CA2374505A1 - Methods for display of heterodimeric proteins on filamentous phage using pvii and pix, compositions, vectors and combinatorial libraries - Google Patents

Methods for display of heterodimeric proteins on filamentous phage using pvii and pix, compositions, vectors and combinatorial libraries Download PDF

Info

Publication number
CA2374505A1
CA2374505A1 CA002374505A CA2374505A CA2374505A1 CA 2374505 A1 CA2374505 A1 CA 2374505A1 CA 002374505 A CA002374505 A CA 002374505A CA 2374505 A CA2374505 A CA 2374505A CA 2374505 A1 CA2374505 A1 CA 2374505A1
Authority
CA
Canada
Prior art keywords
phage
filamentous phage
protein
library
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002374505A
Other languages
French (fr)
Other versions
CA2374505C (en
Inventor
Kim D. Janda
Peter Wirsching
Richard A. Lerner
Changshou Gao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2374505A1 publication Critical patent/CA2374505A1/en
Application granted granted Critical
Publication of CA2374505C publication Critical patent/CA2374505C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paper (AREA)

Abstract

The invention describes the display of exogenous polypeptides on filamentous phage using a fusion between the exogenous polypeptide and phage pVII or pIX proteins. In particular, phage particles and phagemid vectors are described for expression and display of heterodimeric proteins such as antibody Fv heterodimers in combinatorial libraries, and uses thereof.

Claims (27)

1. A filamentous phage encapsulating a genome encoding a fusion polypeptide, wherein said polypeptide comprises an exogenous polypeptide fused to the amino terminus of a filamentous phage pVII or pIX protein.
2. The filamentous phage of claim 1 wherein said phage comprises said fusion polypeptide on the phage particle surface.
3. The filamentous phage of claim 1 wherein said fusion polypeptide is a first fusion polypeptide fused to said pVII
protein and said genome further encodes a second fusion polypeptide, wherein said second fusion polypeptide comprises a second exogenous polypeptide fused to the amino terminus of said pIX protein.
4. The filamentous phage of claim 3 wherein said phage comprises said first and second fusion polypeptides on the phage particle surface.
5. The filamentous phage of claim 1 wherein said exogenous polypeptide is selected from the group consisting of an immunoglobulin heavy chain variable domain (V H), an immunoglobulin light chain variable domain (V L), a synthetic polypeptide and a single chain antibody (scFv).
6. The filamentous phage of claim 3 wherein said first and second exogenous polypeptides comprise the first and second polypeptides of a heterodimeric protein complex.
7. The filamentous phage of claim 6 wherein said heterodimeric protein complex is an immunoglobulin Fv, a catalytic Fv, a receptor, a nucleic acid binding protein or an enzyme.
8. The filamentous phage of claim 1 wherein said genome is pCGMT or pCGMT-1b having a nucleotide sequence shown in SEQ
ID NO 19 or 20, respectively.
9. The filamentous phage of claim 7 wherein said Fv is 21H3-V H V L, 2H6-V H V L, or 92H2-V H V L.
10. A vector for expressing a fusion protein on the surface of a filamentous phage comprising a cassette for expressing said fusion protein that includes upstream and downstream translatable DNA sequences operatively linked via a sequence of nucleotides adapted for directional ligation of an insert DNA, said upstream sequence encoding a prokaryotic secretion signal, said downstream sequence encoding a filamentous phage protein selected from the group consisting of pVII and pIX protein, said translatable DNA
sequences operatively linked to a set of DNA expression signals for expression of said translatable DNA sequences as portions of said fusion polypeptide.
11. The vector of claim 10 further comprising a second cassette for expressing a second fusion protein on the surface of said filamentous phage, wherein said second cassette has the structure of said first cassette with the proviso that said first fusion protein expression cassette encodes pVII
protein and said second fusion protein expression cassette encodes pIX protein.
12. The vector of claim 10 wherein said vector is pCGMT
and has a nucleotide sequence shown in SEQ ID NO 19.
13. The vector of claim 11 wherein said vector is pCGMT-lb and has a nucleotide sequence shown in SEQ ID NO 20.
14. The vector of claim 10 wherein said prokaryotic secretion signal is a pelB or ompA secretion signal.
15. The vector of claim 10 further comprising a filamentous phage origin of replication.
16. The vector of claim 10 wherein said set of expression signals includes a promoter, a ribosome binding site, and at least one stop codon in frame with said downstream translatable DNA sequence.
17. A library of filamentous phage particles wherein each phage particle is according to claim 3.
18. A library of filamentous phage particles wherein each phage particle contains a vector according to claim 10.
19. A library of filamentous phage particles wherein each phage particle contains a vector according to claim 11.
20. The library of claim 18 wherein said library contains at least 10' different species of said vector.
21. The library of claim 19 wherein said library contains at least 10 7 different species of said vector.
22. A fusion protein comprising first and second polypeptides wherein said first polypeptide is an exogenous protein having a prokaryotic secretion signal and said second polypeptide is a filamentous phage pVII or pIX protein, whereby said exogenous protein is fused to the amino terminus of said filamentous phage protein.
23. The fusion protein of claim 22 wherein said exogenous polypeptide is selected from the group consisting of an immunoglobulin heavy chain variable domain (V H), an immunoglobulin light chain variable domain (V L), a synthetic polypeptide and a single chain antibody (scFv).
24. A method for changing the diversity of a library of filamentous phage particles comprising the steps of:
a) providing a library of filamentous phage particles according to claim 19;
b) contacting the provided library with a preselected ligand under conditions sufficient for members of the library to bind to the ligand and form a ligand-phage particle complex; and c) isolating phage particles in said complex away from non-bound library members to form a ligand-enriched library comprising phage particles having binding specificity for said preselected ligand.
25. The method of claim 24 wherein said preselected ligand is affixed to a solid support, said complex is in the solid phase and said isolating comprises the steps of:
i) washing the solid support to rinse non-bound library members from the solid support; and ii) eluting solid-phase bound phage particles to form said isolated phage particles.
26. The method of claim 25 wherein said eluting comprises contacting said solid-phase bound phage particles with an elution buffer having a pH of from pH 2 to pH 6.
27. The method of claim 25 wherein said elution comprises contacting said solid-phase bound phage particles with an elution buffer containing said preselected ligand.
CA2374505A 1999-05-25 2000-05-24 Methods for display of heterodimeric proteins on filamentous phage using pvii and pix, compositions, vectors and combinatorial libraries Expired - Fee Related CA2374505C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/318,786 US6472147B1 (en) 1999-05-25 1999-05-25 Methods for display of heterodimeric proteins on filamentous phage using pVII and pIX, compositions, vectors and combinatorial libraries
US09/318,786 1999-05-25
PCT/US2000/014433 WO2000071694A1 (en) 1999-05-25 2000-05-24 METHODS FOR DISPLAY OF HETERODIMERIC PROTEINS ON FILAMENTOUS PHAGE USING pVII and pIX, COMPOSITIONS, VECTORS AND COMBINATORIAL LIBRARIES

Publications (2)

Publication Number Publication Date
CA2374505A1 true CA2374505A1 (en) 2000-11-30
CA2374505C CA2374505C (en) 2011-10-25

Family

ID=23239573

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2374505A Expired - Fee Related CA2374505C (en) 1999-05-25 2000-05-24 Methods for display of heterodimeric proteins on filamentous phage using pvii and pix, compositions, vectors and combinatorial libraries

Country Status (10)

Country Link
US (2) US6472147B1 (en)
EP (1) EP1185636B1 (en)
AT (1) ATE317008T1 (en)
AU (1) AU782349B2 (en)
CA (1) CA2374505C (en)
DE (1) DE60025824T2 (en)
DK (1) DK1185636T3 (en)
ES (1) ES2256015T3 (en)
PT (1) PT1185636E (en)
WO (1) WO2000071694A1 (en)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003091425A1 (en) * 2002-04-26 2003-11-06 Alexion Pharmaceuticals Inc. Novel 33 phage vectors
ES2255616T3 (en) 2001-04-27 2006-07-01 Alexion Pharmaceuticals, Inc. FAGEMIDATE VECTORS.
US20050164515A9 (en) * 2001-06-05 2005-07-28 Belcher Angela M. Biological control of nanoparticle nucleation, shape and crystal phase
US20030148380A1 (en) * 2001-06-05 2003-08-07 Belcher Angela M. Molecular recognition of materials
US20030113714A1 (en) * 2001-09-28 2003-06-19 Belcher Angela M. Biological control of nanoparticles
CA2462732A1 (en) * 2001-10-01 2003-04-10 Buck Institute Target assisted iterative screening (tais) : a novel screening format for large molecular repertoires
US20030073104A1 (en) * 2001-10-02 2003-04-17 Belcher Angela M. Nanoscaling ordering of hybrid materials using genetically engineered mesoscale virus
EP1474161A4 (en) * 2002-01-16 2005-06-29 Zyomyx Inc Engineered binding proteins
CA2965865C (en) 2002-07-18 2021-10-19 Merus N.V. Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
WO2004016782A1 (en) * 2002-08-16 2004-02-26 The University Of Tokyo Method of assaying interaction between proteins
US20050064508A1 (en) * 2003-09-22 2005-03-24 Semzyme Peptide mediated synthesis of metallic and magnetic materials
DE10256669B4 (en) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Mixture of at least two fusion proteins and their preparation and use
DE602004031589D1 (en) 2003-01-07 2011-04-14 Dyax Corp Kunitz DOMAIN LIBRARY
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP2395016A3 (en) 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
US20040266993A1 (en) * 2003-06-30 2004-12-30 Evans Glen A. Non-immunoglobulin binding polypeptides
US7923109B2 (en) * 2004-01-05 2011-04-12 Board Of Regents, The University Of Texas System Inorganic nanowires
DK1737971T3 (en) 2004-01-20 2017-11-13 Merus Nv MIXTURES OF BINDING PROTEINS
JP4937138B2 (en) 2005-01-05 2012-05-23 エフ−シュタール・ビオテヒノロギシェ・フォルシュングス−ウント・エントヴィックルングスゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Synthetic immunoglobulin domains with binding properties designed in a region of the molecule that is different from the complementarity-determining region
AU2005333666A1 (en) * 2005-06-28 2007-01-04 The Scripps Research Institute Delivery of active proteins to the central nervous system using phage vectors
EP1743938A1 (en) * 2005-07-12 2007-01-17 NascaCell IP GmbH Means and methods for displaying polypeptides on cells
KR20140072201A (en) 2006-07-05 2014-06-12 카탈리스트 바이오사이언시즈, 인코포레이티드 Protease screening methods and proteases identified thereby
AT503889B1 (en) 2006-07-05 2011-12-15 Star Biotechnologische Forschungs Und Entwicklungsges M B H F MULTIVALENT IMMUNE LOBULINE
ES2975748T3 (en) 2007-06-26 2024-07-12 F Star Therapeutics Ltd Presentation of bonding agents
US8735330B2 (en) * 2007-08-20 2014-05-27 Nextera As pVII phage display
AU2008343589A1 (en) 2007-12-19 2009-07-09 Centocor Ortho Biotech Inc. Design and generation of human de novo pIX phage display libraries via fusion to pIX or pVII, vectors, antibodies and methods
WO2009085468A2 (en) * 2007-12-19 2009-07-09 Centocor, Inc. Engineered phage vectors for the design and the generation of a human non-antibody peptide or protein phage library via fusion to pix of m13 phage
JP2011507520A (en) * 2007-12-19 2011-03-10 セントコア・オーソ・バイオテツク・インコーポレーテツド Engineered hybrid phage vector for designing and generating human non-antibody peptide or protein phage libraries by fusion of M13 phage with pIX
CN104479017A (en) 2008-04-25 2015-04-01 戴埃克斯有限公司 Antibodies against FCRN and use thereof
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
WO2010021874A2 (en) 2008-08-20 2010-02-25 Centocor Ortho Biotech Inc. Engineered anti-il-13 antibodies, compositions, methods and uses
US9023594B2 (en) 2008-09-05 2015-05-05 President And Fellows Of Harvard College Continuous directed evolution of proteins and nucleic acids
AU2010217551B2 (en) * 2009-02-25 2014-11-27 Nextera As Signal sequence-independent pIX phage display
GB0903316D0 (en) 2009-02-26 2009-04-08 Affitech As Method
IN2012DN01328A (en) 2009-08-13 2015-06-05 Crucell Holland Bv
WO2011035205A2 (en) 2009-09-18 2011-03-24 Calmune Corporation Antibodies against candida, collections thereof and methods of use
US9598692B2 (en) * 2009-09-25 2017-03-21 University Of Oslo Multivalent phage display systems and methods
US8580714B2 (en) 2009-10-14 2013-11-12 Janssen Biotech, Inc. Methods of affinity maturing antibodies
CA2781019A1 (en) * 2009-11-17 2011-05-26 Janssen Biotech, Inc. Display of disulfide linked dimeric proteins on filamentous phage
US8450086B2 (en) 2009-11-17 2013-05-28 Centocor Ortho Biotech Inc. Bacterial membrane protein secretion
US8629106B2 (en) * 2010-03-16 2014-01-14 Janssen Biotech, Inc. Affinity peptides toward BMP-2
PT2571531T (en) 2010-04-30 2016-08-31 Janssen Biotech Inc Stabilized fibronectin domain compositions, methods and uses
CN103097412B (en) 2010-07-09 2016-08-10 克鲁塞尔荷兰公司 Anti-human respiratory syncytial virus (RSV) antibody and using method
EP3202903B1 (en) 2010-12-22 2020-02-12 President and Fellows of Harvard College Continuous directed evolution
US8112218B2 (en) * 2011-03-10 2012-02-07 Ford Global Technologies, Llc Method for controlling an engine
CN103619353B (en) 2011-06-02 2016-01-06 戴埃克斯有限公司 Fc receptor binding protein
KR102142385B1 (en) 2011-09-27 2020-08-10 얀센 바이오테크 인코포레이티드 Fibronectin type iii repeat based protein scaffolds with alternative binding surfaces
AU2013249985B2 (en) 2012-04-20 2017-11-23 Merus N.V. Methods and means for the production of Ig-like molecules
US8822423B2 (en) 2012-05-17 2014-09-02 Janssen Biotech, Inc. Affinity peptides toward infliximab
US9695228B2 (en) 2012-11-21 2017-07-04 Janssen Biotech, Inc. EGFR and c-Met fibronectin type III domain binding molecules
CA3182876A1 (en) 2012-11-21 2014-05-30 Janssen Biotech, Inc. Bispecific egfr/c-met antibodies
KR102160389B1 (en) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 De novo synthesized gene libraries
WO2015057545A2 (en) 2013-10-14 2015-04-23 Janssen Biotech, Inc. Cysteine engineered fibronectin type iii domain binding molecules
US10179911B2 (en) 2014-01-20 2019-01-15 President And Fellows Of Harvard College Negative selection and stringency modulation in continuous evolution systems
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
MX2017006485A (en) 2014-11-18 2018-03-23 Janssen Pharmaceutica Nv Cd47 antibodies, methods, and uses.
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016168631A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Vector-based mutagenesis system
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844111B2 (en) 2015-05-06 2020-11-24 Janssen Biotech, Inc. Prostate specific membrane antigen binding fibronectin type III domains
WO2016208696A1 (en) 2015-06-24 2016-12-29 Ｊｃｒファーマ株式会社 Fusion protein containing bdnf
KR20180020277A (en) * 2015-06-24 2018-02-27 제이씨알 파마 가부시키가이샤 Anti-Human Transferrin Receptor Antibody Passing Through the Blood Brain Gate
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
KR20180058772A (en) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 Flexible substrate for nucleic acid synthesis
IL294014B2 (en) 2015-10-23 2024-07-01 Harvard College Nucleobase editors and uses thereof
CN115920796A (en) 2015-12-01 2023-04-07 特韦斯特生物科学公司 Functionalized surfaces and preparation thereof
EP3448997B1 (en) 2016-04-27 2020-10-14 Massachusetts Institute of Technology Stable nanoscale nucleic acid assemblies and methods thereof
US11514331B2 (en) 2016-04-27 2022-11-29 Massachusetts Institute Of Technology Sequence-controlled polymer random access memory storage
US10925932B2 (en) 2016-06-03 2021-02-23 Janssen Biotech, Inc. Serum albumin-binding fibronectin type III domains
EP3471750A4 (en) 2016-06-21 2020-02-26 Janssen Biotech, Inc. Cysteine engineered fibronectin type iii domain binding molecules
US20200182884A1 (en) 2016-06-27 2020-06-11 Juno Therapeutics, Inc. Method of identifying peptide epitopes, molecules that bind such epitopes and related uses
MA45491A (en) 2016-06-27 2019-05-01 Juno Therapeutics Inc CMH-E RESTRICTED EPITOPES, BINDING MOLECULES AND RELATED METHODS AND USES
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
JP7088932B2 (en) 2016-09-14 2022-06-21 ヤンセン バイオテツク，インコーポレーテツド Chimeric antigen receptor with BCMA-specific fibronectin type III domain, and its use
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10597438B2 (en) 2016-12-14 2020-03-24 Janssen Biotech, Inc. PD-L1 binding fibronectin type III domains
WO2018111978A1 (en) 2016-12-14 2018-06-21 Janssen Biotech, Inc. Cd137 binding fibronectin type iii domains
CA3046963A1 (en) 2016-12-14 2018-06-21 Janssen Biotech, Inc. Cd8a-binding fibronectin type iii domains
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
BR112019009316A2 (en) 2016-12-26 2019-08-06 Japan Chem Res human transferrin anti-receptor antibody, fusion protein, DNA fragment, expression vector, mammalian cell, pharmacologically active compound and human transferrin anti-receptor antibody complex, uses of human transferrin anti-receptor antibody and fusion protein, and, methods for treating a central nervous system disorder and a central nervous system disorder accompanying pompe disease, hurler syndrome or hurler-scheie syndrome.
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
WO2019040935A1 (en) 2017-08-25 2019-02-28 President And Fellows Of Harvard College Evolution of bont peptidases
CN111566125A (en) 2017-09-11 2020-08-21 特韦斯特生物科学公司 GPCR binding proteins and synthesis thereof
WO2019056002A1 (en) 2017-09-18 2019-03-21 President And Fellows Of Harvard College Continuous evolution for stabilized proteins
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
EP3707150A1 (en) 2017-11-10 2020-09-16 Massachusetts Institute Of Technology Microbial production of pure single stranded nucleic acids
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
CN112639130B (en) 2018-05-18 2024-08-09 特韦斯特生物科学公司 Polynucleotides, reagents and methods for nucleic acid hybridization
WO2019241649A1 (en) 2018-06-14 2019-12-19 President And Fellows Of Harvard College Evolution of cytidine deaminases
WO2020154595A1 (en) 2019-01-24 2020-07-30 Massachusetts Institute Of Technology Nucleic acid nanostructure platform for antigen presentation and vaccine formulations formed therefrom
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
JP2022522668A (en) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション Mutant nucleic acid library for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11905532B2 (en) 2019-06-25 2024-02-20 Massachusetts Institute Of Technology Compositions and methods for molecular memory storage and retrieval
AU2020356471A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
WO2021076546A1 (en) 2019-10-14 2021-04-22 Aro Biotherapeutics Company Cd71 binding fibronectin type iii domains
US11781138B2 (en) 2019-10-14 2023-10-10 Aro Biotherapeutics Company FN3 domain-siRNA conjugates and uses thereof
JP2023523336A (en) 2020-04-27 2023-06-02 ツイスト バイオサイエンス コーポレーション Mutant nucleic acid library for coronavirus
CN111793135A (en) * 2020-05-11 2020-10-20 廊坊天光生物技术有限公司 Antibody pair for detecting RANKL content in serum and application thereof
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
BR112023021325A2 (en) 2021-04-14 2023-12-19 Aro Biotherapeutics Company CD71-BINDING TYPE III FIBRONECTIN DOMAINS

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
DE4122599C2 (en) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid for screening antibodies
GB9500851D0 (en) 1995-01-17 1995-03-08 Bionvent International Ab Method of selecting specific bacteriophages
US6054312A (en) 1997-08-29 2000-04-25 Selective Genetics, Inc. Receptor-mediated gene delivery using bacteriophage vectors
WO2001005950A2 (en) * 1999-07-20 2001-01-25 Morphosys Ag Methods for displaying (poly)peptides/proteins on bacteriophage particles via disulfide bonds

Also Published As

Publication number Publication date
EP1185636A1 (en) 2002-03-13
US7078166B2 (en) 2006-07-18
EP1185636B1 (en) 2006-02-01
AU782349B2 (en) 2005-07-21
EP1185636A4 (en) 2004-10-06
DE60025824D1 (en) 2006-04-13
CA2374505C (en) 2011-10-25
US20030186322A1 (en) 2003-10-02
US6472147B1 (en) 2002-10-29
PT1185636E (en) 2006-05-31
DE60025824T2 (en) 2006-09-07
WO2000071694A1 (en) 2000-11-30
ES2256015T3 (en) 2006-07-16
AU5290000A (en) 2000-12-12
ATE317008T1 (en) 2006-02-15
DK1185636T3 (en) 2006-04-18

Similar Documents

Publication Publication Date Title
CA2374505A1 (en) Methods for display of heterodimeric proteins on filamentous phage using pvii and pix, compositions, vectors and combinatorial libraries
EP0527839B1 (en) Recombinant library screening methods
Röttgen et al. A human pancreatic secretory trypsin inhibitor presenting a hypervariable highly constrained epitope via monovalent phagemid display
JP3507073B2 (en) Methods for producing members of a specific binding pair
Barbas 3rd et al. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
US8124742B2 (en) Rh(D)-binding proteins
Breitling et al. A surface expression vector for antibody screening
Wells et al. Rapid evolution of peptide and protein binding properties in vitro
CA2291721A1 (en) Ribosome complexes as selection particles for in vitro display and evolution of proteins
CA2497496A1 (en) In vitro peptide expression library
WO2003010176A2 (en) Protein-nucleic acid complexes
CA2326499A1 (en) Methods of selecting internalizing antibodies
CZ20013399A3 (en) Protein isolation method and protein analysis, particularly mass spectrometry analysis
KR100961392B1 (en) Production method of antibody phage display library, the antibody phage display library produced thereby and the phagemid vector comprising said antibody phage display library
CA2415328A1 (en) Nested sorting and high throughput screening
CA2301826A1 (en) Chimeric binding peptide library screening method
AU722978B2 (en) Magnetically activated cell sorting for production of proteins
Coia et al. Panning and selection of proteins using ribosome display
WO2004013276A9 (en) Novel tricistronic vectors and uses therefor
WO1998016541A9 (en) Magnetically activated cell sorting for production of proteins
Balass et al. Recovery of high-affinity phage from a nitrostreptavidin matrix in phage-display technology
KR100458083B1 (en) Method for the construction of phage display library using helper phage variants
Li et al. Filamentous bacteriophage display of a bifunctional protein A:: scFv fusion
McGregor et al. External surface display of proteins linked to DNA-binding domains
KR20240066998A (en) Method for cell panning

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20190524