CA2245004A1 - Ice seeding apparatus for cryopreservation systems - Google Patents
Ice seeding apparatus for cryopreservation systemsInfo
- Publication number
- CA2245004A1 CA2245004A1 CA 2245004 CA2245004A CA2245004A1 CA 2245004 A1 CA2245004 A1 CA 2245004A1 CA 2245004 CA2245004 CA 2245004 CA 2245004 A CA2245004 A CA 2245004A CA 2245004 A1 CA2245004 A1 CA 2245004A1
- Authority
- CA
- Canada
- Prior art keywords
- ice
- container
- ice seeding
- cryopreservative
- liquefied
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000010899 nucleation Methods 0.000 title abstract 4
- 238000005138 cryopreservation Methods 0.000 title abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 230000002338 cryopreservative effect Effects 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 238000007599 discharging Methods 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissue equivalents wherein ice seeding is performed by discharging a liquefied or chilled gas (60) from a nozzle (21) to the surface of a container (40) containing said biological sample in cryopreservative. Inside a freezing chamber (70), containers (40) are placed on racks (30) that are located proximal to a sprayrail (20) containing nozzles (21). Ice seeding is performed by discharge of liquefied or chilled gas (60) from the nozzles to the container surface at the solid-liquid equilibrium temperature of the cryopreservative.
Localized cooling of the container surface by the discharge results in ice seed formation within the container (40).
Localized cooling of the container surface by the discharge results in ice seed formation within the container (40).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/593,587 | 1996-01-30 | ||
| US08/593,587 US5689961A (en) | 1996-01-30 | 1996-01-30 | Ice seeding apparatus for cryopreservation systems |
| PCT/US1997/001619 WO1997028402A1 (en) | 1996-01-30 | 1997-01-30 | Ice seeding apparatus for cryopreservation systems |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2245004A1 true CA2245004A1 (en) | 1997-08-07 |
| CA2245004C CA2245004C (en) | 2008-01-15 |
Family
ID=29422877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2245004 Expired - Lifetime CA2245004C (en) | 1996-01-30 | 1997-01-30 | Ice seeding apparatus for cryopreservation systems |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2245004C (en) |
-
1997
- 1997-01-30 CA CA 2245004 patent/CA2245004C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2245004C (en) | 2008-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20170130 |