CA2290738A1 - Ensembles d'acide nucleique - Google Patents

Ensembles d'acide nucleique Download PDF

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Publication number
CA2290738A1
CA2290738A1 CA002290738A CA2290738A CA2290738A1 CA 2290738 A1 CA2290738 A1 CA 2290738A1 CA 002290738 A CA002290738 A CA 002290738A CA 2290738 A CA2290738 A CA 2290738A CA 2290738 A1 CA2290738 A1 CA 2290738A1
Authority
CA
Canada
Prior art keywords
array
polynucleotide
polynucleotides
gene
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002290738A
Other languages
English (en)
Inventor
Alex Chenchik
George Jokhadze
Robert Bibilashvilli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio USA Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/859,998 external-priority patent/US5994076A/en
Application filed by Individual filed Critical Individual
Publication of CA2290738A1 publication Critical patent/CA2290738A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

On décrit des ensembles de taches de polynucléotides et des kits comprenant ces ensembles, ainsi que des procédés de préparation et d'utilisation de ces derniers. Les ensembles de cette invention comprennent plusieurs taches de polynucléotides qui sont associées de manière stable à la surface d'un support solide. Au moins une partie des taches de polynucléotides comprend une composition de sonde polynucléotidique qui est constituée de polynucléotides uniques, dans laquelle tous les polynucléotides uniques de l'ensemble correspondent à un type commun de gène. On décrit également des groupes d'un nombre représentatif d'amorces spécifiques à un gène appropriées pour être utilisées dans la production d'acide nucléique cible destiné à être utilisé avec les ensembles de cette invention. Ces ensembles sont utiles dans des dosages d'hybridation, plus particulièrement dans des dosages permettant d'identifier des modèles d'expression de gènes différentiels parmi deux ou plusieurs types différents de cellules.
CA002290738A 1997-05-21 1998-05-21 Ensembles d'acide nucleique Abandoned CA2290738A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/859,998 US5994076A (en) 1997-05-21 1997-05-21 Methods of assaying differential expression
US08/859,998 1997-05-21
US5337598A 1998-03-31 1998-03-31
US09/053,375 1998-03-31
PCT/US1998/010561 WO1998053103A1 (fr) 1997-05-21 1998-05-21 Ensembles d'acide nucleique

Publications (1)

Publication Number Publication Date
CA2290738A1 true CA2290738A1 (fr) 1998-11-26

Family

ID=26731788

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002290738A Abandoned CA2290738A1 (fr) 1997-05-21 1998-05-21 Ensembles d'acide nucleique

Country Status (5)

Country Link
EP (1) EP0988398A4 (fr)
JP (1) JP2002504812A (fr)
AU (1) AU742342B2 (fr)
CA (1) CA2290738A1 (fr)
WO (1) WO1998053103A1 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306643B1 (en) 1998-08-24 2001-10-23 Affymetrix, Inc. Methods of using an array of pooled probes in genetic analysis
EP1115737A4 (fr) * 1998-09-23 2004-11-24 Cleveland Clinic Foundation Nouveaux genes stimules ou reprimes par l'interferon
AU2395900A (en) * 1998-12-31 2000-07-31 Gene Logic, Inc. Assay device comprising mixed probes
WO2000052209A1 (fr) * 1999-03-02 2000-09-08 Chiron Corporation Microarrays permettant d'identifier l'activation ou l'induction de voies biochimiques
WO2000065095A2 (fr) * 1999-04-23 2000-11-02 Clontech Laboratories, Inc. Methodes et compositions de realisation de dosages d'hybridation reposant sur des jeux ordonnes d'echantillons
WO2000066781A2 (fr) * 1999-04-30 2000-11-09 Ball Semiconductor, Inc. Billes d'adn
US6268141B1 (en) * 1999-05-12 2001-07-31 Beckman Coulter, Inc. Immobilization of unmodified biopolymers to acyl fluoride activated substrates
DE19922052A1 (de) * 1999-05-14 2000-11-16 Theragen Molekularmedizinische Verfahren zum Nachweis der Wirkung von unterschiedlichen Chemotherapeutika und/oder einer Strahlentherapie bei malignen Erkrankungen sowie Verfahren zur Auswahl wirkungsvoller therapeutischer Mittel zu deren Therapie
AU776129B2 (en) 1999-05-21 2004-08-26 Invitrogen Corporation Compositions and methods for labeling of nucleic acid molecules
EP1185701A1 (fr) * 1999-06-11 2002-03-13 Clingenix, Inc. Reseaux specifiques de genes et leur utilisation
US6716579B1 (en) 1999-06-11 2004-04-06 Narayan Baidya Gene specific arrays, preparation and use
US20010053849A1 (en) * 1999-06-16 2001-12-20 Mary Jeanne Kreek Plural biological sample arrays, and preparation and uses thereof
AU6382000A (en) * 1999-07-27 2001-02-13 Iowa State University Research Foundation Inc. Genome analysis
EP1498146A3 (fr) 1999-10-06 2007-01-24 Tigenix N.V. Analyse in vivo et marqueurs moléculaires pour tester la stabilité phénotypique.
US6383749B2 (en) 1999-12-02 2002-05-07 Clontech Laboratories, Inc. Methods of labeling nucleic acids for use in array based hybridization assays
EP1250460A2 (fr) 2000-01-14 2002-10-23 Integriderm L.L.C. Jeux ordonnes d'echantillons d'acides nucleiques informatifs et methodes de production associees
AU2001251086A1 (en) * 2000-03-31 2001-10-15 Sir Mortimer B. Davis Jewish General Hospital Microchip arrays of regulatory genes
WO2001083827A1 (fr) * 2000-05-04 2001-11-08 Yale University Reseaux de proteines a haute densite destines au criblage de l'activite de proteines
AU2001292959A1 (en) 2000-09-22 2002-04-02 Clontech Laboratories, Inc. Highly sensitive proteomic analysis methods and kits and systems for practicing the same
US6897067B2 (en) 2000-11-03 2005-05-24 Regents Of The University Of Michigan Surface transfection and expression procedure
US6902933B2 (en) 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
JP2005505231A (ja) 2000-11-03 2005-02-24 リージェンツ オブ ザ ユニバーシティ オブ ミシガン 表面トランスフェクションおよび発現法
DE10065666B4 (de) * 2000-12-29 2008-10-02 Wiesner, Rudolf, Prof. Dr. DNA-Chip zur kausalen Diagnose von Bluthochdruck
DE10100122A1 (de) * 2001-01-03 2002-07-11 Henkel Kgaa Verfahren zur Bestimmung der Hautalterung in vitro
JP3694674B2 (ja) 2001-02-28 2005-09-14 株式会社日立製作所 オリゴヌクレオチドアレイおよび検査方法
EP1270740A1 (fr) * 2001-06-29 2003-01-02 SIRS-Lab GmbH Biopuce et son utilisation pour la détermination de l'inflammation
BE1014949A3 (fr) * 2001-08-14 2004-07-06 Probiox Procede de detection de stress oxydant et trousse pour sa mise en oeuvre.
WO2003016327A1 (fr) * 2001-08-14 2003-02-27 Mount Sinai School Of Medicine Utilisation de reporters intrinseques de signaux de cellules pour le profilage de medicaments a fort contenu et le criblage de toxicites
AU2002337442A1 (en) * 2001-09-24 2003-04-07 University Of Aarhus Novel compositions and methods for diagnosis and treatment of lymphoma and leukemia
DE10155600B4 (de) * 2001-11-09 2009-08-27 Oligene Gmbh Nukleinsäure-Array
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CA2544041C (fr) * 2003-10-28 2015-12-08 Bioarray Solutions Ltd. Optimisation de l'analyse de l'expression genique a l'aide de sondes de capture immobilisees
US8592368B2 (en) 2003-12-19 2013-11-26 University Of South Florida JAK/STAT inhibitors and MAPK/ERK inhibitors for RSV infection
DE102004037081A1 (de) * 2004-07-30 2006-03-23 Universität Bremen Verfahren zum Analysieren von Proben mittels einer Hybridisierung
US20060147963A1 (en) * 2004-12-30 2006-07-06 Affymetrix, Inc. Detection of polynucleotides on nucleic acid arrays using azido-modified triphosphate nucleotide analogs
EP2093566A1 (fr) * 2008-02-21 2009-08-26 Pangaea Biotech, S.A. Procédé pour le pronostic du cancer pulmonaire à grandes cellules
EP2093567A1 (fr) * 2008-02-21 2009-08-26 Pangaea Biotech, S.A. Niveaux d'expression d'ARNm Brca1 pour prédire la survie chez des patients atteints de cancer du sein traités par chimiothérapie néoadjuvante

Also Published As

Publication number Publication date
AU7593398A (en) 1998-12-11
EP0988398A1 (fr) 2000-03-29
EP0988398A4 (fr) 2005-05-18
AU742342B2 (en) 2001-12-20
JP2002504812A (ja) 2002-02-12
WO1998053103A1 (fr) 1998-11-26

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Legal Events

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EEER Examination request
FZDE Discontinued