu?Lys?Phe

395?????????????????400?????????????????405cat?caa?atc?gaa?aag?gaa?ttc?tca?gaa?gta?gaa?ggg?aga?att?cag?gac???1301His?Gln?Ile?Glu?Lys?Glu?Phe?Ser?Glu?Val?Glu?Gly?Arg?Ile?Gln?Asp

410?????????????????415?????????????????420ctc?gag?aaa?tac?gtt?gaa?gac?act?aaa?ata?gat?ctc?tgg?tct?tac?aat???1349Leu?Glu?Lys?Tyr?Val?Glu?Asp?Thr?Lys?Ile?Asp?Leu?Trp?Ser?Tyr?Asn425?????????????????430?????????????????435?????????????????440gcg?gag?ctt?ctt?gtc?gct?ctg?gag?aac?caa?cat?aca?att?gat?ctg?act???1397Ala?G1u?Leu?Leu?Val?Ala?Leu?Glu?Asn?Gln?His?Thr?Ile?Asp?Leu?Thr

445?????????????????450?????????????????455gac?tcg?gaa?atg?aac?aaa?ctg?ttt?gaa?aaa?aca?agg?agg?caa?ctg?agg???1445Asp?Ser?Glu?Met?Asn?Lys?Leu?Phe?Glu?Lys?Thr?Arg?Arg?Gln?Leu?Arg

460?????????????????465?????????????????470gaa?aat?gct?gag?gac?atg?ggc?aat?ggt?tgc?ttc?aaa?ata?ttc?cac?aaa???1493Glu?Asn?Ala?Glu?Asp?Met?Gly?Asn?Gly?Cys?Phe?Lys?Ile?Phe?His?Lys

475?????????????????480?????????????????485tgt?gac?aat?gct?tgc?ata?ggg?tca?atc?aga?aat?ggg?act?tat?gac?cat???1541Cys?Asp?Asn?Ala?Cys?Ile?Gly?Ser?Ile?Arg?Asn?Gly?Thr?Tyr?Asp?His

490?????????????????495?????????????????500gat?gta?tac?aga?gac?gaa?gca?tta?aac?aac?cgg?ttt?cag?atc?aaa?ggt???1589Asp?Val?Tyr?Arg?Asp?Glu?Ala?Leu?Asn?Asn?Arg?Phe?Gln?Ile?Lys?Gly505?????????????????510?????????????????515?????????????????520gtt?gaa?ctg?aag?tca?gga?tac?aaa?aac?tgg?atc?ctg?tgg?att?tcc?ttt???1637Val?Glu?Leu?Lys?Ser?Gly?Tyr?Lys?Asn?Trp?Ile?Leu?Trp?Ile?Ser?Phe

525?????????????????530?????????????????535gcc?ata?tca?tgc?ttt?ttg?ctt?tgt?gtt?gtt?ttg?ctg?ggg?ttc?atc?atg???1685Ala?Ile?Ser?Cys?Phe?Leu?Leu?Cys?Val?Val?Leu?Leu?Gly?Phe?Ile?Met

540?????????????????545?????????????????550tgg?gcc?tgc?cag?aaa?ggc?aac?att?agg?tgc?aac?att?tgc?att???????????1727Trp?Ala?Cys?Gln?Lys?Gly?Asn?Ile?Arg?Cys?Asn?Ile?Cys?Ile

555 560 565tgagtgtatt agtaattaaa aacacccttg tttctact 1765＜210〉8＜211〉566＜212〉PRT＜213〉influenza A virus＜400〉8Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Leu Gly 15 10 15Gln Asn Phe Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20??????????????????25??????????????????30His?His?Ala?Val?Pro?Asn?Gly?Thr?Leu?Val?Lys?Thr?Ile?Thr?Asn?Asp

35??????????????????40??????????????????45Gln?Ile?Glu?Val?Thr?Asn?Ala?Thr?Glu?Leu?Val?Gln?Ser?Ser?Ser?Thr

50??????????????????55??????????????????60Gly?Lys?Ile?Cys?Asn?Asn?Pro?His?Arg?Ile?Leu?Asp?Gly?Ile?Asp?Cys?65??????????????????70??????????????????75??????????????????80Thr?Leu?Ile?Asp?Ala?Leu?Leu?Gly?Asp?Pro?His?Cys?Asp?Gly?Phe?Gln

85??????????????????90??????????????????95Asn?Glu?Thr?Trp?Asp?Leu?Phe?Val?Glu?Arg?Ser?Lys?Ala?Phe?Ser?Asn

100?????????????????105?????????????????110Cys?Tyr?Pro?Tyr?Asp?Val?Pro?Asp?Tyr?Ala?Ser?Leu?Arg?Ser?Leu?Val

115?????????????????120?????????????????125Ala?Ser?Ser?Gly?Thr?Leu?Glu?Phe?Ile?Ser?Glu?Gly?Phe?Thr?Trp?Thr

130?????????????????135?????????????????140Gly?Val?Thr?Gln?Asn?Gly?Gly?Ser?Asn?Ala?Cys?Lys?Arg?Gly?Pro?Asp145?????????????????150?????????????????155?????????????????160Ser?Gly?Phe?Phe?Ser?Arg?Leu?Asn?Trp?Leu?Tyr?Lys?Ser?Gly?Ser?Thr

165?????????????????170?????????????????175Tyr?Pro?Val?Leu?Asn?Val?Thr?Met?Pro?Asn?Asn?Asp?Asn?Phe?Asp?Lys

180?????????????????185?????????????????190Leu?Tyr?Ile?Trp?Gly?Val?His?His?Pro?Ser?Thr?Asp?Gln?Glu?Gln?Thr

195?????????????????200?????????????????205Ser?Leu?Tyr?Val?Gln?Ala?Ser?Gly?Arg?Val?Thr?Val?Ser?Thr?Lys?Arg

210?????????????????215?????????????????220Ser?Gln?Gln?Thr?Ile?Ile?Pro?Asn?Ile?Gly?Ser?Arg?Pro?Trp?Val?Arg225?????????????????230?????????????????235?????????????????240Gly?Leu?Ser?Ser?Arg?Ile?Ser?Ile?Tyr?Trp?Thr?Ile?Val?Lys?Pro?Gly

245?????????????????250?????????????????255Asp?Ile?Leu?Val?Ile?Asn?Ser?Asn?Gly?Asn?Leu?Ile?Ala?Pro?Arg?Gly

260?????????????????265?????????????????270Tyr?Phe?Lys?Met?Arg?Thr?Gly?Lys?Ser?Ser?Ile?Met?Arg?Ser?Asp?Ala

275?????????????????280?????????????????285Pro?Ile?Gly?Thr?Cys?Ile?Ser?Glu?Cys?Ile?Thr?Pro?Asn?Gly?Ser?Ile

290?????????????????295?????????????????300Pro?Asn?Asp?Lys?Pro?Phe?Gln?Asn?Val?Asn?Lys?Ile?Thr?Tyr?Gly?Ala305?????????????????310?????????????????315?????????????????320Cys?Pro?Lys?Tyr?Val?Lys?Gln?Asn?Thr?Leu?Lys?Leu?Ala?Thr?Gly?Met

325?????????????????330?????????????????335Arg?Asn?Val?Pro?Glu?Lys?Gln?Thr?Arg?Gly?Leu?Phe?Ser?Ala?Ile?Ala

340?????????????????345?????????????????350Gly?Phe?Ile?Glu?Asn?Gly?Trp?Glu?Gly?Met?Ile?Asp?Gly?Trp?Tyr?Gly

355?????????????????360?????????????????365Phe?Arg?His?Gln?Asn?Ser?Glu?Gly?Thr?Gly?Gln?Ala?Ala?Asp?Leu?Lys

370?????????????????375?????????????????380Ser?Thr?Gln?Ala?Ala?Ile?Asp?Gln?Ile?Asn?Gly?Lys?Leu?Asn?Arg?Val385?????????????????390?????????????????395?????????????????400Ile?Glu?Lys?Thr?Asn?Glu?Lys?Phe?His?Gln?Ile?Glu?Lys?Glu?Phe?Ser

405?????????????????410?????????????????415Glu?Val?Glu?Gly?Arg?Ile?Gln?Asp?Leu?Glu?Lys?Tyr?Val?Glu?Asp?Thr

420?????????????????425?????????????????430Lys?Ile?Asp?Leu?Trp?Ser?Tyr?Asn?Ala?Glu?Leu?Leu?Val?Ala?Leu?Glu

435?????????????????440?????????????????445Asn?Gln?His?Thr?Ile?Asp?Leu?Thr?Asp?Ser?Glu?Met?Asn?Lys?Leu?Phe

450?????????????????455?????????????????460Glu?Lys?Thr?Arg?Arg?Gln?Leu?Arg?Glu?Asn?Ala?Glu?Asp?Met?Gly?Asn465?????????????????470?????????????????475?????????????????480Gly?Cys?Phe?Lys?Ile?Phe?His?Lys?Cys?Asp?Asn?Ala?Cys?Ile?Gly?Ser

485?????????????????490?????????????????495Ile?Arg?Asn?Gly?Thr?Tyr?Asp?His?Asp?Val?Tyr?Arg?Asp?Glu?Ala?Leu

500?????????????????505?????????????????510Asn?Asn?Arg?Phe?Gln?Ile?Lys?Gly?Val?Glu?Leu?Lys?Ser?Gly?Tyr?Lys

515?????????????????520?????????????????525Asn?Trp?Ile?Leu?Trp?Ile?Ser?Phe?Ala?Ile?Ser?Cys?Phe?Leu?Leu?Cys

530?????????????????535?????????????????540Val?Val?Leu?Leu?Gly?Phe?Ile?Met?Trp?Ala?Cys?Gln?Lys?Gly?Asn?Ile545?????????????????550?????????????????555?????????????????560Arg?Cys?Asn?Ile?Cys?Ile

565＜210〉9＜211〉1565＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(46) .. (1539)＜400〉9agcaaaagca gggttaataa tcactcactg agtgacatca aaatc atg gcg tcc caa 57

Met?Ala?Ser?Glnggc?acc?aaa?cgg?tct?tat?gaa?cag?atg?gaa?act?gat?ggg?gaa?cgc?cag???105Gly?Thr?Lys?Arg?Ser?Tyr?Glu?Gln?Met?Glu?Thr?Asp?Gly?Glu?Arg?Gln??5??????????????????10??????????????????15??????????????????20aat?gca?act?gag?atc?agg?gca?tcc?gtc?ggg?aag?atg?att?gat?gga?att???153Asn?Ala?Thr?Glu?Ile?Arg?Ala?Ser?Val?Gly?Lys?Met?Ile?Asp?Gly?Ile

25??????????????????30??????????????????35gga?cga?ttc?tac?atc?caa?atg?tgc?act?gaa?ctt?aaa?ctc?agt?gat?tat???201Gly?Arg?Phe?Tyr?Ile?Gln?Met?Cys?Thr?Glu?Leu?Lys?Leu?Ser?Asp?Tyr

40??????????????????45??????????????????50gag?ggg?cga?ttg?atc?cag?aac?agc?tta?aca?ata?gag?aga?atg?gtg?ctc???249Glu?Gly?Arg?Leu?Ile?Gln?Asn?Ser?Leu?Thr?Ile?Glu?Arg?Met?Val?Leu

55??????????????????60??????????????????65tct?gct?ttt?gat?gag?aga?agg?aat?aga?tat?ctg?gaa?gaa?cat?ccc?agc???297Ser?Ala?Phe?Asp?Glu?Arg?Arg?Asn?Arg?Tyr?Leu?Glu?Glu?His?Pro?Ser

70??????????????????75??????????????????80gcg?ggg?aaa?gat?cct?aag?aaa?act?gga?ggg?ccc?ata?tac?aag?aga?gta???345Ala?Gly?Lys?Asp?Pro?Lys?Lys?Thr?Gly?Gly?Pro?Ile?Tyr?Lys?Arg?Val??85?????????????????90??????????????????95?????????????????100gat?aga?aag?tgg?atg?agg?gaa?ctc?gtc?ctt?tat?gac?aaa?gaa?gaa?ata???393Asp?Arg?Lys?Trp?Met?Arg?Glu?Leu?Val?Leu?Tyr?Asp?Lys?Glu?Glu?Ile

105?????????????????110?????????????????115agg?cga?atc?tgg?cgc?caa?gcc?aat?aat?ggt?gat?gat?gcg?aca?gct?ggt???441Arg?Arg?Ile?Trp?Arg?Gln?Ala?Asn?Asn?Gly?Asp?Asp?Ala?Thr?Ala?Gly

120?????????????????125?????????????????130cta?acc?cac?atg?atg?atc?tgg?cat?tcc?aat?ttg?aat?gat?aca?aca?tac???489Leu?Thr?His?Met?Met?Ile?Trp?His?Ser?Asn?Leu?Asn?Asp?Thr?Thr?Tyr

135?????????????????140?????????????????145cag?agg?aca?aga?gct?ctt?gtt?cgc?acc?gga?atg?gat?ccc?agg?atg?tgc???537Gln?Arg?Thr?Arg?Ala?Leu?Val?Arg?Thr?Gly?Met?Asp?Pro?Arg?Met?Cys

150?????????????????155?????????????????160tct?ctg?atg?cag?ggt?tcg?act?ctc?cct?agg?agg?tct?gga?gct?gca?ggc???585Ser?Leu?Met?Gln?Gly?Ser?Thr?Leu?Pro?Arg?Arg?Ser?Gly?Ala?Ala?Gly165?????????????????170?????????????????175?????????????????180gct?gca?gtc?aaa?gga?gtc?ggg?aca?atg?gtg?atg?gag?ctg?atc?aga?atg???633Ala?Ala?Val?Lys?Gly?Val?Gly?Thr?Met?Val?Met?Glu?Leu?Ile?Arg?Met

185?????????????????190?????????????????195atc?aaa?cgt?ggg?atc?aac?gat?cgg?aac?ttc?tgg?aga?ggt?gag?aat?gga???681Ile?Lys?Arg?Gly?Ile?Asn?Asp?Arg?Asn?Phe?Trp?Arg?Gly?Glu?Asn?Gly

200?????????????????205?????????????????210cga?aaa?aca?agg?ggt?gct?tat?gag?aga?atg?tgc?aac?att?ctc?aaa?gga???729Arg?Lys?Thr?Arg?Gly?Ala?Tyr?Glu?Arg?Met?Cys?Asn?Ile?Leu?Lys?Gly

215?????????????????220?????????????????225aaa?ttt?caa?aca?gct?gca?caa?aga?gca?atg?atg?gat?caa?gtg?aga?gaa???777Lys?Phe?Gln?Thr?Ala?Ala?Gln?Arg?Ala?Met?Met?Asp?Gln?Val?Arg?Glu

230?????????????????235?????????????????240agc?cgg?aac?cca?gga?aat?gct?gag?atc?gaa?gat?ctc?ata?ttt?ttg?gca???825Ser?Arg?Asn?Pro?Gly?Asn?Ala?Glu?Ile?Glu?Asp?Leu?Ile?Phe?Leu?Ala245?????????????????250?????????????????255?????????????????260cgg?tct?gca?cta?ata?ttg?aga?ggg?tca?gtt?gct?cac?aaa?tct?tgt?ctg???873Arg?Ser?Ala?Leu?Ile?Leu?Arg?Gly?Ser?Val?Ala?His?Lys?Ser?Cys?Leu

265?????????????????270?????????????????275cct?gcc?tgt?gtg?tat?gga?cct?gcc?gta?gcc?agt?ggg?tac?gac?ttc?gaa???921Pro?Ala?Cys?Val?Tyr?Gly?Pro?Ala?Val?Ala?Ser?Gly?Tyr?Asp?Phe?Glu

280?????????????????285?????????????????290aaa?gag?gga?tac?tct?tta?gtg?gga?ata?gac?cct?ttc?aaa?cta?ctt?caa???969Lys?Glu?Gly?Tyr?Ser?Leu?Val?Gly?Ile?Asp?Pro?Phe?Lys?Leu?Leu?Gln

295?????????????????300?????????????????305aac?agc?caa?gta?tac?agc?cta?atc?aga?ccg?aac?gag?aat?cca?gca?cac???1017Asn?Ser?Gln?Val?Tyr?Ser?Leu?Ile?Arg?Pro?Asn?Glu?Asn?Pro?Ala?His

310?????????????????315?????????????????320aag?agt?cag?ctg?gtg?tgg?atg?gca?tgc?aat?tct?gct?gca?ttt?gaa?gat???1065Lys?Ser?Gln?Leu?Val?Trp?Met?Ala?Cys?Asn?Ser?Ala?Ala?Phe?Glu?Asp325?????????????????330?????????????????335?????????????????340cta?aga?tta?tta?agc?ttc?atc?aga?ggg?acc?aaa?gta?tct?cca?agg?ggg???1113Leu?Arg?Leu?Leu?Ser?Phe?Ile?Arg?Gly?Thr?Lys?Val?Ser?Pro?Arg?Gly

345?????????????????350?????????????????355aaa?ctt?tca?act?aga?gga?gta?caa?att?gct?tca?aat?gaa?aac?atg?gat???1161Lys?Leu?Ser?Thr?Arg?Gly?Val?Gln?Ile?Ala?Ser?Asn?Glu?Asn?Met?Asp

360?????????????????365?????????????????370act?atg?gaa?tca?agt?act?ctt?gaa?ctg?aga?agc?agg?tac?tgg?gcc?ata???1209Thr?Met?Glu?Ser?Ser?Thr?Leu?Glu?Leu?Arg?Ser?Arg?Tyr?Trp?Ala?Ile

375?????????????????380?????????????????385agg?acc?aga?agt?gga?gga?aac?act?aat?caa?cag?agg?gcc?tcc?gca?ggc???1257Arg?Thr?Arg?Ser?Gly?Gly?Asn?Thr?Asn?Gln?Gln?Arg?Ala?Ser?Ala?Gly

390?????????????????395?????????????????400caa?atc?agt?gtg?caa?cct?gca?ttt?tct?gtg?caa?aga?aac?ctc?cca?ttt???1305Gln?Ile?Ser?Val?Gln?Pro?Ala?Phe?Ser?Val?Gln?Arg?Asn?Leu?Pro?Phe405?????????????????410?????????????????415?????????????????420gac?aaa?tca?acc?atc?atg?gca?gca?ttc?act?ggg?aat?acg?gag?gga?aga???1353Asp?Lys?Ser?Thr?Ile?Met?Ala?Ala?Phe?Thr?Gly?Asn?Thr?Glu?Gly?Arg

425?????????????????430?????????????????435acc?tca?gac?atg?agg?gca?gaa?atc?ata?agg?atg?atg?gaa?ggt?gca?aaa???1401Thr?Ser?Asp?Met?Arg?Ala?Glu?Ile?Ile?Arg?Met?Met?Glu?Gly?Ala?Lys

440?????????????????445?????????????????450cca?gaa?gaa?gtg?tcc?ttc?cgt?ggg?cgg?gga?gtc?ttc?gag?ctc?tcg?gac???1449Pro?Glu?Glu?Val?Ser?Phe?Arg?Gly?Arg?Gly?Val?Phe?Glu?Leu?Ser?Asp

455?????????????????460?????????????????465gaa?aag?gca?acg?aac?ccg?atc?gtg?ccc?tct?ttt?gac?atg?agt?aat?gaa???1497Glu?Lys?Ala?Thr?Asn?Pro?Ile?Val?Pro?Ser?Phe?Asp?Met?Ser?Asn?Glu

470 475 480gga tct tat ttc ttc gga gac aat gca gag gag tac gac aat 1539Gly Ser Tyr Phe Phe Gly Asp Asn Ala Glu Glu Tyr Asp Asn485 490 495taaggaaaaa tacccttgtt tctact 1565＜210〉10＜211〉498＜212〉PRT＜213〉influenza A virus＜400〉10Met Ala Ser Gln Gly Thr Lys Arg Ser Tyr Glu Gln Met Glu Thr Asp 15 10 15Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val Gly Lys Met

20??????????????????25??????????????????30Ile?Asp?Gly?Ile?Gly?Arg?Phe?Tyr?Ile?Gln?Met?Cys?Thr?Glu?Leu?Lys

35??????????????????40??????????????????45Leu?Ser?Asp?Tyr?Glu?Gly?Arg?Leu?Ile?Gln?Asn?Ser?Leu?Thr?Ile?Glu

50??????????????????55??????????????????60Arg?Met?Val?Leu?Ser?Ala?Phe?Asp?Glu?Arg?Arg?Asn?Arg?Tyr?Leu?Glu?65??????????????????70??????????????????75??????????????????80Glu?His?Pro?Ser?Ala?Gly?Lys?Asp?Pro?Lys?Lys?Thr?Gly?Gly?Pro?Ile

85??????????????????90??????????????????95Tyr?Lys?Arg?Val?Asp?Arg?Lys?Trp?Met?Arg?Glu?Leu?Val?Leu?Tyr?Asp

100?????????????????105?????????????????110Lys?Glu?Glu?Ile?Arg?Arg?Ile?Trp?Arg?Gln?Ala?Asn?Asn?Gly?Asp?Asp

115?????????????????120?????????????????125Ala?Thr?Ala?Gly?Leu?Thr?His?Met?Met?Ile?Trp?His?Ser?Asn?Leu?Asn

130?????????????????135?????????????????140Asp?Thr?Thr?Tyr?Gln?Arg?Thr?Arg?Ala?Leu?Val?Arg?Thr?Gly?Met?Asp145?????????????????150?????????????????155?????????????????160Pro?Arg?Met?Cys?Ser?Leu?Met?Gln?Gly?Ser?Thr?Leu?Pro?Arg?Arg?Ser

165?????????????????170?????????????????175Gly?Ala?Ala?Gly?Ala?Ala?Val?Lys?Gly?Val?Gly?Thr?Met?Val?Met?Glu

180?????????????????185?????????????????190Leu?Ile?Arg?Met?Ile?Lys?Arg?Gly?Ile?Asn?Asp?Arg?Asn?Phe?Trp?Arg

195?????????????????200?????????????????205Gly?Glu?Asn?Gly?Arg?Lys?Thr?Arg?Gly?Ala?Tyr?Glu?Arg?Met?Cys?Asn

210?????????????????215?????????????????220Ile?Leu?Lys?Gly?Lys?Phe?Gln?Thr?Ala?Ala?Gln?Arg?Ala?Met?Met?Asp225?????????????????230?????????????????235?????????????????240Gln?Val?Arg?Glu?Ser?Arg?Asn?Pro?Gly?Asn?Ala?Glu?Ile?Glu?Asp?Leu

245?????????????????250?????????????????255Ile?Phe?Leu?Ala?Arg?Ser?Ala?Leu?Ile?Leu?Arg?Gly?Ser?Val?Ala?His

260?????????????????265?????????????????270Lys?Ser?Cys?Leu?Pro?Ala?Cys?Val?Tyr?Gly?Pro?Ala?Val?Ala?Ser?Gly

275?????????????????280?????????????????285Tyr?Asp?Phe?Glu?Lys?Glu?Gly?Tyr?Ser?Leu?Val?Gly?Ile?Asp?Pro?Phe

290?????????????????295?????????????????300Lys?Leu?Leu?Gln?Asn?Ser?Gln?Val?Tyr?Ser?Leu?Ile?Arg?Pro?Asn?Glu305?????????????????310?????????????????315?????????????????320Asn?Pro?Ala?His?Lys?Ser?Gln?Leu?Val?Trp?Met?Ala?Cys?Asn?Ser?Ala

325?????????????????330?????????????????335Ala?Phe?Glu?Asp?Leu?Arg?Leu?Leu?Ser?Phe?Ile?Arg?Gly?Thr?Lys?Val

340?????????????????345?????????????????350Ser?Pro?Arg?Gly?Lys?Leu?Ser?Thr?Arg?Gly?Val?Gln?Ile?Ala?Ser?Asn

355?????????????????360?????????????????365Glu?Asn?Met?Asp?Thr?Met?Glu?Ser?Ser?Thr?Leu?Glu?Leu?Arg?Ser?Arg

370?????????????????375?????????????????380Tyr?Trp?Ala?Ile?Arg?Thr?Arg?Ser?Gly?Gly?Asn?Thr?Asn?Gln?Gln?Arg385?????????????????390?????????????????395?????????????????400Ala?Ser?Ala?Gly?Gln?Ile?Ser?Val?Gln?Pro?Ala?Phe?Ser?Val?Gln?Arg

405?????????????????410?????????????????415Asn?Leu?Pro?Phe?Asp?Lys?Ser?Thr?Ile?Met?Ala?Ala?Phe?Thr?Gly?Asn

420?????????????????425?????????????????430Thr?Glu?Gly?Arg?Thr?Ser?Asp?Met?Arg?Ala?Glu?Ile?Ile?Arg?Met?Met

435?????????????????440?????????????????445Glu?Gly?Ala?Lys?Pro?Glu?Glu?Val?Ser?Phe?Arg?Gly?Arg?Gly?Val?Phe

450?????????????????455?????????????????460Glu?Leu?Ser?Asp?Glu?Lys?Ala?Thr?Asn?Pro?Ile?Val?Pro?Ser?Phe?Asp465?????????????????470?????????????????475?????????????????480Met?Ser?Asn?Glu?Gly?Ser?Tyr?Phe?Phe?Gly?Asp?Asn?Ala?Glu?Glu?Tyr

485 490 495Asp Asn＜210〉11＜211〉1466＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(20) .. (1426)＜400〉11agcaaaagca ggagtgaag atg aat cca aat caa aag ata ata aca att ggc 52

Met?Asn?Pro?Asn?Gln?Lys?Ile?Ile?Thr?Ile?Gly

1???????????????5??????????????????10tct?gtc?tct?ctc?acc?att?gca?aca?ata?tgc?ttc?ctc?atg?cag?att?gcc???100Ser?Val?Ser?Leu?Thr?Ile?Ala?Thr?Ile?Cys?Phe?Leu?Met?Gln?Ile?Ala

15??????????????????20??????????????????25atc?ctg?gta?act?act?gta?aca?ttg?cat?ttc?aag?caa?tat?gag?tgc?gac???148Ile?Leu?Val?Thr?Thr?Val?Thr?Leu?His?Phe?Lys?Gln?Tyr?Glu?Cys?Asp

30??????????????????35??????????????????40tcc?ccc?gcg?aac?aac?caa?gta?atg?ccg?tgt?gaa?cca?ata?ata?ata?gaa???196Ser?Pro?Ala?Asn?Asn?Gln?Val?Met?Pro?Cys?Glu?Pro?Ile?Ile?Ile?Glu

45??????????????????50??????????????????55agg?aac?ata?aca?gag?ata?gtg?tat?ttg?act?aac?acc?acc?ata?gag?aaa???244Arg?Asn?Ile?Thr?Glu?Ile?Val?Tyr?Leu?Thr?Asn?Thr?Thr?Ile?Glu?Lys?60??????????????????65??????????????????70??????????????????75gag?ata?tgc?ccc?aaa?tta?gtg?gaa?tac?agg?aat?tgg?tca?aag?ccg?caa???292Glu?Ile?Cys?Pro?Lys?Leu?Val?Glu?Tyr?Arg?Asn?Trp?Ser?Lys?Pro?Gln

80??????????????????85??????????????????90tgt?aaa?att?aca?gga?ttt?gca?cct?ttt?tct?aag?gac?aat?tca?att?cgg???340Cys?Lys?Ile?Thr?Gly?Phe?Ala?Pro?Phe?Ser?Lys?Asp?Asn?Ser?Ile?Arg

95?????????????????100?????????????????105ctt?tct?gct?ggt?ggg?gac?att?tgg?gtg?acg?aga?gaa?cct?tat?gtg?tca???388Leu?Ser?Ala?Gly?Gly?Asp?Ile?Trp?Val?Thr?Arg?Glu?Pro?Tyr?Val?Ser

110?????????????????115?????????????????120tgc?gat?cct?ggc?aag?tgt?tat?caa?ttt?gca?ctc?ggg?cag?ggg?acc?aca???436Cys?Asp?Pro?Gly?Lys?Cys?Tyr?Gln?Phe?Ala?Leu?Gly?Gln?Gly?Thr?Thr

125?????????????????130?????????????????135cta?gac?aac?aaa?cat?tca?aat?gac?aca?ata?cat?gat?aga?acc?cct?cat???484Leu?Asp?Asn?Lys?His?Ser?Asn?Asp?Thr?Ile?His?Asp?Arg?Thr?Pro?His140?????????????????145?????????????????150?????????????????155cga?acc?cta?ttg?atg?aat?gag?ttg?ggt?gtt?cca?ttt?cat?ttg?gga?acc???532Arg?Thr?Leu?Leu?Met?Asn?Glu?Leu?Gly?Val?Pro?Phe?His?Leu?Gly?Thr

160?????????????????165?????????????????170agg?caa?gtg?tgt?ata?gca?tgg?tcc?agc?tca?agt?tgt?cac?gat?gga?aaa???580Arg?Gln?Val?Cys?Ile?Ala?Trp?Ser?Ser?Ser?Ser?Cys?His?Asp?Gly?Lys

175?????????????????180?????????????????185gca?tgg?ctg?cat?gtt?tgt?gtc?act?ggg?tat?gat?aaa?aat?gca?act?gct???628Ala?Trp?Leu?His?Val?Cys?Val?Thr?Gly?Tyr?Asp?Lys?Asn?Ala?Thr?Ala

190?????????????????195?????????????????200agc?ttc?att?tac?gat?ggg?agg?ctt?gta?gac?agt?att?ggt?tca?tgg?tct???676Ser?Phe?Ile?Tyr?Asp?Gly?Arg?Leu?Val?Asp?Ser?Ile?Gly?Ser?Trp?Ser

205?????????????????210?????????????????215caa?aat?atc?ctc?agg?acc?cag?gag?tcg?gaa?tgc?gtt?tgt?atc?aat?ggg???724Gln?Asn?Ile?Leu?Arg?Thr?Gln?Glu?Ser?Glu?Cys?Val?Cys?Ile?Asn?Gly220?????????????????225?????????????????230?????????????????235act?tgt?aca?gta?gta?atg?act?gat?gga?agt?gct?tca?gga?aga?gct?gat???772Thr?Cys?Thr?Val?Val?Met?Thr?Asp?Gly?Ser?Ala?Ser?Gly?Arg?Ala?Asp

240?????????????????245?????????????????250act?aaa?ata?cta?ttc?att?gaa?gag?ggg?aaa?att?gtt?cat?att?agc?cca???820Thr?Lys?Ile?Leu?Phe?Ile?Glu?Glu?Gly?Lys?Ile?Val?His?Ile?Ser?Pro

255?????????????????260?????????????????265ttg?tca?gga?agt?gct?cag?cat?gta?gag?gag?tgt?tcc?tgt?tat?cct?cga???868Leu?Ser?Gly?Ser?Ala?Gln?His?Val?Glu?Glu?Cys?Ser?Cys?Tyr?Pro?Arg

270?????????????????275?????????????????280tat?cct?ggt?gtc?aga?tgt?atc?tgc?aga?gac?aac?tgg?aaa?ggc?tct?aat???916Tyr?Pro?Gly?Val?Arg?Cys?Ile?Cys?Arg?Asp?Asn?Trp?Lys?Gly?Ser?Asn

285?????????????????290?????????????????295agg?ccc?gtc?gta?gat?ata?aat?gtg?aaa?gat?tat?agc?att?gat?tcc?agt???964Arg?Pro?Val?Val?Asp?Ile?Asn?Val?Lys?Asp?Tyr?Ser?Ile?Asp?Ser?Ser300?????????????????305?????????????????310?????????????????315tat?gtg?tgc?tca?ggg?ctt?gtt?ggc?gac?gca?ccc?aga?aac?aac?gac?aga???1012Tyr?Val?Cys?Ser?Gly?Leu?Val?Gly?Asp?Ala?Pro?Arg?Asn?Asn?Asp?Arg

320?????????????????325?????????????????330tct?agc?aat?agc?tat?tgc?cgg?aat?cct?aac?aat?gag?aaa?ggg?aat?cac???1060Ser?Ser?Asn?Ser?Tyr?Cys?Arg?Asn?Pro?Asn?Asn?Glu?Lys?Gly?Asn?His

335?????????????????340?????????????????345gga?gtg?aaa?ggc?tgg?gcc?ttt?gac?gat?gga?aat?gac?gtg?tgg?atg?gga???1108Gly?Val?Lys?Gly?Trp?Ala?Phe?Asp?Asp?Gly?Asn?Asp?Val?Trp?Met?Gly

350?????????????????355?????????????????360aga?acg?atc?agc?gag?gat?tca?cgc?tca?ggt?tat?gaa?acc?ttc?aaa?gtc???1156Arg?Thr?Ile?Ser?Glu?Asp?Ser?Arg?Ser?Gly?Tyr?Glu?Thr?Phe?Lys?Val

365?????????????????370?????????????????375att?ggt?ggt?tgg?tcc?aca?cct?aat?tcc?aaa?ttg?cag?ata?aat?agg?caa???1204Ile?Gly?Gly?Trp?Ser?Thr?Pro?Asn?Ser?Lys?Leu?Gln?Ile?Asn?Arg?Gln380?????????????????385?????????????????390?????????????????395gtc?ata?gtt?gac?agc?gat?aat?agg?tca?ggt?tat?tct?ggt?att?ttc?tct???1252Val?Ile?Val?Asp?Ser?Asp?Asn?Arg?Ser?Gly?Tyr?Ser?Gly?Ile?Phe?Ser

400?????????????????405?????????????????410gtt?gag?ggc?aaa?agc?tgc?atc?aat?agg?tgc?ttt?tat?gtg?gag?ttg?ata???1300Val?Glu?Gly?Lys?Ser?Cys?Ile?Asn?Arg?Cys?Phe?Tyr?Val?Glu?Leu?Ile

415?????????????????420?????????????????425agg?gga?agg?gaa?cag?gaa?act?aga?gta?tgg?tgg?acc?tca?aac?agt?att???1348Arg?Gly?Arg?Glu?Gln?Glu?Thr?Arg?Val?Trp?Trp?Thr?Ser?Asn?Ser?Ile

430?????????????????435?????????????????440gtt?gtg?ttt?tgt?ggc?act?tca?ggt?acc?tat?ggg?aca?ggc?tca?tgg?cct???1396Val?Val?Phe?Cys?Gly?Thr?Ser?Gly?Thr?Tyr?Gly?Thr?Gly?Ser?Trp?Pro

445 450 455gat ggg gcg gac atc aat ctc atg cct ata taagctttcg caattttaga 1446Asp Gly Ala Asp Ile Asn Leu Met Pro Ile460 465aaaaactcct tgtttctact 1466＜210〉12＜211〉469＜212〉PRT＜213〉influenza A virus＜400〉12Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 15 10 15Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr

20??????????????????25??????????????????30Val?Thr?Leu?His?Phe?Lys?Gln?Tyr?Glu?Cys?Asp?Ser?Pro?Ala?Asn?Asn

35??????????????????40??????????????????45Gln?Val?Met?Pro?Cys?Glu?Pro?Ile?Ile?Ile?Glu?Arg?Asn?Ile?Thr?Glu

50??????????????????55?????????????????60Ile?Val?Tyr?Leu?Thr?Asn?Thr?Thr?Ile?Glu?Lys?Glu?Ile?Cys?Pro?Lys?65??????????????????70??????????????????75??????????????????80Leu?Val?Glu?Tyr?Arg?Asn?Trp?Ser?Lys?Pro?Gln?Cys?Lys?Ile?Thr?Gly

85??????????????????90??????????????????95Phe?Ala?Pro?Phe?Ser?Lys?Asp?Asn?Ser?Ile?Arg?Leu?Ser?Ala?Gly?Gly

100?????????????????105?????????????????110Asp?Ile?Trp?Val?Thr?Arg?Glu?Pro?Tyr?Val?Ser?Cys?Asp?Pro?Gly?Lys

115?????????????????120?????????????????125Cys?Tyr?Gln?Phe?Ala?Leu?Gly?Gln?Gly?Thr?Thr?Leu?Asp?Asn?Lys?His

130?????????????????135?????????????????140Ser?Asn?Asp?Thr?Ile?His?Asp?Arg?Thr?Pro?His?Arg?Thr?Leu?Leu?Met145?????????????????150?????????????????155?????????????????160Asn?Glu?Leu?Gly?Val?Pro?Phe?His?Leu?Gly?Thr?Arg?Gln?Val?Cys?Ile

165?????????????????170?????????????????175Ala?Trp?Ser?Ser?Ser?Ser?Cys?His?Asp?Gly?Lys?Ala?Trp?Leu?His?Val

180?????????????????185?????????????????190Cys?Val?Thr?Gly?Tyr?Asp?Lys?Asn?Ala?Thr?Ala?Ser?Phe?Ile?Tyr?Asp

195?????????????????200?????????????????205Gly?Arg?Leu?Val?Asp?Ser?Ile?Gly?Ser?Trp?Ser?Gln?Asn?Ile?Leu?Arg

210?????????????????215?????????????????220Thr?Gln?Glu?Ser?Glu?Cys?Val?Cys?Ile?Asn?Gly?Thr?Cys?Thr?Val?Val225?????????????????230?????????????????235?????????????????240Met?Thr?Asp?Gly?Ser?Ala?Ser?Gly?Arg?Ala?Asp?Thr?Lys?Ile?Leu?Phe

245?????????????????250?????????????????255Ile?Glu?Glu?Gly?Lys?Ile?Val?His?Ile?Ser?Pro?Leu?Ser?Gly?Ser?Ala

260?????????????????265?????????????????270Gln?His?Val?Glu?Glu?Cys?Ser?Cys?Tyr?Pro?Arg?Tyr?Pro?Gly?Val?Arg

275?????????????????280?????????????????285Cys?Ile?Cys?Arg?Asp?Asn?Trp?Lys?Gly?Ser?Asn?Arg?Pro?Val?Val?Asp

290?????????????????295?????????????????300Ile?Asn?Val?Lys?Asp?Tyr?Ser?Ile?Asp?Ser?Ser?Tyr?Val?Cys?Ser?Gly305?????????????????310?????????????????315?????????????????320Leu?Val?Gly?Asp?Ala?Pro?Arg?Asn?Asn?Asp?Arg?Ser?Ser?Asn?Set?Tyr

325?????????????????330?????????????????335Cys?Arg?Asn?Pro?Asn?Asn?Glu?Lys?Gly?Asn?His?Gly?Val?Lys?Gly?Trp

340?????????????????345?????????????????350Ala?Phe?Asp?Asp?Gly?Asn?Asp?Val?Trp?Met?Gly?Arg?Thr?Ile?Ser?Glu

355?????????????????360?????????????????365Asp?Ser?Arg?Ser?Gly?Tyr?Glu?Thr?Phe?Lys?Val?Ile?Gly?Gly?Trp?Ser

370?????????????????375?????????????????380Thr?Pro?Asn?Ser?Lys?Leu?Gln?Ile?Asn?Arg?Gln?Val?Ile?Val?Asp?Ser385?????????????????390?????????????????395?????????????????400Asp?Asn?Arg?Ser?Gly?Tyr?Ser?Gly?Ile?Phe?Ser?Val?Glu?Gly?Lys?Ser

405?????????????????410?????????????????415Cys?Ile?Asn?Arg?Cys?Phe?Tyr?Val?Glu?Leu?Ile?Arg?Gly?Arg?Glu?Gln

420?????????????????425?????????????????430Glu?Thr?Arg?Val?Trp?Trp?Thr?Ser?Asn?Ser?Ile?Val?Val?Phe?Cys?Gly

435?????????????????440?????????????????445Thr?Ser?Gly?Thr?Tyr?Gly?Thr?Gly?Ser?Trp?Pro?Asp?Gly?Ala?Asp?Ile

450 455 460Asn Leu Met Pro Ile465＜210〉13＜211〉1027＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(26) .. (781)＜400〉13agcaaaagca ggtagatatt gaaag atg agc ctt ctg acc gag gtc gaa acg 52

Met?Ser?Leu?Leu?Thr?Glu?Val?Glu?Thr

1???????????????5tat?gtt?ctc?tct?atc?gtt?ccg?tea?ggc?ccc?ctc?aaa?gcc?gag?atc?gcg???100Tyr?Val?Leu?Ser?Ile?Val?Pro?Ser?Gly?Pro?Leu?Lys?Ala?Glu?Ile?Ala?10??????????????????15??????????????????20??????????????????25cag?aga?ctt?gaa?gat?gtc?ttt?gct?gga?aag?aac?aca?gat?ctt?gag?gct???148Gln?Arg?Leu?Glu?Asp?Val?Phe?Ala?Gly?Lys?Asn?Thr?Asp?Leu?Glu?Ala

30??????????????????35??????????????????40ctc?atg?gaa?tgg?cta?aag?aca?aga?cca?atc?ctg?tca?cct?ctg?act?aaa???196Leu?Met?Glu?Trp?Leu?Lys?Thr?Arg?Pro?Ile?Leu?Ser?Pro?Leu?Thr?Lys

45??????????????????50??????????????????55ggg?att?ttg?gga?ttt?gta?ttc?acg?ctc?acc?gtg?ccc?agt?gag?cga?gga???244Gly?Ile?Leu?Gly?Phe?Val?Phe?Thr?Leu?Thr?Val?Pro?Ser?Glu?Arg?Gly

60??????????????????65??????????????????70ctg?cag?cgt?aga?cgc?ttt?gtc?caa?aat?gcc?ctc?aat?ggg?aat?ggg?gat???292Leu?Gln?Arg?Arg?Arg?Phe?Val?Gln?Asn?Ala?Leu?Asn?Gly?Asn?Gly?Asp

75??????????????????80??????????????????85cca?aat?aac?atg?gac?aga?gca?gtt?aaa?ctg?tat?aga?aaa?ctt?aag?agg???340Pro?Asn?Asn?Met?Asp?Arg?Ala?Val?Lys?Leu?Tyr?Arg?Lys?Leu?Lys?Arg?90??????????????????95?????????????????100?????????????????105gag?ata?aca?ttc?cat?ggg?gcc?aaa?gaa?ata?gca?ctc?agt?tat?tct?gct???388Glu?Ile?Thr?Phe?His?Gly?Ala?Lys?Glu?Ile?Ala?Leu?Ser?Tyr?Ser?Ala

110?????????????????115?????????????????120ggt?gca?ctt?gcc?agt?tgc?atg?ggc?ctc?ata?tac?aac?agg?atg?ggg?gct???436Gly?Ala?Leu?Ala?Ser?Cys?Met?Gly?Leu?Ile?Tyr?Asn?Arg?Met?Gly?Ala

125?????????????????130?????????????????135gtg?acc?act?gaa?gtg?gcc?ttt?ggc?ctg?gtt?tgt?gca?acc?tgt?gaa?cag???484Val?Thr?Thr?Glu?Val?Ala?Phe?Gly?Leu?Val?Cys?Ala?Thr?Cys?Glu?Gln

140?????????????????145?????????????????150att?gct?gac?tcc?cag?cac?agg?tct?cat?agg?caa?atg?gtg?gca?aca?acc???532Ile?Ala?Asp?Ser?Gln?His?Arg?Ser?His?Arg?Gln?Met?Val?Ala?Thr?Thr

155?????????????????160?????????????????165aat?cca?cta?ata?aga?cat?gag?aac?aga?atg?gtt?ctg?gcc?agc?act?aca???580Asn?Pro?Leu?Ile?Arg?His?Glu?Asn?Arg?Met?Val?Leu?Ala?Ser?Thr?Thr170?????????????????175?????????????????180?????????????????185gct?aag?gct?atg?gag?caa?atg?gct?gga?tca?agt?gag?cag?gca?gca?gag???628Ala?Lys?Ala?Met?Glu?Gln?Met?Ala?Gly?Ser?Ser?Glu?Gln?Ala?Ala?Glu

190?????????????????195?????????????????200gcc?atg?gag?gtt?gct?agt?cag?gcc?agg?caa?atg?gtg?cag?gca?atg?aga???676Ala?Met?Glu?Val?Ala?Ser?Gln?Ala?Arg?Gln?Met?Val?Gln?Ala?Met?Arg

205?????????????????210?????????????????215gcc?att?ggg?act?cat?cct?agc?tcc?agt?gct?ggt?cta?aaa?gat?gat?ctt???724Ala?Ile?Gly?Thr?His?Pro?Ser?Ser?Ser?Ala?Gly?Leu?Lys?Asp?Asp?Leu

220?????????????????225?????????????????230ctt?gaa?aat?ttg?cag?gcc?tat?cag?aaa?cga?atg?ggg?gtg?cag?atg?caa???772Leu?Glu?Asn?Leu?Gln?Ala?Tyr?Gln?Lys?Arg?Met?Gly?Val?Gln?Met?Gln

235 240 245cga ttc aag tgaccctctt gttgttgctg cgagtatcat tgggatcttg 821Arg Phe Lys250cacttgatat tgtggattct tgatcgtctt tttttcaaat gcatctatcg attctttgaa 881cacggtctga aaagagggcc ttctacggaa ggagtacctg agtctatgag ggaagaatat 941cgaaaggaac agcagagtgc tgtggatgct gacgacagtc attttgtcag catagagctg 1001gagtaaaaaa ctaccttgtt tctact 1027 <210> 14 <211> 252 <212> PRT <213> Influenza A virus <400> 14Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Val Pro 1 5 l0 15Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe

20??????????????????25??????????????????30Ala?Gly?Lys?Asn?Thr?Asp?Leu?Glu?Ala?Leu?Met?Glu?Trp?Leu?Lys?Thr

35??????????????????40??????????????????45Arg?Pro?Ile?Leu?Ser?Pro?Leu?Thr?Lys?Gly?Ile?Leu?Gly?Phe?Val?Phe

50??????????????????55??????????????????60?Thr?Leu?Thr?Val?Pro?Ser?Glu?Arg?Gly?Leu?Gln?Arg?Arg?Arg?Phe?Val?65??????????????????70??????????????????75??????????????????80Gln?Asn?Ala?Leu?Asn?Gly?Asn?Gly?Asp?Pro?Asn?Asn?Met?Asp?Arg?Ala

85??????????????????90??????????????????95Val?Lys?Leu?Tyr?Arg?Lys?Leu?Lys?Arg?Glu?Ile?Thr?Phe?His?Gly?Ala

100?????????????????105?????????????????110Lys?Glu?Ile?Ala?Leu?Ser?Tyr?Ser?Ala?Gly?Ala?Leu?Ala?Ser?Cys?Met

115?????????????????120?????????????????125Gly?Leu?Ile?Tyr?Asn?Arg?Met?Gly?Ala?Val?Thr?Thr?Glu?Val?Ala?Phe

130?????????????????135?????????????????140Gly?Leu?Val?Cys?Ala?Thr?Cys?Glu?Gln?Ile?Ala?Asp?Ser?Gln?His?Arg145?????????????????150?????????????????155?????????????????160Ser?His?Arg?Gln?Met?Val?Ala?Thr?Thr?Asn?Pro?Leu?Ile?Arg?His?Glu

165?????????????????170?????????????????175Asn?Arg?Met?Val?Leu?Ala?Ser?Thr?Thr?Ala?Lys?Ala?Met?Glu?Gln?Met

180?????????????????185?????????????????190Ala?Gly?Ser?Ser?Glu?Gln?Ala?Ala?Glu?Ala?Met?Glu?Val?Ala?Ser?Gln

195?????????????????200?????????????????205Ala?Arg?Gln?Met?Val?Gln?Ala?Met?Arg?Ala?Ile?Gly?Thr?His?Pro?Ser

2l0?????????????????215?????????????????220Ser?Ser?Ala?Gly?Leu?Lys?Asp?Asp?Leu?Leu?Glu?Asn?Leu?Gln?Ala?Tyr225?????????????????230?????????????????235?????????????????240Gln?Lys?Arg?Met?Gly?Val?Gln?Met?Gln?Arg?Phe?Lys

245 250＜210〉15＜211〉322＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(26) .. (316)＜400〉15agcaaaagca ggtagatatt gaaag atg agc ctt ctg acc gag gtc gaa acg 52

Met?Ser?Leu?Leu?Thr?Glu?Val?Glu?Thr

1???????????????5cct?atc?aga?aac?gaa?tgg?ggg?tgc?aga?tgc?aac?gat?tca?agt?gac?cct???100Pro?Ile?Arg?Asn?Glu?Trp?Gly?Cys?Arg?Cys?Asn?Asp?Ser?Ser?Asp?Pro?10??????????????????15??????????????????20??????????????????25ctt?gtt?gtt?gct?gcg?agt?atc?att?ggg?atc?ttg?cac?ttg?ata?ttg?tgg???148Leu?Val?Val?Ala?Ala?Ser?Ile?Ile?Gly?Ile?Leu?His?Leu?Ile?Leu?Trp

30??????????????????35??????????????????40att?ctt?gat?cgt?ctt?ttt?ttc?aaa?tgc?atc?tat?cga?ttc?ttt?gaa?cac???196Ile?Leu?Asp?Arg?Leu?Phe?Phe?Lys?Cys?Ile?Tyr?Arg?Phe?Phe?Glu?His

45??????????????????50??????????????????55ggt?ctg?aaa?aga?ggg?cct?tct?acg?gaa?gga?gta?cct?gag?tct?atg?agg???244Gly?Leu?Lys?Arg?Gly?Pro?Ser?Thr?Glu?Gly?Val?Pro?Glu?Ser?Met?Arg

60??????????????????65??????????????????70gaa?gaa?tat?cga?aag?gaa?cag?cag?agt?gct?gtg?gat?gct?gac?gac?agt???292Glu?Glu?Tyr?Arg?Lys?Glu?Gln?Gln?Ser?Ala?Val?Asp?Ala?Asp?Asp?Ser

75 80 85cat ttt gtc agc ata gag ctg gag taaaaa 322His Phe Val Ser Ile Glu Leu Glu 90 95＜210〉16＜211〉97＜212〉PRT＜213〉influenza A virus＜400〉16Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 15 10 15Cys Arg Cys Asn Asp Ser Ser Asp Pro Leu Val Val Ala Ala Ser Ile

20??????????????????25??????????????????30Ile?Gly?Ile?Leu?His?Leu?Ile?Leu?Trp?Ile?Leu?Asp?Arg?Leu?Phe?Phe

35??????????????????40??????????????????45Lys?Cys?Ile?Tyr?Arg?Phe?Phe?Glu?His?Gly?Leu?Lys?Arg?Gly?Pro?Ser

50??????????????????55??????????????????60Thr?Glu?Gly?Val?Pro?Glu?Ser?Met?Arg?Glu?Glu?Tyr?Arg?Lys?Glu?Gln?65??????????????????70??????????????????75??????????????????80Gln?Ser?Ala?Val?Asp?Ala?Asp?Asp?Ser?His?Phe?Val?Ser?Ile?Glu?Leu

85 90 95Glu＜210〉17＜211〉890＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(27) .. (737)＜400〉17agcaaaagca gggtgacaaa gacata atg gat tcc aac act gtg tca agt ttt 53

Met?Asp?Ser?Asn?Thr?Val?Ser?Ser?Phe

1???????????????5cag?gta?gac?tgc?ttc?ctt?tgg?cat?gtc?cga?aaa?caa?gtt?gta?gac?caa??101Gln?Val?Asp?Cys?Phe?Leu?Trp?His?Val?Arg?Lys?Gln?Val?Val?Asp?Gln?10??????????????????15??????????????????20??????????????????25gaa?cta?ggt?gat?gcc?cca?ttc?ctt?gat?cgg?ctt?cgc?cga?gat?cag?aag??149Glu?Leu?Gly?Asp?Ala?Pro?Phe?Leu?Asp?Arg?Leu?Arg?Arg?Asp?Gln?Lys

30??????????????????35??????????????????40tcc?cta?agg?gga?aga?ggc?agc?act?ctc?ggt?cta?aac?atc?gaa?gca?gcc??197Ser?Leu?Arg?Gly?Arg?Gly?Ser?Thr?Leu?Gly?Leu?Asn?Ile?Glu?Ala?Ala

45??????????????????50??????????????????55acc?cat?gtt?gga?aag?cag?ata?gta?gag?aag?att?ctg?aag?gaa?gaa?tct??245Thr?His?Val?Gly?Lys?Gln?Ile?Val?Glu?Lys?Ile?Leu?Lys?Glu?Glu?Ser

60??????????????????65??????????????????70gat?gag?gca?ctt?aaa?atg?acc?atg?gcc?tcc?aca?cct?gct?tcg?cga?tac??293Asp?Glu?Ala?Leu?Lys?Met?Thr?Met?Ala?Ser?Thr?Pro?Ala?Ser?Arg?Tyr

75??????????????????80??????????????????85ata?act?gac?atg?act?att?gag?gaa?ttg?tca?agg?gac?tgg?ttc?atg?cta???341Ile?Thr?Asp?Met?Thr?Ile?Glu?Glu?Leu?Ser?Arg?Asp?Trp?Phe?Met?Leu?90??????????????????95?????????????????100?????????????????105atg?ccc?aag?cag?aaa?gtg?gaa?gga?cct?ctt?tgc?atc?aga?ata?gac?caa???389Met?Pro?Lys?Gln?Lys?Val?Glu?Gly?Pro?Leu?Cys?Ile?Arg?Ile?Asp?Gln

110?????????????????115?????????????????120gca?atc?atg?gat?aag?aac?atc?atg?ttg?aaa?gcg?aat?ttc?agt?gtg?att???437Ala?Ile?Met?Asp?Lys?Asn?Ile?Met?Leu?Lys?Ala?Asn?Phe?Ser?Val?Ile

125?????????????????130?????????????????135ttt?gac?cgg?cta?gag?acc?cta?ata?tta?cta?agg?gct?ttc?acc?gaa?gag???485Phe?Asp?Arg?Leu?Glu?Thr?Leu?Ile?Leu?Leu?Arg?Ala?Phe?Thr?Glu?Glu

140?????????????????145?????????????????150gga?gca?att?gtt?ggc?gaa?atc?tca?cca?ttg?cct?tct?ttt?cca?gga?cat???533Gly?Ala?Ile?Val?Gly?Glu?Ile?Ser?Pro?Leu?Pro?Ser?Phe?Pro?Gly?His

155?????????????????160?????????????????165act?att?gag?gat?gtc?aaa?aat?gca?att?ggg?gtc?ctc?atc?gga?gga?ctt???581Thr?Ile?Glu?Asp?Val?Lys?Asn?Ala?Ile?Gly?Val?Leu?Ile?Gly?Gly?Leu170?????????????????175?????????????????180?????????????????185gaa?tgg?aat?gat?aac?aca?gtt?cga?gtc?tct?aaa?act?cta?cag?aga?ttc???629Glu?Trp?Asn?Asp?Asn?Thr?Val?Arg?Val?Ser?Lys?Thr?Leu?Gln?Arg?Phe

190?????????????????195?????????????????200gct?tgg?gga?agc?agt?aat?gag?aat?ggg?aga?cct?cca?ctt?act?cca?aaa???677Ala?Trp?Gly?Ser?Ser?Asn?Glu?Asn?Gly?Arg?Pro?Pro?Leu?Thr?Pro?Lys

205?????????????????210?????????????????215cag?aaa?cgg?aaa?atg?gcg?aga?aca?gct?agg?tca?aaa?gtt?cga?aga?gat???725Gln?Lys?Arg?Lys?Met?Ala?Arg?Thr?Ala?Arg?Ser?Lys?Val?Arg?Arg?Asp

220?????????????????225?????????????????230aag?atg?gct?gat?tgaagaagtg?agacacagac?tgaagacaac?agagaatagt???????777Lys?Met?Ala?Asp

235tttgagcaaa taacattcat gcaagcctta cagctactat ttgaagtgga acaggagata 837agaactttct cgtttcagct tatttaatga taaaaaacac ccttgtttct act 890＜210〉18＜211〉237＜212〉PRT＜213〉influenza A virus＜400〉18Met Asp Ser Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 15 10 15 His Val Arg Lys Gln Val Val Asp Gln Glu Leu Gly Asp Ala Pro Phe

20??????????????????25??????????????????30Leu?Asp?Arg?Leu?Arg?Arg?Asp?Gln?Lys?Ser?Leu?Arg?Gly?Arg?Gly?Ser

35??????????????????40??????????????????45Thr?Leu?Gly?Leu?Asn?Ile?Glu?Ala?Ala?Thr?His?Val?Gly?Lys?Gln?Ile

50??????????????????55??????????????????60Val?Glu?Lys?Ile?Leu?Lys?Glu?Glu?Ser?Asp?Glu?Ala?Leu?Lys?Met?Thr?65??????????????????70??????????????????75??????????????????80Met?Ala?Ser?Thr?Pro?Ala?Ser?Arg?Tyr?Ile?Thr?Asp?Met?Thr?Ile?Glu

85??????????????????90??????????????????95Glu?Leu?Ser?Arg?Asp?Trp?Phe?Met?Leu?Met?Pro?Lys?Gln?Lys?Val?Glu

100?????????????????105?????????????????110Gly?Pro?Leu?Cys?Ile?Arg?Ile?Asp?Gln?Ala?Ile?Met?Asp?Lys?Asn?Ile

115?????????????????120?????????????????125Met?Leu?Lys?Ala?Asn?Phe?Ser?Val?Ile?Phe?Asp?Arg?Leu?Glu?Thr?Leu

130?????????????????135?????????????????140Ile?Leu?Leu?Arg?Ala?Phe?Thr?Glu?Glu?Gly?Ala?Ile?Val?Gly?Glu?Ile145?????????????????150?????????????????155?????????????????160Ser?Pro?Leu?Pro?Ser?Phe?Pro?Gly?His?Thr?Ile?Glu?Asp?Val?Lys?Asn

165?????????????????170?????????????????175Ala?Ile?Gly?Val?Leu?Ile?Gly?Gly?Leu?Glu?Trp?Asn?Asp?Asn?Thr?Val

180?????????????????185?????????????????190Arg?Val?Ser?Lys?Thr?Leu?Gln?Arg?Phe?Ala?Trp?Gly?Ser?Ser?Asn?Glu

195?????????????????200?????????????????205Asn?Gly?Arg?Pro?Pro?Leu?Thr?Pro?Lys?Gln?Lys?Arg?Lys?Met?Ala?Arg

210 215 220Thr Ala Arg Ser Lys Val Arg Arg Asp Lys Met Ala Asp225 230 235＜210〉19＜211〉402＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(27) .. (389)＜400〉19agcaaaagca gggtgacaaa gacata atg gat tcc aac act gtg tca agt ttt 53

Met?Asp?Ser?Asn?Thr?Val?Ser?Ser?Phe

1???????????????5cag?gac?ata?cta?ttg?agg?atg?tca?aaa?atg?caa?ttg?ggg?tcc?tca?tcg???101Gln?Asp?Ile?Leu?Leu?Arg?Met?Ser?Lys?Met?Gln?Leu?Gly?Ser?Ser?Ser?10??????????????????15??????????????????20??????????????????25gag?gac?ttg?aat?gga?atg?ata?aca?cag?ttc?gag?tct?cta?aaa?ctc?tac???149Glu?Asp?Leu?Asn?Gly?Met?Ile?Thr?Gln?Phe?Glu?Ser?Leu?Lys?Leu?Tyr

30??????????????????35??????????????????40aga?gat?tcg?ctt?ggg?gaa?gca?gta?atg?aga?atg?gga?gac?ctc?cac?tta???197Arg?Asp?Ser?Leu?Gly?Glu?Ala?Val?Met?Arg?Met?Gly?Asp?Leu?His?Leu

45??????????????????50??????????????????55ctc?caa?aac?aga?aac?gga?aaa?tgg?cga?gaa?cag?cta?ggt?caa?aag?ttc???245Leu?Gln?Asn?Arg?Asn?Gly?Lys?Trp?Arg?Glu?Gln?Leu?Gly?Gln?Lys?Phe

60??????????????????65??????????????????70gaa?gag?ata?aga?tgg?ctg?att?gaa?gaa?gtg?aga?cac?aga?ctg?aag?aca???293Glu?Glu?Ile?Arg?Trp?Leu?Ile?Glu?Glu?Val?Arg?His?Arg?Leu?Lys?Thr

75??????????????????80??????????????????85aca?gag?aat?agt?ttt?gag?caa?ata?aca?ttc?atg?caa?gcc?tta?cag?cta???341Thr?Glu?Asn?Ser?Phe?Glu?Gln?Ile?Thr?Phe?Met?Gln?Ala?Leu?Gln?Leu?90??????????????????95?????????????????100?????????????????105cta?ttt?gaa?gtg?gaa?cag?gag?ata?aga?act?ttc?tcg?ttt?cag?ctt?att???389Leu?Phe?Glu?Val?Glu?Gln?Glu?Ile?Arg?Thr?Phe?Ser?Phe?Gln?Leu?Ile

1l0 115 120taatgataaa aaa 402＜210〉20＜211〉121＜212〉PRT＜213〉influenza A virus＜400〉20Met Asp Ser Asn Thr Val Ser Ser Phe Gln Asp Ile Leu Leu Arg Met 15 10 15Ser Lys Met Gln Leu Gly Ser Ser Ser Glu Asp Leu Asn Gly Met Ile

20??????????????????25??????????????????30Thr?Gln?Phe?Glu?Ser?Leu?Lys?Leu?Tyr?Arg?Asp?Ser?Leu?Gly?Glu?Ala

35??????????????????40??????????????????45Val?Met?Arg?Met?Gly?Asp?Leu?His?Leu?Leu?Gln?Asn?Arg?Asn?Gly?Lys

50??????????????????55??????????????????60Trp?Arg?Glu?Gln?Leu?Gly?Gln?Lys?Phe?Glu?Glu?Ile?Arg?Trp?Leu?Ile?65??????????????????70??????????????????75??????????????????80Glu?Glu?Val?Arg?His?Arg?Leu?Lys?Thr?Thr?Glu?Asn?Ser?Phe?Glu?Gln

85??????????????????90??????????????????95Ile?Thr?Phe?Met?Gln?Ala?Leu?Gln?Leu?Leu?Phe?Glu?Val?Glu?Gln?Glu

100?????????????????105?????????????????110Ile?Arg?Thr?Phe?Ser?Phe?Gln?Leu?Ile

115 120＜210〉21＜211〉1764＜212〉DNA＜213〉influenza A virus＜220〉＜221〉CDS＜222〉(30) .. (1727)＜400〉21agcaaaagca ggggataatt ctattaacc atg aaa act atc att gct ttg agc 53

Met?Lys?Thr?Ile?Ile?Ala?Leu?Ser

1???????????????5tac?att?ttc?tgt?ctg?gtt?ctc?ggc?caa?gac?ttt?cca?gga?aat?gac?aac???101Tyr?Ile?Phe?Cys?Leu?Val?Leu?Gly?Gln?Asp?Phe?Pro?Gly?Asn?Asp?Asn

250?????????????????255?????????????????260ggg?aac?cta?att?gct?cct?cgg?ggt?tat?ttt?aaa?atg?cgc?act?ggg?aaa???869Gly?Asn?Leu?Ile?Ala?Pro?Ara?Gly?Tyr?Phe?Lys?Met?Arg?Thr?Gly?Lys265?????????????????270?????????????????275?????????????????280agc?tca?ata?atg?agg?tca?gat?gca?cct?att?ggc?acc?tgc?att?tct?gaa???917Ser?Ser?Ile?Met?Arg?Ser?Asp?Ala?Pro?Ile?Gly?Thr?Cys?Ile?Ser?Glu

330?????????????????335?????????????????340aga?ggc?cta?ttc?agc?gca?ata?gca?ggt?ttc?ata?gaa?aat?ggt?tgg?gag???1109Arg?Gly?Leu?Phe?Ser?Ala?Ile?Ala?Gly?Phe?Ile?Glu?Asn?Gly?Trp?Glu345?????????????????350?????????????????355?????????????????360gga?atg?ata?gac?ggt?tgg?tac?ggt?ttc?agg?cat?caa?aat?tct?gag?ggc???1157Gly?Met?Ile?Asp?Gly?Trp?Tyr?Gly?Phe?Arg?His?Gln?Asn?Ser?Glu?Gly

365?????????????????370?????????????????375aca?gga?caa?gca?gca?gat?ctt?aaa?age?act?caa?gca?gcc?atc?gac?caa???1205Thr?Gly?Gln?Ala?Ala?Asp?Leu?Lys?Ser?Thr?Gln?Ala?Ala?Ile?Asp?Gln

410?????????????????415?????????????????420ctc?gag?aaa?tac?gtt?gaa?gac?act?aaa?ata?gat?ctc?tgg?tct?tac?aat???1349Leu?Glu?Lys?Tyr?Val?Glu?Asp?Thr?Lys?Ile?Asp?Leu?Trp?Ser?Tyr?Asn425?????????????????430?????????????????435?????????????????440gcg?gag?ctt?ctt?gtc?gct?ctg?gag?aac?caa?cat?aca?att?gat?ctg?act???1397Ala?Glu?Leu?Leu?Val?Ala?Leu?Glu?Asn?Gln?His?Thr?Ile?Asp?Leu?Thr

460?????????????????465?????????????????470gaa?aat?gct?gag?gac?atg?ggc?aat?ggt?tgc?ttc?aaa?ata?tac?cac?aaa???1493Glu?Asn?Ala?Glu?Asp?Met?Gly?Asn?Gly?Cys?Phe?Lys?Ile?Tyr?His?Lys

490?????????????????495?????????????????500gat?gta?tac?aga?gac?gaa?gca?tta?aac?aac?cgg?ttt?cag?atc?aaa?ggt???1589Asp?Val?Tyr?Arg?Asp?Glu?Ala?Leu?Asn?Asn?Arg?Phe?Gln?Ile?Lys?Gly505?????????????????510?????????????????515?????????????????520gtt?gaa?ctg?aag?tca?gga?tac?aaa?gac?tgg?atc?ctg?tgg?att?tcc?ttt???1637Val?Glu?Leu?Lys?Ser?Gly?Tyr?Lys?Asp?Trp?Ile?Leu?Trp?Ile?Ser?Phe

555 560 565tgagtgtatt agtaattaaa aacaccctgt ttctact 1764＜210〉22＜211〉566＜212〉PRT＜213〉influenza A virus＜400〉22Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Leu Gly 15 10 15Gln Asp Phe Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

450?????????????????455?????????????????460Glu?Lys?Thr?Arg?Arg?Gln?Leu?Arg?Glu?Asn?Ala?Glu?Asp?Met?Gly?Asn465?????????????????470?????????????????475?????????????????480Gly?Cys?Phe?Lys?Ile?Tyr?His?Lys?Cys?Asp?Asn?Ala?Cys?Ile?Gly?Ser

515?????????????????520?????????????????525Asp?Trp?Ile?Leu?Trp?Ile?Ser?Phe?Ala?Ile?Ser?Cys?Phe?Leu?Leu?Cys

565 <210> 23 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 23 tatggaaaga ataaaagaac tacggaa 27 <210> 24 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 24 tcgtttttaa actattcaac at 22 <210> 25 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 25 agcaggtcaa ttatattcaa tatg 24 <210> 26 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 26 aacaaggtcg tttttaaact attc 24 <210> 27 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 27 agaactctat tccaacaaat g 21 <210> 28 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 28 aatcggatat ttcattgcca t 21 <210> 29 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 29 agaactctat tccaacaaat gagggatgta gtt 33 <210> 30 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 30 aatcggatat ttcattgcca tcatccattt cat 33 <210> 31 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 31 agacgtggtg ttggtaatga a 21 <210> 32 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 32 cgaagagttg acataaaccc t 21 <210> 33 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 33 tcatccctca tcccctcaca t 21 <210> 34 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 34 attttctgtt atcctcttgt ca 22 <210> 35 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 35 gtggagtccg ctgttttgag 20 <210> 36 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 36 aggatggtgg acattcttag g 21 <210> 37 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 37 ccgatcaatg ctaaccacta c 21 <210> 38 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 38 agcaaaagca ggtcaattat attca 25 <210> 39 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 39 agtagaaaca aggtcgtttt taaac 25 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 40 agcaaaagca ggcaaaccat 20 <210> 41 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 41 agtagaaaca aggcattttt t 21 <210> 42 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 42 tcgagctgaa gaagctatgg 20 <210> 43 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 43 gttctgttga ctgtgtccat 20 <210> 44 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 44 tcgagctgaa gaagctatgg gagcagaccc gt 32 <210> 45 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 45 gttctgttga ctgtgtccat ggtgtatcc 29 <210> 46 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 46 agcgaaagca ggcaaaccat ttgaatggat gtcaa 35 <210> 47 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 47 attaaaaaca aggcattttt tcatgaagga c 31 <210> 48 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 48 aatttccagc atggtggagg ccatggtg 28 <210> 49 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 49 gggatctttg aaaactcgtg 20 <210> 50 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 50 cagcattgtt tacagactc 19 <210> 51 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 51 accaaagatg cagaaagagg 20 <210> 52 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 52 ctcatattga ttccgactaa 20 <210> 53 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 53 tgtccacttc cctttttctg a 21 <210> 54 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 54 atttttcccc agtagttcat ac 22 <210> 55 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 55 acgctgttgc aactacacac tcctg 25 <210> 56 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 56 agcaaaagca ggtactgatt cgaga 25 <210> 57 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 57 agtagaaaca aggtactttt ttgga 25 <210> 58 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 58 gaacctggaa cctttgatct t 21 <210> 59 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 59 attcaccact gtccaggcca t 21 <210> 60 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 60 gaacctggaa cctttgatct tgaggggcta 30 <210> 61 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 61 attcaccact gtccaggcca ttgacgcgtc 30 <210> 62 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 62 agcaaaagca ggtagtgata g 21 <210> 63 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 63 agtagaaaca aggta 15 <210> 64 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 64 agcgaaagca ggtagtgatt cgagatgga 29 <210> 65 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 65 agtagaaaca aggtactttt ttggacag 28 <210> 66 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 66 tcgatttgtt ggagtgactg a 21 <210> 67 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 67 gccgaacttc tcctgccttg a 21 <210> 68 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 68 gtattcaata gcctgtatg 19 <210> 69 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 69 gacttctctc cttgtcactc 20 <210> 70 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 70 acgagtcagc taaagtgggc a 21 <210> 71 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 71 gacacctctg ctgtgaagta a 21 <210> 72 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 72 ggagctgaga aaccgaagtt t 21 <210> 73 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 73 gacttggcca ataaagtcct a 21 <210> 74 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 74 gcctgcgcat agttcctgtg a 21 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 75 tctaccacta ttgactcgcc 20 <210> 76 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 76 agcaaaagca ggggataatt cta 23 <210> 77 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 77 agtagaaaca agggtgtttt taa 23 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 78 acagtttgtt catttccgag 20 <210> 79 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 79 acttcagggt gttttgctta 20 <210> 80 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 80 gaacccccca aatgtatagt 20 <210> 81 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 81 tgaggaactc tgaaccagct 20 <210> 82 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 82 gtcactagtt gcctcgtcag 20 <210> 83 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 83 ccgggagaca tactggtaat 20 <210> 84 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 84 caaatcaatg ggaaactgaa 20 <210> 85 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 85 tgaactgaag tcaggataca 20 <210> 86 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 86 agcaaaagca gggttaataa tcac 24 <210> 87 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 87 agtagaaaca agggtatttt tcct 24 <210> 88 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 88 ttgcaccttc catcatcctt 20 <210> 89 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 89 gtctattccc actaaagagt 20 <210> 90 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 90 tgcatcagag agcacatcct 20 <210> 91 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 91 gaactcgtcc tttatgacaa 20 <210> 92 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 92 agagcaatgg atcaagt 17 <210> 93 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 93 tactatggaa tcaagtactc 20 <210> 94 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 94 atcaatcatc ttcccgac 18 <210> 95 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 95 agtgtccttc cgtgggcg 18 <210> 96 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 96 agcaaaagca ggagtgaaga tga 23 <210> 97 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 97 agtagaaaca aggagttttt tcta 24 <210> 98 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 98 tcgttgtttc tgggtgtgtc 20 <210> 99 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 99 ttatcatacc cagtgacaca 20 <210> 100 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 100 attattattg gttcacacgg 20 <210> 101 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 101 ttatgtgtca tgcgatcctg 20 <210> 102 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 102 attgttcata ttagcccatt 20 <210> 103 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 103 ataggtcagg ttattctggt 20 <210> 104 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 104 agcaaaagca ggtag 15 <210> 105 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 105 agtagaaaca aggta 15 <210> 106 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 106 tttactccag ctctatgctg acaa 24 <210> 107 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 107 gatccagcca tttgctccat 20 <210> 108 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 108 gaggtgacag gattggtctt 20 <210> 109 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 109 catggacaga gcagttaaac 20 <210> 110 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 110 gcgagtatca ttgggatctt 20 <210> 111 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 111 agcaaaagca gggtgacaaa gaca 24 <210> 112 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 112 agtagaaaca agggtgtttt ttat 24 <210> 113 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 113 ttttttatca ttaaataagc tgaa 24 <210> 114 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 114 aattgcattt ttgacatcct 20 <210> 115 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 115 atcttctcta ctatctgctt 20 <210> 116 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 116 caagcaatca tggataagaa 20 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 117 ggcgagaaca gctaggtcaa 20 ...

Claims

1. isolated nucleic acid molecule, it is characterized in that, the normal chain that comprises influenza A/Udorn/72 (H3N2) bacterial strain is the complete nucleotide sequence of anti-genome sense strand, described complete nucleotide sequence is made of following sequence: (a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:17, or (b) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17 and SEQ ID NO:21.

2. isolated nucleic acid molecule as claimed in claim 1, it is characterized in that, comprise the complete nucleotide sequence of influenza A/Udorn/72 (H3N2) bacterial strain, described complete sequence is made of following sequence: SEQID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:17.

3. isolated nucleic acid molecule as claimed in claim 1, it is characterized in that, comprise the complete nucleotide sequence of influenza A/Udorn/72 (H3N2) bacterial strain, described complete sequence is made of following sequence: SEQID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ IDNO:13, SEQ ID NO:17 and SEQ ID NO:21.。

4. isolated nucleic acid molecule, it is characterized in that, the normal chain that comprises single influenza A virus is the nucleotide sequence of anti-genome sense strand, and described sequence is selected from: SEQ ID NO:1, SEQ ID NO:3, SEQ IDNO:5, SEQ ID NO:7, SEQ ID NO:11 and SEQ ID NO:21.

5. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:1.

6. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:3.

7. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:5.

8. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:7.

9. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:11.

10. isolated nucleic acid molecule as claimed in claim 4 is characterized in that, described influenza A virus nucleotide sequence is SEQ ID NO:21.

11. isolated nucleic acid molecule, it is characterized in that, the normal chain that comprises single influenza A virus is the nucleotide sequence of anti-genome sense strand, and described sequence encoding protein, this protein have and is selected from following sequence: SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6.

12. isolating nucleic acid molecule as claimed in claim 11 is characterized in that, the nucleotide sequence coded sequence of described influenza A virus is the protein of SEQ ID NO:2.

13. isolating nucleic acid molecule as claimed in claim 11 is characterized in that, the nucleotide sequence coded sequence of described influenza A virus is the protein of SEQ ID NO:4.

14. isolating nucleic acid molecule as claimed in claim 11 is characterized in that, the nucleotide sequence coded sequence of described influenza A virus is the protein of SEQ ID NO:6.

15. an isolating aminoacid sequence is characterized in that, comprises single influenza A virus aminoacid sequence, described sequence is selected from: SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6.

16. isolating aminoacid sequence as claimed in claim 15 is characterized in that, the aminoacid sequence of described influenza A virus is SEQ ID NO:2.

17. isolating aminoacid sequence as claimed in claim 15 is characterized in that, the aminoacid sequence of described influenza A virus is SEQ ID NO:4.

18. isolating aminoacid sequence as claimed in claim 15 is characterized in that, the aminoacid sequence of described influenza A virus is SEQ ID NO:6.

19. an isolating aminoacid sequence, the sequence that it comprises single influenza A virus is characterized in that, described sequence is SEQ ID NO:8.

20. an isolating aminoacid sequence, the sequence that it comprises single influenza A virus is characterized in that, described sequence is SEQ ID NO:12.