CA2316755C - Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs - Google Patents

Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs Download PDF

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Publication number
CA2316755C
CA2316755C CA002316755A CA2316755A CA2316755C CA 2316755 C CA2316755 C CA 2316755C CA 002316755 A CA002316755 A CA 002316755A CA 2316755 A CA2316755 A CA 2316755A CA 2316755 C CA2316755 C CA 2316755C
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CA
Canada
Prior art keywords
cells
antibodies
bone marrow
marine
displayed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002316755A
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English (en)
Other versions
CA2316755A1 (fr
Inventor
Katherine S. Bowdish
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alexion Antibody Technologies Inc
Original Assignee
Alexion Antibody Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Antibody Technologies Inc filed Critical Alexion Antibody Technologies Inc
Priority to CA002431846A priority Critical patent/CA2431846A1/fr
Publication of CA2316755A1 publication Critical patent/CA2316755A1/fr
Application granted granted Critical
Publication of CA2316755C publication Critical patent/CA2316755C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention se rapporte à un procédé d'identification d'anticorps agonistes ou inhibiteurs de récepteurs impliqués dans la prolifération et la différentiation cellulaires et la survie et l'activation de cellules. Ledit procédé consiste à immuniser un animal avec des cellules souches ayant des molécules de surface comportant les récepteurs, de façon à générer une pluralité de cellules exprimant un ou plusieurs anticorps dirigés contre lesdites molécules de surface, à créer une banque de composés à partir de cet ensemble de cellules et de séquences d'acides nucléiques codant les anticorps, à cloner les séquences d'acides nucléiques provenant de la librairie dans des vecteurs d'affichage superficiel de sorte que les anticorps sont affichés en surface, et à trier ces anticorps affichés en surface au moyen de cellules cibles dans le but d'identifier des anticorps agonistes ou inhibiteurs des récepteurs.
CA002316755A 1998-01-23 1999-01-22 Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs Expired - Fee Related CA2316755C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002431846A CA2431846A1 (fr) 1998-01-23 1999-01-22 Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7225398P 1998-01-23 1998-01-23
US60/072,253 1998-01-23
PCT/US1999/001331 WO1999038008A1 (fr) 1998-01-23 1999-01-22 Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002431846A Division CA2431846A1 (fr) 1998-01-23 1999-01-22 Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs

Publications (2)

Publication Number Publication Date
CA2316755A1 CA2316755A1 (fr) 1999-07-29
CA2316755C true CA2316755C (fr) 2006-04-04

Family

ID=22106479

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002316755A Expired - Fee Related CA2316755C (fr) 1998-01-23 1999-01-22 Procedes et compositions d'identification de substances mimetiques de facteurs de croissance, de facteurs de croissance et d'inhibiteurs

Country Status (5)

Country Link
EP (1) EP1047942A4 (fr)
JP (2) JP3515072B2 (fr)
AU (1) AU2333999A (fr)
CA (1) CA2316755C (fr)
WO (1) WO1999038008A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0001401D0 (en) * 2000-01-22 2000-03-08 Univ Edinburgh Methods for amplifying genetic material and use thereof
IL154159A0 (en) 2000-08-01 2003-07-31 Yissum Res Dev Co Directed differentiation of ebryonic cells
WO2002033073A1 (fr) * 2000-10-20 2002-04-25 Chugai Seiyaku Kabushiki Kaisha Anticorps agoniste degrade
WO2003091424A1 (fr) 2002-04-26 2003-11-06 Chugai Seiyaku Kabushiki Kaisha Procede de criblage d'un anticorps agoniste
JP2004016021A (ja) 2002-06-12 2004-01-22 Japan Science & Technology Corp 抗体および阻害剤並びにそれを用いた形質転換方法および形質転換キット
US7833979B2 (en) 2005-04-22 2010-11-16 Amgen Inc. Toxin peptide therapeutic agents
US8008453B2 (en) 2005-08-12 2011-08-30 Amgen Inc. Modified Fc molecules
US7825093B2 (en) 2006-10-25 2010-11-02 Amgen Inc. Methods of using OSK1 peptide analogs
CA2840407A1 (fr) 2007-05-22 2008-12-18 Amgen Inc. Compositions et procedes pour produire des proteines de fusion bioactives

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993014188A1 (fr) * 1992-01-17 1993-07-22 The Regents Of The University Of Michigan Virus cible
WO1993018136A1 (fr) * 1992-03-05 1993-09-16 Cytomed, Inc. Procede de maintien de cellules souche hematopoietiques
IL106255A0 (en) * 1993-04-23 1993-11-15 Baxter Int Method for isolating human blood cells
US5554512A (en) * 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
US5635388A (en) * 1994-04-04 1997-06-03 Genentech, Inc. Agonist antibodies against the flk2/flt3 receptor and uses thereof
US5753516A (en) * 1995-02-03 1998-05-19 Heagy; Wyrta E. Screening method for ligands of the EBI-1 receptor
US5686292A (en) * 1995-06-02 1997-11-11 Genentech, Inc. Hepatocyte growth factor receptor antagonist antibodies and uses thereof

Also Published As

Publication number Publication date
AU2333999A (en) 1999-08-09
JP2004123695A (ja) 2004-04-22
EP1047942A4 (fr) 2002-10-30
WO1999038008A1 (fr) 1999-07-29
JP2003517566A (ja) 2003-05-27
EP1047942A1 (fr) 2000-11-02
CA2316755A1 (fr) 1999-07-29
JP3515072B2 (ja) 2004-04-05

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EEER Examination request
MKLA Lapsed
MKLA Lapsed

Effective date: 20080122