CA2231330A1 - Dosage a rendement eleve utilisant des proteines hybrides - Google Patents
Dosage a rendement eleve utilisant des proteines hybrides Download PDFInfo
- Publication number
- CA2231330A1 CA2231330A1 CA 2231330 CA2231330A CA2231330A1 CA 2231330 A1 CA2231330 A1 CA 2231330A1 CA 2231330 CA2231330 CA 2231330 CA 2231330 A CA2231330 A CA 2231330A CA 2231330 A1 CA2231330 A1 CA 2231330A1
- Authority
- CA
- Canada
- Prior art keywords
- fusion protein
- recited
- protein
- binding
- expression vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9493—Immunosupressants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention porte sur un dosage à rendement élevé utilisé pour sélectionner des composés capables de se lier à une protéine hybride composée d'une protéine-cible et d'une protéine fixant FK506. L'invention concerne également le procédé de préparation de l'ADN codant la protéine hybride, et d'expression de cet ADN. L'invention se rapporte également aux séquences protéiques et d'ADN de recombinaison pour plusieurs protéines hybrides.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US381995P | 1995-09-15 | 1995-09-15 | |
US60/003,819 | 1995-09-15 | ||
GBGB9605210.5A GB9605210D0 (en) | 1996-03-12 | 1996-03-12 | A high throughput assay using fusion proteins |
GB9605210.5 | 1996-03-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2231330A1 true CA2231330A1 (fr) | 1997-03-20 |
Family
ID=26308918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2231330 Abandoned CA2231330A1 (fr) | 1995-09-15 | 1996-09-11 | Dosage a rendement eleve utilisant des proteines hybrides |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0871648A1 (fr) |
JP (1) | JPH11513246A (fr) |
CA (1) | CA2231330A1 (fr) |
WO (1) | WO1997010253A1 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2250067A1 (fr) * | 1996-04-18 | 1997-10-23 | Ariad Pharmaceuticals, Inc. | Dosage in vitro a polarisation de fluorescence |
US6962982B2 (en) | 2001-06-22 | 2005-11-08 | Roche Diagnostics Corporation | Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them |
US7094757B2 (en) | 2001-06-22 | 2006-08-22 | Roche Diagnostics Corporation | Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them |
US20050130259A1 (en) * | 2002-06-25 | 2005-06-16 | Akira Ideno | Expression vector, host, fused protein, process for producing fused protein and process for producing protein |
EP1971684B1 (fr) | 2006-01-03 | 2011-10-05 | Roche Diagnostics GmbH | Proteine de fusion chimerique d'activites chaperone et de repliement superieures |
IL292387A (en) * | 2019-10-23 | 2022-06-01 | Stichting Het Nederlands Kanker Inst Antoni Van Leeuwenhoek Ziekenhuis | A chimeric polypeptide for immune cell regulation |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE187772T1 (de) * | 1991-01-18 | 2000-01-15 | Univ New York | Cdns-klonierungsverfahren für rezeptortyrosinkinasezielprotein und hgrb- proteine |
CA2054602C (fr) * | 1991-10-31 | 2003-04-22 | Anthony Pawson | Methode de dosage d'une substance influant sur un systeme de regulation d'un ligand phosphoryle-sh2 |
US5498597A (en) * | 1992-01-17 | 1996-03-12 | Dana-Farber Cancer Institute, Inc. | FKBP-13, an FK506-binding immunophilin |
GB9210176D0 (en) * | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
US5464745A (en) * | 1993-03-31 | 1995-11-07 | Novagen, Inc. | Protein ligand binding region mapping system |
US5580979A (en) * | 1994-03-15 | 1996-12-03 | Trustees Of Tufts University | Phosphotyrosine peptidomimetics for inhibiting SH2 domain interactions |
-
1996
- 1996-09-11 CA CA 2231330 patent/CA2231330A1/fr not_active Abandoned
- 1996-09-11 JP JP9512071A patent/JPH11513246A/ja active Pending
- 1996-09-11 WO PCT/US1996/014567 patent/WO1997010253A1/fr not_active Application Discontinuation
- 1996-09-11 EP EP96935808A patent/EP0871648A1/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
JPH11513246A (ja) | 1999-11-16 |
EP0871648A1 (fr) | 1998-10-21 |
WO1997010253A1 (fr) | 1997-03-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |