CA2375746A1 - Colonne amelioree pour la separation de l'adn en chromatographie de nucleotides a ions apparies (mipc) - Google Patents

Colonne amelioree pour la separation de l'adn en chromatographie de nucleotides a ions apparies (mipc) Download PDF

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Publication number
CA2375746A1
CA2375746A1 CA002375746A CA2375746A CA2375746A1 CA 2375746 A1 CA2375746 A1 CA 2375746A1 CA 002375746 A CA002375746 A CA 002375746A CA 2375746 A CA2375746 A CA 2375746A CA 2375746 A1 CA2375746 A1 CA 2375746A1
Authority
CA
Canada
Prior art keywords
column
beads
separation
dna
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002375746A
Other languages
English (en)
Inventor
Karl H. Hecker
Raquel Roque
Robert M. Haefele
Douglas T. Gjerde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/501,775 external-priority patent/US6372142B1/en
Application filed by Individual filed Critical Individual
Publication of CA2375746A1 publication Critical patent/CA2375746A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/366—Ion-pair, e.g. ion-pair reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G01N2030/3084—Control of physical parameters of the fluid carrier of temperature ovens

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

L'invention concerne une colonne de séparation améliorée et un procédé correspondant pour séparer un mélange de fragments d'ADN à double brin par chromatographie MIPC. La colonne cylindrique, qui a un diamètre interne supérieur à environ 5 mm, contient des perles de polymère dont le diamètre moyen est compris entre 1 et 100 microns. Il s'agit de perles non substituées ou substituées par une fraction hydrocarbure qui présente entre 1 atome de carbone et un million d'atomes de carbone. De préférence, ces perles sont sensiblement dépourvues de cations multivalents qui peuvent se lier librement avec l'ADN. La colonne améliorée assure une meilleure séparation des fragments d'ADN ayant une taille comprise entre environ 100 et 20 000 paires de base. En outre, cette colonne assure une séparation améliorée pour les molécules d'ADN hétéroduplex et homoduplex dans une procédure de détection de mutation où la chromatographie est accomplie selon des conditions de dénaturation partielle de l'ADN en un site de paires de base présentant un défaut d'appariement.
CA002375746A 1999-09-17 2000-09-18 Colonne amelioree pour la separation de l'adn en chromatographie de nucleotides a ions apparies (mipc) Abandoned CA2375746A1 (fr)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US15461499P 1999-09-17 1999-09-17
US60/154,614 1999-09-17
US15874399P 1999-10-12 1999-10-12
US60/158,743 1999-10-12
US16751599P 1999-11-24 1999-11-24
US60/167,515 1999-11-24
US17717700P 2000-01-20 2000-01-20
US60/177,177 2000-01-20
US09/501,775 US6372142B1 (en) 1996-11-13 2000-02-10 Column for DNA separation by matched ion polynucleotide chromatography
US09/501,775 2000-02-10
PCT/US2000/025532 WO2001019485A1 (fr) 1999-09-17 2000-09-18 Colonne amelioree pour la separation de l'adn en chromatographie de nucleotides a ions apparies (mipc)

Publications (1)

Publication Number Publication Date
CA2375746A1 true CA2375746A1 (fr) 2001-03-22

Family

ID=27538481

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002375746A Abandoned CA2375746A1 (fr) 1999-09-17 2000-09-18 Colonne amelioree pour la separation de l'adn en chromatographie de nucleotides a ions apparies (mipc)

Country Status (5)

Country Link
EP (1) EP1231993A4 (fr)
JP (1) JP2003509662A (fr)
AU (1) AU7384100A (fr)
CA (1) CA2375746A1 (fr)
WO (1) WO2001019485A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1278584B1 (fr) * 2000-04-21 2006-11-15 TRANSGENOMIC, Inc. Procede de lavage de colonne par chromatographie de polynucleotides a ions apparies
US7135289B2 (en) 2001-10-05 2006-11-14 Transgenomic, Inc. Methods and compositions for mutation analysis of polynucleotides by liquid chromatography
EP1565261A1 (fr) * 2002-11-25 2005-08-24 Varian, Inc. Particules copolymeres macroporeuses de forme irreguliere et leurs procedes d'utilisation
JP5670544B2 (ja) * 2013-11-27 2015-02-18 第一三共株式会社 オンライン不純物除去装置及び方法
RU2563277C1 (ru) * 2014-04-07 2015-09-20 Валерий Геннадьевич Брусов Способ автоматического управления узлом разгонки отходов брагоректификационной установки
GB201610792D0 (en) * 2016-06-21 2016-08-03 Ge Healthcare Bioprocess R&D Ab Method for packing chromatography columns

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
JP2000516638A (ja) * 1997-06-10 2000-12-12 トランスジエノミツク・インコーポレーテツド 液体クロマトグラフィーを使用するポリヌクレオチド分離を実施するための系および方法
JP2001512702A (ja) * 1997-08-05 2001-08-28 トランスジエノミツク・インコーポレーテツド 突然変異を検出するための変性マルチイオンポリヌクレオチドクロマトグラフィー

Also Published As

Publication number Publication date
EP1231993A4 (fr) 2003-04-16
JP2003509662A (ja) 2003-03-11
EP1231993A1 (fr) 2002-08-21
AU7384100A (en) 2001-04-17
WO2001019485A1 (fr) 2001-03-22

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Legal Events

Date Code Title Description
FZDE Discontinued