CA2347214A1 - Automatisation de la conception des proteines pour l'elaboration des bibliotheques de proteines - Google Patents

Automatisation de la conception des proteines pour l'elaboration des bibliotheques de proteines Download PDF

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Publication number
CA2347214A1
CA2347214A1 CA002347214A CA2347214A CA2347214A1 CA 2347214 A1 CA2347214 A1 CA 2347214A1 CA 002347214 A CA002347214 A CA 002347214A CA 2347214 A CA2347214 A CA 2347214A CA 2347214 A1 CA2347214 A1 CA 2347214A1
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CA
Canada
Prior art keywords
library
protein
sequences
sequence
positions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002347214A
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English (en)
Inventor
Klaus M. Fiebig
Robert J. Hayes
Bassil I. Dahiyat
Jorg Bentzien
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Xencor Inc
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Individual
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Publication date
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Publication of CA2347214A1 publication Critical patent/CA2347214A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne l'automatisation de la conception des protéines pour l'élaboration de bibliothèques secondaires de protéines présélectionnées par calcul, et elle concerne également des procédés et des compositions associés à l'utilisation des bibliothèques.
CA002347214A 1998-10-16 1999-10-15 Automatisation de la conception des proteines pour l'elaboration des bibliotheques de proteines Abandoned CA2347214A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10461298P 1998-10-16 1998-10-16
US60/104,612 1998-10-16
US15870099P 1999-10-08 1999-10-08
US60/158,700 1999-10-08
PCT/US1999/024229 WO2000023564A2 (fr) 1998-10-16 1999-10-15 Automatisation de la conception des proteines pour l'elaboration des bibliotheques de proteines

Publications (1)

Publication Number Publication Date
CA2347214A1 true CA2347214A1 (fr) 2000-04-27

Family

ID=26801737

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002347214A Abandoned CA2347214A1 (fr) 1998-10-16 1999-10-15 Automatisation de la conception des proteines pour l'elaboration des bibliotheques de proteines

Country Status (5)

Country Link
EP (1) EP1157093A1 (fr)
JP (1) JP2003527072A (fr)
AU (2) AU774334B2 (fr)
CA (1) CA2347214A1 (fr)
WO (1) WO2000023564A2 (fr)

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JP2002510966A (ja) 1997-04-11 2002-04-09 カリフォルニア・インスティテュート・オブ・テクノロジー 自動蛋白質デザインのための装置および方法
US7315786B2 (en) 1998-10-16 2008-01-01 Xencor Protein design automation for protein libraries
US20020048772A1 (en) 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US6946265B1 (en) 1999-05-12 2005-09-20 Xencor, Inc. Nucleic acids and proteins with growth hormone activity
US6746853B1 (en) 1999-05-19 2004-06-08 Xencor, Inc. Proteins with insulin-like activity useful in the treatment of diabetes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
JP2003521933A (ja) * 2000-02-10 2003-07-22 ゼンコー タンパク質ライブラリーに関するタンパク質設計オートメーション
EP1621617A1 (fr) * 2000-02-10 2006-02-01 Xencor, Inc. Dessin automatisé de protéines pour l'élaboration de bibliothèques de protéines
US7101974B2 (en) 2000-03-02 2006-09-05 Xencor TNF-αvariants
US7687461B2 (en) 2000-03-02 2010-03-30 Xencor, Inc. Treatment of TNF-α related disorders with TNF-α variant proteins
US7446174B2 (en) 2001-03-02 2008-11-04 Xencor, Inc. Protein based TNF-α variants for the treatment of TNF-α related disorders
US7662367B2 (en) 2000-03-02 2010-02-16 Xencor, Inc. Pharmaceutical compositions for the treatment of TNF-α related disorders
US7244823B2 (en) 2000-03-02 2007-07-17 Xencor TNF-alpha variants proteins for the treatment of TNF-alpha related disorders
US7056695B2 (en) 2000-03-02 2006-06-06 Xencor TNF-α variants
AU2001278872A1 (en) 2000-07-06 2002-01-21 Genvec, Inc. Method of identifying a binding partner of a gene product
EP1299950B1 (fr) 2000-07-13 2008-05-21 CT-Concept Technologie AG Procede et dispositif pour le reglage, en fonction de l'etat, du comportement transitoire de commutateurs a semi-conducteur de puissance
EP1325457A4 (fr) * 2000-10-10 2007-10-24 Genencor Int Bibliotheques riches en informations
US7351690B2 (en) 2000-12-19 2008-04-01 Palatin Technologies, Inc. Knockout identification of target-specific sites in peptides
WO2002068453A2 (fr) * 2001-02-22 2002-09-06 Xencor Procedes et compositions pour la realisation et l'utilisation de librairies de fusion, au moyen de techniques d'elaboration informatique de proteines
WO2002090300A2 (fr) 2001-05-04 2002-11-14 Xencor Acides nucleiques et proteines presentant une activite de thioredoxine reductase
US7381792B2 (en) 2002-01-04 2008-06-03 Xencor, Inc. Variants of RANKL protein
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
US8188231B2 (en) 2002-09-27 2012-05-29 Xencor, Inc. Optimized FC variants
US7662925B2 (en) 2002-03-01 2010-02-16 Xencor, Inc. Optimized Fc variants and methods for their generation
US7317091B2 (en) 2002-03-01 2008-01-08 Xencor, Inc. Optimized Fc variants
DE10233047A1 (de) * 2002-07-19 2004-02-26 Amaxa Gmbh Verfahren zur Herstellung eines künstlichen Polypeptids und nach diesem Verfahren hergestelltes künstliches Protein
US8084582B2 (en) 2003-03-03 2011-12-27 Xencor, Inc. Optimized anti-CD20 monoclonal antibodies having Fc variants
US20090010920A1 (en) 2003-03-03 2009-01-08 Xencor, Inc. Fc Variants Having Decreased Affinity for FcyRIIb
US8388955B2 (en) 2003-03-03 2013-03-05 Xencor, Inc. Fc variants
US9051373B2 (en) 2003-05-02 2015-06-09 Xencor, Inc. Optimized Fc variants
US9714282B2 (en) 2003-09-26 2017-07-25 Xencor, Inc. Optimized Fc variants and methods for their generation
US8101720B2 (en) 2004-10-21 2012-01-24 Xencor, Inc. Immunoglobulin insertions, deletions and substitutions
EP2221315A1 (fr) 2003-12-04 2010-08-25 Xencor, Inc. Procédés de génération de protéines variantes avec un contenu amélioré de fil hôte et compositions associées
US20150010550A1 (en) 2004-07-15 2015-01-08 Xencor, Inc. OPTIMIZED Fc VARIANTS
US8802820B2 (en) 2004-11-12 2014-08-12 Xencor, Inc. Fc variants with altered binding to FcRn
US8367805B2 (en) 2004-11-12 2013-02-05 Xencor, Inc. Fc variants with altered binding to FcRn
JP4652414B2 (ja) 2004-11-12 2011-03-16 ゼンコー・インコーポレイテッド ＦｃＲｎとの変化した結合を有するＦｃ変異体
US8546543B2 (en) 2004-11-12 2013-10-01 Xencor, Inc. Fc variants that extend antibody half-life
WO2006072563A2 (fr) * 2005-01-03 2006-07-13 F. Hoffmann-La Roche Ag Structure similaire a de l'hemopexine en tant que nouvel echafaudage polypeptidique
EP1931709B1 (fr) 2005-10-03 2016-12-07 Xencor, Inc. Variants de fc dotés de propriétés de liaison aux récepteurs fc optimisées
AU2006302254B2 (en) 2005-10-06 2011-05-26 Xencor, Inc. Optimized anti-CD30 antibodies
DK2059536T3 (da) 2006-08-14 2014-04-14 Xencor Inc Optimerede antistoffer, der er rettet mod cd19
CA2660795C (fr) 2006-09-18 2014-11-18 Xencor, Inc. Anticorps optimises ciblant l'antigene hm1.24
PL2808343T3 (pl) 2007-12-26 2019-11-29 Xencor Inc Warianty Fc ze zmienionym wiązaniem do FcRn
JP5703021B2 (ja) 2008-09-26 2015-04-15 日本水産株式会社 脂質の濃縮方法
US9493578B2 (en) 2009-09-02 2016-11-15 Xencor, Inc. Compositions and methods for simultaneous bivalent and monovalent co-engagement of antigens
WO2011091078A2 (fr) 2010-01-19 2011-07-28 Xencor, Inc. Variants d'anticorps possédant une activité complémentaire accrue

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US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP2002510966A (ja) * 1997-04-11 2002-04-09 カリフォルニア・インスティテュート・オブ・テクノロジー 自動蛋白質デザインのための装置および方法
AU5132700A (en) * 1999-05-12 2000-11-21 Xencor, Inc. Novel thermostable alkaliphilic xylanase

Also Published As

Publication number Publication date
AU2004203224B2 (en) 2007-07-05
AU2004203224A1 (en) 2004-08-12
AU1119000A (en) 2000-05-08
JP2003527072A (ja) 2003-09-16
AU774334B2 (en) 2004-06-24
WO2000023564A3 (fr) 2001-12-13
EP1157093A1 (fr) 2001-11-28
WO2000023564A2 (fr) 2000-04-27

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Legal Events

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EEER Examination request
FZDE Dead