CA2381139A1 - Procede pour produire une proteine formant un canal - Google Patents

Procede pour produire une proteine formant un canal Download PDF

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Publication number
CA2381139A1
CA2381139A1 CA002381139A CA2381139A CA2381139A1 CA 2381139 A1 CA2381139 A1 CA 2381139A1 CA 002381139 A CA002381139 A CA 002381139A CA 2381139 A CA2381139 A CA 2381139A CA 2381139 A1 CA2381139 A1 CA 2381139A1
Authority
CA
Canada
Prior art keywords
gene
channel
mspa
aforementioned
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002381139A
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English (en)
Inventor
Michael Niederweis
Stefan Bossmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19943520A external-priority patent/DE19943520A1/de
Application filed by Individual filed Critical Individual
Publication of CA2381139A1 publication Critical patent/CA2381139A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé pour produire une protéine formant un canal, présente dans des bactéries à Gram positif. Selon ce procédé, cette protéine est obtenue par a) surexpression hétérologue ou b) purification de la protéine contenue dans des mycobactéries, la température d'extraction étant supérieure à 50 ·C.
CA002381139A 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal Abandoned CA2381139A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE19941416.5 1999-08-31
DE19941416 1999-08-31
DE19943520.0 1999-09-11
DE19943520A DE19943520A1 (de) 1999-08-31 1999-09-11 Verfahren zur Herstellung eines kanalbildenden Proteins
PCT/DE2000/002924 WO2001016327A2 (fr) 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal

Publications (1)

Publication Number Publication Date
CA2381139A1 true CA2381139A1 (fr) 2001-03-08

Family

ID=26054779

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002381139A Abandoned CA2381139A1 (fr) 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal

Country Status (6)

Country Link
EP (1) EP1208207A2 (fr)
JP (1) JP2003508054A (fr)
AU (1) AU7901300A (fr)
CA (1) CA2381139A1 (fr)
DE (1) DE10082576D2 (fr)
WO (1) WO2001016327A2 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10472673B2 (en) 2015-02-19 2019-11-12 Oxford Nanopore Technologies Ltd. Hetero-pores
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US11307192B2 (en) 2015-02-19 2022-04-19 Oxford Nanopore Technologies Plc Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores
US12024541B2 (en) 2017-05-04 2024-07-02 Oxford Nanopore Technologies Plc Transmembrane pore consisting of two CsgG pores

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202009019021U1 (de) 2008-09-22 2015-09-24 University Of Washington Msp-nanoporen
DK3131920T3 (da) 2014-04-16 2021-11-22 Uab Res Found Msp-nanoporer og anvendelser deraf

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU682538B2 (en) * 1993-11-16 1997-10-09 Becton Dickinson & Company Process for lysing mycobacteria
JP2002514385A (ja) * 1997-12-23 2002-05-21 ジェネシス リサーチ アンド デベロップメント コーポレイション リミテッド ミコバクテリウム・バッケー由来の組成物およびその使用法

Cited By (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US11845780B2 (en) 2012-04-10 2023-12-19 Oxford Nanopore Technologies Plc Mutant lysenin pores
US10882889B2 (en) 2012-04-10 2021-01-05 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US10514378B2 (en) 2013-03-25 2019-12-24 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US11761956B2 (en) 2013-03-25 2023-09-19 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10976311B2 (en) 2013-03-25 2021-04-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10802015B2 (en) 2013-03-25 2020-10-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US11725235B2 (en) 2014-01-22 2023-08-15 Oxford Nanopore Technologies Plc Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10844432B2 (en) 2014-05-02 2020-11-24 Oxford Nanopore Technologies, Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
US11739377B2 (en) 2014-05-02 2023-08-29 Oxford Nanopore Technologies Plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US11034734B2 (en) 2014-09-01 2021-06-15 Oxford Nanopore Technologies Ltd. Mutant pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
US10472673B2 (en) 2015-02-19 2019-11-12 Oxford Nanopore Technologies Ltd. Hetero-pores
US11307192B2 (en) 2015-02-19 2022-04-19 Oxford Nanopore Technologies Plc Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
US10995372B2 (en) 2016-03-02 2021-05-04 Oxford Nanopore Technologies Ltd. Mutant pores
US11597970B2 (en) 2016-03-02 2023-03-07 Oxford Nanopore Technologies Plc Mutant pores
US11685949B2 (en) 2016-03-02 2023-06-27 Oxford Nanopore Technologies Plc Mutant pore
US11186868B2 (en) 2016-03-02 2021-11-30 Oxford Nanopore Technologies Plc Mutant pore
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
US12018326B2 (en) 2016-03-02 2024-06-25 Oxford Nanopore Technologies Plc Mutant pore
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
US11939359B2 (en) 2016-04-06 2024-03-26 Oxford Nanopore Technologies Plc Mutant pore
US12024541B2 (en) 2017-05-04 2024-07-02 Oxford Nanopore Technologies Plc Transmembrane pore consisting of two CsgG pores
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores
US11945840B2 (en) 2017-06-30 2024-04-02 Vib Vzw Protein pores

Also Published As

Publication number Publication date
EP1208207A2 (fr) 2002-05-29
DE10082576D2 (de) 2002-12-19
AU7901300A (en) 2001-03-26
WO2001016327A3 (fr) 2001-05-31
JP2003508054A (ja) 2003-03-04
WO2001016327A2 (fr) 2001-03-08

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Legal Events

Date Code Title Description
FZDE Discontinued