CA2231330A1 - Dosage a rendement eleve utilisant des proteines hybrides - Google Patents

Dosage a rendement eleve utilisant des proteines hybrides Download PDF

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Publication number
CA2231330A1
CA2231330A1 CA 2231330 CA2231330A CA2231330A1 CA 2231330 A1 CA2231330 A1 CA 2231330A1 CA 2231330 CA2231330 CA 2231330 CA 2231330 A CA2231330 A CA 2231330A CA 2231330 A1 CA2231330 A1 CA 2231330A1
Authority
CA
Canada
Prior art keywords
fusion protein
recited
protein
binding
expression vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2231330
Other languages
English (en)
Inventor
Alice Marcy
Scott P. Salowe
Douglas Wisniewski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9605210.5A external-priority patent/GB9605210D0/en
Application filed by Individual filed Critical Individual
Publication of CA2231330A1 publication Critical patent/CA2231330A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9493—Immunosupressants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

L'invention porte sur un dosage à rendement élevé utilisé pour sélectionner des composés capables de se lier à une protéine hybride composée d'une protéine-cible et d'une protéine fixant FK506. L'invention concerne également le procédé de préparation de l'ADN codant la protéine hybride, et d'expression de cet ADN. L'invention se rapporte également aux séquences protéiques et d'ADN de recombinaison pour plusieurs protéines hybrides.
CA 2231330 1995-09-15 1996-09-11 Dosage a rendement eleve utilisant des proteines hybrides Abandoned CA2231330A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US381995P 1995-09-15 1995-09-15
US60/003,819 1995-09-15
GBGB9605210.5A GB9605210D0 (en) 1996-03-12 1996-03-12 A high throughput assay using fusion proteins
GB9605210.5 1996-03-12

Publications (1)

Publication Number Publication Date
CA2231330A1 true CA2231330A1 (fr) 1997-03-20

Family

ID=26308918

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2231330 Abandoned CA2231330A1 (fr) 1995-09-15 1996-09-11 Dosage a rendement eleve utilisant des proteines hybrides

Country Status (4)

Country Link
EP (1) EP0871648A1 (fr)
JP (1) JPH11513246A (fr)
CA (1) CA2231330A1 (fr)
WO (1) WO1997010253A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039326A2 (fr) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. Dosage in vitro a polarisation de fluorescence
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
US7094757B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
JP4168028B2 (ja) * 2002-06-25 2008-10-22 積水化学工業株式会社 発現ベクター、宿主、融合タンパク質、融合タンパク質の製造方法及びタンパク質の製造方法
JP4871960B2 (ja) 2006-01-03 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー 優れたシャペロン活性およびフォールディング活性を有するキメラ融合タンパク質
JP2022553409A (ja) * 2019-10-23 2022-12-22 スティヒティング ヘット ネーデルランズ カンケル インスティテュート－アントニ ファン レーウェンフーク ジーケンハウス 免疫細胞制御用キメラポリペプチド

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69230433D1 (de) * 1991-01-18 2000-01-20 Univ New York Cdns-klonierungsverfahren für rezeptortyrosinkinasezielprotein und hgrb-proteine
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
US5498597A (en) * 1992-01-17 1996-03-12 Dana-Farber Cancer Institute, Inc. FKBP-13, an FK506-binding immunophilin
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5464745A (en) * 1993-03-31 1995-11-07 Novagen, Inc. Protein ligand binding region mapping system
US5580979A (en) * 1994-03-15 1996-12-03 Trustees Of Tufts University Phosphotyrosine peptidomimetics for inhibiting SH2 domain interactions

Also Published As

Publication number Publication date
WO1997010253A1 (fr) 1997-03-20
JPH11513246A (ja) 1999-11-16
EP0871648A1 (fr) 1998-10-21

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