CA2394614A1 - Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique - Google Patents

Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique Download PDF

Info

Publication number
CA2394614A1
CA2394614A1 CA002394614A CA2394614A CA2394614A1 CA 2394614 A1 CA2394614 A1 CA 2394614A1 CA 002394614 A CA002394614 A CA 002394614A CA 2394614 A CA2394614 A CA 2394614A CA 2394614 A1 CA2394614 A1 CA 2394614A1
Authority
CA
Canada
Prior art keywords
cells
cell
embryonic
human
oocyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002394614A
Other languages
English (en)
Inventor
Jose Cibelli
Robert Lanza
James Robl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UMass Amherst
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2394614A1 publication Critical patent/CA2394614A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de transfert nucléaire qui consiste à transplanter des noyaux de cellules donneuses différentiées dans des ovocytes énucléés d'une espèce différente de celle de la cellule donneuse, conjointement avec de l'ADN cytoplasmique et/ou mitochondrien compatible (de la même espèce que le donneur). Les unités de transfert nucléaire résultantes sont utiles pour la production de cellules souches embryonnaires isogènes, notamment de cellules souches ou embryonnaires isogènes humaines. Ces cellules embryonnaires ou souches sont utiles pour la production de cellules différentiées voulues et pour l'introduction, le retrait ou la modification de gènes voulus, par exemple dans des sites spécifiques du génome desdites cellules par recombinaison homologue. Lesdites cellules, qui peuvent contenir un gène hétérologue, sont particulièrement utiles dans les thérapies de transplantation cellulaire et pour l'étude in vitro de la différentiation cellulaire. L'efficacité du transfert nucléaire est accrue par l'introduction d'ADN cytoplasmique ou mitochondrien compatible (de même espèce ou similaire à la cellule donneuse ou au noyau donneur). Par ailleurs, l'efficacité du transfert nucléaire peut être encore éventuellement améliorée par la modification génétique des cellules donneuses pour l'inhibition de l'apoptose, par la sélection pour un cycle cellulaire spécifique et/ou par la mise en culture dans des conditions augmentant la croissance et le développement embryonnaire.
CA002394614A 1999-12-20 2000-05-10 Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique Abandoned CA2394614A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US46707699A 1999-12-20 1999-12-20
US09/467,076 1999-12-20
PCT/US2000/012631 WO2001046401A1 (fr) 1999-12-20 2000-05-10 Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique

Publications (1)

Publication Number Publication Date
CA2394614A1 true CA2394614A1 (fr) 2001-06-28

Family

ID=23854253

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002394614A Abandoned CA2394614A1 (fr) 1999-12-20 2000-05-10 Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique

Country Status (10)

Country Link
EP (1) EP1240318A4 (fr)
JP (1) JP2004500066A (fr)
CN (1) CN1425064A (fr)
AU (1) AU784731B2 (fr)
BR (1) BR0016561A (fr)
CA (1) CA2394614A1 (fr)
IL (2) IL150065A0 (fr)
MX (1) MXPA02006093A (fr)
NZ (1) NZ519346A (fr)
WO (1) WO2001046401A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9550974B2 (en) 2004-11-04 2017-01-24 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US9617512B2 (en) 2004-11-04 2017-04-11 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells and embryo-derived cells
US10584313B2 (en) 2007-02-23 2020-03-10 Astellas Institute For Regenerative Medicine Method of producing a differentiated mammalian cell comprising culturing a single mammalian blastomere

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7696404B2 (en) 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
AU782286B2 (en) 1999-06-30 2005-07-14 Advanced Cell Technology, Inc. Cytoplasmic transfer to de-differentiate recipient cells
US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
JP2003525031A (ja) * 1999-09-14 2003-08-26 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス 交雑種核移植によって産生された胚細胞または幹様細胞系及びドナー細胞の遺伝子改変または組織培養条件によって胚発達を増強する方法
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
AU2003211801A1 (en) * 2002-02-27 2003-09-09 Horri, Takuro Embryonically modified animal and method of constructing the same
AU2004238375A1 (en) * 2003-05-13 2004-11-25 The Regents Of The University Of Colorado Diagnostic and therapeutic treatments related to mitochondrial disorders
EP1984487B1 (fr) 2005-08-03 2022-10-12 Astellas Institute for Regenerative Medicine Procedes ameliores de reprogrammation de cellules somatiques animales
US8372644B2 (en) * 2006-02-06 2013-02-12 Transplantation Limited Method for growing adult cells
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy
EP4343331A3 (fr) 2015-03-23 2024-06-19 Astellas Institute for Regenerative Medicine Dosages améliorés pour la puissance de cellules épithéliales pigmentaires rétiniennes humaines (erp) et de progéniteurs de photorécepteurs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
AU740709B2 (en) * 1996-08-19 2001-11-15 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplanta tion
WO1999005266A2 (fr) * 1997-07-26 1999-02-04 Wisconsin Alumni Research Foundation Transfert de noyau entre des especes differentes
IL138201A0 (en) * 1998-03-02 2001-10-31 Univ Massachusetts Embryonic or stem-like cell lines produced by cross-species nuclear transplantation
GB9809178D0 (en) * 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
EP1109890A4 (fr) * 1999-06-30 2004-12-29 Hwang Woo Suk Procede d'obtention d'embryons humains clones au moyen de techniques de transplantation inter-especes de noyaux
JP2003525031A (ja) * 1999-09-14 2003-08-26 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス 交雑種核移植によって産生された胚細胞または幹様細胞系及びドナー細胞の遺伝子改変または組織培養条件によって胚発達を増強する方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9550974B2 (en) 2004-11-04 2017-01-24 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US9617512B2 (en) 2004-11-04 2017-04-11 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells and embryo-derived cells
US10072243B2 (en) 2004-11-04 2018-09-11 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells and embryo-derived cells
US10584313B2 (en) 2007-02-23 2020-03-10 Astellas Institute For Regenerative Medicine Method of producing a differentiated mammalian cell comprising culturing a single mammalian blastomere

Also Published As

Publication number Publication date
WO2001046401A8 (fr) 2001-07-26
WO2001046401A1 (fr) 2001-06-28
IL150065A (en) 2009-09-22
EP1240318A1 (fr) 2002-09-18
AU4830900A (en) 2001-07-03
JP2004500066A (ja) 2004-01-08
AU784731B2 (en) 2006-06-08
BR0016561A (pt) 2002-09-10
CN1425064A (zh) 2003-06-18
NZ519346A (en) 2004-02-27
MXPA02006093A (es) 2004-08-23
EP1240318A4 (fr) 2004-12-08
IL150065A0 (en) 2002-12-01

Similar Documents

Publication Publication Date Title
AU740709B2 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplanta tion
AU742363B2 (en) Nuclear transfer with differentiated fetal and adult donor cells
US20010012513A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation
CA2317494A1 (fr) Clonage faisant appel a des noyaux donnes de cellules foetales et adultes differenciees
WO2000052145A2 (fr) Lignees cellulaires embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees
NZ502129A (en) Cloning using donor nuclei from non-serum starved, differentiated cells
US7696404B2 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
AU784731B2 (en) Embryonic or stem-like cells produced by cross species nuclear transplantation
AU759322B2 (en) Embryonic or stem-like cell lines produced by cross-species nuclear transplantation
AU2008229989A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
CA2384413A1 (fr) Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees et technique destinee a renforcer le developpement embryonnaire par modification genetique de cellules donneuses ou par culture de tissus.
US20020194637A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation
AU2005225103A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
AU2006201208A1 (en) Embryonic or stem-like cells produced by cross species nuclear transpantation
MXPA00008602A (en) Embryonic or stem-like cell lines produced by cross-species nuclear transplantation

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued