CA2200496C - Transformation genetique a l'aide d'un inhibiteur de la parp - Google Patents
Transformation genetique a l'aide d'un inhibiteur de la parp Download PDFInfo
- Publication number
- CA2200496C CA2200496C CA2200496A CA2200496A CA2200496C CA 2200496 C CA2200496 C CA 2200496C CA 2200496 A CA2200496 A CA 2200496A CA 2200496 A CA2200496 A CA 2200496A CA 2200496 C CA2200496 C CA 2200496C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- inhibitor
- gene
- plant cells
- foreign dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention se rapporte à un procédé pour obtenir des cellules eucaryotes transgéniques, particulièrement de plantes. Ce procédé consiste à: mettre en contact une culture de cellules non transformées avec un inhibiteur de la poly-(ADP-ribose)polymérase pendant un temps suffisant pour diminuer la réponse des cellules cultivées au stress et pour diminuer leur métabolisme. Ensuite, les cellules non transformées sont mises en contact avec un ADN étranger comportant au moins un gène à étudier dans des conditions dans lesquelles l'ADN étranger est absorbé par les cellules non transformées et le gène à étudier est intégré de façon stable dans le génome nucléaire des cellules non transformées afin de produire des cellules transgéniques. Eventuellement, les cellules transgéniques sont récupérées à partir de la culture. De préférence, l'inhibiteur est l'amide nicotinique, de préférence à une concentration de 200 mg/l à 500 mg/l environ et les cellules non transformées sont cultivées dans un milieu contenant l'inhibiteur pendant une durée d'environ 3 à 14 jours avant d'être mises en contact avec l'ADN étranger. L'invention se rapporte également à une plante comprenant dans le génome nucléaire de ses cellules un ADN étranger intégré uniquement dans les régions du génome nucléaire qui présentent une activité de transcription dans les cellules de la plante lorsque les cellules sont traitées par une quantité efficace d'un inhibiteur de la PARP pendant un temps suffisant pour diminuer le métabolisme cellulaire à un niveau où l'expression génétique est essentiellement limitée à des gènes exprimés, sans tenir compte de la condition différenciée ou physiologique de la cellule.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95401844A EP0757102A1 (fr) | 1995-08-04 | 1995-08-04 | Transformation génétique utilisant un inhibiteur de PARP |
| GB95401844.6 | 1995-08-04 | ||
| PCT/EP1996/003366 WO1997006267A2 (fr) | 1995-08-04 | 1996-07-31 | Transformation genetique par l'intermediaire d'un inhibiteur de la poly-(adp-ribose)polymerase (parp) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2200496A1 CA2200496A1 (fr) | 1997-02-20 |
| CA2200496C true CA2200496C (fr) | 2010-04-20 |
Family
ID=42115799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2200496A Expired - Fee Related CA2200496C (fr) | 1995-08-04 | 1996-07-31 | Transformation genetique a l'aide d'un inhibiteur de la parp |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2200496C (fr) |
-
1996
- 1996-07-31 CA CA2200496A patent/CA2200496C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2200496A1 (fr) | 1997-02-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20130731 |