CA2379693A1 - Amplification d'acides nucleiques avec detection electronique - Google Patents

Amplification d'acides nucleiques avec detection electronique Download PDF

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Publication number
CA2379693A1
CA2379693A1 CA002379693A CA2379693A CA2379693A1 CA 2379693 A1 CA2379693 A1 CA 2379693A1 CA 002379693 A CA002379693 A CA 002379693A CA 2379693 A CA2379693 A CA 2379693A CA 2379693 A1 CA2379693 A1 CA 2379693A1
Authority
CA
Canada
Prior art keywords
probe
sequence
probes
preferred
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379693A
Other languages
English (en)
Inventor
Gary Blackburn
Bruce D. Irvine
Jon Faiz Kayyem
Edward Lewis Sheldon Iii
Robert H. Terbrueggen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Clinical Micro Sensors Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379693A1 publication Critical patent/CA2379693A1/fr
Abandoned legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des compositions et des procédés utiles dans la détection d'acides nucléiques au moyen de diverses techniques d'amplification, y compris l'amplification de signal et l'amplification de cible. La détection est mise en oeuvre à l'aide d'une fraction de transfert d'électrons (ETM) qui est associée à l'acide nucléique, directement ou indirectement, pour permettre une détection électronique de ladite ETM par une électrode.
CA002379693A 1999-07-20 2000-07-20 Amplification d'acides nucleiques avec detection electronique Abandoned CA2379693A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14469899P 1999-07-20 1999-07-20
US60/144,698 1999-07-20
PCT/US2000/019889 WO2001006016A2 (fr) 1999-07-20 2000-07-20 Amplification d'acides nucleiques avec detection electronique

Publications (1)

Publication Number Publication Date
CA2379693A1 true CA2379693A1 (fr) 2001-01-25

Family

ID=22509738

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379693A Abandoned CA2379693A1 (fr) 1999-07-20 2000-07-20 Amplification d'acides nucleiques avec detection electronique

Country Status (5)

Country Link
EP (1) EP1194593A2 (fr)
JP (1) JP2003530822A (fr)
AU (1) AU6360500A (fr)
CA (1) CA2379693A1 (fr)
WO (1) WO2001006016A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1241000A (en) 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7341837B2 (en) * 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
EP1907586A2 (fr) * 2005-07-15 2008-04-09 Applera Corporation Détection d'amplification d'acides nucléiques
EP2069525A1 (fr) * 2006-12-29 2009-06-17 Applied Biosystems, LLC Systèmes et procédés de détection d'acides nucléiques
EP2118310B1 (fr) * 2006-12-29 2013-03-06 Applied Biosystems, LLC Systemes et procedes pour la detection d'acide nucleique
JP2009034052A (ja) * 2007-08-02 2009-02-19 Canon Inc ハイブリダイゼーション方法および装置
WO2009029868A1 (fr) * 2007-08-31 2009-03-05 University Of Massachusetts Analogues nucléosidiques de phosphoramidite
CA2700945A1 (fr) * 2007-09-27 2009-04-02 University Of Western Ontario Nucleotide triphosphate avec marqueur electroactif conjugue au phosphate gamma
WO2009061941A2 (fr) * 2007-11-06 2009-05-14 Osmetech Molecular Diagnostics Analogues de nucléotide sans base et leurs utilisations
US20210164016A1 (en) * 2017-08-04 2021-06-03 Stratos Genomics Inc. Single molecule nucleic acid detection by mismatch cleavage
US20190062809A1 (en) 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
US20210292819A1 (en) * 2018-12-13 2021-09-23 Hewlett-Packard Development Company, L.P. Multiplex nucleic acid detection
CN114113265B (zh) * 2021-12-14 2023-08-08 郑州轻工业大学 一种适配体传感器及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8507706D0 (en) * 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
EP0939762A2 (fr) * 1996-11-05 1999-09-08 Clinical Micro Sensors Electrodes reliees par l'intermediaire d'oligomeres conducteurs a des acides nucleiques
US7560237B2 (en) * 1997-06-12 2009-07-14 Osmetech Technology Inc. Electronics method for the detection of analytes
AU764926B2 (en) * 1998-01-27 2003-09-04 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection

Also Published As

Publication number Publication date
WO2001006016A9 (fr) 2002-07-11
WO2001006016A2 (fr) 2001-01-25
WO2001006016A3 (fr) 2001-08-23
AU6360500A (en) 2001-02-05
JP2003530822A (ja) 2003-10-21
EP1194593A2 (fr) 2002-04-10

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Legal Events

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EEER Examination request
FZDE Dead