CA2181547A1 - Procede permettant l'expansion de cellules hematopo?etiques et procedes de greffe associes - Google Patents

Procede permettant l'expansion de cellules hematopo?etiques et procedes de greffe associes

Info

Publication number
CA2181547A1
CA2181547A1 CA002181547A CA2181547A CA2181547A1 CA 2181547 A1 CA2181547 A1 CA 2181547A1 CA 002181547 A CA002181547 A CA 002181547A CA 2181547 A CA2181547 A CA 2181547A CA 2181547 A1 CA2181547 A1 CA 2181547A1
Authority
CA
Canada
Prior art keywords
cells
acs
cartridge
hematopoietic
gly ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002181547A
Other languages
English (en)
Inventor
Thomas A. Davis
Kelvin P. Lee
William R. Kidwell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Cellco Inc
US Department of Navy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellco Inc, US Department of Navy filed Critical Cellco Inc
Publication of CA2181547A1 publication Critical patent/CA2181547A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un procédé permettant l'expansion ex vivo de cellules hématopoïétiques, y compris des cellules souches et mères hématopoïétiques, au moyen de cellules de support endothéliales, dans un système de capillaires artificiel (ACS). L'invention concerne également des procédés améliorés de greffe de moelle osseuse. Est également décrit un procédé permettant la stimulation de l'expansion ex vivo de cellules hématopoïétiques, consistant à revêtir les capillaires du système de capillaires artificiel avec un réactif approprié. L'invention concerne en outre un procédé de transduction ex vivo de cellules hématopoïétiques en expansion, y compris des cellules hématopoïétiques souches et mères, avec des vecteurs rétroviraux de recombinaison à encapsulation.
CA002181547A 1994-01-21 1995-01-20 Procede permettant l'expansion de cellules hematopo?etiques et procedes de greffe associes Abandoned CA2181547A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18414094A 1994-01-21 1994-01-21
US08/184,140 1994-01-21

Publications (1)

Publication Number Publication Date
CA2181547A1 true CA2181547A1 (fr) 1995-07-27

Family

ID=22675717

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002181547A Abandoned CA2181547A1 (fr) 1994-01-21 1995-01-20 Procede permettant l'expansion de cellules hematopo?etiques et procedes de greffe associes

Country Status (4)

Country Link
JP (1) JPH08205860A (fr)
AU (1) AU1685895A (fr)
CA (1) CA2181547A1 (fr)
WO (1) WO1995019793A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928638A (en) * 1996-06-17 1999-07-27 Systemix, Inc. Methods for gene transfer
WO1999000486A1 (fr) * 1997-06-26 1999-01-07 Cli Oncology, Inc. Compositions et procedes permettant d'induire la croissance et la differenciation de cellules souches hematopietiques
AU759719B2 (en) * 1999-02-04 2003-04-17 Pluristem Ltd. Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells
FR2794473B1 (fr) * 1999-06-03 2003-09-26 Centre Nat Rech Scient Procede de multiplication de cellules souches
DE10234192B4 (de) * 2002-07-26 2009-11-26 Epoplus Gmbh Co.Kg Verwendung von Erythropoetin
WO2007136424A2 (fr) * 2006-05-17 2007-11-29 Cognate Therapeutics, Inc. Isolation et purification de cellules souches hématopoïétiques à partir d'aspirâts de liposuccion
US7754486B2 (en) * 2006-12-26 2010-07-13 Institut De Recherche En Hematologie Et Transplantation Culture medium named MV06 for both endothelial and myocardiac cells
AU2009313290B2 (en) * 2008-11-06 2016-04-21 Indiana University Research & Technology Corporation Materials and methods to enhance hematopoietic stem cells engraftment procedures

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220725A (en) * 1978-04-03 1980-09-02 United States Of America Capillary cell culture device

Also Published As

Publication number Publication date
AU1685895A (en) 1995-08-08
WO1995019793A1 (fr) 1995-07-27
JPH08205860A (ja) 1996-08-13

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Legal Events

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FZDE Dead