WO2000037682A8 - Method for augmenting expression of a foreign gene - Google Patents

Method for augmenting expression of a foreign gene Download PDF

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Publication number
WO2000037682A8
WO2000037682A8 PCT/US1999/030460 US9930460W WO0037682A8 WO 2000037682 A8 WO2000037682 A8 WO 2000037682A8 US 9930460 W US9930460 W US 9930460W WO 0037682 A8 WO0037682 A8 WO 0037682A8
Authority
WO
WIPO (PCT)
Prior art keywords
foreign gene
expression
augmenting
augmenting expression
cultured
Prior art date
Application number
PCT/US1999/030460
Other languages
French (fr)
Other versions
WO2000037682A1 (en
Inventor
Elaine T Schenborn
William Brondyk
Berg Lisa G De
Brian D Almond
Original Assignee
Promega Corp
Elaine T Schenborn
William Brondyk
Berg Lisa G De
Brian D Almond
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp, Elaine T Schenborn, William Brondyk, Berg Lisa G De, Brian D Almond filed Critical Promega Corp
Priority to AU23747/00A priority Critical patent/AU2374700A/en
Publication of WO2000037682A1 publication Critical patent/WO2000037682A1/en
Publication of WO2000037682A8 publication Critical patent/WO2000037682A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for augmenting transient or stable expression of a foreign gene in cultured eukaryotic cells comprises transfecting a host cell with a foreign gene of interest and with a nucleic acid sequence encoding a bHLH protein or active fragment or homolog thereof, such that expression of the foreign gene is either increased or prolonged or both. The method can be used to produce large amounts of a transgenic gene product in cultured cells. Vectors for carrying out the method are also provided.
PCT/US1999/030460 1998-12-21 1999-12-20 Method for augmenting expression of a foreign gene WO2000037682A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU23747/00A AU2374700A (en) 1998-12-21 1999-12-20 Method for augmenting expression of a foreign gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11326198P 1998-12-21 1998-12-21
US60/113,261 1998-12-21

Publications (2)

Publication Number Publication Date
WO2000037682A1 WO2000037682A1 (en) 2000-06-29
WO2000037682A8 true WO2000037682A8 (en) 2000-10-19

Family

ID=22348470

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030460 WO2000037682A1 (en) 1998-12-21 1999-12-20 Method for augmenting expression of a foreign gene

Country Status (2)

Country Link
AU (1) AU2374700A (en)
WO (1) WO2000037682A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10104584A1 (en) * 2001-02-01 2002-08-22 Gsf Forschungszentrum Umwelt Medane genes and proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU642184B2 (en) * 1989-12-21 1993-10-14 University Of Georgia Research Foundation, Inc., The Bovine myogenic factor gene
US5352595A (en) * 1991-09-03 1994-10-04 Fred Hutchinson Cancer Research Center Myod regulatory region
JPH07505534A (en) * 1992-04-10 1995-06-22 フォックス　チェイス　キャンサー　センター Transcriptional control elements for increasing gene expression in myoblasts

Also Published As

Publication number Publication date
AU2374700A (en) 2000-07-12
WO2000037682A1 (en) 2000-06-29

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