CA2392777A1 - Methods of identifying compounds that bind to target species under isothermal denaturing conditions - Google Patents
Methods of identifying compounds that bind to target species under isothermal denaturing conditions Download PDFInfo
- Publication number
- CA2392777A1 CA2392777A1 CA002392777A CA2392777A CA2392777A1 CA 2392777 A1 CA2392777 A1 CA 2392777A1 CA 002392777 A CA002392777 A CA 002392777A CA 2392777 A CA2392777 A CA 2392777A CA 2392777 A1 CA2392777 A1 CA 2392777A1
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- Prior art keywords
- target species
- denaturation
- protein
- test
- test compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
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- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
- Y10T436/115831—Condition or time responsive
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
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- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/458,171 | 1999-12-09 | ||
| US09/458,171 US6376180B1 (en) | 1999-12-09 | 1999-12-09 | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
| PCT/US2000/042677 WO2001046693A2 (en) | 1999-12-09 | 2000-12-07 | Screening under isothermal denaturing conditions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2392777A1 true CA2392777A1 (en) | 2001-06-28 |
Family
ID=23819666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002392777A Abandoned CA2392777A1 (en) | 1999-12-09 | 2000-12-07 | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6376180B1 (https=) |
| EP (1) | EP1364213A2 (https=) |
| JP (1) | JP2004502130A (https=) |
| AU (1) | AU775000B2 (https=) |
| CA (1) | CA2392777A1 (https=) |
| TW (1) | TWI240073B (https=) |
| WO (1) | WO2001046693A2 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10509053A (ja) * | 1995-09-08 | 1998-09-08 | スクリプトジェン・ファーマスーティカルズ，インコーポレイテッド | Ｒｎａに対する親和性を有する化合物のためのスクリーン |
| PL194483B1 (pl) | 1997-11-12 | 2007-06-29 | Dimensional Pharmaceuticals 3 | Sposób wyznaczania funkcji biologicznej docelowego białka |
| US6569631B1 (en) * | 1998-11-12 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes |
| US6582907B1 (en) * | 1999-12-09 | 2003-06-24 | Pharmacia & Upjohn Company | Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions |
| AU2001247660A1 (en) * | 2000-03-21 | 2001-10-03 | The Board Of Trustees Of The University Of Illinois | Colorimetric artificial nose having an array of dyes and method for artificial olfaction |
| EP1409982A4 (en) * | 2001-06-14 | 2006-05-24 | Anadys Pharmaceuticals Inc | PROCESS FOR SCREENING ON LIGANDS OF TARGET MOLECULES |
| US7672786B2 (en) * | 2003-07-02 | 2010-03-02 | Sergey Krylov | Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development |
| JP2006010491A (ja) * | 2004-06-25 | 2006-01-12 | Mitsubishi Kagaku Bio-Clinical Laboratories Inc | 体液分析方法及び分注良否判定装置 |
| GB201106548D0 (en) | 2011-04-18 | 2011-06-01 | Evitraproteoma Ab | A method for determining ligand binding to a target protein using a thermal shift assahy |
| US9523693B2 (en) | 2011-04-18 | 2016-12-20 | Biotarget Engagement Interest Group Ab | Methods for determining ligand binding to a target protein using a thermal shift assay |
| WO2016028804A1 (en) | 2014-08-21 | 2016-02-25 | Discoverx Corporation | Homogenous thermal shift ligand binding assay |
| WO2016127100A1 (en) * | 2015-02-05 | 2016-08-11 | Promega Corporation | Luciferase-based thermal shift assays |
| CN109444209A (zh) * | 2018-10-26 | 2019-03-08 | 北京食品科学研究院 | 一种测量蛋清粉玻璃化转变温度的方法 |
| CN110095421B (zh) * | 2019-05-17 | 2022-02-08 | 淮海工学院 | 一种胶原蛋白变性温度的测定方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3939350A (en) * | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US5585277A (en) | 1993-06-21 | 1996-12-17 | Scriptgen Pharmaceuticals, Inc. | Screening method for identifying ligands for target proteins |
| US5679582A (en) | 1993-06-21 | 1997-10-21 | Scriptgen Pharmaceuticals, Inc. | Screening method for identifying ligands for target proteins |
| CA2184195C (en) * | 1995-10-25 | 2002-04-16 | Andrew Pakula | Screening method for identifying ligands for target proteins |
| JP2000502440A (ja) * | 1995-12-07 | 2000-02-29 | スクリプトジェン・ファーマスーティカルズ，インコーポレイテッド | リガンドを識別するための蛍光に基づくスクリーニング方法 |
| AU741049B2 (en) | 1996-05-09 | 2001-11-22 | Life Technologies Corporation | Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization |
| EP0836090A1 (en) | 1996-10-12 | 1998-04-15 | Evotec BioSystems GmbH | Method of analysis of samples by determination of the distribution of specific brightnesses of particles |
| DE69811724T2 (de) * | 1997-12-05 | 2003-10-16 | Pharmacia & Upjohn Co., Kalamazoo | Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase |
| US6242190B1 (en) * | 1999-12-01 | 2001-06-05 | John Hopkins University | Method for high throughput thermodynamic screening of ligands |
-
1999
- 1999-12-09 US US09/458,171 patent/US6376180B1/en not_active Expired - Fee Related
-
2000
- 2000-12-04 TW TW089125804A patent/TWI240073B/zh not_active IP Right Cessation
- 2000-12-07 CA CA002392777A patent/CA2392777A1/en not_active Abandoned
- 2000-12-07 AU AU47137/01A patent/AU775000B2/en not_active Ceased
- 2000-12-07 EP EP00992870A patent/EP1364213A2/en not_active Withdrawn
- 2000-12-07 WO PCT/US2000/042677 patent/WO2001046693A2/en not_active Ceased
- 2000-12-07 JP JP2001547549A patent/JP2004502130A/ja active Pending
-
2001
- 2001-11-13 US US10/008,090 patent/US6713260B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004502130A (ja) | 2004-01-22 |
| AU4713701A (en) | 2001-07-03 |
| US20020068298A1 (en) | 2002-06-06 |
| US6376180B1 (en) | 2002-04-23 |
| US6713260B2 (en) | 2004-03-30 |
| EP1364213A2 (en) | 2003-11-26 |
| TWI240073B (en) | 2005-09-21 |
| AU775000B2 (en) | 2004-07-15 |
| WO2001046693A2 (en) | 2001-06-28 |
| WO2001046693A3 (en) | 2003-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |