CA2114311C - Lignee cellulaire monoclonale de lymphocytes b wb - Google Patents
Lignee cellulaire monoclonale de lymphocytes b wbInfo
- Publication number
- CA2114311C CA2114311C CA002114311A CA2114311A CA2114311C CA 2114311 C CA2114311 C CA 2114311C CA 002114311 A CA002114311 A CA 002114311A CA 2114311 A CA2114311 A CA 2114311A CA 2114311 C CA2114311 C CA 2114311C
- Authority
- CA
- Canada
- Prior art keywords
- protein
- cell line
- cells
- cell
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004027 cell Anatomy 0.000 claims abstract description 39
- 210000003719 b-lymphocyte Anatomy 0.000 claims abstract description 11
- 101000835093 Homo sapiens Transferrin receptor protein 1 Proteins 0.000 claims abstract description 8
- 102100026144 Transferrin receptor protein 1 Human genes 0.000 claims abstract description 8
- 101001018097 Homo sapiens L-selectin Proteins 0.000 claims abstract description 7
- 102100033467 L-selectin Human genes 0.000 claims abstract description 7
- 239000012980 RPMI-1640 medium Substances 0.000 claims abstract description 7
- 102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 claims abstract description 6
- 101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 claims abstract description 6
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 claims abstract description 6
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 claims abstract description 6
- 101000878605 Homo sapiens Low affinity immunoglobulin epsilon Fc receptor Proteins 0.000 claims abstract description 6
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 claims abstract description 6
- 102100038007 Low affinity immunoglobulin epsilon Fc receptor Human genes 0.000 claims abstract description 6
- 210000003000 inclusion body Anatomy 0.000 claims abstract description 6
- 210000003470 mitochondria Anatomy 0.000 claims abstract description 5
- 239000000427 antigen Substances 0.000 claims abstract description 4
- 108091007433 antigens Proteins 0.000 claims abstract description 4
- 102000036639 antigens Human genes 0.000 claims abstract description 4
- 230000001788 irregular Effects 0.000 claims abstract description 4
- 230000010076 replication Effects 0.000 claims abstract description 4
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 claims abstract description 3
- 102100022005 B-lymphocyte antigen CD20 Human genes 0.000 claims abstract description 3
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 claims abstract description 3
- 101000897405 Homo sapiens B-lymphocyte antigen CD20 Proteins 0.000 claims abstract description 3
- 230000004913 activation Effects 0.000 claims abstract description 3
- 238000004114 suspension culture Methods 0.000 claims abstract 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims description 9
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 4
- 102000005613 PAX5 Transcription Factor Human genes 0.000 claims description 4
- 108010045055 PAX5 Transcription Factor Proteins 0.000 claims description 4
- 102000018594 Tumour necrosis factor Human genes 0.000 claims description 2
- 108050007852 Tumour necrosis factor Proteins 0.000 claims description 2
- 239000007640 basal medium Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 229940027941 immunoglobulin g Drugs 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 210000003935 rough endoplasmic reticulum Anatomy 0.000 abstract description 6
- 239000003636 conditioned culture medium Substances 0.000 abstract description 4
- 239000002609 medium Substances 0.000 abstract description 4
- 230000006907 apoptotic process Effects 0.000 abstract description 3
- 238000002135 phase contrast microscopy Methods 0.000 abstract description 3
- 230000035755 proliferation Effects 0.000 abstract description 3
- 230000001086 cytosolic effect Effects 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 230000003442 weekly effect Effects 0.000 abstract description 2
- 238000003917 TEM image Methods 0.000 abstract 4
- 150000001720 carbohydrates Chemical class 0.000 abstract 2
- 238000001493 electron microscopy Methods 0.000 abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 102000040739 Secretory proteins Human genes 0.000 abstract 1
- 108091058545 Secretory proteins Proteins 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000001338 necrotic effect Effects 0.000 abstract 1
- 210000005259 peripheral blood Anatomy 0.000 abstract 1
- 239000011886 peripheral blood Substances 0.000 abstract 1
- 210000003934 vacuole Anatomy 0.000 abstract 1
- 102000004338 Transferrin Human genes 0.000 description 4
- 108090000901 Transferrin Proteins 0.000 description 4
- 239000012581 transferrin Substances 0.000 description 4
- 230000007774 longterm Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 108010033576 Transferrin Receptors Proteins 0.000 description 2
- 102000007238 Transferrin Receptors Human genes 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000012202 endocytosis Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 108010027122 ADP-ribosyl Cyclase 1 Proteins 0.000 description 1
- 102000018667 ADP-ribosyl Cyclase 1 Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108091006975 Iron transporters Proteins 0.000 description 1
- 102000003729 Neprilysin Human genes 0.000 description 1
- 108090000028 Neprilysin Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000004524 haematopoietic cell Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 238000004627 transmission electron microscopy Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0635—B lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002114311A CA2114311C (fr) | 1991-11-25 | 1991-11-25 | Lignee cellulaire monoclonale de lymphocytes b wb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002114311A CA2114311C (fr) | 1991-11-25 | 1991-11-25 | Lignee cellulaire monoclonale de lymphocytes b wb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2114311A1 CA2114311A1 (fr) | 1993-05-26 |
| CA2114311C true CA2114311C (fr) | 1996-02-27 |
Family
ID=4152799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002114311A Expired - Fee Related CA2114311C (fr) | 1991-11-25 | 1991-11-25 | Lignee cellulaire monoclonale de lymphocytes b wb |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2114311C (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6599719B2 (en) | 1994-11-07 | 2003-07-29 | Human Genome Sciences, Inc. | Nucleic acid molecules encoding tumor necrosis factor-gamma-alpha |
| US6824767B2 (en) | 1994-11-07 | 2004-11-30 | Human Genome Sciences, Inc. | Tumor necrosis factor-gamma |
| DE69434988T2 (de) * | 1994-11-07 | 2008-03-06 | Human Genome Sciences, Inc. | Tumornekrose-faktor-gamma |
-
1991
- 1991-11-25 CA CA002114311A patent/CA2114311C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2114311A1 (fr) | 1993-05-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |