CA2385823A1 - Production de virus associes a l'adenovirus (aav) recombinants mettant en oeuvre un adenovirus comprenant des genes rep/cap associes a l'adenovirus - Google Patents
Production de virus associes a l'adenovirus (aav) recombinants mettant en oeuvre un adenovirus comprenant des genes rep/cap associes a l'adenovirus Download PDFInfo
- Publication number
- CA2385823A1 CA2385823A1 CA002385823A CA2385823A CA2385823A1 CA 2385823 A1 CA2385823 A1 CA 2385823A1 CA 002385823 A CA002385823 A CA 002385823A CA 2385823 A CA2385823 A CA 2385823A CA 2385823 A1 CA2385823 A1 CA 2385823A1
- Authority
- CA
- Canada
- Prior art keywords
- raav
- promoter
- aav
- gene
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Abstract
La présente invention concerne de nouveaux adénovirus utiles dans la production de titres élevés de virus associés à l'adénovirus recombinants (AAVr) comprenant un insert d'ADN étranger et des procédés de fabrication desdits adénovirus. Les adénovirus comportent le gène AAV rep dans lequel le promoteur p5 de rep est substitué par un promoteur minimal ou par aucun promoteur. L'invention concerne également des procédés de production de AAVr de haut niveau sous forme d'une préparation sensiblement homogène et des compositions de AAVr.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15746699P | 1999-10-01 | 1999-10-01 | |
US60/157,466 | 1999-10-01 | ||
PCT/US2000/026948 WO2001025462A1 (fr) | 1999-10-01 | 2000-09-29 | Production de virus associes a l'adenovirus (aav) recombinants mettant en oeuvre un adenovirus comprenant des genes rep/cap associes a l'adenovirus |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2385823A1 true CA2385823A1 (fr) | 2001-04-12 |
Family
ID=22563855
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002385823A Abandoned CA2385823A1 (fr) | 1999-10-01 | 2000-09-29 | Production de virus associes a l'adenovirus (aav) recombinants mettant en oeuvre un adenovirus comprenant des genes rep/cap associes a l'adenovirus |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1222299A1 (fr) |
JP (1) | JP2003511037A (fr) |
AU (1) | AU7841400A (fr) |
CA (1) | CA2385823A1 (fr) |
WO (1) | WO2001025462A1 (fr) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10045687B4 (de) * | 2000-09-15 | 2006-07-06 | MICROMUN Privates Institut für Mikrobiologische Forschung GmbH Biotechnikum Greifswald | Expressionskassetten und Adenovirusvektoren |
EP3517134B1 (fr) | 2001-12-17 | 2024-01-17 | The Trustees of the University of Pennsylvania | Séquences de virus adéno-associés de sérotype 8 , vecteurs les contenant et leurs utilisations |
US8043856B2 (en) | 2007-06-14 | 2011-10-25 | Wisconsin Alumni Research Foundation | Adenoviral vectors for influenza virus production |
CA2689588C (fr) * | 2007-06-21 | 2016-08-23 | Alphavax, Inc. | Cassettes sans promoteur pour l'expression de proteines structurales d'alphavirus |
EP2368903B1 (fr) * | 2010-03-26 | 2014-03-05 | Deutsches Krebsforschungszentrum | Virus transporteur dérivé d'adénovirus pour améliorer la production de parvovirus recombinant |
RU2705249C2 (ru) * | 2013-07-12 | 2019-11-06 | Дзе Чилдрен'З Хоспитал Оф Филадельфия | Вектор aav и анализ на нейтрализующие антитела против aav (аденоассоциированного вируса) |
KR102390075B1 (ko) | 2014-03-09 | 2022-04-26 | 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 | 오르니틴 트랜스카르바밀라아제(otc) 결핍증의 치료에 유용한 조성물 |
US10053671B2 (en) | 2014-06-20 | 2018-08-21 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
HUE046643T2 (hu) * | 2014-11-28 | 2021-11-29 | Uniqure Ip Bv | DNS szennyezõdések parvovirális virionokat tartalmazó készítményben |
IL296391B1 (en) * | 2015-01-20 | 2024-02-01 | Genzyme Corp | Analytical ultracentrifugation for characterization of recombinant viral particles |
WO2020163804A1 (fr) | 2019-02-08 | 2020-08-13 | Wisconsin Alumni Research Foundation (Warf) | Lignée cellulaire humanisée |
US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5173414A (en) * | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
US5856152A (en) * | 1994-10-28 | 1999-01-05 | The Trustees Of The University Of Pennsylvania | Hybrid adenovirus-AAV vector and methods of use therefor |
EP0922110B1 (fr) * | 1996-08-15 | 2008-04-16 | The Government of the United States of America, represented by the Secretary, Department of Health and Human Services | Regulation spatiale et temporelle de l'expression genique au moyen d'un promoteur proteique du stress en combination avec une source de chaleur locale |
US6548286B1 (en) * | 1997-04-14 | 2003-04-15 | Cell Genesys, Inc. | Methods for increasing the efficiency of recombinant AAV product |
-
2000
- 2000-09-29 AU AU78414/00A patent/AU7841400A/en not_active Abandoned
- 2000-09-29 WO PCT/US2000/026948 patent/WO2001025462A1/fr not_active Application Discontinuation
- 2000-09-29 JP JP2001528613A patent/JP2003511037A/ja not_active Withdrawn
- 2000-09-29 CA CA002385823A patent/CA2385823A1/fr not_active Abandoned
- 2000-09-29 EP EP00968512A patent/EP1222299A1/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
AU7841400A (en) | 2001-05-10 |
JP2003511037A (ja) | 2003-03-25 |
EP1222299A1 (fr) | 2002-07-17 |
WO2001025462A1 (fr) | 2001-04-12 |
WO2001025462A9 (fr) | 2002-11-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |