CA2257867A1 - Editing-based selectable plastid marker genes - Google Patents
Editing-based selectable plastid marker genesInfo
- Publication number
- CA2257867A1 CA2257867A1 CA002257867A CA2257867A CA2257867A1 CA 2257867 A1 CA2257867 A1 CA 2257867A1 CA 002257867 A CA002257867 A CA 002257867A CA 2257867 A CA2257867 A CA 2257867A CA 2257867 A1 CA2257867 A1 CA 2257867A1
- Authority
- CA
- Canada
- Prior art keywords
- marker genes
- editing
- based selectable
- disclosed
- plastid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003550 marker Substances 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 210000002706 plastid Anatomy 0.000 title abstract 3
- 241000196324 Embryophyta Species 0.000 abstract 2
- 238000002955 isolation Methods 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Disclosed are novel DNA constructs for selecting plastid transformants in higher plants. Also disclosed are editing based selectable marker genes which require editing at the transcriptional level for expression of the selectable marker gene. Vectors including such edited upstream sequences operably linked to slectable marker genes facilitate the isolation of plastid, rather than nuclear transformants in higher plants.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1974196P | 1996-06-14 | 1996-06-14 | |
US60/019,741 | 1996-06-14 | ||
PCT/US1997/010318 WO1997047771A1 (en) | 1996-06-14 | 1997-06-13 | Editing-based selectable plastid marker genes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2257867A1 true CA2257867A1 (en) | 1997-12-18 |
CA2257867C CA2257867C (en) | 2006-08-01 |
Family
ID=36764364
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002257867A Expired - Lifetime CA2257867C (en) | 1996-06-14 | 1997-06-13 | Editing-based selectable plastid marker genes |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2257867C (en) |
-
1997
- 1997-06-13 CA CA002257867A patent/CA2257867C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2257867C (en) | 2006-08-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20170613 |