CA2324561A1 - Withering-preventing and quick-acting nutrition supplementing agent for gramineous plants - Google Patents
Withering-preventing and quick-acting nutrition supplementing agent for gramineous plants Download PDFInfo
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- CA2324561A1 CA2324561A1 CA002324561A CA2324561A CA2324561A1 CA 2324561 A1 CA2324561 A1 CA 2324561A1 CA 002324561 A CA002324561 A CA 002324561A CA 2324561 A CA2324561 A CA 2324561A CA 2324561 A1 CA2324561 A1 CA 2324561A1
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- withering
- quick
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- proline
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
- Cultivation Of Plants (AREA)
Abstract
A withering-preventing and quick-acting nutrition supplementing agent for plants, particularly gramineous plants, comprises proline and optionally inosine, together with a carrier therefor. Such an agent is not a chemical fertilizer and does not adversely affect the environment as well as humans and animals.
Description
WITHERING-PREVENTING AND QUICK-ACTING NUTRITION
SUPPLEMENTING AGENT FOR GRAMINEOUS PLANTS
The present invention relates to a withering-preventing and quick-acting s nutrition supplementing agent for plants such as gramineous plants. More particularly, the invention is directed to a withering-preventing and quick-acting nutrition supplementing agent comprising proline which is effective for, e.g., lawn grass going to wither and die by stress caused by temperature, pruning or leaf rot disease.
to It is known to apply an amino acid-related compound, e.g., proline, to a plant. For example, Laid-Open (Kokai) Japanese Patent Application No.
67051/1973 discloses a fruiting and fruit-enlarging promoting agent containing at least one nucleoside or nucleotide and proline. In respect to this fruiting and fruit-enlarging promoting agent, however, the target plants are entirely is different from those plants to be treated with the withering-preventing and quick-acting nutrition supplementing agent according to the present invention, and the promoting agent necessitates concurrent use of a nucleoside or a nucleotide in combination with proline. In addition, the application method comprises performing foliar application (e.g., spraying or spreading the 2o promoting agent onto the leaves) at intervals of one week by injecting dropwise the fruiting and fruit-enlarging promoting agent at a concentration of 40 ppm with a syringe for two months.
Laid-Open (Kokai) Japanese Patent Application No. 279405/1998 discloses a method for controlling pine tree withering, wherein a plant 2s activating substance containing proline as the main ingredient and an alkaline water are sprayed onto the above-ground parts of a pine tree, and/or such a plant activating substance and an acidic water are fed to the subterranean parts of the pine tree. In this method, the target plants are also different from the plants to be treated with the withering-prevention and quick-acting nutrition 3o supplementing agent according to the present invention. In addition, according to the prior art method, a solution having a proline concentration of 30 to ppm is used.
Lawns are utilized in many places such as parks, gardens and ball game fields, and are indispensable for golf courses or golf links. However, a large s quantity of fertilizer and agricultural chemicals is used for maintenance of the lawn grass, causing a problem in respect of the environment. For example, synthetic fungicides have hitherto been applied for controlling leaf rot disease of lawn grass, but such agricultural chemicals may adversely affect the natural environment.
o Furthermore, lawn grass, especially cold-district type grass, suffers indirectly growth stop and directly withering due to stress caused by high temperatures, and the only measure against such a problem is good air-ventilation.
As for gramineous plants such as lawn grass, it is known to fertilize Is these plants with amino acids as a nitrogen source, but the amino acids are generally used in the form of a multi-component fertilizer such as a hydrolysate of plants or animals or a fermentation waste liquid, the components being indistinguishable. Since fertilization must be reduced in view of the problems associated with the accumulation of minerals in the soil, there is a demand for a 2o quick=acting fertilizer or quick-acting nutrition supplementing agent which can effectively supply the necessary amount of nitrogen. For this purpose, urea preparations for foliar application are frequently used, but the urea tends to accumulate in the soil and may cause phenomena such as leaf scorch and the like in some cases depending on the concentration.
2s It is therefore an object of the present invention to overcome the above drawbacks and to provide a withering-preventing and quick-acting nutrition supplementing agent which is not a chemical fertilizer and does not adversely affect the environment as well as humans and animals.
As a result of extensive studies for achieving the aforementioned 30 objects, Applicant has found that amino acids, especially proline, serine and the like, exhibit a remarkable effect on the prevention of withering of gramineous plants, and that the effect is especially enhanced by combining such an amino acid with inosine among various nucleosides.
According to one aspect of the invention, there is provided a withering-s preventing and quick-acting nutrition supplementing agent for gramineous plants, comprising as active ingredient proline together with a carrier therefor.
According to another aspect of the invention, there is provided a withering-preventing and quick-acting nutrition supplementing agent for plants, particularly gramineous plants, comprising at least one amino acid and inosine, t o together with a carrier therefor.
The present invention also provides, in a further aspect thereof, a method of preventing withering and supplementing quick-acting nutrition of plants, particularly gramineous plants, comprising applying a withering-preventing and quick-acting nutrition supplementing agent as defined above to is the soil or hydroponic water, or performing foliar application of such an agent.
Gramineous plants, especially lawn grasses, rice plants and pasture plants are included in the target plants to which the withering-preventing and quick-acting nutrition supplementing agent of the present invention is applied, and the agent is particularly effective for lawn grasses.
2o The proline used does require to be a purified product and can be a hydrolysate of a protein or a mixture of amino acids containing a large quantity of proline, unless it exerts an adverse effect such as salt damage, leaf scorch or the like. In the case where proline is added to hydroponic water, however, in order to prevent contamination and rot of the hydroponic water, the proline is 2s preferably in a form free from impurities which cause such contamination and rot.
Where the withering-preventing and quick-acting nutrition supplementing agent according to the present invention, containing proline as active ingredient, is applied to the soil or hydroponic water, the carrier used is 3o preferably a solvent such as water. In the case of foliar application, a liquid preparation having a proline concentration of 0.2 to 29 ppm is preferably used.
Moreover, the agent can be formulated into powder, granules or tablets by using an appropriate filler, binder or the like. Where use is made of a solvent, a fungicide, a surfactant or a preservative can be added to the agent for s preventing rot. Furthermore, in the case of foliar application, concurrent use with a spreading/adhering agent is effective.
With regard to the suitable time for applying the withering-preventing and quick-acting nutrition supplementing agent of the present invention, in the case of lawn grasses, pasture plants and the like, the agent can be applied as an to additional fertilizer, or applied after mowing grass or at the beginning of withering, for example.
Examples of suitable application methods include foliar application, soil application and addition to hydroponic water. It is preferable to apply proline to the above-ground parts so as to be absorbed from the leaves of a plant such as ~s lawn grass, and inosine to the soil so as to be absorbed from the roots. In this case, the withering-preventing and quick-acting nutrition supplementing agent for lawn grass is not in a form containing both proline and inosine together, but proline and inosine are each applied separately at not too long interval to the above-ground parts and to the soil, respectively.
2o As mentioned above, with regard to inosine, Applicant has found that the combination of inosine and proline is remarkably effective as compared with other combinations of proline and a nucleoside other than inosine, such as the combination of uridine and proline, the combination of adenosine and proline, and the like.
2s Foliar application of proline not only prevents withering of plants such as gramineous plants, but also is effective as a means of feeding nitrogen immediately and promoting growth.
The application rate or amount varies depending on the time of application, the kind of gramineous plants (lawn grasses, rice plants, pasture 3o plants, and the like), the cultivation density, the growing stage, etc. In short, the rate of application can be determined as being those values at which the prevention of withering and the growth of, for instance, a lawn grass cultivated by using the withering-preventing and quick-acting nutrition supplementing agent of the present invention are superior to those of the lawn grass cultivated s under entirely the same conditions, with the exception that the withering-preventing and quick-acting nutrition supplementing agent of the present invention has not been applied. It is possible to determine these values by preliminary comparative testing which one skilled in the art can easily carry out. As mentioned above, in the case of foliar application, a liquid preparation io having a proline concentration of 0.2 to 29 ppm is particularly effective and such a low concentration can be employed. Thus, prevention of withering and promotion of growth of a gramineous plant can be effected at such a low concentration.
In addition, the application rate of inosine can be from 0.05 to 1 ppm (5 s to 100 g per 100 tons of soil) in the case of soil application, for example.
In the case of hydroponic cultivation, inosine can be added in an amount of 0.1 to 5 ppm to the hydroponic water.
The following non-limiting example further illustrates the invention, reference being made to the accompanying drawings in which:
2o Figure 1 shows the state of the pot cultivation of lawn grass plants on the 35th day from transplantation; and Figure 2 shows the state of the grass plants after having been washed with water on the 35th day from the transplantation.
Example 1: hydro~onic cultivation of a lawn grass 2s Seedlings of a lawn grass (European grass, evergreen lawn grass) were cultivated and divided into eight groups A to H, each group being composed of 80 stocks. Then, they were cultivated hydroponically under the conditions shown in the accompanying Table 1. In groups B to H, inosine and uridine were added to the hydroponic liquid in amounts such as to provide the 3o concentrations shown in Table l; proline was diluted with water in an amount such as to provide the concentrations shown in the table and sprayed onto the leaves every day. Group A was used as a control group.
On the 35th day, five average stocks which had not been withered were sampled from each group. As shown in Table 1, at the root, leaf length and total weight of the living plants, better growth was found in the proline-treated plants, especially at a concentration of 2 ppm. Furthermore, it was confirmed that the effect became more remarkable when 2 ppm of inosine were used in combination with proline. In the Table, the number of days till the plants were withered represents the number of days from transplantation of the seedlings i o till about 70 percent of the leaves were withered.
Photographs of the state of the pot cultivations of groups A to D on the 35th day from the transplantation of the lawn grass plants are shown in Figure 1. Moreover, Figure 2 shows photographs of the state of every five stocks of the same four groups on the 35th day from the transplantation of the grass is plants. From these photographs, better growth was also found in the proline-treated plants, especially at a concentration of 2 ppm, and it was confirmed that the effect became more remarkable when 2 ppm of inosine was used in combination with proline.
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SUPPLEMENTING AGENT FOR GRAMINEOUS PLANTS
The present invention relates to a withering-preventing and quick-acting s nutrition supplementing agent for plants such as gramineous plants. More particularly, the invention is directed to a withering-preventing and quick-acting nutrition supplementing agent comprising proline which is effective for, e.g., lawn grass going to wither and die by stress caused by temperature, pruning or leaf rot disease.
to It is known to apply an amino acid-related compound, e.g., proline, to a plant. For example, Laid-Open (Kokai) Japanese Patent Application No.
67051/1973 discloses a fruiting and fruit-enlarging promoting agent containing at least one nucleoside or nucleotide and proline. In respect to this fruiting and fruit-enlarging promoting agent, however, the target plants are entirely is different from those plants to be treated with the withering-preventing and quick-acting nutrition supplementing agent according to the present invention, and the promoting agent necessitates concurrent use of a nucleoside or a nucleotide in combination with proline. In addition, the application method comprises performing foliar application (e.g., spraying or spreading the 2o promoting agent onto the leaves) at intervals of one week by injecting dropwise the fruiting and fruit-enlarging promoting agent at a concentration of 40 ppm with a syringe for two months.
Laid-Open (Kokai) Japanese Patent Application No. 279405/1998 discloses a method for controlling pine tree withering, wherein a plant 2s activating substance containing proline as the main ingredient and an alkaline water are sprayed onto the above-ground parts of a pine tree, and/or such a plant activating substance and an acidic water are fed to the subterranean parts of the pine tree. In this method, the target plants are also different from the plants to be treated with the withering-prevention and quick-acting nutrition 3o supplementing agent according to the present invention. In addition, according to the prior art method, a solution having a proline concentration of 30 to ppm is used.
Lawns are utilized in many places such as parks, gardens and ball game fields, and are indispensable for golf courses or golf links. However, a large s quantity of fertilizer and agricultural chemicals is used for maintenance of the lawn grass, causing a problem in respect of the environment. For example, synthetic fungicides have hitherto been applied for controlling leaf rot disease of lawn grass, but such agricultural chemicals may adversely affect the natural environment.
o Furthermore, lawn grass, especially cold-district type grass, suffers indirectly growth stop and directly withering due to stress caused by high temperatures, and the only measure against such a problem is good air-ventilation.
As for gramineous plants such as lawn grass, it is known to fertilize Is these plants with amino acids as a nitrogen source, but the amino acids are generally used in the form of a multi-component fertilizer such as a hydrolysate of plants or animals or a fermentation waste liquid, the components being indistinguishable. Since fertilization must be reduced in view of the problems associated with the accumulation of minerals in the soil, there is a demand for a 2o quick=acting fertilizer or quick-acting nutrition supplementing agent which can effectively supply the necessary amount of nitrogen. For this purpose, urea preparations for foliar application are frequently used, but the urea tends to accumulate in the soil and may cause phenomena such as leaf scorch and the like in some cases depending on the concentration.
2s It is therefore an object of the present invention to overcome the above drawbacks and to provide a withering-preventing and quick-acting nutrition supplementing agent which is not a chemical fertilizer and does not adversely affect the environment as well as humans and animals.
As a result of extensive studies for achieving the aforementioned 30 objects, Applicant has found that amino acids, especially proline, serine and the like, exhibit a remarkable effect on the prevention of withering of gramineous plants, and that the effect is especially enhanced by combining such an amino acid with inosine among various nucleosides.
According to one aspect of the invention, there is provided a withering-s preventing and quick-acting nutrition supplementing agent for gramineous plants, comprising as active ingredient proline together with a carrier therefor.
According to another aspect of the invention, there is provided a withering-preventing and quick-acting nutrition supplementing agent for plants, particularly gramineous plants, comprising at least one amino acid and inosine, t o together with a carrier therefor.
The present invention also provides, in a further aspect thereof, a method of preventing withering and supplementing quick-acting nutrition of plants, particularly gramineous plants, comprising applying a withering-preventing and quick-acting nutrition supplementing agent as defined above to is the soil or hydroponic water, or performing foliar application of such an agent.
Gramineous plants, especially lawn grasses, rice plants and pasture plants are included in the target plants to which the withering-preventing and quick-acting nutrition supplementing agent of the present invention is applied, and the agent is particularly effective for lawn grasses.
2o The proline used does require to be a purified product and can be a hydrolysate of a protein or a mixture of amino acids containing a large quantity of proline, unless it exerts an adverse effect such as salt damage, leaf scorch or the like. In the case where proline is added to hydroponic water, however, in order to prevent contamination and rot of the hydroponic water, the proline is 2s preferably in a form free from impurities which cause such contamination and rot.
Where the withering-preventing and quick-acting nutrition supplementing agent according to the present invention, containing proline as active ingredient, is applied to the soil or hydroponic water, the carrier used is 3o preferably a solvent such as water. In the case of foliar application, a liquid preparation having a proline concentration of 0.2 to 29 ppm is preferably used.
Moreover, the agent can be formulated into powder, granules or tablets by using an appropriate filler, binder or the like. Where use is made of a solvent, a fungicide, a surfactant or a preservative can be added to the agent for s preventing rot. Furthermore, in the case of foliar application, concurrent use with a spreading/adhering agent is effective.
With regard to the suitable time for applying the withering-preventing and quick-acting nutrition supplementing agent of the present invention, in the case of lawn grasses, pasture plants and the like, the agent can be applied as an to additional fertilizer, or applied after mowing grass or at the beginning of withering, for example.
Examples of suitable application methods include foliar application, soil application and addition to hydroponic water. It is preferable to apply proline to the above-ground parts so as to be absorbed from the leaves of a plant such as ~s lawn grass, and inosine to the soil so as to be absorbed from the roots. In this case, the withering-preventing and quick-acting nutrition supplementing agent for lawn grass is not in a form containing both proline and inosine together, but proline and inosine are each applied separately at not too long interval to the above-ground parts and to the soil, respectively.
2o As mentioned above, with regard to inosine, Applicant has found that the combination of inosine and proline is remarkably effective as compared with other combinations of proline and a nucleoside other than inosine, such as the combination of uridine and proline, the combination of adenosine and proline, and the like.
2s Foliar application of proline not only prevents withering of plants such as gramineous plants, but also is effective as a means of feeding nitrogen immediately and promoting growth.
The application rate or amount varies depending on the time of application, the kind of gramineous plants (lawn grasses, rice plants, pasture 3o plants, and the like), the cultivation density, the growing stage, etc. In short, the rate of application can be determined as being those values at which the prevention of withering and the growth of, for instance, a lawn grass cultivated by using the withering-preventing and quick-acting nutrition supplementing agent of the present invention are superior to those of the lawn grass cultivated s under entirely the same conditions, with the exception that the withering-preventing and quick-acting nutrition supplementing agent of the present invention has not been applied. It is possible to determine these values by preliminary comparative testing which one skilled in the art can easily carry out. As mentioned above, in the case of foliar application, a liquid preparation io having a proline concentration of 0.2 to 29 ppm is particularly effective and such a low concentration can be employed. Thus, prevention of withering and promotion of growth of a gramineous plant can be effected at such a low concentration.
In addition, the application rate of inosine can be from 0.05 to 1 ppm (5 s to 100 g per 100 tons of soil) in the case of soil application, for example.
In the case of hydroponic cultivation, inosine can be added in an amount of 0.1 to 5 ppm to the hydroponic water.
The following non-limiting example further illustrates the invention, reference being made to the accompanying drawings in which:
2o Figure 1 shows the state of the pot cultivation of lawn grass plants on the 35th day from transplantation; and Figure 2 shows the state of the grass plants after having been washed with water on the 35th day from the transplantation.
Example 1: hydro~onic cultivation of a lawn grass 2s Seedlings of a lawn grass (European grass, evergreen lawn grass) were cultivated and divided into eight groups A to H, each group being composed of 80 stocks. Then, they were cultivated hydroponically under the conditions shown in the accompanying Table 1. In groups B to H, inosine and uridine were added to the hydroponic liquid in amounts such as to provide the 3o concentrations shown in Table l; proline was diluted with water in an amount such as to provide the concentrations shown in the table and sprayed onto the leaves every day. Group A was used as a control group.
On the 35th day, five average stocks which had not been withered were sampled from each group. As shown in Table 1, at the root, leaf length and total weight of the living plants, better growth was found in the proline-treated plants, especially at a concentration of 2 ppm. Furthermore, it was confirmed that the effect became more remarkable when 2 ppm of inosine were used in combination with proline. In the Table, the number of days till the plants were withered represents the number of days from transplantation of the seedlings i o till about 70 percent of the leaves were withered.
Photographs of the state of the pot cultivations of groups A to D on the 35th day from the transplantation of the lawn grass plants are shown in Figure 1. Moreover, Figure 2 shows photographs of the state of every five stocks of the same four groups on the 35th day from the transplantation of the grass is plants. From these photographs, better growth was also found in the proline-treated plants, especially at a concentration of 2 ppm, and it was confirmed that the effect became more remarkable when 2 ppm of inosine was used in combination with proline.
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Claims (12)
1. A withering-preventing and quick-acting nutrition supplementing agent for gramineous plants, comprising as active ingredient proline together with a carrier therefor.
2. A withering-preventing and quick-acting nutrition supplementing agent according to claim 1, wherein the carrier is a solvent and proline is present in a concentration of 0.2 to 29 ppm.
3. A withering-preventing and quick-acting nutrition supplementing agent according to claim 2, wherein the solvent is water.
4. The withering-preventing and quick-acting nutrition supplementing agent for plants, comprising at least one amino acid and inosine, together with a carrier therefor.
5. A withering-preventing and quick-acting nutrition supplementing agent for gramineous plants comprising at least one amino acid and inosine, together with a carrier therefor.
6. A withering-preventing and quick-acting nutrition supplementing agent according to claim 4 or 5, wherein the amino acid is proline.
7. A withering-preventing and quick-acting nutrition supplementing agent according to claim 4 or 6, wherein the carrier is a solvent and proline is present in a concentration of 0.2 to 29 ppm.
8. A withering-preventing and quick-acting nutrition supplementing agent according to claim 7, for soil application, wherein inosine is present in a concentration to provide an application rate of 0.05 to 1 ppm.
9. A withering-preventing and quick-acting nutrition supplementing agent according to claim 7, for hydroponic cultivation, wherein inosine is present in a concentration to provide an application rate of 0.1 to 5 ppm.
10. A method of preventing withering and supplementing quick-acting nutrition of plants, which comprises performing foliar application of a withering-preventing and quick-acting nutrition supplementing agent as defined in any one of claims 1 to 7.
11. A method of preventing withering and supplementing quick-acting nutrition of plants, which comprises treating the plants with proline and inosine.
12. A method of preventing withering and supplementing quick-acting nutrition of lawn grass, which comprises applying proline to above-ground parts of the lawn grass, and thereafter applying inosine to soil.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP30828199A JP2001131009A (en) | 1999-10-29 | 1999-10-29 | Withering-preventing and quickly-acting nutritional supplement agent for gramineous plant |
JP308,281/99 | 1999-10-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2324561A1 true CA2324561A1 (en) | 2001-04-29 |
Family
ID=17979154
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002324561A Abandoned CA2324561A1 (en) | 1999-10-29 | 2000-10-26 | Withering-preventing and quick-acting nutrition supplementing agent for gramineous plants |
Country Status (7)
Country | Link |
---|---|
US (1) | US6448202B1 (en) |
EP (1) | EP1095565B1 (en) |
JP (1) | JP2001131009A (en) |
KR (1) | KR20010051171A (en) |
CN (1) | CN1294101A (en) |
CA (1) | CA2324561A1 (en) |
DE (1) | DE60011366T2 (en) |
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US20050112177A1 (en) * | 2002-12-20 | 2005-05-26 | Dopson Minter H. | Nutritional supplement composition and method |
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US4520103A (en) * | 1982-10-27 | 1985-05-28 | Amgen | Microbial production of indigo |
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EP0512066B1 (en) * | 1990-01-22 | 1996-11-13 | THE UNITED STATES OF AMERICA as represented by the Secretary UNITED STATES DEPARTMENT OF COMMERCE | Co-independent growth medium for maintenance and propagation of cells |
US5780709A (en) | 1993-08-25 | 1998-07-14 | Dekalb Genetics Corporation | Transgenic maize with increased mannitol content |
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JPH10279405A (en) | 1997-04-03 | 1998-10-20 | Mitsui Chem Inc | Method of preventing pine-withering |
-
1999
- 1999-10-29 JP JP30828199A patent/JP2001131009A/en active Pending
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2000
- 2000-10-20 KR KR1020000061929A patent/KR20010051171A/en not_active Application Discontinuation
- 2000-10-23 CN CN00131490A patent/CN1294101A/en active Pending
- 2000-10-26 US US09/695,993 patent/US6448202B1/en not_active Expired - Fee Related
- 2000-10-26 CA CA002324561A patent/CA2324561A1/en not_active Abandoned
- 2000-10-26 EP EP00309453A patent/EP1095565B1/en not_active Expired - Lifetime
- 2000-10-26 DE DE60011366T patent/DE60011366T2/en not_active Expired - Fee Related
Cited By (2)
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CN103238393A (en) * | 2012-06-14 | 2013-08-14 | 西南大学 | Seed soaking method with penicillin |
CN103238393B (en) * | 2012-06-14 | 2014-10-15 | 西南大学 | Seed soaking method with penicillin |
Also Published As
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US6448202B1 (en) | 2002-09-10 |
CN1294101A (en) | 2001-05-09 |
DE60011366T2 (en) | 2005-06-23 |
JP2001131009A (en) | 2001-05-15 |
KR20010051171A (en) | 2001-06-25 |
DE60011366D1 (en) | 2004-07-15 |
EP1095565A1 (en) | 2001-05-02 |
EP1095565B1 (en) | 2004-06-09 |
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