CA2301077C - Multi-layer testing column - Google Patents
Multi-layer testing column Download PDFInfo
- Publication number
- CA2301077C CA2301077C CA2301077A CA2301077A CA2301077C CA 2301077 C CA2301077 C CA 2301077C CA 2301077 A CA2301077 A CA 2301077A CA 2301077 A CA2301077 A CA 2301077A CA 2301077 C CA2301077 C CA 2301077C
- Authority
- CA
- Canada
- Prior art keywords
- layer
- chamber
- column according
- membrane layers
- layer column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 75
- 239000012528 membrane Substances 0.000 claims abstract description 83
- 239000012491 analyte Substances 0.000 claims abstract description 62
- 239000000758 substrate Substances 0.000 claims abstract description 22
- 239000007790 solid phase Substances 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims description 61
- 239000003153 chemical reaction reagent Substances 0.000 claims description 33
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 239000011534 wash buffer Substances 0.000 claims description 16
- 239000000427 antigen Substances 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 239000003446 ligand Substances 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 9
- 230000005284 excitation Effects 0.000 claims description 8
- 239000012634 fragment Substances 0.000 claims description 8
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 6
- 108020003175 receptors Proteins 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 230000004071 biological effect Effects 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 4
- 239000012982 microporous membrane Substances 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000003547 immunosorbent Substances 0.000 claims description 2
- 230000031700 light absorption Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 59
- 230000008901 benefit Effects 0.000 description 10
- 230000027455 binding Effects 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 230000001276 controlling effect Effects 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- -1 polyethersulphone Polymers 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 230000009871 nonspecific binding Effects 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000006226 wash reagent Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 239000003570 air Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000007876 drug discovery Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 108091023037 Aptamer Proteins 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229920006243 acrylic copolymer Polymers 0.000 description 1
- 108010004469 allophycocyanin Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- GWQVMPWSEVRGPY-UHFFFAOYSA-N europium cryptate Chemical compound [Eu+3].N=1C2=CC=CC=1CN(CC=1N=C(C=CC=1)C=1N=C(C3)C=CC=1)CC(N=1)=CC(C(=O)NCCN)=CC=1C(N=1)=CC(C(=O)NCCN)=CC=1CN3CC1=CC=CC2=N1 GWQVMPWSEVRGPY-UHFFFAOYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N2030/524—Physical parameters structural properties
- G01N2030/527—Physical parameters structural properties sorbent material in form of a membrane
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1055—General features of the devices using the transfer device for another function for immobilising reagents, e.g. dried reagents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6082—Construction of the column body transparent to radiation
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Materials For Medical Uses (AREA)
- Memory System Of A Hierarchy Structure (AREA)
- Prostheses (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9046998P | 1998-06-24 | 1998-06-24 | |
| US60/090,469 | 1998-06-24 | ||
| PCT/US1999/014118 WO1999067647A1 (en) | 1998-06-24 | 1999-06-23 | Multi-layer testing column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2301077A1 CA2301077A1 (en) | 1999-12-29 |
| CA2301077C true CA2301077C (en) | 2010-05-25 |
Family
ID=22222904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2301077A Expired - Lifetime CA2301077C (en) | 1998-06-24 | 1999-06-23 | Multi-layer testing column |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20040105782A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1004025B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2002519643A (cg-RX-API-DMAC7.html) |
| CN (1) | CN1158530C (cg-RX-API-DMAC7.html) |
| AT (1) | ATE257944T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU4827999A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2301077C (cg-RX-API-DMAC7.html) |
| DE (1) | DE69914164T2 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2214865T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO1999067647A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7799521B2 (en) * | 1998-06-24 | 2010-09-21 | Chen & Chen, Llc | Thermal cycling |
| US6780617B2 (en) | 2000-12-29 | 2004-08-24 | Chen & Chen, Llc | Sample processing device and method |
| US20030032029A1 (en) * | 1998-12-21 | 2003-02-13 | Nanogen, Inc. | Three dimensional apparatus and method for integrating sample preparation and multiplex assays |
| AU771499B2 (en) * | 1999-07-26 | 2004-03-25 | Government of The United States of America, as represented by The Secretary Department of Health & Human Services, The National Institutes of Health, The | Layered device with capture regions for cellular analysis |
| US7214477B1 (en) | 1999-07-26 | 2007-05-08 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Layered device with capture regions for cellular analysis |
| AU2002249206A1 (en) * | 2001-02-13 | 2002-08-28 | Universiteit Gent | Device and method for detecting the presence of an analyte |
| AU2002341644B2 (en) | 2001-09-11 | 2008-02-28 | Iquum, Inc. | Sample vessels |
| WO2004048928A2 (en) | 2002-11-25 | 2004-06-10 | The United States Of America As Represented By The Secretary Of Health And Human Services, Nih | Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array |
| AU2004220626B2 (en) | 2003-02-05 | 2010-07-29 | Iquum Inc. | Sample processing tubule |
| US20070017870A1 (en) | 2003-09-30 | 2007-01-25 | Belov Yuri P | Multicapillary device for sample preparation |
| CN101432698B (zh) * | 2004-06-07 | 2012-06-06 | 伊库姆公司 | 处理样品的方法 |
| DE102005005437A1 (de) | 2005-02-05 | 2006-08-10 | Eppendorf Ag | Filterpipettenspitze |
| US20080003564A1 (en) * | 2006-02-14 | 2008-01-03 | Iquum, Inc. | Sample processing |
| GB2435512A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | A binding assay and assay device |
| GB2435511A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Protease detection |
| GB2435510A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
| CA3061952C (en) * | 2006-03-09 | 2022-07-19 | Aethlon Medical, Inc. | Extracorporeal removal of microvesicular particles |
| ES2690319T3 (es) | 2006-06-06 | 2018-11-20 | F. Hoffmann-La Roche Ag | Hemólisis diferencial de una muestra de sangre completa |
| US20080102493A1 (en) * | 2006-06-29 | 2008-05-01 | Millipore Corporation | Isolation of RNA and DNA from a biological sample |
| JP4935424B2 (ja) * | 2007-02-28 | 2012-05-23 | 東レ株式会社 | 免疫分析チップ |
| AU2009228086B2 (en) * | 2008-03-28 | 2015-04-23 | Biotix Inc. | Sample preparation devices and methods for processing analytes |
| CN102841167A (zh) * | 2011-06-20 | 2012-12-26 | 苏州赛分科技有限公司 | 一种液相吸附色谱分离柱 |
| WO2015048769A1 (en) * | 2013-09-30 | 2015-04-02 | Board Of Regents, The University Of Texas System | Method and apparatus for scanning detection |
| US11260386B2 (en) | 2015-06-05 | 2022-03-01 | The Emerther Company | Component of a device, a device, and a method for purifying and testing biomolecules from biological samples |
| EP3488237B1 (en) * | 2016-07-25 | 2021-09-01 | Bio-Rad Laboratories, Inc. | Lateral flow device and method of use |
| WO2020214224A1 (en) * | 2019-04-18 | 2020-10-22 | Siemens Healthcare Diagnostics Inc. | Integrated microfluidic device with pipette adaptation |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2942674B1 (de) * | 1979-10-23 | 1981-05-07 | Drägerwerk AG, 2400 Lübeck | Pruefroehrchen zur Messung von Nickel-Aerosolen |
| EP0139373A1 (en) * | 1983-08-26 | 1985-05-02 | The Regents Of The University Of California | Multiple immunoassay system |
| US5057438A (en) * | 1986-09-24 | 1991-10-15 | Agency Of Industrial Science And Technology | Electrophoretic antigen-antibody determination with laminate of multiple membranes |
| US4874691A (en) * | 1987-10-16 | 1989-10-17 | Quadra Logic Technologies Inc. | Membrane-supported immunoassays |
| US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| WO1994020831A1 (en) * | 1993-03-08 | 1994-09-15 | Norman Wainwright | Aligned fiber diagnostic chromatography |
-
1999
- 1999-06-23 CN CNB998010111A patent/CN1158530C/zh not_active Expired - Lifetime
- 1999-06-23 ES ES99931860T patent/ES2214865T3/es not_active Expired - Lifetime
- 1999-06-23 AT AT99931860T patent/ATE257944T1/de not_active IP Right Cessation
- 1999-06-23 JP JP2000556253A patent/JP2002519643A/ja active Pending
- 1999-06-23 CA CA2301077A patent/CA2301077C/en not_active Expired - Lifetime
- 1999-06-23 DE DE69914164T patent/DE69914164T2/de not_active Expired - Lifetime
- 1999-06-23 AU AU48279/99A patent/AU4827999A/en not_active Abandoned
- 1999-06-23 WO PCT/US1999/014118 patent/WO1999067647A1/en not_active Ceased
- 1999-06-23 EP EP99931860A patent/EP1004025B1/en not_active Expired - Lifetime
-
2003
- 2003-09-30 US US10/605,459 patent/US20040105782A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1004025B1 (en) | 2004-01-14 |
| CA2301077A1 (en) | 1999-12-29 |
| CN1273638A (zh) | 2000-11-15 |
| WO1999067647A1 (en) | 1999-12-29 |
| ES2214865T3 (es) | 2004-09-16 |
| ATE257944T1 (de) | 2004-01-15 |
| EP1004025A1 (en) | 2000-05-31 |
| US20040105782A1 (en) | 2004-06-03 |
| DE69914164T2 (de) | 2004-11-25 |
| CN1158530C (zh) | 2004-07-21 |
| AU4827999A (en) | 2000-01-10 |
| JP2002519643A (ja) | 2002-07-02 |
| DE69914164D1 (de) | 2004-02-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20190625 |