CA2237391A1 - Nouveaux procedes de mise en culture de cellules epitheliales humaines pour l'identification d'une therapeutique et d'un diagnostic du cancer - Google Patents

Nouveaux procedes de mise en culture de cellules epitheliales humaines pour l'identification d'une therapeutique et d'un diagnostic du cancer Download PDF

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Publication number
CA2237391A1
CA2237391A1 CA 2237391 CA2237391A CA2237391A1 CA 2237391 A1 CA2237391 A1 CA 2237391A1 CA 2237391 CA2237391 CA 2237391 CA 2237391 A CA2237391 A CA 2237391A CA 2237391 A1 CA2237391 A1 CA 2237391A1
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CA
Canada
Prior art keywords
cells
cell
culture
epithelial cells
epithelial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2237391
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English (en)
Inventor
Raymond L. White
Stephen Prescott
Leslie Jerominski
Norisada Matsunami
Christine B. Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2237391A1 publication Critical patent/CA2237391A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0631—Mammary cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Abstract

L'invention concerne des procédés d'obtention de cultures primitives de cellules épithéliales du sein, colique et prostatique chez l'homme, à l'aide d'un système de culture tissulaire en co-culture avec du fibroblaste. On peut utiliser les procédés pour obtenir des cultures cellulaires épithéliales normales, précancéreuses et malignes. L'invention concerne également des procédés permettant de dériver des souches des cellules épithéliales sensiblement pures à partir des cultures primitives, et d'immortaliser les souches de cellules afin de produire des lignées cellulaires. Les cultures primitives, les souches de cellules et les lignées cellulaires sont utilisées dans des titrages de criblage et/ou de diagnostic in vitro biochimiques.
CA 2237391 1995-11-15 1996-11-14 Nouveaux procedes de mise en culture de cellules epitheliales humaines pour l'identification d'une therapeutique et d'un diagnostic du cancer Abandoned CA2237391A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55833195A 1995-11-15 1995-11-15
US08/558,331 1995-11-15

Publications (1)

Publication Number Publication Date
CA2237391A1 true CA2237391A1 (fr) 1997-05-22

Family

ID=24229132

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2237391 Abandoned CA2237391A1 (fr) 1995-11-15 1996-11-14 Nouveaux procedes de mise en culture de cellules epitheliales humaines pour l'identification d'une therapeutique et d'un diagnostic du cancer

Country Status (4)

Country Link
EP (1) EP0929661A4 (fr)
AU (1) AU7739296A (fr)
CA (1) CA2237391A1 (fr)
WO (1) WO1997018296A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6074874A (en) * 1997-08-29 2000-06-13 University Of Pittsburgh Epithelial cell cultures for in vitro testing
EP0971232B1 (fr) * 1998-07-05 2004-11-24 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Détermination luminométrique d'ATP
DE60144515D1 (de) 2000-04-01 2011-06-09 Onyvax Ltd Prostata zellinien und deren verwendung
AU2002230768A1 (en) 2000-12-08 2002-06-18 Xgene Corporation In vitro synthesis of a layered cell sorted tissue
WO2003025157A1 (fr) * 2001-09-14 2003-03-27 The Genetics Company Systeme de coculture permettant d'identifier des proteines declenchant la redifferenciation de cellules tumorales
US20050019336A1 (en) 2003-07-23 2005-01-27 Dalgleish Angus George Human prostate cell lines in cancer treatment
DE102011008050A1 (de) * 2011-01-07 2012-07-12 Universitätsklinikum Schleswig-Holstein Verfahren zur Diagnose der familiären adenomatösen Polyposis (FAP)
US10329623B2 (en) * 2015-08-28 2019-06-25 Slmp, Llc Synthetic tissue controls and synthetic tissue microarray controls
CN115322967B (zh) * 2022-06-13 2023-08-08 浙江省人民医院 一种永生化人甲状腺乳头状癌成纤维细胞株及其构建方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4423145A (en) * 1981-05-07 1983-12-27 Stampfer Martha R Enhanced growth medium and method for culturing human mammary epithelial cells
EP0117486A3 (fr) * 1983-02-25 1987-05-13 Sloan-Kettering Institute For Cancer Research Méthode de croissance et de purification de lignées de cellules humaines à partir de populations de cellules hétérogènes de tissu en culture
EP0355112A1 (fr) * 1987-04-22 1990-02-28 Michael Bay Procedes de culture de cellules, et produits
WO1990015862A1 (fr) * 1989-06-12 1990-12-27 Cornell Research Foundation, Inc. Culture in vitro de cellules epitheliales
DK0569527T3 (da) * 1991-01-16 2001-07-16 Syngenta Ltd Nedarvede og somatiske mutationer af APC-gen i colorektal cancer hos mennesker
CA2118015A1 (fr) * 1992-04-14 1993-10-28 Jeffrey R. Marks Methode de detection des tumeurs contenant des complexes de p53 et de hsp70
US5376542A (en) * 1992-04-27 1994-12-27 Georgetown University Method for producing immortalized cell lines using human papilluma virus genes

Also Published As

Publication number Publication date
WO1997018296A1 (fr) 1997-05-22
EP0929661A4 (fr) 2000-01-12
AU7739296A (en) 1997-06-05
EP0929661A1 (fr) 1999-07-21

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