CA2218728C - Compositions et procedes d'analyse rapide des reticulocytes - Google Patents

Compositions et procedes d'analyse rapide des reticulocytes Download PDF

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Publication number
CA2218728C
CA2218728C CA002218728A CA2218728A CA2218728C CA 2218728 C CA2218728 C CA 2218728C CA 002218728 A CA002218728 A CA 002218728A CA 2218728 A CA2218728 A CA 2218728A CA 2218728 C CA2218728 C CA 2218728C
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Canada
Prior art keywords
reagent
sample
reticulocytes
methyl
reticulocyte
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Expired - Lifetime
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CA002218728A
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English (en)
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CA2218728A1 (fr
Inventor
Young Ran Kim
Johanna Kantor
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Abbott Laboratories
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Abbott Laboratories
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Priority claimed from US08/426,408 external-priority patent/US5691204A/en
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Publication of CA2218728A1 publication Critical patent/CA2218728A1/fr
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Publication of CA2218728C publication Critical patent/CA2218728C/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention se rapporte à un procédé et à un réactif utilisés dans la numération simultanée ou indépendante des réticulocytes dans un échantillon de sang entier sans qu'il soit nécessaire d'incuber séparément l'échantillon et le réactif. Le réactif contient une quantité d'un colorant de cyanine asymétrique pour réticulocytes, entre environ 40 mM et environ 60 mM d'un tampon sélectionné dans le groupe constitué par imidazole, Tris et Bis-Tris et une quantité apte à stabiliser le colorant d'un tensioactif non ionique sélectionné dans le groupe constitué par N, N-bis[3-D-Glucon-amidopropyl] cholamide et d'un copolymère en bloc de polyoxypropylène-polyoxyéthylyène. Le réactif a un pH compris entre environ 6,8 et environ 7,2 et une osmolarité ajustée entre environ 280 et environ 310 mOsm/l avec un sel alcalin monovalent ou bivalent sélectionné dans le groupe constitué par NaCl, KCl, LiCl, CaCl2, MgCl2 et ZnCl2. Le procédé met en oeuvre le réactif dans un processus sans incubation qui permet également de déterminer simultanément la numération globulaire (CBC) ainsi que des numérations réticulocytaires et des indices de maturité.
CA002218728A 1995-04-21 1996-04-19 Compositions et procedes d'analyse rapide des reticulocytes Expired - Lifetime CA2218728C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/426,408 1995-04-21
US08/426,408 US5691204A (en) 1995-04-21 1995-04-21 Compositions and methods for the rapid analysis of reticulocytes
PCT/US1996/005520 WO1996033396A1 (fr) 1995-04-21 1996-04-19 Compositions et procedes d'analyse rapide des reticulocytes

Publications (2)

Publication Number Publication Date
CA2218728A1 CA2218728A1 (fr) 1996-10-24
CA2218728C true CA2218728C (fr) 2007-07-03

Family

ID=38249462

Family Applications (1)

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CA002218728A Expired - Lifetime CA2218728C (fr) 1995-04-21 1996-04-19 Compositions et procedes d'analyse rapide des reticulocytes

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CA (1) CA2218728C (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019206310A1 (fr) * 2018-04-28 2019-10-31 深圳迈瑞生物医疗电子股份有限公司 Procédé d'analyse de sang, système d'analyse de sang et support de stockage

Also Published As

Publication number Publication date
CA2218728A1 (fr) 1996-10-24

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