CA2406356C - Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse - Google Patents

Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse Download PDF

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Publication number
CA2406356C
CA2406356C CA002406356A CA2406356A CA2406356C CA 2406356 C CA2406356 C CA 2406356C CA 002406356 A CA002406356 A CA 002406356A CA 2406356 A CA2406356 A CA 2406356A CA 2406356 C CA2406356 C CA 2406356C
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Canada
Prior art keywords
polypeptide
amino acid
mass
acid residue
reaction
Prior art date
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Expired - Lifetime
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CA002406356A
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English (en)
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CA2406356A1 (fr
Inventor
Brian T. Chait
Ronald Beavis
Rong Wang
Stephen B. H. Kent
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Rockefeller University
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Rockefeller University
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Priority claimed from CA002136717A external-priority patent/CA2136717A1/fr
Publication of CA2406356A1 publication Critical patent/CA2406356A1/fr
Application granted granted Critical
Publication of CA2406356C publication Critical patent/CA2406356C/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Une méthode est décrite pour la détermination de la séquence de polypeptides en formant des échelles de peptides comprenant une série de polypeptides dans laquelle les éléments adjacents de la série varient par un résidu d'acide aminé et déterminant l'identité et la position de chaque acide aminé dans le polypeptide à l'aide d'un spectromètre de masse.
CA002406356A 1992-05-29 1993-05-27 Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse Expired - Lifetime CA2406356C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US89117792A 1992-05-29 1992-05-29
US07/891,177 1992-05-29
CA002136717A CA2136717A1 (fr) 1992-05-29 1993-05-27 Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002136717A Division CA2136717A1 (fr) 1992-05-29 1993-05-27 Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse

Publications (2)

Publication Number Publication Date
CA2406356A1 CA2406356A1 (fr) 1993-12-09
CA2406356C true CA2406356C (fr) 2007-01-30

Family

ID=25677638

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002406356A Expired - Lifetime CA2406356C (fr) 1992-05-29 1993-05-27 Methode et produit pour la determination de la sequence de peptides a l'aide d'un spectrometre de masse

Country Status (1)

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CA (1) CA2406356C (fr)

Also Published As

Publication number Publication date
CA2406356A1 (fr) 1993-12-09

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Effective date: 20130527