CA2342831A1 - Enzyme biosynthetique d'acides gras a chaines longues de plantes - Google Patents

Enzyme biosynthetique d'acides gras a chaines longues de plantes Download PDF

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Publication number
CA2342831A1
CA2342831A1 CA002342831A CA2342831A CA2342831A1 CA 2342831 A1 CA2342831 A1 CA 2342831A1 CA 002342831 A CA002342831 A CA 002342831A CA 2342831 A CA2342831 A CA 2342831A CA 2342831 A1 CA2342831 A1 CA 2342831A1
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CA
Canada
Prior art keywords
nucleic acid
kcs2
sequence
arabidopsis
coding sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002342831A
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English (en)
Inventor
Ljerka Kunst
Sabine Clemens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2342831A1 publication Critical patent/CA2342831A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Selon divers aspects, la présente invention concerne une séquence d'acides nucléiques codant tout ou partie d'une nouvelle enzyme condensant les acides gras à chaînes longues (élongase d'acide gras), désignée ci-après KCS2 (pour béta-kétoacyl-coenzyme A synthase 2). Dans certains modes de réalisation, KCS2 peut induire la biosynthèse d'acides gras C18, C20, C22 et C24. L'activité de cette enzyme est généralement caractérisée par les additions de deux carbone(malonyl-CoA) aux fractions C16, C18, C20 et C22 (molécules acylCoA C16-C22), c'est-à-dire, la condensation de malonyl-CoA avec un acyl-CoA C16, C18, C20 ou C22. Les acides gras produits par l'enzyme peuvent, par exemple, être des acides gras 18 :0, 20 :0, 22 :0 et 24 :0 saturés. L'invention concerne des molécules d'acide nucléique de recombinaison comprenant une séquence de codage d'acides nucléiques hétérologues codant l'enzyme de condensation d'acides gras à chaînes longues de plantes.
CA002342831A 1999-07-22 2000-07-21 Enzyme biosynthetique d'acides gras a chaines longues de plantes Abandoned CA2342831A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14501399P 1999-07-22 1999-07-22
US60/145,013 1999-07-22
PCT/CA2000/000860 WO2001007586A2 (fr) 1999-07-22 2000-07-21 Enzyme biosynthetique d'acides gras a chaines longues de plantes

Publications (1)

Publication Number Publication Date
CA2342831A1 true CA2342831A1 (fr) 2001-02-01

Family

ID=22511209

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002342831A Abandoned CA2342831A1 (fr) 1999-07-22 2000-07-21 Enzyme biosynthetique d'acides gras a chaines longues de plantes

Country Status (3)

Country Link
AU (1) AU6418000A (fr)
CA (1) CA2342831A1 (fr)
WO (1) WO2001007586A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001090386A2 (fr) * 2000-05-24 2001-11-29 The University Of British Columbia Region regulatrice de genes promouvant une transcription precoce specifique de graines
CA2409885A1 (fr) * 2000-05-24 2001-11-29 The University Of British Columbia Acide nucleique codant un enzyme biosynthetique d'acide gras possedant une chaine tres longue et appartenant a une plante
CA2448112A1 (fr) * 2001-06-05 2002-12-12 Exelixis Inc. Lce utilises comme modificateurs de la voie p53 et procede d'utilisation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679881A (en) * 1991-11-20 1997-10-21 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism
ATE276368T1 (de) * 1994-10-26 2004-10-15 Cargill Inc Fae1gene und deren anwendungen

Also Published As

Publication number Publication date
AU6418000A (en) 2001-02-13
WO2001007586A2 (fr) 2001-02-01
WO2001007586A3 (fr) 2001-08-23

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued