CA2232741A1 - Promoteur du pr-1 d'arabidopsis chimiquement inductible - Google Patents

Promoteur du pr-1 d'arabidopsis chimiquement inductible Download PDF

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Publication number
CA2232741A1
CA2232741A1 CA 2232741 CA2232741A CA2232741A1 CA 2232741 A1 CA2232741 A1 CA 2232741A1 CA 2232741 CA2232741 CA 2232741 CA 2232741 A CA2232741 A CA 2232741A CA 2232741 A1 CA2232741 A1 CA 2232741A1
Authority
CA
Canada
Prior art keywords
seq
promoter
dna molecule
primer
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2232741
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English (en)
Inventor
John Andrew Ryals
Leigh Thorne
Scott Joseph Uknes
Eric Russell Ward
Edouard Guillaume Lebel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2232741A1 publication Critical patent/CA2232741A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention se rapporte à la séquence d'acides nucléiques du promoteur du PR-1 d'Arabidopsis, chimiquement inductible et pleine longueur. Elle se rapporte en outre à des éléments de régulation, agissant en cis, du promoteur du PR-1 d'Arabidopsis intervenant dans l'induction chimique, que l'on a caractérisé au moyen de mutagenèses par délétion et par balayage d'une séquence de liaison et d'empreintes in vivo. On a découvert qu'au moins une partie de la région du promoteur située entre les positions -698 et -621 (relativement au site de démarrage de la transcription du gène PR-1) est nécessaire à l'induction de l'expression génique par des produits chimiques. Deux mutations par balayage d'une séquence de liaison sur 10 paires de bases, centrées au niveau de la paire de bases 640 et au niveau de la paire de bases 610, en amont du site de démarrage de la transcription, suppriment l'inductibilité du promoteur tandis qu'une autre mutation de 10 paires de bases centrée au niveau de la paire de bases 670 engendre des niveaux d'expression induite moyenne quatre fois supérieurs à ceux du promoteur qui n'a pas subi de mutation. Par ailleurs, des empreintes in vivo inductibles sont disposées en positions -629 et -628 et en position -604 sur le brin codant et en position -641 sur le brin non codant. L'invention se rapporte également à l'utilisation de fragments du promoteur du PR-1 d'Arabidopsis, inductibles chimiquement pour réguler l'expression génique dans des plantes en présence de produits chimiques inducteurs du type SA, INA et BTH, ainsi qu'à l'utilisation de ces éléments pour isoler des protéines régulatrices transcriptionnelles intervenant dans la régulation du promoteur et pour synthétiser des promoteurs hybrides inductibles.
CA 2232741 1996-07-23 1997-07-18 Promoteur du pr-1 d'arabidopsis chimiquement inductible Abandoned CA2232741A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2722896P 1996-07-23 1996-07-23
US60/027,228 1996-07-23

Publications (1)

Publication Number Publication Date
CA2232741A1 true CA2232741A1 (fr) 1998-01-29

Family

ID=21836460

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2232741 Abandoned CA2232741A1 (fr) 1996-07-23 1997-07-18 Promoteur du pr-1 d'arabidopsis chimiquement inductible

Country Status (5)

Country Link
EP (1) EP0868426A4 (fr)
JP (1) JPH11513897A (fr)
AU (1) AU708850B2 (fr)
CA (1) CA2232741A1 (fr)
WO (1) WO1998003536A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
WO1999043819A1 (fr) * 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Famille de genes pr-1 et de promoteurs
PL343460A1 (en) 1998-03-11 2001-08-13 Novartis Ag Expression of trehalose biosynthetic genes in plants
US6465636B1 (en) 1998-04-01 2002-10-15 Zeneca Mogen B.V. Pathogen-inducible promoter
GB9813345D0 (en) 1998-06-19 1998-08-19 Nickerson Biocem Ltd Promoters
US8580943B2 (en) 1998-11-12 2013-11-12 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof
US8013138B1 (en) 1998-11-12 2011-09-06 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof
AU2001288478B2 (en) 2000-08-25 2006-11-02 Basf Plant Science Gmbh Plant polynucleotides encoding prenyl proteases
US7456335B2 (en) 2001-09-03 2008-11-25 Basf Plant Science Gmbh Nucleic acid sequences and their use in methods for achieving pathogen resistance in plants
US7705203B2 (en) * 2002-11-12 2010-04-27 Purdue Research Foundation Benzoate inductible promoters
EP1502953B1 (fr) * 2003-07-28 2009-01-28 Universität Hohenheim Promoteurs chimiquement inductibles pour controler l'expression des proteines dans des plantes
EP1730287B1 (fr) * 2004-03-12 2011-05-25 Syngenta Participations AG Promoteurs inductibles
NZ565631A (en) 2005-08-03 2011-01-28 Adelaide Res & Innovation Pty Polysaccharide synthases
CA2628505A1 (fr) 2005-11-08 2007-05-18 Basf Plant Science Gmbh Utilisation de polynucleotides a repetition armadillo (arm1) pour obtenir une resistance elevees aux agents pathogenes chez des vegetaux
ES2464315T3 (es) 2006-01-12 2014-06-02 Basf Plant Science Gmbh Uso de polinucleótidos de estomatina (STM1) para alcanzar una resistencia a patógenos en plantas
DE102006029129A1 (de) 2006-06-22 2007-12-27 Kws Saat Ag Pathogen induzierbarer synthetischer Promotor
WO2008043826A1 (fr) 2006-10-12 2008-04-17 Basf Plant Science Gmbh Procédé d'augmentation de la résistance aux pathogènes de plantes transgéniques
EP2202314B1 (fr) 2007-01-15 2014-03-12 BASF Plant Science GmbH Utilisation de polynucléotides de la subtilisine (RNR9) pour obtenir une résistance à un pathogène dans les plantes
BR112012016290A2 (pt) 2009-12-31 2015-09-01 Pioneer Hi Bred Int Ácido nucléico isolado ou recombinante, cassete de expressão, célula hospedeira não humana, planta e semente transgênica, variante de polipeptídeo oxox isolado ou recombinante, método de modulação do nível de proteina oxalato oxidase (oxox) em uma planta ou célula vegetal, método para aumentar a resistência de uma planta a um patógeno, planta resistente a patógeno, método para identificar variantes oxox com atividade de oxox mantida ou aumentada, método para gerar uma planta que tem resistência aumentada a uma patógeno

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof

Also Published As

Publication number Publication date
JPH11513897A (ja) 1999-11-30
EP0868426A4 (fr) 2002-04-17
AU708850B2 (en) 1999-08-12
WO1998003536A1 (fr) 1998-01-29
AU3804897A (en) 1998-02-10
EP0868426A1 (fr) 1998-10-07

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