CA2339517A1 - Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat - Google Patents
Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat Download PDFInfo
- Publication number
- CA2339517A1 CA2339517A1 CA002339517A CA2339517A CA2339517A1 CA 2339517 A1 CA2339517 A1 CA 2339517A1 CA 002339517 A CA002339517 A CA 002339517A CA 2339517 A CA2339517 A CA 2339517A CA 2339517 A1 CA2339517 A1 CA 2339517A1
- Authority
- CA
- Canada
- Prior art keywords
- ala
- gly
- leu
- val
- ile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
L'invention porte sur des procédés de modification de la spécificité du substrat de la synthase bêta-cétoacyl-ACP, et sur des synthases de bêta-cétoacyl-ACP mises au point. L'invention porte également sur des séquences d'ADN et sur des produits de recombinaison dans l'expression des synthases bêta-cétoacyl-ACP mises au point, ainsi que sur de nouvelles synthases bêta-cétoaxyl-ACP obtenues à partir de ces procédés. Ces séquences d'ADN peuvent être utilisées dans l'expression des synthases bêta-cétoacyl-ACP mises au point des cellules hôtes, notamment des cellules de graines de plantes de récoltes oléagineuses, en vue de modifier la composition des acides gras.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13830899P | 1999-06-09 | 1999-06-09 | |
US60/138,308 | 1999-06-09 | ||
PCT/US2000/016151 WO2000075343A2 (fr) | 1999-06-09 | 2000-06-09 | MISE AU POINT DE LA SYNTHASE DE β-CETOACYL ACP AYANT UNE NOUVELLE SPECIFICITE DE SUBSTRAT |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2339517A1 true CA2339517A1 (fr) | 2000-12-14 |
Family
ID=22481450
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002339517A Abandoned CA2339517A1 (fr) | 1999-06-09 | 2000-06-09 | Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1114163A2 (fr) |
JP (1) | JP2003501063A (fr) |
CA (1) | CA2339517A1 (fr) |
WO (1) | WO2000075343A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR013633A1 (es) | 1997-04-11 | 2001-01-10 | Calgene Llc | METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA. |
WO2002008403A2 (fr) * | 2000-07-25 | 2002-01-31 | Calgene Llc | Sequences d'acide nucleique codantes pour la beta-cetoacyl-acp synthase et utilisation de celles-ci |
US10774349B2 (en) | 2015-05-07 | 2020-09-15 | William Marsh Rice University | Alpha omega bifunctional fatty acids |
JP2016214183A (ja) * | 2015-05-22 | 2016-12-22 | 花王株式会社 | 脂質の製造方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2206984A1 (fr) * | 1995-05-15 | 1996-11-21 | Calgene, Inc. | Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat |
AR013633A1 (es) * | 1997-04-11 | 2001-01-10 | Calgene Llc | METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA. |
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2000
- 2000-06-09 WO PCT/US2000/016151 patent/WO2000075343A2/fr active Application Filing
- 2000-06-09 CA CA002339517A patent/CA2339517A1/fr not_active Abandoned
- 2000-06-09 JP JP2001501626A patent/JP2003501063A/ja active Pending
- 2000-06-09 EP EP00939815A patent/EP1114163A2/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
WO2000075343A2 (fr) | 2000-12-14 |
WO2000075343A3 (fr) | 2001-04-05 |
EP1114163A2 (fr) | 2001-07-11 |
JP2003501063A (ja) | 2003-01-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued | ||
FZDE | Discontinued |
Effective date: 20120611 |