CA2226427C - Extraction of triglycerides from microorganisms - Google Patents

Extraction of triglycerides from microorganisms Download PDF

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Publication number
CA2226427C
CA2226427C CA002226427A CA2226427A CA2226427C CA 2226427 C CA2226427 C CA 2226427C CA 002226427 A CA002226427 A CA 002226427A CA 2226427 A CA2226427 A CA 2226427A CA 2226427 C CA2226427 C CA 2226427C
Authority
CA
Canada
Prior art keywords
solvent
microorganisms
disintegrated
triglyceride oil
phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002226427A
Other languages
English (en)
French (fr)
Other versions
CA2226427A1 (en
Inventor
John Macdonald Liddell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CA2226427A1 publication Critical patent/CA2226427A1/en
Application granted granted Critical
Publication of CA2226427C publication Critical patent/CA2226427C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • General Chemical & Material Sciences (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002226427A 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms Expired - Lifetime CA2226427C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9514649.4A GB9514649D0 (en) 1995-07-18 1995-07-18 Extraction of triglycerides from microorganisms
GB9514649.4 1995-07-18
PCT/GB1996/001703 WO1997004121A1 (en) 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms

Publications (2)

Publication Number Publication Date
CA2226427A1 CA2226427A1 (en) 1997-02-06
CA2226427C true CA2226427C (en) 2009-10-13

Family

ID=10777825

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002226427A Expired - Lifetime CA2226427C (en) 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms

Country Status (11)

Country Link
US (1) US6180376B1 (https=)
EP (1) EP0843733B1 (https=)
JP (1) JP2001507379A (https=)
AT (1) ATE201907T1 (https=)
AU (1) AU6467096A (https=)
CA (1) CA2226427C (https=)
DE (1) DE69613228T2 (https=)
DK (1) DK0843733T3 (https=)
ES (1) ES2158323T3 (https=)
GB (1) GB9514649D0 (https=)
WO (1) WO1997004121A1 (https=)

Families Citing this family (36)

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Publication number Priority date Publication date Assignee Title
RU2336307C2 (ru) 2000-01-19 2008-10-20 Мартек Биосайнсис Корпорейшн Способ получения липидов (варианты) и липиды, полученные этим способом
EP1178118A1 (en) * 2000-08-02 2002-02-06 Dsm N.V. Isolation of microbial oils
TWI240729B (en) 2000-11-24 2005-10-01 Dow Corning Process for making silicone emulsions
ES2626018T3 (es) 2005-06-07 2017-07-21 Dsm Nutritional Products Ag Microorganismos eucariotas para la producción de lípidos y antioxidantes
WO2006136539A1 (en) 2005-06-23 2006-12-28 Dsm Ip Assets B.V. Process for obtaining lipid from cells
CN101981201A (zh) 2006-08-01 2011-02-23 加拿大海洋营养食品有限公司 产油微生物及改良这些微生物的方法
WO2008151373A1 (en) * 2007-06-14 2008-12-18 Fuji Fuels Pty Ltd Algae growth for biofuels
EP2145942A1 (de) * 2008-07-15 2010-01-20 Lonza Ltd. Verfahren zur Isolierung von Ölen aus Zellen und Biomasse
US9296985B2 (en) * 2009-03-10 2016-03-29 Valicor, Inc. Algae biomass fractionation
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
WO2011090493A1 (en) 2010-01-19 2011-07-28 Martek Biosciences Corporation Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
US8450111B2 (en) 2010-03-02 2013-05-28 Streamline Automation, Llc Lipid extraction from microalgae using a single ionic liquid
US8303818B2 (en) * 2010-06-24 2012-11-06 Streamline Automation, Llc Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction
WO2011127127A2 (en) 2010-04-06 2011-10-13 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of oil and co-products from oleaginous material
CN103124791B (zh) 2010-06-01 2016-08-24 帝斯曼知识产权资产管理有限公司 从细胞中提取脂质以及由此获得的产品
US9028696B2 (en) 2010-07-26 2015-05-12 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
BR112013002003A2 (pt) 2010-07-26 2019-09-24 Sapphire Energy Inc processo para recuperação de compostos oleaginosos de biomassa
US8906236B2 (en) 2010-07-26 2014-12-09 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds and nutrients from biomass
WO2012153174A2 (en) 2011-05-06 2012-11-15 Ariel-University Research And Development Company, Ltd. Wastewater treatment method and device
HK1198515A1 (en) 2011-07-21 2015-05-15 Dsm Ip Assets B.V. Fatty acid compositions
WO2013153981A1 (ja) 2012-04-10 2013-10-17 花王株式会社 脂肪酸エステルの製造方法
DK2970926T3 (en) 2013-03-13 2018-04-16 Dsm Nutritional Products Ag GENMANIPULATION OF MICROorganisms
PL3082793T3 (pl) 2013-12-20 2020-10-05 Dsm Ip Assets B.V. Sposoby otrzymywania oleju drobnoustrojowego z komórek drobnoustrojowych
KR102416853B1 (ko) * 2013-12-20 2022-07-05 마라 리뉴어블즈 코퍼레이션 미생물로부터 오일을 회수하는 방법
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
JP6919827B2 (ja) 2013-12-20 2021-08-18 ディーエスエム ニュートリショナル プロダクツ アーゲーＤＳＭ Ｎｕｔｒｉｔｉｏｎａｌ Ｐｒｏｄｕｃｔｓ ＡＧ 微生物から油を回収する方法
NZ721409A (en) 2013-12-20 2022-10-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
WO2015095693A2 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
SG11201605043TA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
US20160002521A1 (en) * 2014-07-03 2016-01-07 Solazyme, Inc. Lubricants and wellbore fluids
MX2017012103A (es) 2015-03-24 2018-11-12 Terravia Holdings Inc Composiciones de microalgas y sus usos.
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
CA2991707C (en) 2015-07-13 2023-08-01 MARA Renewables Corporation Enhancing microbial metabolism of c5 organic carbon
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
MX2020013907A (es) 2018-06-29 2021-03-09 Amyris Inc Star Metodos para recuperar compuestos isoprenoides inmiscibles en agua a partir de biomasa microbiana.
US20220306964A1 (en) * 2019-06-14 2022-09-29 The University Of Melbourne Shear-induced phase inversion of complex emulsions for recovery of organic components from biomass

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1089093A (en) * 1963-10-03 1967-11-01 British Petroleum Co Improvements in or relating to the production of derivatives from micro-organisms
IL57712A (en) * 1979-07-03 1984-02-29 Yissum Res Dev Co Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product
JPS6012036B2 (ja) * 1982-08-06 1985-03-29 財団法人工業開発研究所 ケイソウから油状物質を抽出する方法
US4870011A (en) * 1985-01-22 1989-09-26 Director General Of Agency Of Industrial Science And Technology Method for obtaining lipids from fungus bodies
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
PH11992043811B1 (en) 1991-01-24 2002-08-22 Martek Corp Arachidonic acid and methods for the production and use thereof
EP0522470B1 (en) 1991-07-11 1996-05-22 Idemitsu Petrochemical Co., Ltd. Triglyceride-containing dry cell fragments and method of preparing them

Also Published As

Publication number Publication date
DK0843733T3 (da) 2001-09-03
ATE201907T1 (de) 2001-06-15
CA2226427A1 (en) 1997-02-06
AU6467096A (en) 1997-02-18
EP0843733B1 (en) 2001-06-06
EP0843733A1 (en) 1998-05-27
JP2001507379A (ja) 2001-06-05
DE69613228D1 (de) 2001-07-12
GB9514649D0 (en) 1995-09-13
WO1997004121A1 (en) 1997-02-06
DE69613228T2 (de) 2001-10-11
US6180376B1 (en) 2001-01-30
ES2158323T3 (es) 2001-09-01

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Effective date: 20160718