CA2322282A1 - Methode et compositions de detection differentielle de cellules tumorales et de cellules metastatiques - Google Patents

Methode et compositions de detection differentielle de cellules tumorales et de cellules metastatiques Download PDF

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Publication number
CA2322282A1
CA2322282A1 CA002322282A CA2322282A CA2322282A1 CA 2322282 A1 CA2322282 A1 CA 2322282A1 CA 002322282 A CA002322282 A CA 002322282A CA 2322282 A CA2322282 A CA 2322282A CA 2322282 A1 CA2322282 A1 CA 2322282A1
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CA
Canada
Prior art keywords
cells
sample
molecule
detector
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002322282A
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English (en)
Inventor
Emilio Barbera-Guillem
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
CLI Oncology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CLI Oncology Inc filed Critical CLI Oncology Inc
Publication of CA2322282A1 publication Critical patent/CA2322282A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57415—Specifically defined cancers of breast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/5743—Specifically defined cancers of skin, e.g. melanoma
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/54—Interleukins [IL]
    • G01N2333/55—IL-2

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une nouvelle méthode de détection et de dénombrement différentiels de cellules métastatiques présentes dans un échantillon de fluide biologique complexe, la méthode consistant à obtenir un échantillon de liquide organique provenant d'un individu; à ajouter à l'échantillon au moins deux molécules de détection, une première molécule de détection présentant une spécificité de liaison à une molécule cellulaire comprenant IL-2Ra, et une deuxième molécule de détection présentant une spécificité de liaison à une molécule cellulaire comprenant un antigène de cellule épithéliale et la fraction détectable de la première molécule de détection possédant un spectre d'émission maximal pouvant être distingué du spectre d'émission maximal de la fraction détectable de la deuxième molécule de détection; puis à analyser les cellules présentes dans l'échantillon dans un cytomètre de flux capable de détecter et de distinguer les spectres d'émission des première et deuxième molécules de détection et la diffusion de lumière de chaque cellule présente dans l'échantillon. L'invention concerne également une trousse de diagnostic clinique comprenant une molécule de détection présentant une spécificité de liaison avec IL-2Ra humain, et une molécule de détection présentant une spécificité de liaison avec un antigène de cellule épithéliale exprimé par des cellules humaines, la fraction détectable de la molécule de détection qui présente une spécificité de liaison avec IL-2Ra humain, possédant un spectre d'émission maximal pouvant être distingué du spectre d'émission maximal de la fraction détectable de la molécule de détection possédant une spécificité de liaison avec un antigène de cellule épithéliale exprimé par des cellules humaines. Le dessin constitue un histogramme représentant un antigène de cellule épithéliale (Ber-EP4 + cellules à fonctionnement commandé (FS)) et IL-2Ra + cellules (CD25).
CA002322282A 1998-02-27 1999-02-24 Methode et compositions de detection differentielle de cellules tumorales et de cellules metastatiques Abandoned CA2322282A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3204898A 1998-02-27 1998-02-27
US09/032,048 1998-02-27
PCT/US1999/004005 WO1999044064A1 (fr) 1998-02-27 1999-02-24 Methode et compositions de detection differentielle de cellules tumorales et de cellules metastatiques

Publications (1)

Publication Number Publication Date
CA2322282A1 true CA2322282A1 (fr) 1999-09-02

Family

ID=21862819

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002322282A Abandoned CA2322282A1 (fr) 1998-02-27 1999-02-24 Methode et compositions de detection differentielle de cellules tumorales et de cellules metastatiques

Country Status (3)

Country Link
AU (1) AU2785699A (fr)
CA (1) CA2322282A1 (fr)
WO (1) WO1999044064A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
DE50209932D1 (de) * 2001-09-06 2007-05-24 Adnagen Gmbh Verfahren und kit zur diagnostik oder behandlungskontrolle von darmkrebs
ATE309393T1 (de) 2001-09-06 2005-11-15 Adnagen Ag Verfahren zum qualitativen und/oder quantitativen nachweis von zellen
WO2003023060A2 (fr) * 2001-09-06 2003-03-20 Adnagen Ag Methode et trousse de diagnostic ou de surveillance du traitement du cancer du sein
AU2003216175A1 (en) 2002-02-04 2003-09-02 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
EP2359689B1 (fr) 2002-09-27 2015-08-26 The General Hospital Corporation Dispositif microfluidique pour la séparation de cellules et usage du dispositif
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9487812B2 (en) 2012-02-17 2016-11-08 Colorado School Of Mines Optical alignment deformation spectroscopy
US9885644B2 (en) 2006-01-10 2018-02-06 Colorado School Of Mines Dynamic viscoelasticity as a rapid single-cell biomarker
US9878326B2 (en) 2007-09-26 2018-01-30 Colorado School Of Mines Fiber-focused diode-bar optical trapping for microfluidic manipulation
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US10722250B2 (en) 2007-09-04 2020-07-28 Colorado School Of Mines Magnetic-field driven colloidal microbots, methods for forming and using the same
EP2334812B1 (fr) 2008-09-20 2016-12-21 The Board of Trustees of The Leland Stanford Junior University Diagnostic non invasif d'aneuploïdie foetale par sequençage

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
WO1994020120A1 (fr) * 1993-03-12 1994-09-15 Cellcor, Inc. Methode de titrage in vitro pour la mesure du degre d'activation de cellules immunes
US5536642A (en) * 1993-09-09 1996-07-16 Barbera-Guillem; Emilio Diagnostic and prognostic methods for solid non-lymphoid tumors and their metastases

Also Published As

Publication number Publication date
WO1999044064A1 (fr) 1999-09-02
AU2785699A (en) 1999-09-15

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Legal Events

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FZDE Discontinued