CA2369363A1 - Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification - Google Patents
Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification Download PDFInfo
- Publication number
- CA2369363A1 CA2369363A1 CA002369363A CA2369363A CA2369363A1 CA 2369363 A1 CA2369363 A1 CA 2369363A1 CA 002369363 A CA002369363 A CA 002369363A CA 2369363 A CA2369363 A CA 2369363A CA 2369363 A1 CA2369363 A1 CA 2369363A1
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- CA
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- Prior art keywords
- capillary tube
- fluid
- capillary
- array
- biological sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un dispositif automatique, permettant de traiter rapidement des échantillons de la taille du sous-microlitre, par réactions chimiques ou enzymatiques qui impliquent une manipulation de liquide, une ou plusieurs étapes d'incubation à température régulée (notamment le thermocyclage), et une étape de purification basée sur la discrimination de taille moléculaire. On compte, notamment, parmi les applications exemplaires, la réaction en chaîne de la polymérase (PCR), des applications de séquençage d'ADN, la ligation d'oligonucléotides, la ligation répétitive d'oligonucléotides (LCR), l'extension unique de nucléotides, le traitement par un exonucléase, et des dosages d'hybridation d'oligonucléotides.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13166099P | 1999-04-29 | 1999-04-29 | |
US60/131,660 | 1999-04-29 | ||
US15529999P | 1999-09-21 | 1999-09-21 | |
US60/155,299 | 1999-09-21 | ||
PCT/US2000/011371 WO2000066995A2 (fr) | 1999-04-29 | 2000-04-29 | Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2369363A1 true CA2369363A1 (fr) | 2000-11-09 |
Family
ID=26829699
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002369363A Abandoned CA2369363A1 (fr) | 1999-04-29 | 2000-04-29 | Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1177043A2 (fr) |
JP (1) | JP2002543418A (fr) |
AU (1) | AU4672400A (fr) |
CA (1) | CA2369363A1 (fr) |
WO (1) | WO2000066995A2 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030077611A1 (en) | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
US6833238B2 (en) | 2002-01-04 | 2004-12-21 | Applera Corporation | Petal-array support for use with microplates |
US7081339B2 (en) | 2002-04-12 | 2006-07-25 | Primera Biosystems, Inc. | Methods for variation detection |
US7445893B2 (en) | 2002-04-12 | 2008-11-04 | Primera Biosystems, Inc. | Sampling method for amplification reaction analysis |
EP1525052A1 (fr) * | 2002-07-26 | 2005-04-27 | Applera Corporation | Particule d'echange ionique a exclusion de dimension |
CA2509769A1 (fr) * | 2002-11-21 | 2004-06-10 | Primera Biosystems | Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification |
US7651665B2 (en) * | 2004-09-07 | 2010-01-26 | Hewlett-Packard Development Company, L.P. | Microtray for handling biosubstances |
CN103252261B (zh) | 2007-10-30 | 2015-04-15 | 松下健康医疗器械株式会社 | 分析用仪器 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4769216A (en) * | 1980-12-22 | 1988-09-06 | Commonwealth Serum Laboratories Commission | Device for detecting antigens and antibodies |
US5030555A (en) * | 1988-09-12 | 1991-07-09 | University Of Florida | Membrane-strip reagent serodiagnostic apparatus and method |
JPH06265447A (ja) * | 1993-03-16 | 1994-09-22 | Hitachi Ltd | 微量反応装置およびこれを使用する微量成分測定装置 |
US5449064A (en) * | 1994-03-03 | 1995-09-12 | University Of Kansas | On-line interface and valve for capillary electophoresis system |
US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
GB2312750A (en) * | 1996-05-02 | 1997-11-05 | Univ Degli Studi Milano | Differential pH measurement apparatus |
WO1997048818A1 (fr) * | 1996-06-17 | 1997-12-24 | The Board Of Trustees Of The Leland Stanford Junior University | Appareil et procede de thermocyclage |
US6190616B1 (en) * | 1997-09-11 | 2001-02-20 | Molecular Dynamics, Inc. | Capillary valve, connector, and router |
US5961925A (en) * | 1997-09-22 | 1999-10-05 | Bristol-Myers Squibb Company | Apparatus for synthesis of multiple organic compounds with pinch valve block |
-
2000
- 2000-04-29 WO PCT/US2000/011371 patent/WO2000066995A2/fr not_active Application Discontinuation
- 2000-04-29 AU AU46724/00A patent/AU4672400A/en not_active Abandoned
- 2000-04-29 EP EP00928488A patent/EP1177043A2/fr not_active Withdrawn
- 2000-04-29 JP JP2000615584A patent/JP2002543418A/ja not_active Withdrawn
- 2000-04-29 CA CA002369363A patent/CA2369363A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP2002543418A (ja) | 2002-12-17 |
WO2000066995A2 (fr) | 2000-11-09 |
AU4672400A (en) | 2000-11-17 |
WO2000066995A3 (fr) | 2001-07-26 |
EP1177043A2 (fr) | 2002-02-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |