CA2382609C - Expression de proteines dans des champignons modifies par genie genetique - Google Patents

Expression de proteines dans des champignons modifies par genie genetique Download PDF

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Publication number
CA2382609C
CA2382609C CA2382609A CA2382609A CA2382609C CA 2382609 C CA2382609 C CA 2382609C CA 2382609 A CA2382609 A CA 2382609A CA 2382609 A CA2382609 A CA 2382609A CA 2382609 C CA2382609 C CA 2382609C
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CA
Canada
Prior art keywords
gene
fungal cell
filamentous fungal
xylanase
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2382609A
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English (en)
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CA2382609A1 (fr
Inventor
Sylvia Mchugh
Christopher D. Hindle
Theresa C. White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iogen Corp
Original Assignee
Iogen Bio Products Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/392,476 external-priority patent/US6939704B1/en
Application filed by Iogen Bio Products Corp filed Critical Iogen Bio Products Corp
Publication of CA2382609A1 publication Critical patent/CA2382609A1/fr
Application granted granted Critical
Publication of CA2382609C publication Critical patent/CA2382609C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

Cette invention a trait au renforcement de la production d'une protéine étudiée issue d'un hôte fongique. Elle porte sur des séquences nucléotidiques comportant une région régulatrice associée fonctionnellement à une séquence de sécrétion de xylanase et à un gène étudié. Ce gène, étudié dans le cadre de l'invention, code une protéine sélectionnée dans le groupe constitué par des substances pharmaceutiques, des aliments fonctionnels, des substances industrielles, des produits d'alimentation animale, des additifs alimentaires et une enzyme. Ce gène code, de préférence, une cellulase, une hémicellulase, une enzyme dégradant la lignine, une pectinase, une protéase ou une peroxydase. L'invention concerne également des vecteurs et des hôtes comportant ces séquences nucléotidiques ainsi que des procédés de production de la protéine susmentionnée.
CA2382609A 1999-09-09 2000-09-01 Expression de proteines dans des champignons modifies par genie genetique Expired - Fee Related CA2382609C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/392,476 1999-09-09
US09/392,476 US6939704B1 (en) 1998-03-10 1999-09-09 Enhanced expression of proteins in genetically modified fungi
PCT/CA2000/000997 WO2001018218A1 (fr) 1999-09-09 2000-09-01 Expression de proteines dans des champignons modifies par genie genetique

Publications (2)

Publication Number Publication Date
CA2382609A1 CA2382609A1 (fr) 2001-03-15
CA2382609C true CA2382609C (fr) 2012-08-07

Family

ID=23550748

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2382609A Expired - Fee Related CA2382609C (fr) 1999-09-09 2000-09-01 Expression de proteines dans des champignons modifies par genie genetique

Country Status (7)

Country Link
EP (1) EP1214431A1 (fr)
CN (1) CN1390260A (fr)
AU (1) AU6974400A (fr)
BR (1) BR0013896A (fr)
CA (1) CA2382609C (fr)
MX (1) MX250271B (fr)
WO (1) WO2001018218A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2298904B1 (fr) 2002-06-14 2017-05-24 BP Corporation North America Inc. Xylanases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation
USRE45660E1 (en) 2006-02-14 2015-09-01 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
US8198046B2 (en) 2006-07-11 2012-06-12 Danisco Us Inc. KEX2 cleavage regions of recombinant fusion proteins
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
WO2010059424A2 (fr) * 2008-11-18 2010-05-27 Novozymes, Inc. Procédés et compositions pour dégrader un matériau cellulosique
CN111690629B (zh) * 2020-05-29 2022-04-19 浙江工业大学 一种内切葡聚糖苷酶突变体、基因、工程菌及其应用
CN114426987A (zh) * 2022-01-14 2022-05-03 湖南省蔬菜研究所 一种辣椒白绢病菌的遗传转化方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993000426A1 (fr) * 1991-06-25 1993-01-07 Novo Nordisk A/S Lipase pancreatique de mammifere et sa variante
US5591619A (en) * 1994-09-30 1997-01-07 University Of Georgia Research Foundation, Inc. Aureobasidium pullulans xylanase, gene and signal sequence
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
EP1214431A1 (fr) 2002-06-19
CN1390260A (zh) 2003-01-08
WO2001018218A9 (fr) 2002-11-07
MX250271B (es) 2007-10-12
WO2001018218B1 (fr) 2001-04-19
WO2001018218A1 (fr) 2001-03-15
MXPA02002516A (es) 2005-11-17
BR0013896A (pt) 2002-05-14
CA2382609A1 (fr) 2001-03-15
AU6974400A (en) 2001-04-10

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