CA2339517A1 - Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat - Google Patents

Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat Download PDF

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Publication number
CA2339517A1
CA2339517A1 CA002339517A CA2339517A CA2339517A1 CA 2339517 A1 CA2339517 A1 CA 2339517A1 CA 002339517 A CA002339517 A CA 002339517A CA 2339517 A CA2339517 A CA 2339517A CA 2339517 A1 CA2339517 A1 CA 2339517A1
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CA
Canada
Prior art keywords
ala
gly
leu
val
ile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002339517A
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English (en)
Inventor
Katayoon Dehesh
Dale Val
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2339517A1 publication Critical patent/CA2339517A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention porte sur des procédés de modification de la spécificité du substrat de la synthase bêta-cétoacyl-ACP, et sur des synthases de bêta-cétoacyl-ACP mises au point. L'invention porte également sur des séquences d'ADN et sur des produits de recombinaison dans l'expression des synthases bêta-cétoacyl-ACP mises au point, ainsi que sur de nouvelles synthases bêta-cétoaxyl-ACP obtenues à partir de ces procédés. Ces séquences d'ADN peuvent être utilisées dans l'expression des synthases bêta-cétoacyl-ACP mises au point des cellules hôtes, notamment des cellules de graines de plantes de récoltes oléagineuses, en vue de modifier la composition des acides gras.
CA002339517A 1999-06-09 2000-06-09 Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat Abandoned CA2339517A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13830899P 1999-06-09 1999-06-09
US60/138,308 1999-06-09
PCT/US2000/016151 WO2000075343A2 (fr) 1999-06-09 2000-06-09 MISE AU POINT DE LA SYNTHASE DE β-CETOACYL ACP AYANT UNE NOUVELLE SPECIFICITE DE SUBSTRAT

Publications (1)

Publication Number Publication Date
CA2339517A1 true CA2339517A1 (fr) 2000-12-14

Family

ID=22481450

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002339517A Abandoned CA2339517A1 (fr) 1999-06-09 2000-06-09 Mise au point de la synthase de .beta.-cetoacyl acp ayant une nouvelle specificite de substrat

Country Status (4)

Country Link
EP (1) EP1114163A2 (fr)
JP (1) JP2003501063A (fr)
CA (1) CA2339517A1 (fr)
WO (1) WO2000075343A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR013633A1 (es) 1997-04-11 2001-01-10 Calgene Llc METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.
CA2412400A1 (fr) 2000-07-25 2002-01-31 Calgene Llc Sequences d'acide nucleique codantes pour la beta-cetoacyl-acp synthase et utilisation de celles-ci
WO2016179572A1 (fr) 2015-05-07 2016-11-10 William Marsh Rice University Acides gras bifonctionnels alpha, oméga
JP2016214183A (ja) * 2015-05-22 2016-12-22 花王株式会社 脂質の製造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11505115A (ja) * 1995-05-15 1999-05-18 カルジーン，インコーポレーテッド 植物チオエステラーゼの遺伝子操作および新規な基質特異性を有する植物チオエステラーゼの開示
AR013633A1 (es) * 1997-04-11 2001-01-10 Calgene Llc METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.

Also Published As

Publication number Publication date
JP2003501063A (ja) 2003-01-14
EP1114163A2 (fr) 2001-07-11
WO2000075343A2 (fr) 2000-12-14
WO2000075343A3 (fr) 2001-04-05

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued
FZDE Discontinued

Effective date: 20120611