CA2379749A1 - Deplacement de brins multiplexes pour determinations d'acide nucleique - Google Patents

Deplacement de brins multiplexes pour determinations d'acide nucleique Download PDF

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Publication number
CA2379749A1
CA2379749A1 CA002379749A CA2379749A CA2379749A1 CA 2379749 A1 CA2379749 A1 CA 2379749A1 CA 002379749 A CA002379749 A CA 002379749A CA 2379749 A CA2379749 A CA 2379749A CA 2379749 A1 CA2379749 A1 CA 2379749A1
Authority
CA
Canada
Prior art keywords
primer
capture
oligonucleotides
sequences
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379749A
Other languages
English (en)
Inventor
Sharat Singh
Anita Inamdar
Edwin F. Ullman
Liching Cao
David Albagli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monogram Biosciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379749A1 publication Critical patent/CA2379749A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des déterminations multiplexées de grands nombres d'événements sont obtenues d'une manière précise et simple à l'aide d'une combinaison de différents oligonucléotides, lesquels servent à la fois à la capture et à la libération sur et à partir d'un support, et des oligonucléotides de déplacement de brins destinés à la libération séquentielle de groupes d'oligonucléotides. Sont également compris, faisant partie des réactifs oligonucléotidiques, des identificateurs, lesquels servent à identifier une caractéristique particulière. La méthode est illustrée à l'aide d'amorces pour l'amorçage de prolongement de chaîne joints à ou servant de séquence de capture/libération, l'amorce prolongée ayant un identificateur. Après prolongement de l'amorce, les amorces prolongées sont capturées et libérées indépendamment et les amorces prolongées libérées sont dosées. La méthode de l'invention trouve une application dans des déterminations de séquences d'acide nucléique, des déterminations de polymorphismes mononucléotidiques, l'identification de fragments d'acide nucléique et analogue.
CA002379749A 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique Abandoned CA2379749A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35462999A 1999-07-16 1999-07-16
US09/354,629 1999-07-16
PCT/US2000/018301 WO2001006008A2 (fr) 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique

Publications (1)

Publication Number Publication Date
CA2379749A1 true CA2379749A1 (fr) 2001-01-25

Family

ID=23394232

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379749A Abandoned CA2379749A1 (fr) 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique

Country Status (4)

Country Link
EP (1) EP1200624A2 (fr)
JP (1) JP2003505036A (fr)
CA (1) CA2379749A1 (fr)
WO (1) WO2001006008A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029109A2 (fr) * 2000-10-05 2002-04-11 Aclara Biosciences, Inc. Deplacement differentiel multiplexe pour determinations d'acides nucleiques
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
DE602005018316D1 (de) * 2004-10-08 2010-01-28 Sysmex Corp Verfahren zum Nachweis einer Zielsubstanz unter Verwendung einer Nukleinsäuresonde
WO2024123917A1 (fr) * 2022-12-07 2024-06-13 Illumina, Inc. Regroupement monoclonal par exclusion stérique d'adn double brin avec ensemencement à motifs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0286642A4 (fr) * 1985-12-17 1990-06-27 Genetics Inst Procede d'analyse de polynucleotides base sur le deplacement des brins, et complexe reactif.
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
DE3681176D1 (de) * 1986-12-01 1991-10-02 Molecular Biosystems Inc Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
GB9517955D0 (en) * 1995-07-25 1995-11-08 Univ Strathclyde Nucleotide sequence detection and analysis
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
JP2002505846A (ja) * 1997-11-06 2002-02-26 モザイク テクノロジーズ シグナル増幅およびプロセッシングのための多重逐次的ポリヌクレオチド置換反応

Also Published As

Publication number Publication date
EP1200624A2 (fr) 2002-05-02
WO2001006008A3 (fr) 2001-07-12
WO2001006008A2 (fr) 2001-01-25
JP2003505036A (ja) 2003-02-12

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Legal Events

Date Code Title Description
FZDE Discontinued