CA2243850A1 - Promoteurs issus des framboises et permettant l'expression de transgenes dans des vegetaux - Google Patents

Promoteurs issus des framboises et permettant l'expression de transgenes dans des vegetaux Download PDF

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Publication number
CA2243850A1
CA2243850A1 CA 2243850 CA2243850A CA2243850A1 CA 2243850 A1 CA2243850 A1 CA 2243850A1 CA 2243850 CA2243850 CA 2243850 CA 2243850 A CA2243850 A CA 2243850A CA 2243850 A1 CA2243850 A1 CA 2243850A1
Authority
CA
Canada
Prior art keywords
gene
plant
promoter
seq
drul
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2243850
Other languages
English (en)
Inventor
Jill Anne Kellogg
Richard Keith Bestwick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exelixis Plant Sciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/592,936 external-priority patent/US5783393A/en
Application filed by Individual filed Critical Individual
Publication of CA2243850A1 publication Critical patent/CA2243850A1/fr
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8235Fruit-specific

Abstract

La présente invention concerne l'identification et l'isolement de deux régions promoteurs différentes dans le génome des framboises. Dans un génome de framboise natif, ces régions promoteurs sont fonctionnellement liées, à la région de codage du gène drul de la framboise. Les promoteurs de l'invention sont capables de réguler l'expression constitutive à niveau modéré des gènes végétaux hétérologues sous leur influence. L'invention concerne également des gènes chimériques, des vecteurs cassette, des kits, des végétaux transgéniques et des procédés mettant en oeuvre de tels promoteurs.
CA 2243850 1996-01-29 1997-01-28 Promoteurs issus des framboises et permettant l'expression de transgenes dans des vegetaux Abandoned CA2243850A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/592,936 US5783393A (en) 1996-01-29 1996-01-29 Plant tissue/stage specific promoters for regulated expression of transgenes in plants
US08/592,936 1996-01-29
US08/788,928 1997-01-24
US08/788,928 US5783394A (en) 1996-01-29 1997-01-24 Raspberry promoters for expression of transgenes in plants

Publications (1)

Publication Number Publication Date
CA2243850A1 true CA2243850A1 (fr) 1997-07-31

Family

ID=27081576

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2243850 Abandoned CA2243850A1 (fr) 1996-01-29 1997-01-28 Promoteurs issus des framboises et permettant l'expression de transgenes dans des vegetaux

Country Status (5)

Country Link
EP (1) EP0877814A1 (fr)
JP (1) JP2000503847A (fr)
AU (1) AU712253B2 (fr)
CA (1) CA2243850A1 (fr)
WO (1) WO1997027307A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122721B1 (en) 1999-10-05 2006-10-17 Basf Aktiengesellschaft Plant gene expression under the control of constitutive plant V-ATPase promoters
EP1207204A1 (fr) 2000-11-16 2002-05-22 KWS Saat AG Promoteurs spécifiques de tissu de betterave sucrière

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0342926B1 (fr) * 1988-05-17 1994-09-28 Mycogen Plant Science, Inc. Système de promoteur de l'ubiquitine végétale
WO1991001373A1 (fr) * 1989-07-19 1991-02-07 Calgene, Inc. Facteurs de transcription specifiques d'un fruit
US5750864A (en) * 1994-06-17 1998-05-12 Epitope, Inc. Regulated expression of heterologous genes in plants
HU218034B (hu) * 1992-01-09 2000-05-28 Novartis Ag. Új növényi promoter
CA2162925A1 (fr) * 1993-06-18 1995-01-05 Witold Filipowicz Ressources genetiques vegetales chimeriques possedant des sequences regulatrices independantes
US5750870A (en) * 1994-06-17 1998-05-12 Agritope, Inc. Plant genetic transformation methods and transgenic plants

Also Published As

Publication number Publication date
AU1755997A (en) 1997-08-20
AU712253B2 (en) 1999-11-04
EP0877814A1 (fr) 1998-11-18
WO1997027307A1 (fr) 1997-07-31
JP2000503847A (ja) 2000-04-04

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Legal Events

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FZDE Dead