CA2227791A1 - Systeme automatique pour coloration de lames - Google Patents
Systeme automatique pour coloration de lames Download PDFInfo
- Publication number
- CA2227791A1 CA2227791A1 CA002227791A CA2227791A CA2227791A1 CA 2227791 A1 CA2227791 A1 CA 2227791A1 CA 002227791 A CA002227791 A CA 002227791A CA 2227791 A CA2227791 A CA 2227791A CA 2227791 A1 CA2227791 A1 CA 2227791A1
- Authority
- CA
- Canada
- Prior art keywords
- slide
- staining
- station
- cover slip
- storage device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000010186 staining Methods 0.000 title claims abstract description 179
- 238000003860 storage Methods 0.000 claims abstract description 65
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000008569 process Effects 0.000 claims abstract description 16
- 239000012530 fluid Substances 0.000 claims description 44
- 239000011347 resin Substances 0.000 claims description 23
- 229920005989 resin Polymers 0.000 claims description 23
- 238000004891 communication Methods 0.000 claims description 20
- 239000003517 fume Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 11
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 238000012546 transfer Methods 0.000 claims description 6
- 239000002699 waste material Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 239000000853 adhesive Substances 0.000 claims description 2
- 230000001070 adhesive effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 claims 3
- 239000000356 contaminant Substances 0.000 claims 2
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 12
- 238000012545 processing Methods 0.000 description 8
- 230000004913 activation Effects 0.000 description 5
- 238000011109 contamination Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 239000006059 cover glass Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 239000003610 charcoal Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
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- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
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- 238000013459 approach Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
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- 230000002411 adverse Effects 0.000 description 1
- 238000005576 amination reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 230000003134 recirculating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B37/00—Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Cette invention, qui concerne un système automatique pour coloration de lames (10) destiné au traitement de lames colorées, des spécimens relevant du domaine de la cytologie et de l'histologie, par exemple, traite de deux modes de réalisation de ce dispositif de coloration. Le premier est constitué d'un dispositif de stockage de lames (12), d'un appareil de transport de lames (14), d'une première table (16) comportant plusieurs postes de coloration (18), d'un poste de chauffe (64) et d'un boîtier (20). Pour se servir du premier mode de réalisation du système selon cette invention, on dispose, dans un premier temps, les lames colorées dans le dispositif de stockage de lames (12). Les lames sont ensuite retirées du dispositif de stockage et traitées dans les différents postes au moyen de l'appareil de transport (14). Il est possible de modifier légèrement ce premier système afin d'y introduire un appareil à verres de montage. Pour ce faire, on enlève le poste de chauffe (64) que l'on remplace par un distributeur de verres de montage. Cette modification permet de mettre en place un verre de montage sur la lame colorée après le passage par tous les postes du système de coloration de lames.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50635895A | 1995-07-24 | 1995-07-24 | |
| US08/506,358 | 1995-07-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2227791A1 true CA2227791A1 (fr) | 1997-02-06 |
Family
ID=24014265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002227791A Abandoned CA2227791A1 (fr) | 1995-07-24 | 1995-10-18 | Systeme automatique pour coloration de lames |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0866748A4 (fr) |
| KR (1) | KR19990035876A (fr) |
| AU (1) | AU3860395A (fr) |
| CA (1) | CA2227791A1 (fr) |
| WO (1) | WO1997003827A1 (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6076583A (en) * | 1994-07-19 | 2000-06-20 | Fisher Scientific Company | Automated slide staining system |
| CA2376969A1 (fr) | 1999-07-21 | 2001-02-01 | Dako A/S | Procede permettant de reguler la temperature d'un echantillon biologique dans un element porteur solide ou sur cet element |
| DE10144042B4 (de) * | 2001-09-07 | 2006-04-13 | Leica Microsystems Nussloch Gmbh | Bearbeitungsvorrichtung zum Färben und Eindecken von Objektträgern |
| DE10144989B4 (de) | 2001-09-12 | 2007-11-08 | Leica Microsystems Nussloch Gmbh | System zum Färben und Eindecken von Objektträgern |
| KR100633051B1 (ko) | 2005-01-20 | 2006-10-12 | 이명섭 | 슬라이드 파스너용 슬라이더의 코팅방법 및 장치 |
| KR100741523B1 (ko) * | 2006-06-29 | 2007-07-20 | 한경대학교 산학협력단 | 미생물 또는 혈액 내 미생물 자동 염색장치 |
| EP2097730B1 (fr) | 2006-10-30 | 2011-10-19 | Ventana Medical Systems, Inc. | Appareil et procédé à film mince |
| DE102007011329A1 (de) * | 2007-03-08 | 2008-09-11 | Medite Gmbh | Verfahren zum Eindecken von auf Objektträgern angeordneten gefärbten histologischen Präparaten |
| DE102010060825B4 (de) * | 2010-11-26 | 2017-10-19 | Leica Biosystems Nussloch Gmbh | Vorrichtung zur Handhabung von Objektträgern |
| US8388891B2 (en) | 2010-12-28 | 2013-03-05 | Sakura Finetek U.S.A., Inc. | Automated system and method of processing biological specimens |
| KR102154283B1 (ko) * | 2018-06-01 | 2020-09-09 | (의)삼성의료재단 | 자동 검체 염색 장치 |
| EP4107506A4 (fr) | 2020-02-22 | 2024-02-28 | Clarapath Inc | Contrôle de coupe et de qualité en microtomie |
| US11959835B2 (en) | 2020-10-23 | 2024-04-16 | Clarapath, Inc. | Preliminary diagnoses of cut tissue sections |
| US20220404241A1 (en) * | 2021-06-21 | 2022-12-22 | Clarapath, Inc. | Integrated System for Preparation of Pathology Samples |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3823845A (en) * | 1973-07-18 | 1974-07-16 | Reserv A Roll Co | Top loading article dispenser |
| CA1106711A (fr) * | 1976-10-08 | 1981-08-11 | Leighton C. Johnson | Systeme pour l'epandage uniforme d'un liquide sur une surface |
| GB1597954A (en) * | 1977-03-04 | 1981-09-16 | Shandon Southern Prod | Microscope slide coverslipping machine |
| US4092952A (en) * | 1977-08-19 | 1978-06-06 | Wilkie Ronald N | Automatic slide stainer |
| US4190472A (en) * | 1977-09-28 | 1980-02-26 | Alex Slonicki | Automated system for the application of coverglasses on histological and cytological slides |
| US4219529A (en) * | 1977-11-28 | 1980-08-26 | Eastman Kodak Company | Incubator for chemical analyzer |
| JPS59157535A (ja) * | 1983-02-28 | 1984-09-06 | Sankyo Co Ltd | 自動標本封入装置 |
| JPS6011136A (ja) * | 1983-06-30 | 1985-01-21 | Chiyoda Seisakusho:Kk | 顕微鏡標本を染色する方法及び装置 |
| US4946832A (en) * | 1987-03-13 | 1990-08-07 | R.I.T.A. Corporation | Cosmetic base composition with therapeutic properties |
-
1995
- 1995-10-18 EP EP95936871A patent/EP0866748A4/fr not_active Withdrawn
- 1995-10-18 AU AU38603/95A patent/AU3860395A/en not_active Abandoned
- 1995-10-18 CA CA002227791A patent/CA2227791A1/fr not_active Abandoned
- 1995-10-18 KR KR1019980700537A patent/KR19990035876A/ko not_active Application Discontinuation
- 1995-10-18 WO PCT/US1995/013155 patent/WO1997003827A1/fr not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0866748A1 (fr) | 1998-09-30 |
| AU3860395A (en) | 1997-02-18 |
| EP0866748A4 (fr) | 2000-03-01 |
| KR19990035876A (ko) | 1999-05-25 |
| WO1997003827A1 (fr) | 1997-02-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |