CA2205657A1 - Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales - Google Patents
Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetalesInfo
- Publication number
- CA2205657A1 CA2205657A1 CA 2205657 CA2205657A CA2205657A1 CA 2205657 A1 CA2205657 A1 CA 2205657A1 CA 2205657 CA2205657 CA 2205657 CA 2205657 A CA2205657 A CA 2205657A CA 2205657 A1 CA2205657 A1 CA 2205657A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- acp
- seed
- stearoyl
- stearate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
L'invention concerne les thioestérases végétales, en particulier des acyl-PVA thioestérases ayant une activité significative sur la stéaroyl-PVA. On décrit des constructions d'ADN utiles pour l'expression d'une stéaroyl-PVA thioestérase végétale dans des graines de plante. Ces constructions vont contenir une séquence d'ADN codant pour la stéaroyl-PVA thioestérase végétale choisie sous le contrôle d'éléments régulateurs capables de diriger préférentiellement l'expression de la stéaroyl-PVA thioestérase végétale vers des tissus de graines, plutôt que d'autres tissus de plante, quand une telle construction est exprimée dans une plante transgénique. Cette invention concerne également des procédés d'utilisation d'une séquence d'ADN codant une stéaroyl-PVA thioestérase de plante pour la modification d'un stéarate produit dans une cellule de graine de plante. Une thioestérase de plante ayant une activité significative sur la stéaroyl-PVA donnée ici comme exemple vient de mangoustan. La concentration modifiée de stéarate peut être obtenue par l'expression de la thioestérase de mangoustan seule ou en combinaison avec des constructions antisense diminuant la stéaroyl-PVA désaturase dans les graines de plantes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US53708395A | 1995-09-29 | 1995-09-29 | |
US08/537,083 | 1995-09-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2205657A1 true CA2205657A1 (fr) | 1997-04-03 |
Family
ID=24141133
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2205657 Abandoned CA2205657A1 (fr) | 1995-09-29 | 1996-09-30 | Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0795020A4 (fr) |
JP (1) | JPH10510438A (fr) |
CA (1) | CA2205657A1 (fr) |
WO (1) | WO1997012047A1 (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6150512A (en) * | 1995-05-15 | 2000-11-21 | Yuan; Ling | Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity |
AU7147498A (en) | 1997-05-05 | 1998-11-27 | Dow Agrosciences Llc | Nucleotide sequences of maize oleoyl-acp thioesterase and palmitoyl-acp thioesterase genes and their use in the modification of fatty acid content of oil |
CA2328449A1 (fr) * | 1998-07-21 | 2000-02-03 | Dow Agrosciences Llc | Regulation negative de proteines vegetales a mediation par anticorps |
US6365802B2 (en) | 1998-08-14 | 2002-04-02 | Calgene Llc | Methods for increasing stearate content in soybean oil |
US6713117B1 (en) | 1998-10-02 | 2004-03-30 | Dharma Kodali | Vegetable oil having elevated stearic acid content |
US7592015B2 (en) | 1999-06-04 | 2009-09-22 | Consejo Superior De Investigaciones Cientificas | Use of high oleic high stearic oils |
EP1554925A1 (fr) | 1999-06-04 | 2005-07-20 | Consejo Superior de Investigaciones Cientificas | Produits alimentaires et cosmétiques renfermant des huiles à forte teneur en acide oléique et forte teneur en acide stéarique |
US6348610B1 (en) | 1999-06-04 | 2002-02-19 | Consejo Superior De Investigaciones Cientificas (Csic) | Oil from seeds with a modified fatty acid composition |
US6388113B1 (en) | 1999-06-04 | 2002-05-14 | Consejo Superior De Investigaciones Cientificas ( Csic) | High oleic/high stearic sunflower oils |
CN101213298B (zh) * | 2005-06-30 | 2012-04-18 | 丰田自动车株式会社 | 参与岩芹酸生物合成的新基因和用于生产岩芹酸的方法 |
ES2730713T3 (es) | 2008-12-23 | 2019-11-12 | Reg Life Sciences Llc | Métodos y composiciones relacionados con enzimas tioesterasas |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5344771A (en) * | 1990-04-26 | 1994-09-06 | Calgene, Inc. | Plant thiosterases |
US5512482A (en) * | 1990-04-26 | 1996-04-30 | Calgene, Inc. | Plant thioesterases |
DE69113635T3 (de) * | 1990-12-20 | 2000-06-08 | Du Pont | Nukleotidsequenzen der sojabohnen-acyl-acp-thioesterasegene. |
US5455167A (en) * | 1991-05-21 | 1995-10-03 | Calgene Inc. | Medium-chain thioesterases in plants |
GB9204583D0 (en) * | 1992-03-03 | 1992-04-15 | Unilever Plc | Recombinant plant enzyme |
CA2176137A1 (fr) * | 1993-11-10 | 1995-05-18 | Alois Toni Voelker | Sequences de thioesterases vegetales actives sur les substrats acyl-(proteine vectrice d'acyle) |
CA2206984A1 (fr) * | 1995-05-15 | 1996-11-21 | Calgene, Inc. | Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat |
-
1996
- 1996-09-30 CA CA 2205657 patent/CA2205657A1/fr not_active Abandoned
- 1996-09-30 WO PCT/US1996/016078 patent/WO1997012047A1/fr not_active Application Discontinuation
- 1996-09-30 JP JP9513773A patent/JPH10510438A/ja active Pending
- 1996-09-30 EP EP96936250A patent/EP0795020A4/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
EP0795020A4 (fr) | 1999-02-24 |
JPH10510438A (ja) | 1998-10-13 |
WO1997012047A1 (fr) | 1997-04-03 |
EP0795020A1 (fr) | 1997-09-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |