CA2377669A1 - Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels - Google Patents

Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels Download PDF

Info

Publication number
CA2377669A1
CA2377669A1 CA002377669A CA2377669A CA2377669A1 CA 2377669 A1 CA2377669 A1 CA 2377669A1 CA 002377669 A CA002377669 A CA 002377669A CA 2377669 A CA2377669 A CA 2377669A CA 2377669 A1 CA2377669 A1 CA 2377669A1
Authority
CA
Canada
Prior art keywords
polypeptide
dioxygenase
improved
recombinant
substituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002377669A
Other languages
English (en)
Inventor
Lisa M. Newman
Sergey A. Selifonov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2377669A1 publication Critical patent/CA2377669A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des polypeptides améliorés, dont les dioxygénases, les déshydrogénases, les ligases et les transférases, qui sont utiles pour la synthèse biocatalytique de composés, tels que les acides .alpha.-hydroxycarboxyliques, et de composés d'acide aryl- et alkyl-hydroxy et carboxyliques. Lesdits polypeptides présentent des propriétés améliorées, telles que la régiosélectivité, l'activité enzymatique, la stéréospécificité et similaire. L'invention porte également sur des méthodes de production de polynucléotides recombinés qui codent pour lesdits polypeptides améliorés, qui sont des organismes exprimant le polypeptide et sont donc utiles pour la mise en oeuvre desdites synthèses biocatalytiques. Des méthodes d'augmentation de la résistance aux solvants des organismes utilisés dans les méthodes synthétiques sont également décrites.
CA002377669A 1999-08-12 2000-08-11 Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels Abandoned CA2377669A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14885099P 1999-08-12 1999-08-12
US60/148,850 1999-08-12
PCT/US2000/022038 WO2001012791A1 (fr) 1999-08-12 2000-08-11 Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels

Publications (1)

Publication Number Publication Date
CA2377669A1 true CA2377669A1 (fr) 2001-02-22

Family

ID=22527694

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002377669A Abandoned CA2377669A1 (fr) 1999-08-12 2000-08-11 Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels

Country Status (6)

Country Link
EP (1) EP1208193A1 (fr)
AU (1) AU6634300A (fr)
CA (1) CA2377669A1 (fr)
IL (1) IL147384A0 (fr)
MX (1) MXPA01013201A (fr)
WO (1) WO2001012791A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114561432A (zh) * 2020-11-27 2022-05-31 中国科学院天津工业生物技术研究所 一种芳香族化合物的开环方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001271280A1 (en) * 2000-05-31 2001-12-11 Maxygen, Inc. Preparation of 4-hydroxy-3(2h)-furanones
EP1646712A4 (fr) * 2003-04-14 2008-10-22 Du Pont Procede de preparation de para-hydroxystyrene par decarboxylation biocatalytique d'acide para-hydroxycinnamique dans un milieu de reaction a deux phases
DE10350474C5 (de) 2003-10-23 2009-09-17 C-Lecta Gmbh Verfahren zur Selektion von Biomolekülen aus Varianten-Bibliotheken von Biomolekülen
DE102004038154A1 (de) * 2004-08-06 2006-03-16 Maxens Gmbh Technologisch hergestelltes Dihydrocoumarin
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
WO2018005655A2 (fr) * 2016-06-30 2018-01-04 Zymergen Inc. Procédés de génération d'une bibliothèque d'hémoglobines bactériennes et utilisations associées
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114561432A (zh) * 2020-11-27 2022-05-31 中国科学院天津工业生物技术研究所 一种芳香族化合物的开环方法

Also Published As

Publication number Publication date
EP1208193A1 (fr) 2002-05-29
IL147384A0 (en) 2002-08-14
MXPA01013201A (es) 2002-06-04
AU6634300A (en) 2001-03-13
WO2001012791A1 (fr) 2001-02-22

Similar Documents

Publication Publication Date Title
US6605430B1 (en) DNA shuffling of monooxygenase genes for production of industrial chemicals
US7183089B2 (en) Method for enhancing production of isoprenoid compounds
Wong et al. Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds
US7723498B2 (en) Directed evolution of recombinant monooxygenase nucleic acids and related polypeptides and methods of use
Gao et al. Efficient biosynthesis of (2 S)-eriodictyol from (2 S)-naringenin in Saccharomyces cerevisiae through a combination of promoter adjustment and directed evolution
JP2001197895A (ja) 細胞工学および代謝工学のための方法および組成物
WO2001042455A1 (fr) Evolution dirigee de voies de biosynthese et de biodegradation
JP2002526107A (ja) マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
US20020072097A1 (en) Molecular breeding of transposable elements
CA2377669A1 (fr) Methode du dna shuffling appliquee aux genes de dioxygenase, pour la production de produits chimiques industriels
Groeneveld et al. Identification of a novel oxygenase capable of regiospecific hydroxylation of D-limonene into (+)-trans-carveol
Zielinski et al. Generation of novel-substrate-accepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity
US9353390B2 (en) Genetically engineered microbes and methods for producing 4-hydroxycoumarin
Hua et al. Offloading role of a discrete thioesterase in type II polyketide biosynthesis
Zhou et al. Versatile CYP98A enzymes catalyse meta‐hydroxylation reveals diversity of salvianolic acids biosynthesis
WO2001068803A2 (fr) Enzymes, voies et organismes pour la fabrication d'un monomere polymerisable par un bioprocessus cellulaire integral
CN115151643A (zh) 用于产生橄榄醇酸和橄榄醇酸类似物的生物合成平台
Bornscheuer et al. Evolving Enzymes for Biocatalysis 15
McLachlan et al. Directed enzyme evolution and high‐throughput screening
CN1378598A (zh) 酶的演化和对组合和医药化学的用途
WO2019052907A2 (fr) Procédé de détection de l'activité de conversion de peroxyde d'hydrogène d'une enzyme
Dennig Engineering of cytochrome P450 monooxygenases for application in phenol synthesis
Donoso et al. Identification of a self-sufficient cytochrome P450
Schmidt et al. Directed evolution of industrial biocatalysts
Kukor et al. Metabolism of aromatic compounds

Legal Events

Date Code Title Description
FZDE Discontinued