CA2379813A1 - Sequences d'acides nucleiques codant pour des acides gras polyenoiques isomerase et leurs utilisations - Google Patents

Sequences d'acides nucleiques codant pour des acides gras polyenoiques isomerase et leurs utilisations Download PDF

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Publication number
CA2379813A1
CA2379813A1 CA002379813A CA2379813A CA2379813A1 CA 2379813 A1 CA2379813 A1 CA 2379813A1 CA 002379813 A CA002379813 A CA 002379813A CA 2379813 A CA2379813 A CA 2379813A CA 2379813 A1 CA2379813 A1 CA 2379813A1
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CA
Canada
Prior art keywords
sequence
seq
plant
polynucleotide
organism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379813A
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English (en)
Inventor
Wei Zheng
Ling Yuan
James George Metz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Monsanto Technology LLC
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Filing date
Publication date
Application filed by Monsanto Technology LLC filed Critical Monsanto Technology LLC
Publication of CA2379813A1 publication Critical patent/CA2379813A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des séquences d'acides nucléiques ainsi que des procédés permettant de produire des acides gras conjugués dans des cellules hôtes. Les séquences d'acides nucléiques sont obtenues à partir d'algues qui codent pour des d'acides gras polyénoïques isomérase. Lesdites séquences peuvent servir dans des constructions d'expression pour diriger l'expression des séquences PFI dans des cellules hôtes. L'expression de PFI dans des cellules hôtes transgéniques permet de produire des acides gras conjugués dans la cellule hôte.
CA002379813A 1999-07-30 2000-07-28 Sequences d'acides nucleiques codant pour des acides gras polyenoiques isomerase et leurs utilisations Abandoned CA2379813A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14645899P 1999-07-30 1999-07-30
US60/146,458 1999-07-30
PCT/US2000/020597 WO2001009296A1 (fr) 1999-07-30 2000-07-28 Sequences d'acides nucleiques codant pour des acides gras polyenoiques isomerase et leurs utilisations

Publications (1)

Publication Number Publication Date
CA2379813A1 true CA2379813A1 (fr) 2001-02-08

Family

ID=22517454

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379813A Abandoned CA2379813A1 (fr) 1999-07-30 2000-07-28 Sequences d'acides nucleiques codant pour des acides gras polyenoiques isomerase et leurs utilisations

Country Status (6)

Country Link
EP (1) EP1218497A1 (fr)
AU (1) AU6749400A (fr)
BR (1) BR0012873A (fr)
CA (1) CA2379813A1 (fr)
MX (1) MXPA02001086A (fr)
WO (1) WO2001009296A1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856149A (en) * 1995-06-01 1999-01-05 Wisconsin Alumni Research Foundation Method of producing conjugated fatty acids
JP2002508929A (ja) * 1997-12-23 2002-03-26 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ リノール酸イソメラーゼ
JPH11253162A (ja) * 1998-03-10 1999-09-21 Mitsubishi Chemical Corp モノアミンオキシダーゼをコードするｄｎａ

Also Published As

Publication number Publication date
BR0012873A (pt) 2002-04-16
WO2001009296A1 (fr) 2001-02-08
AU6749400A (en) 2001-02-19
MXPA02001086A (es) 2002-08-12
EP1218497A1 (fr) 2002-07-03

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20040420