CA2369058A1 - Gene synthetique destine a l'expression d'une proteine retrovirale active dans les eucariotes - Google Patents
Gene synthetique destine a l'expression d'une proteine retrovirale active dans les eucariotes Download PDFInfo
- Publication number
- CA2369058A1 CA2369058A1 CA002369058A CA2369058A CA2369058A1 CA 2369058 A1 CA2369058 A1 CA 2369058A1 CA 002369058 A CA002369058 A CA 002369058A CA 2369058 A CA2369058 A CA 2369058A CA 2369058 A1 CA2369058 A1 CA 2369058A1
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- Prior art keywords
- gene
- protein
- retroviral
- integrase
- gag
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16051—Methods of production or purification of viral material
- C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La présente invention concerne un gène synthétique ou une région de gène ayant une utilisation améliorée de codon par rapport au gène de type de sauvage et servant à l'expression de niveau élevé d'une protéine rétrovirale dans des cellules eucariotes, la protéine rétrovirale exprimée possédant une activité enzymatique dans la cellule eucariote. L'invention concerne également un gène synthétique ou une région de gène codant une enzyme rétrovirale ou une partie d'enzyme exprimée normalement dans une cellule mammalienne ou dans une autre cellule eucariote, au moins un codon non préféré dans le gène de type sauvage codant l'enzyme ayant été remplacé par un codon préféré codant le même acide aminé. La protéine rétrovirale peut être une protéase, une transcriptase inverse, une protéine intégrase ou son précurseur gag-pol de polyprotéine. Dans un mode de réalisation la protéine rétrovirale à activité enzymatique est une protéine lentivirale. Dans d'autres modes de réalisation, la protéine à activité enzymatique est une enzyme pol. Dans des modes de réalisation préférés, la protéine à activité enzymatique est une intégrase lentivirale, ou, mieux, une enzyme de VIH; dans d'autres modes de réalisation, la protéine à activité enzymatique est idéalement une intégrase de VIH. L'invention concerne également un procédé de détection de l'intégrase intracellulaire au moyen d'un gène reporter démuni de promoteur.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99201306 | 1999-04-26 | ||
| EP99201306.0 | 1999-04-26 | ||
| EP00200171.7 | 2000-01-18 | ||
| EP00200171 | 2000-01-18 | ||
| PCT/EP2000/003765 WO2000065076A2 (fr) | 1999-04-26 | 2000-04-26 | Gene synthetique destine a l'expression d'une proteine retrovirale active dans les eucariotes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2369058A1 true CA2369058A1 (fr) | 2000-11-02 |
Family
ID=26071747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002369058A Abandoned CA2369058A1 (fr) | 1999-04-26 | 2000-04-26 | Gene synthetique destine a l'expression d'une proteine retrovirale active dans les eucariotes |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1183381A2 (fr) |
| JP (1) | JP2002542791A (fr) |
| AU (1) | AU778106B2 (fr) |
| BR (1) | BR0010077A (fr) |
| CA (1) | CA2369058A1 (fr) |
| HU (1) | HUP0201019A2 (fr) |
| IL (1) | IL146090A0 (fr) |
| NO (1) | NO20015168L (fr) |
| PL (1) | PL351988A1 (fr) |
| RU (1) | RU2001131728A (fr) |
| WO (1) | WO2000065076A2 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9803351D0 (en) | 1998-02-17 | 1998-04-15 | Oxford Biomedica Ltd | Anti-viral vectors |
| CA2358385C (fr) | 1998-12-31 | 2013-08-06 | Chiron Corporation | Polynucleotides codant pour des polypeptides antigeniques du type c du vih; polypeptides et leurs utilisations |
| US6656706B2 (en) | 1999-12-23 | 2003-12-02 | The United States Of America As Represented By The Department Of Health And Human Services | Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes |
| DE60042702D1 (de) * | 1999-12-23 | 2009-09-17 | Us Gov Health & Human Serv | Molekulare klone mit mutierten hiv gag/pol, siv gag und siv env gene |
| AUPQ580300A0 (en) * | 2000-02-23 | 2000-03-16 | National Cancer Centre Of Singapore Pte Ltd | Genetic analysis |
| GB0009760D0 (en) * | 2000-04-19 | 2000-06-07 | Oxford Biomedica Ltd | Method |
| EP1411770A4 (fr) | 2001-07-05 | 2006-05-10 | Chiron Corp | Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations |
| US6835568B2 (en) | 2001-10-30 | 2004-12-28 | Virxsys Corporation | Regulated nucleic acid expression system |
| DE602004030104D1 (de) * | 2003-05-06 | 2010-12-30 | Nat Inst Of Advanced Ind Scien | System zur bestimmung der aktivität mehrerer gentranskriptionen |
| ATE521629T1 (de) | 2005-10-07 | 2011-09-15 | Angeletti P Ist Richerche Bio | Matrixmetalloproteinase-11-impfstoff |
| JP6824594B2 (ja) | 2014-09-11 | 2021-02-03 | Jnc株式会社 | 合成遺伝子の設計方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5468629A (en) * | 1993-04-13 | 1995-11-21 | Calhoun; Cornelia | Method of promoting in vitro homologous recombination transfection in mammalian cells using the RecA protein |
| US5434065A (en) * | 1993-05-06 | 1995-07-18 | President And Fellows Of Harvard College | In vivo selection of microbial virulence genes |
| US5811270A (en) * | 1994-05-20 | 1998-09-22 | Grandgenett; Duane P. | In vitro method for concerted integration of donor DNA molecules using retroviral integrase proteins |
| US6200811B1 (en) * | 1996-04-02 | 2001-03-13 | The Regents Of The University Of California | Cell transformation vector comprising an HIV-2 packaging site nucleic acid and an HIV-1 GAG protein |
| US6114148C1 (en) * | 1996-09-20 | 2012-05-01 | Gen Hospital Corp | High level expression of proteins |
| IL131131A0 (en) * | 1997-02-07 | 2001-01-28 | Merck & Co Inc | Synthetic hiv gag genes |
| EP1141313A2 (fr) * | 1998-12-31 | 2001-10-10 | Chiron Corporation | Expression amelioree de polypeptides hiv et production de particules de type viral |
| CA2358385C (fr) * | 1998-12-31 | 2013-08-06 | Chiron Corporation | Polynucleotides codant pour des polypeptides antigeniques du type c du vih; polypeptides et leurs utilisations |
-
2000
- 2000-04-26 CA CA002369058A patent/CA2369058A1/fr not_active Abandoned
- 2000-04-26 BR BR0010077-3A patent/BR0010077A/pt not_active Application Discontinuation
- 2000-04-26 HU HU0201019A patent/HUP0201019A2/hu unknown
- 2000-04-26 EP EP00927069A patent/EP1183381A2/fr not_active Withdrawn
- 2000-04-26 JP JP2000614410A patent/JP2002542791A/ja not_active Withdrawn
- 2000-04-26 AU AU45579/00A patent/AU778106B2/en not_active Ceased
- 2000-04-26 RU RU2001131728/15A patent/RU2001131728A/ru unknown
- 2000-04-26 PL PL00351988A patent/PL351988A1/xx unknown
- 2000-04-26 WO PCT/EP2000/003765 patent/WO2000065076A2/fr active IP Right Grant
- 2000-04-26 IL IL14609000A patent/IL146090A0/xx unknown
-
2001
- 2001-10-23 NO NO20015168A patent/NO20015168L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002542791A (ja) | 2002-12-17 |
| EP1183381A2 (fr) | 2002-03-06 |
| PL351988A1 (en) | 2003-07-14 |
| AU4557900A (en) | 2000-11-10 |
| AU778106B2 (en) | 2004-11-18 |
| NO20015168L (no) | 2001-12-14 |
| WO2000065076A3 (fr) | 2001-12-13 |
| IL146090A0 (en) | 2002-07-25 |
| NO20015168D0 (no) | 2001-10-23 |
| WO2000065076A2 (fr) | 2000-11-02 |
| HUP0201019A2 (en) | 2002-08-28 |
| RU2001131728A (ru) | 2004-02-20 |
| BR0010077A (pt) | 2002-01-15 |
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Legal Events
| Date | Code | Title | Description |
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| FZDE | Discontinued |