CA2214837C - Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique - Google Patents

Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique Download PDF

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Publication number
CA2214837C
CA2214837C CA002214837A CA2214837A CA2214837C CA 2214837 C CA2214837 C CA 2214837C CA 002214837 A CA002214837 A CA 002214837A CA 2214837 A CA2214837 A CA 2214837A CA 2214837 C CA2214837 C CA 2214837C
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CA
Canada
Prior art keywords
resin
anion exchange
purification
deae
immobilized metal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002214837A
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English (en)
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CA2214837A1 (fr
Inventor
Paul W. Shabram
Bernard G. Huyghe
Xiaodong Liu
H. Michael Shepard
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Canji Inc
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Canji Inc
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Publication date
Priority claimed from US08/400,793 external-priority patent/US5837520A/en
Application filed by Canji Inc filed Critical Canji Inc
Priority to CA002522630A priority Critical patent/CA2522630C/fr
Publication of CA2214837A1 publication Critical patent/CA2214837A1/fr
Application granted granted Critical
Publication of CA2214837C publication Critical patent/CA2214837C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Cette invention concerne un procédé de purification de vecteurs viraux contenant des gènes thérapeutiques utilisés en thérapie génétique. Cette invention consiste en un procédé de purification à partir d'un lysat cellulaire d'un vecteur viral recombinant contenant un gène thérapeutique, lequel procédé comporte les étapes suivantes: a) le lysat est traité par un agent enzymatique qui dégrade sélectivement l'ADN et l'ARN non encapsulés; b) le lysat traité en a) est ensuite chromatographié sur une première résine; c) l'éluant obtenu en b) est à son tour chromatographié sur une seconde résine. L'une des résines est une résine permettant l'échange d'anions tandis que la seconde est une résine de chromatographie d'ions métalliques immobilisés (IMAC).
CA002214837A 1995-03-07 1996-03-06 Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique Expired - Fee Related CA2214837C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002522630A CA2522630C (fr) 1995-03-07 1996-03-06 Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/400,793 1995-03-07
US08/400,793 US5837520A (en) 1995-03-07 1995-03-07 Method of purification of viral vectors
PCT/US1996/003369 WO1996027677A2 (fr) 1995-03-07 1996-03-06 Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002522630A Division CA2522630C (fr) 1995-03-07 1996-03-06 Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique

Publications (2)

Publication Number Publication Date
CA2214837A1 CA2214837A1 (fr) 1996-09-12
CA2214837C true CA2214837C (fr) 2005-12-13

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CA002214837A Expired - Fee Related CA2214837C (fr) 1995-03-07 1996-03-06 Procede de purification de vecteurs viraux recombinants contenant un gene therapeutique

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CA (1) CA2214837C (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008298A1 (fr) 1995-08-30 1997-03-06 Genzyme Corporation Purification d'adenovirus et de virus adeno-associe (aav) par voie chromatographique

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Publication number Publication date
CA2214837A1 (fr) 1996-09-12

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