CA2227065A1 - Liposomes viraux adeno-associes et leur utilisation pour la transfection de cellules dendritiques pour stimuler l'immunite specifique - Google Patents
Liposomes viraux adeno-associes et leur utilisation pour la transfection de cellules dendritiques pour stimuler l'immunite specifique Download PDFInfo
- Publication number
- CA2227065A1 CA2227065A1 CA002227065A CA2227065A CA2227065A1 CA 2227065 A1 CA2227065 A1 CA 2227065A1 CA 002227065 A CA002227065 A CA 002227065A CA 2227065 A CA2227065 A CA 2227065A CA 2227065 A1 CA2227065 A1 CA 2227065A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- antigen
- tumor
- cell
- plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
- A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Epidemiology (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Pulmonology (AREA)
- Physics & Mathematics (AREA)
- Oncology (AREA)
- Dermatology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Reproductive Health (AREA)
Abstract
L'invention porte sur une composition servant à la manipulation génétique de cellules comprenant un liposome fait d'une matière lipidique et d'une matière virale adéno-associée (AAV). Cette dernière, normalement un plasmide, comporte une ou plusieurs répétitions terminales du génome du virus AAV. L'invention porte également sur des procédés d'introduction d'ADN dans des cellules à l'aide de complexes AAV/liposome. L'ADN est introduit et exprimé dans des cellules souches, dans des lymphocytes T, dans des cellules tumorales primaires, dans des lignées cellulaires tumorales, dans des cellules dendritiques ou autres cellules présentant des antigènes. De telles cellules transfectées sont utilisées à des fins thérapeutiques pour traiter des sujets atteints du cancer ou d'infections microbiennes. Les cellules dendritiques dont l'ADN code pour des antigènes tumoraux et viraux servent au traitement de sujets atteints de tumeurs ou d'infections virales par administration in vivo ou par activation ex vivo de lymphocytes spécifiques d'antigènes suivie de leur administration au patient.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US131295P | 1995-07-21 | 1995-07-21 | |
US60/001,312 | 1995-07-21 | ||
US718495P | 1995-11-01 | 1995-11-01 | |
US60/007,184 | 1995-11-01 | ||
US56628695A | 1995-12-01 | 1995-12-01 | |
US08/566,286 | 1995-12-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2227065A1 true CA2227065A1 (fr) | 1997-02-06 |
Family
ID=27356895
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002227065A Abandoned CA2227065A1 (fr) | 1995-07-21 | 1996-07-19 | Liposomes viraux adeno-associes et leur utilisation pour la transfection de cellules dendritiques pour stimuler l'immunite specifique |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0840622A4 (fr) |
JP (1) | JPH11510046A (fr) |
AU (1) | AU6504596A (fr) |
CA (1) | CA2227065A1 (fr) |
WO (1) | WO1997003703A1 (fr) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6734014B1 (en) | 1996-02-08 | 2004-05-11 | The United States Of America As Represented By The Department Of Health And Human Services | Methods and compositions for transforming dendritic cells and activating T cells |
WO1997029183A2 (fr) * | 1996-02-08 | 1997-08-14 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Procedes et compositions pour transformer des cellules dendritiques et activer des lymphocytes t |
EP0906444A1 (fr) * | 1996-04-19 | 1999-04-07 | Gabriele Pecher | Cellules dendritiques humaines genetiquement transfectees, leur production et leur utilisation, de preference comme vaccins |
JP2001515052A (ja) * | 1997-08-13 | 2001-09-18 | ザ ユーエイビー リサーチ ファンデーション | 遺伝子ベクターの局所施用によるワクチン接種 |
GB2370573A (en) * | 1998-11-18 | 2002-07-03 | Oxford Biomedica Ltd | Poxviral vectors |
WO2000054839A2 (fr) * | 1999-03-15 | 2000-09-21 | Introgen Therapeutics, Inc. | Cellules dendritiques transduites avec un gene du soi de type sauvage suscitant des reponses immunitaires antitumorales puissantes |
CA2309313A1 (fr) * | 1999-08-05 | 2001-02-05 | Board Of Trustees Of The University Of Arkansas | Stimulation de la reponse immunitaire par manipulation des cellules dendritiques humaines |
WO2001027156A1 (fr) * | 1999-10-08 | 2001-04-19 | Curagen Corporation | Proteine du type interleukine 2 et acides nucleiques les codant |
US8128922B2 (en) | 1999-10-20 | 2012-03-06 | Johns Hopkins University | Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen |
GB0007150D0 (en) * | 2000-03-24 | 2000-05-17 | Lamellar Therapeutics Limited | Immunotherapeutic methods and compositions |
JP5087201B2 (ja) | 2000-08-03 | 2012-12-05 | ジョンズ・ホプキンス・ユニバーシティ | 抗原に小胞体シャペロンポリペプチドを連結した分子ワクチン |
US20030113919A1 (en) * | 2001-08-17 | 2003-06-19 | Aventis Pasteur, Ltd. | Immunogenic targets for melanoma |
EP1644048B1 (fr) | 2003-05-05 | 2015-04-29 | Johns Hopkins University | Vaccin a adn anti-cancer faisant appel a des plasmides codant une sequence-signal, un antigene oncoproteine mutant, et une proteine de choc thermique |
GB0322448D0 (en) | 2003-09-25 | 2003-10-29 | Lamellar Therapeutics Ltd | Using lamellar bodies to modify linear biological macro molecules |
WO2006073970A2 (fr) | 2005-01-06 | 2006-07-13 | The Johns Hopkins University | Interference arn bloquant l'expression de l'immunite potentialisee de proteines pro-apoptotiques induite par adn et des vaccins de cellules dendritiques transfectes |
US8668905B2 (en) | 2005-05-12 | 2014-03-11 | University Of South Florida | P53 vaccines for the treatment of cancers |
US9085638B2 (en) | 2007-03-07 | 2015-07-21 | The Johns Hopkins University | DNA vaccine enhancement with MHC class II activators |
US8877206B2 (en) | 2007-03-22 | 2014-11-04 | Pds Biotechnology Corporation | Stimulation of an immune response by cationic lipids |
WO2008153802A1 (fr) * | 2007-05-29 | 2008-12-18 | Eusa Pharma, Inc. | Traitement ex-vivo du cancer au moyen de psma et d'anticorps de psma |
US9789129B2 (en) | 2008-04-17 | 2017-10-17 | Pds Biotechnology Corporation | Stimulation of an immune response by enantiomers of cationic lipids |
WO2013039989A1 (fr) * | 2011-09-12 | 2013-03-21 | Pds Biotechnology Corporation | Formulations vaccinales particulaires |
WO2013143555A1 (fr) * | 2012-03-26 | 2013-10-03 | Biontech Ag | Formulation d'arn pour immunothérapie |
TWI672149B (zh) | 2012-09-21 | 2019-09-21 | 美商Pds生技公司 | 改良之疫苗組成物及使用方法 |
AU2016354590B2 (en) | 2015-11-13 | 2023-11-23 | Pds Biotechnology Corporation | Lipids as synthetic vectors to enhance antigen processing and presentation ex-vivo in dendritic cell therapy |
CA3056630A1 (fr) | 2017-03-15 | 2018-09-20 | Pandion Therapeutics, Inc. | Immunotolerance ciblee |
US10676516B2 (en) | 2017-05-24 | 2020-06-09 | Pandion Therapeutics, Inc. | Targeted immunotolerance |
US10174092B1 (en) | 2017-12-06 | 2019-01-08 | Pandion Therapeutics, Inc. | IL-2 muteins |
US10946068B2 (en) | 2017-12-06 | 2021-03-16 | Pandion Operations, Inc. | IL-2 muteins and uses thereof |
WO2020037303A1 (fr) * | 2018-08-16 | 2020-02-20 | The Regents Of The University Of California | Boursouflure de membrane cellulaire initiée chimiquement et photochimiquement pour induire la production de vésicules cellulaires, modifications associées, et utilisations associées |
KR20220035333A (ko) | 2019-05-20 | 2022-03-22 | 팬디온 오퍼레이션스, 인코포레이티드 | Madcam 표적 면역관용 |
US11981715B2 (en) | 2020-02-21 | 2024-05-14 | Pandion Operations, Inc. | Tissue targeted immunotolerance with a CD39 effector |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5126132A (en) * | 1989-08-21 | 1992-06-30 | The United States Of America As Represented By The Department Of Health And Human Services | Tumor infiltrating lymphocytes as a treatment modality for human cancer |
US5252479A (en) * | 1991-11-08 | 1993-10-12 | Research Corporation Technologies, Inc. | Safe vector for gene therapy |
US5587308A (en) * | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
WO1994021287A1 (fr) * | 1993-03-15 | 1994-09-29 | The Government Of The United States Of America As Represented By The Department Of Health And Human Services | Cellules dendritiques enrobees de peptide utilisees en tant qu'immunogenes |
US5834441A (en) * | 1993-09-13 | 1998-11-10 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Adeno-associated viral (AAV) liposomes and methods related thereto |
WO1997029183A2 (fr) * | 1996-02-08 | 1997-08-14 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Procedes et compositions pour transformer des cellules dendritiques et activer des lymphocytes t |
-
1996
- 1996-07-19 EP EP96924646A patent/EP0840622A4/fr not_active Withdrawn
- 1996-07-19 JP JP9506920A patent/JPH11510046A/ja not_active Ceased
- 1996-07-19 AU AU65045/96A patent/AU6504596A/en not_active Abandoned
- 1996-07-19 CA CA002227065A patent/CA2227065A1/fr not_active Abandoned
- 1996-07-19 WO PCT/US1996/012012 patent/WO1997003703A1/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0840622A1 (fr) | 1998-05-13 |
EP0840622A4 (fr) | 2003-01-02 |
AU6504596A (en) | 1997-02-18 |
JPH11510046A (ja) | 1999-09-07 |
WO1997003703A1 (fr) | 1997-02-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2227065A1 (fr) | Liposomes viraux adeno-associes et leur utilisation pour la transfection de cellules dendritiques pour stimuler l'immunite specifique | |
US5834441A (en) | Adeno-associated viral (AAV) liposomes and methods related thereto | |
Cao et al. | Lymphotactin gene-modified bone marrow dendritic cells act as more potent adjuvants for peptide delivery to induce specific antitumor immunity | |
Irvine et al. | Efficient nonviral transfection of dendritic cells and their use for in vivo immunization | |
EP1012240B1 (fr) | Immunotherapie anticancer a base de cellules semi-allogeniques | |
US5962320A (en) | Engineered antigen presenting cells and methods for their use | |
Prince | Gene transfer: a review of methods and applications | |
JP2002515734A (ja) | 遺伝子改変された樹状細胞により媒介される免疫刺激 | |
Herrmann | Cancer gene therapy: principles, problems, and perspectives | |
Tsang et al. | The infection of human dendritic cells with recombinant avipox vectors expressing a costimulatory molecule transgene (CD80) to enhance the activation of antigen-specific cytolytic T cells | |
Van Tendeloo et al. | Gene-based cancer vaccines: an ex vivo approach | |
Philip et al. | Dendritic cells loaded with MART-1 peptide or infected with adenoviral construct are functionally equivalent in the induction of tumor-specific cytotoxic T lymphocyte responses in patients with melanoma | |
US20190125848A1 (en) | Dendritic cell-extracellular vesicle fusions and methods of using same | |
EP1214437A1 (fr) | Utilisation de vecteurs lentiviraux pour la presentation de l'antigene dans les cellules dendritiques | |
Philip et al. | Gene modification of primary tumor cells for active immunotherapy of human breast and ovarian cancer. | |
CA2309313A1 (fr) | Stimulation de la reponse immunitaire par manipulation des cellules dendritiques humaines | |
US6652850B1 (en) | Adeno-associated viral liposomes and their use in transfecting dendritic cells to stimulate specific immunity | |
EP0733373A2 (fr) | Compositions et procédés pour augmenter l'immunogénicité des cellules tumorales par l'administration de cellules transformées pour l'expression de B7 et CD2 protéines | |
AU2004220552A1 (en) | IL-2 transmembrane constructs | |
Lander et al. | Development and in vitro characterization of a GM-CSF secreting human ovarian carcinoma tumor vaccine | |
O’MALLEY JR et al. | Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University, PO Box 41402, Baltimore, MD 21203–6402, USA | |
Humar | Immunotherapy of cancer: protective immunization against tumor cell growth with a mutated p53 allele | |
Jaffee | Methods for Retrovirus-Mediated Gene Transfer to Tumor Cells | |
Rolland | GENE THERAPY FOR CANCER: STRATEGIES AND REVIEW OF CLINICAL TRIALS | |
Ponsaerts | Dendritic cell vaccines for immunity and tolerance: mRNA electroporation as a tool for efficient gene transfer |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |