CA2323719A1 - Structured surfaces with properties which inhibit cell adhesion and cell proliferation - Google Patents

Structured surfaces with properties which inhibit cell adhesion and cell proliferation Download PDF

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Publication number
CA2323719A1
CA2323719A1 CA 2323719 CA2323719A CA2323719A1 CA 2323719 A1 CA2323719 A1 CA 2323719A1 CA 2323719 CA2323719 CA 2323719 CA 2323719 A CA2323719 A CA 2323719A CA 2323719 A1 CA2323719 A1 CA 2323719A1
Authority
CA
Canada
Prior art keywords
elevations
structured surface
structured
unstructured
article according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2323719
Other languages
French (fr)
Inventor
Markus Oles
Bernhard Schleich
Peter Hommes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Creavis Gesellschaft fuer Technologie und Innovation mbH
Original Assignee
Creavis Gesellschaft fuer Technologie und Innovation mbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Creavis Gesellschaft fuer Technologie und Innovation mbH filed Critical Creavis Gesellschaft fuer Technologie und Innovation mbH
Publication of CA2323719A1 publication Critical patent/CA2323719A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/005—Surface shaping of articles, e.g. embossing; Apparatus therefor characterised by the choice of material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/02—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing
    • B29C59/022—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing characterised by the disposition or the configuration, e.g. dimensions, of the embossments or the shaping tools therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/02—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing
    • B29C59/022—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing characterised by the disposition or the configuration, e.g. dimensions, of the embossments or the shaping tools therefor
    • B29C2059/023—Microembossing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29K—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES B29B, B29C OR B29D, RELATING TO MOULDING MATERIALS OR TO MATERIALS FOR MOULDS, REINFORCEMENTS, FILLERS OR PREFORMED PARTS, e.g. INSERTS
    • B29K2995/00—Properties of moulding materials, reinforcements, fillers, preformed parts or moulds
    • B29K2995/0037—Other properties
    • B29K2995/0093—Other properties hydrophobic

Abstract

Disclosed are articles having structured (i.e., rough) surfaces which inhibit proliferation and adhesion of eukaryotic cells such as fibroblasts. The structured surfaces have elevations with an average height of from 50 nm to 10 µm and an average separation of from 50 nm to 10 µm, and are made of materials having a surface energy of at least 20 mN/m as measured in an unstructured (i.e., smooth) form. The structured surfaces may be produced by a variety of processes such as an embossing process and the articles may be used e.g.
as cell-culture vessels.

Claims (32)

1. An article having a structured surface which is capable of inhibiting proliferation and adhesion of eukaryotic non-microorganism cells thereon, wherein the surface has protrusions with an average height of from 50 nm to 10 µm and an average separation of from 10 nm to 50 µm and the surface is made of a material which, as measured in its smooth form, has a surface energy of at least 20 mN/m.
2. The article according to claim 1, wherein the protrusions have a ratio of height to width (aspect ratio) of from 0.5 to 20.
3. The article according to claim 2, wherein the ratio of height to width is 1 to 5.
4. The article according to claim 1, 2 or 3, wherein the material of which the structured surface is made has, as measured in its smooth form, a surface energy of 20 to 60 mN/m.
5. The article according to claim 4, wherein the material of which the structured surface is made is an organic polymer material.
6. The article according to claim 5, wherein the organic polymer material is polycarbonate.
7. The article according to any one of claims 1 to 6, which also has a smooth surface.
8. The article according to claim 7, wherein the smooth surface has a surface energy of from 10 to 20 mN/m.
9. The article according to any one of claims 1 to 8, wherein the protrusions are formed on a coarser overstructure of the surface, the overstructure having an average height of from 10 µm to 1 mm and an average separation of from 10 µm to 1 mm.
10. The article according to any one of claims 1 to 9, which is a cell culture vessel for culturing a mammalian cell.
11. A structured surface with properties which inhibit cell proliferation, wherein the surface has elevations with an average height of from 50 nm to µm and with an average separation of from 50 nm to 10 µm, and the surface energy of the unstructured material is above 20 mN/m.
12. The structured surface as claimed in claim 11, wherein the elevations have an average height of from 50 nm to 4 µm.
13. The structured surface as claimed in claim 11, wherein the average separation of the elevations is from 50 nm to 4 µm.
14. The structured surface as claimed in claim 11, wherein the elevations have an average height of from 50 nm to 4 µm and an average separation of from 50 nm to 4 µm.
15. The structured surface as claimed in any of claims 11 to 14, wherein the elevations have an aspect ratio of from 0.5 to 20.
16. The structured surface as claimed in claim 15, wherein the elevations have an aspect ratio of from 1 to 10.
17. The structured surface as claimed in claim 15, wherein the elevations have an aspect ratio of from 1 to 5.
18. The structured surface as claimed in any of claims 11 to 17, wherein the elevations have been applied to an overstructure with an average height of from 10 µm to 1 mm and with an average separation of from 10 µm to 1 mm.
19. The structured surface as claimed in any of claims 11 to 18, wherein the surface also has unstructured portions.
20. The structured surface as claimed in claim 19, wherein the unstructured portions have a surface energy of from 10 to 20 mN/m.
21. The structured surface as claimed in any of claims 11 to 20, wherein the unstructured material comprises silicones, polydioxanes, fibronectin, collagen, fibrin, polyurethanes, polymethyl methacrylate, polyacrylic acid, polyvinyl chloride, polyethylene, polypropylene, polyimides or polyamides in the form of homo- or copolymer.
22. The structured surface as claimed in any of claims 11 to 20, wherein the unstructured material is composed of gold, titanium, quartz glass, lithium niobate, silicon carbide, silicon nitride, hydroxyapatite or silicon.
23. A process for producing structured surfaces with properties which inhibit cell proliferation, which comprises using mechanical embossing or lithographic etching processes or a molding process to apply elevations with an average height of from 50 nm to 10 µm and with an average separation of from 50 nm to µm onto an unstructured material with a surface energy above mN/m.
24. The process as claimed in claim 23 , wherein the elevations have been applied, simultaneously with the overstructure or subsequent thereto, using mechanical embossing, lithographic etching processes or a molding process on an overstructure with an average height of from 10 µm to 1 mm and with an average separation of from 10 µm to 1 mm.
25. The process as claimed in claim 23 or 24, wherein the elevations have subsequently been provided with a material with a surface energy above 20 mN/m.
26. The process as claimed in any of claims 23 to 25, wherein the unstructured material comprises silicones, polydioxanes, fibronectin, collagen, fibrin, polyurethanes, polymethyl methacrylate, polyacrylic acid, polyvinyl chloride, polyethylene, polypropylene, polyimides or polyamides in the form of homo- or copolymer.
27. The process as claimed in any of claims 23 to 25, wherein the unstructured material is composed of gold, titanium, quartz glass, lithium niobate, silicon carbide, silicon nitride, hydroxyapatite or silicon.
28. The process as claimed in any of claims 23 to 27, wherein the unstructured or structured material is subjected to a plasma treatment.
29. The process as claimed in any of claims 23 to 28, wherein mechanical or lithographic methods are used to give the structured surface unstructured portions.
30. The use of the structured surfaces with properties which inhibit cell proliferation, as claimed in any of claims 11 to 22, for cell-culture vessels or as a bioassay.
31. The use of the structured surface with properties which inhibit cell proliferation, as claimed in any of claims11to22, in cell screening or active substance screening, in medicine, in crop protection or in toxicology.
32. The use of the structured surface with properties which inhibit cell proliferation, as claimed in any of claims 11 to 22, for producing medical implants.
CA 2323719 1999-10-20 2000-10-18 Structured surfaces with properties which inhibit cell adhesion and cell proliferation Abandoned CA2323719A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999150452 DE19950452A1 (en) 1999-10-20 1999-10-20 Structured surfaces with cell adhesion and cell proliferation inhibiting properties
DE19950452.0 1999-10-20

Publications (1)

Publication Number Publication Date
CA2323719A1 true CA2323719A1 (en) 2001-04-20

Family

ID=7926233

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2323719 Abandoned CA2323719A1 (en) 1999-10-20 2000-10-18 Structured surfaces with properties which inhibit cell adhesion and cell proliferation

Country Status (4)

Country Link
EP (1) EP1095760A1 (en)
JP (1) JP2001149061A (en)
CA (1) CA2323719A1 (en)
DE (1) DE19950452A1 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7195872B2 (en) 2001-11-09 2007-03-27 3D Biosurfaces, Inc. High surface area substrates for microarrays and methods to make same
US7374903B2 (en) 2001-06-13 2008-05-20 The University Of Liverpool Substrates with topographical features for the manipulation of cellular behavior and response
US20120141743A1 (en) * 2009-03-16 2012-06-07 Malgorzata-Jadwiga Kolodziej Biocide coating comprising copper
US9085019B2 (en) 2010-10-28 2015-07-21 3M Innovative Properties Company Superhydrophobic films
EP2979844A4 (en) * 2014-04-22 2016-03-23 Sharp Kk Synthetic polymer membrane having surface with sterilizing activity, laminate equipped with synthetic polymer membrane, sterilization method utilizing surface of synthetic polymer membrane, method for reactivating surface of synthetic polymer membrane, mold for use in production of synthetic polymer membrane, and method for producing mold
US9650661B2 (en) 2013-05-21 2017-05-16 3M Innovative Properties Company Nanostructured spore carrier
US10934405B2 (en) 2018-03-15 2021-03-02 Sharp Kabushiki Kaisha Synthetic polymer film whose surface has microbicidal activity, plastic product which includes synthetic polymer film, sterilization method with use of surface of synthetic polymer film, photocurable resin composition, and manufacturing method of synthetic polymer film
US10968292B2 (en) 2017-09-26 2021-04-06 Sharp Kabushiki Kaisha Synthetic polymer film whose surface has microbicidal activity, photocurable resin composition, manufacturing method of synthetic polymer film, and sterilization method with use of surface of synthetic polymer film
US11364673B2 (en) 2018-02-21 2022-06-21 Sharp Kabushiki Kaisha Synthetic polymer film and production method of synthetic polymer film

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DE10146027B4 (en) * 2001-09-18 2006-07-13 Huppmann Ag Component for a brewery plant and method for producing such components
DE10210027A1 (en) 2002-03-07 2003-09-18 Creavis Tech & Innovation Gmbh Hydrophilic surfaces
WO2005051450A1 (en) * 2003-11-28 2005-06-09 Zeon Medical, Inc. Cell growth-inhibiting film, medical instrument and stent for digestive organs
JP4512351B2 (en) * 2003-11-28 2010-07-28 ゼオンメディカル株式会社 Gastrointestinal stent
JP4610885B2 (en) * 2003-11-28 2011-01-12 ゼオンメディカル株式会社 Cell growth suppression film and medical device
DE102008060991A1 (en) * 2008-12-08 2010-06-10 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Subtrate for selection and specific influence on the function of cells
US20130211310A1 (en) * 2010-10-28 2013-08-15 3M Innovative Properties Company Engineered surfaces for reducing bacterial adhesion
WO2013003373A1 (en) * 2011-06-27 2013-01-03 3M Innovative Properties Company Microstructured surfaces for reducing bacterial adhesion
DE102013111759A1 (en) * 2013-10-25 2015-04-30 Bürkert Werke GmbH Apparatus and method for assaying sample fluid
WO2015125799A1 (en) * 2014-02-21 2015-08-27 有限会社ＩＴＤＮ－Ｔｏｋｙｏ Implant body
CN104609029A (en) * 2015-01-16 2015-05-13 江南大学 Antibacterial surface adopting surface micro-pattern design and antibacterial film
DE102017102609A1 (en) 2016-02-09 2017-08-10 Comprisetec Gmbh Connection of components by means of surface structures
EP3416591B1 (en) 2016-02-16 2021-10-27 Koninklijke Philips N.V. Surface topographies for altering the physiology of living cells
DE102017115704A1 (en) 2016-07-12 2018-04-12 Comprisetec Gmbh Component for reversible adhesive adhesion to a smooth surface, kit and manufacturing process
DE102018132120B4 (en) * 2018-12-13 2024-04-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Sample collection device for biological samples with a sample holder made of carbon-based material

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JPS60204643A (en) * 1984-03-30 1985-10-16 Sekisui Chem Co Ltd Interlayer for sandwich glass
GB2233334A (en) * 1989-06-29 1991-01-09 Exitech Ltd Surface treatment of polymer materials by the action of pulses of UV radiation
DE4141352A1 (en) * 1991-12-14 1993-06-17 Basf Ag METHOD FOR PRODUCING MICROSTRUCTURE BODIES
DE4225554A1 (en) * 1992-08-03 1994-02-10 Basf Magnetics Gmbh Flat polyethylene terephthalate materials with low surface roughness as well as a process for their production and their use
US5425977A (en) * 1993-08-16 1995-06-20 Monsanto Company Rough-surfaced interlayer
EP0731490A3 (en) * 1995-03-02 1998-03-11 Ebara Corporation Ultra-fine microfabrication method using an energy beam
EP0732624B1 (en) * 1995-03-17 2001-10-10 Ebara Corporation Fabrication method with energy beam
DE19818956A1 (en) * 1997-05-23 1998-11-26 Huels Chemische Werke Ag Materials e.g. polymer, metal or glass with micro-roughened, bacteria-repellent surface
DE19803787A1 (en) * 1998-01-30 1999-08-05 Creavis Tech & Innovation Gmbh Structured surfaces with hydrophobic properties
DE19914007A1 (en) * 1999-03-29 2000-10-05 Creavis Tech & Innovation Gmbh Structured liquid-repellent surfaces with locally defined liquid-wetting parts

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7374903B2 (en) 2001-06-13 2008-05-20 The University Of Liverpool Substrates with topographical features for the manipulation of cellular behavior and response
US7195872B2 (en) 2001-11-09 2007-03-27 3D Biosurfaces, Inc. High surface area substrates for microarrays and methods to make same
US20120141743A1 (en) * 2009-03-16 2012-06-07 Malgorzata-Jadwiga Kolodziej Biocide coating comprising copper
US9085019B2 (en) 2010-10-28 2015-07-21 3M Innovative Properties Company Superhydrophobic films
US9993948B2 (en) 2010-10-28 2018-06-12 3M Innovative Properties Company Superhydrophobic films
US9650661B2 (en) 2013-05-21 2017-05-16 3M Innovative Properties Company Nanostructured spore carrier
US10059977B2 (en) 2013-05-21 2018-08-28 3M Innovative Properties Company Biological sterilization indicator
EP2979844A4 (en) * 2014-04-22 2016-03-23 Sharp Kk Synthetic polymer membrane having surface with sterilizing activity, laminate equipped with synthetic polymer membrane, sterilization method utilizing surface of synthetic polymer membrane, method for reactivating surface of synthetic polymer membrane, mold for use in production of synthetic polymer membrane, and method for producing mold
US10968292B2 (en) 2017-09-26 2021-04-06 Sharp Kabushiki Kaisha Synthetic polymer film whose surface has microbicidal activity, photocurable resin composition, manufacturing method of synthetic polymer film, and sterilization method with use of surface of synthetic polymer film
US11364673B2 (en) 2018-02-21 2022-06-21 Sharp Kabushiki Kaisha Synthetic polymer film and production method of synthetic polymer film
US10934405B2 (en) 2018-03-15 2021-03-02 Sharp Kabushiki Kaisha Synthetic polymer film whose surface has microbicidal activity, plastic product which includes synthetic polymer film, sterilization method with use of surface of synthetic polymer film, photocurable resin composition, and manufacturing method of synthetic polymer film

Also Published As

Publication number Publication date
JP2001149061A (en) 2001-06-05
DE19950452A1 (en) 2001-04-26
EP1095760A1 (en) 2001-05-02

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