WO1999005265A3 - Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance - Google Patents

Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance Download PDF

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Publication number
WO1999005265A3
WO1999005265A3 PCT/US1998/015289 US9815289W WO9905265A3 WO 1999005265 A3 WO1999005265 A3 WO 1999005265A3 US 9815289 W US9815289 W US 9815289W WO 9905265 A3 WO9905265 A3 WO 9905265A3
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WO
WIPO (PCT)
Prior art keywords
herbicide resistance
plants
production
plastid transformation
transgenic plants
Prior art date
Application number
PCT/US1998/015289
Other languages
French (fr)
Other versions
WO1999005265A2 (en
Inventor
Alan D Blowers
John Sanford
Ana Maria Bailey
Original Assignee
Sanford Scient Inc
Invest Y De Estudios Avanzados
Alan D Blowers
John Sanford
Ana Maria Bailey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanford Scient Inc, Invest Y De Estudios Avanzados, Alan D Blowers, John Sanford, Ana Maria Bailey filed Critical Sanford Scient Inc
Priority to AU85837/98A priority Critical patent/AU8583798A/en
Publication of WO1999005265A2 publication Critical patent/WO1999005265A2/en
Publication of WO1999005265A3 publication Critical patent/WO1999005265A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method of producing a herbicide-resistant plant, which method entails delivering one or more herbicide resistance-conferring selectable marker genes into and expressing the same within the plastid of the plant, both in photosynthetic as well as non-photosynthetic cells. Nucleic acids for transformation and multicellular plants whose plastids have been transformed are also provided.
PCT/US1998/015289 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance WO1999005265A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85837/98A AU8583798A (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of trans genic plants with herbicide resistance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US89906197A 1997-07-23 1997-07-23
US08/899,061 1997-07-23

Publications (2)

Publication Number Publication Date
WO1999005265A2 WO1999005265A2 (en) 1999-02-04
WO1999005265A3 true WO1999005265A3 (en) 1999-05-20

Family

ID=25410433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015289 WO1999005265A2 (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance

Country Status (4)

Country Link
AR (1) AR015136A1 (en)
AU (1) AU8583798A (en)
WO (1) WO1999005265A2 (en)
ZA (1) ZA986574B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
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US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form

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US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
CA2332700A1 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids
US6492578B1 (en) 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
CN1219064C (en) 1998-08-04 2005-09-14 嘉吉有限公司 Plant fatty acid desaturase promoters
US6515206B1 (en) * 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
WO2001007590A2 (en) * 1999-07-27 2001-02-01 Syngenta Participations Ag Chimeric genes for plastid expression
CN101311267B (en) 1999-12-16 2016-08-03 孟山都技术有限公司 Novel plant expression constructs
ATE553117T1 (en) * 2000-02-29 2012-04-15 Univ Auburn MULTIPLE GENE EXPRESSION TO ESTABLISH NEW SYNTHETIC PATHWAYS AND OVEREXPRESSION OF FOREIGN PROTEINS IN PLANTS
US20030041353A1 (en) * 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
CN100480376C (en) 2000-03-22 2009-04-22 依康遗传学股份有限公司 Method for transforming plant plastides and making transplastomic plants
JP2003530888A (en) * 2000-04-20 2003-10-21 ビーティージー・インターナショナル・リミテッド Transgenic plants
US6781033B2 (en) 2000-04-26 2004-08-24 Monsanto Technology Llc Method for the transformation of plant cell plastids
BR0113156A (en) * 2000-08-11 2003-07-08 Syngenta Participations Ag Method for producing a transformed dicotyledonous plant, transformed plant cell, multiple shoot culture, transformed plant, seed produced by the transformed plant, method for producing a plant comprising a transformed plastid genome, transformed plastid genome, and, plastid
WO2004016793A2 (en) * 2002-08-08 2004-02-26 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in asteraceae, vector for use therein and plants thus obtained
AU2012200277B2 (en) * 2002-08-08 2014-11-06 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in Asteraceae, vector for use therein and plants thus obtained
US8536429B2 (en) 2006-07-12 2013-09-17 Commonwealth Scientific And Industrial Research Organisation Polynucleotides encoding a NAX2 polypeptide and methods for enhancing salinity tolerance in plants
CA2671365C (en) * 2006-12-07 2020-07-21 Dow Agrosciences Llc Use of dsm-2 gene as a selectable marker for selecting herbicidal resistant plants
WO2009003222A1 (en) 2007-06-29 2009-01-08 Commonwealth Scientific And Industrial Research Organisation Methods for degrading toxic compounds
US8642846B2 (en) 2007-08-13 2014-02-04 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordeins
WO2009129583A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Recombinant cells and methods for hydroxylating fatty acids
ES2486665T3 (en) 2008-08-25 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Resistance genes
CN102317459A (en) 2008-11-18 2012-01-11 联邦科学技术研究组织 Enzymes and methods for producing omega-3 fatty acids
AR083323A1 (en) 2010-06-28 2013-02-21 Commw Scient Ind Res Org METHODS TO PRODUCE LIPIDS
WO2013096991A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
CA2860432C (en) 2011-12-27 2024-06-04 Commonwealth Scientific And Industrial Research Organisation Simultaneous gene silencing and suppressing gene silencing in the same cell
MX2014007964A (en) 2011-12-27 2014-10-06 Commw Scient Ind Res Org Processes for producing lipids.
AU2014280852B2 (en) 2013-06-13 2017-11-09 Commonwealth Scientific And Industrial Research Organisation Barley with very low levels of hordeins
AU2014308558B2 (en) 2013-08-21 2020-11-12 Commonwealth Scientific And Industrial Research Organisation Rust resistance gene
US9725399B2 (en) 2013-12-18 2017-08-08 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
KR102527795B1 (en) 2014-06-27 2023-05-02 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 Lipid comprising docosapentaenoic acid
EP2966157B1 (en) 2014-07-07 2023-11-01 Nuseed Global Innovation Ltd Processes for producing industrial products from plant lipids
AU2016355920B2 (en) 2015-11-18 2020-11-12 Commonwealth Scientific And Industrial Research Organisation Rice grain with thickened aleurone
US10941428B2 (en) 2018-09-12 2021-03-09 Universidad Politécnica de Madrid Reagents and methods for the expression of an active NifB protein and uses thereof

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EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
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US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
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US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
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Patent Citations (7)

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EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
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WO1998002562A2 (en) * 1996-07-16 1998-01-22 Rhone-Poulenc Agrochimie Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form
US9550718B2 (en) 2012-06-15 2017-01-24 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids

Also Published As

Publication number Publication date
AU8583798A (en) 1999-02-16
WO1999005265A2 (en) 1999-02-04
AR015136A1 (en) 2001-04-18
ZA986574B (en) 2000-01-12

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