CA2144601A1 - Recombinant type ii collagenase from clostridium histolyticum and its use for isolating cells and groups of cells - Google Patents

Recombinant type ii collagenase from clostridium histolyticum and its use for isolating cells and groups of cells

Info

Publication number
CA2144601A1
CA2144601A1 CA002144601A CA2144601A CA2144601A1 CA 2144601 A1 CA2144601 A1 CA 2144601A1 CA 002144601 A CA002144601 A CA 002144601A CA 2144601 A CA2144601 A CA 2144601A CA 2144601 A1 CA2144601 A1 CA 2144601A1
Authority
CA
Canada
Prior art keywords
cells
groups
seq
collagenase
cell tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002144601A
Other languages
French (fr)
Other versions
CA2144601C (en
Inventor
Dorothee Ambrosius
Friederike Hesse
Helmut Burtscher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of CA2144601A1 publication Critical patent/CA2144601A1/en
Application granted granted Critical
Publication of CA2144601C publication Critical patent/CA2144601C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • Physiology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Process for disintegrating cell tissue and releasing cells or groups of cells contained therein by incubation of the cell tissue with a class II collagenase from Clostridium histolyticum, which still has a considerable degree of collagenase activity after 12 freeze/thaw cycles and contains SEQ ID NO:2 or SEQ ID NO:10 in a characteristic partial region or it is coded in this region by SEQ ID NO:1, SEQ ID NO:9 or a sequence which codes for the same amino acid sequence within the scope of the degeneracy of the genetic code and is the product of a prokaryotic or eukaryotic expression of an exogenous DNA, until the cells or groups of cells are released to the desired extent and separating the cells or the groups of cells from the cell tissue fractions.
CA002144601A 1994-03-16 1995-03-14 Recombinant type ii collagenase from clostridium histolyticum and its use for isolating cells and groups of cells Expired - Fee Related CA2144601C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4408940A DE4408940A1 (en) 1994-03-16 1994-03-16 Recombinant type II collagenase from Clostridium histolyticum and its use for the isolation of cells and cell assemblies
DEP4408940.6 1994-03-16

Publications (2)

Publication Number Publication Date
CA2144601A1 true CA2144601A1 (en) 1995-09-17
CA2144601C CA2144601C (en) 2002-05-14

Family

ID=6512956

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002144601A Expired - Fee Related CA2144601C (en) 1994-03-16 1995-03-14 Recombinant type ii collagenase from clostridium histolyticum and its use for isolating cells and groups of cells

Country Status (4)

Country Link
EP (1) EP0677586A1 (en)
JP (1) JP2634782B2 (en)
CA (1) CA2144601C (en)
DE (1) DE4408940A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2272244A1 (en) 1996-11-19 1998-05-28 Roche Diagnostics Gmbh Recombinant collagenase type i from clostridium histolyticum and its use for isolating cells and groups of cells
WO1999016865A1 (en) * 1997-09-26 1999-04-08 Novo Nordisk A/S Use of proteases in passaging of adherent animal or human cell cultures
DE10331171A1 (en) 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutral protease (NP) and product using neutral tissue release tissue protease and method for its preparation
US8323642B2 (en) 2006-12-13 2012-12-04 Depuy Mitek, Inc. Tissue fusion method using collagenase for repair of soft tissue
US10071143B1 (en) 2007-05-03 2018-09-11 The Research Foundation For The State University Of New York Methods for non-surgical treatment of carpal tunnel syndrome
US9636385B2 (en) 2012-10-24 2017-05-02 The Research Foundation For The State University Of New York Use of collagenase to treat glaucoma

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5177017A (en) * 1990-03-22 1993-01-05 Trigen, Inc. Molecular cloning of the genes responsible for collagenase production from Clostridium histolyticum
EP0654084A4 (en) * 1992-06-22 1996-07-03 Trigen Inc Molecular cloning of the genes reponsible for collagenase production from clostridium histolyticum.

Also Published As

Publication number Publication date
EP0677586A1 (en) 1995-10-18
JP2634782B2 (en) 1997-07-30
CA2144601C (en) 2002-05-14
DE4408940A1 (en) 1995-10-05
JPH07313159A (en) 1995-12-05

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