CA2340623A1 - Methode pour l'identification et la representation de differences interindividuelles de sequences d'adn - Google Patents

Methode pour l'identification et la representation de differences interindividuelles de sequences d'adn Download PDF

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Publication number
CA2340623A1
CA2340623A1 CA002340623A CA2340623A CA2340623A1 CA 2340623 A1 CA2340623 A1 CA 2340623A1 CA 002340623 A CA002340623 A CA 002340623A CA 2340623 A CA2340623 A CA 2340623A CA 2340623 A1 CA2340623 A1 CA 2340623A1
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CA
Canada
Prior art keywords
sequencing
dna
sequences
gene segments
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002340623A
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English (en)
Inventor
Margret Hoehe
Karla Kopke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genprofile AG
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2340623A1 publication Critical patent/CA2340623A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode pour l'identification et la représentation de différences interindividuelles de séquences d'ADN. Elle est caractérisée en ce que l'on isole l'ADN génomique des individus à analyser, on définit des segments des gènes, des régions génomiques et des génomes à comparer, on isole de manière ciblée ces segments au moyen de l'amplification en chaîne par polymérase en utilisant des oligonucléotides très spécifiques, et on en fabrique un nombre suffisant de copies, on réalise un séquençage simultané de tous les segments géniques obtenus qui se présentent désormais sous la forme de produits d'amplification en chaîne par polymérase, on sépare ensuite par voie électrophorétique sur un gel de séquençage le mélange ainsi formé issu des réactions de terminaison et contenant les séquences individuelles de tous les segments géniques, et on le transfère sur une membrane par électrophorèse directe de transfert, puis on met en évidence, par hybridation de la membrane avec un oligonucléotide très spécifique marqué, les séquences individuelles d'un segment génique déterminé provenant du mélange initial, on répète cette opération jusqu'à ce que tous les segments géniques provenant du groupe initial soient détectés, et on détecte ensuite par analyse d'image les différences interindividuelles de séquences d'ADN, et on établit et on évalue par traitement mathématique un modèle de profil génique.
CA002340623A 1998-05-06 1999-05-03 Methode pour l'identification et la representation de differences interindividuelles de sequences d'adn Abandoned CA2340623A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19821114A DE19821114A1 (de) 1998-05-06 1998-05-06 Methode zur Identifizierung und Darstellung interindividueller DNA-Sequenzunterschiede
DE19821114.7 1998-05-06
PCT/DE1999/001385 WO1999057308A1 (fr) 1998-05-06 1999-05-03 Methode pour l'identification et la representation de differences interindividuelles de sequences d'adn

Publications (1)

Publication Number Publication Date
CA2340623A1 true CA2340623A1 (fr) 1999-11-11

Family

ID=7867445

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002340623A Abandoned CA2340623A1 (fr) 1998-05-06 1999-05-03 Methode pour l'identification et la representation de differences interindividuelles de sequences d'adn

Country Status (6)

Country Link
EP (1) EP1075550A1 (fr)
JP (1) JP2002513915A (fr)
AU (1) AU4602199A (fr)
CA (1) CA2340623A1 (fr)
DE (1) DE19821114A1 (fr)
WO (1) WO1999057308A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7058517B1 (en) 1999-06-25 2006-06-06 Genaissance Pharmaceuticals, Inc. Methods for obtaining and using haplotype data
US6844154B2 (en) 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
US6931326B1 (en) 2000-06-26 2005-08-16 Genaissance Pharmaceuticals, Inc. Methods for obtaining and using haplotype data
JP2009195226A (ja) * 2008-01-25 2009-09-03 Food & Agricultural Materials Inspection Center 動物由来ｄｎａ検出用プライマー配列

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
WO1996036737A1 (fr) * 1995-05-19 1996-11-21 Ely Michael Rabani Sequençage de polynucleotides parallele et en multiplex

Also Published As

Publication number Publication date
WO1999057308A1 (fr) 1999-11-11
DE19821114A1 (de) 1999-11-11
JP2002513915A (ja) 2002-05-14
EP1075550A1 (fr) 2001-02-14
AU4602199A (en) 1999-11-23

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